FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yu Zhang,Zhiqing Lin,Qi Yao,Jian He,Haotian Feng,Wenyi Zhang,Zhigang Liu,Tian Yuan,Xuebo Liu,Long Ding
This study aimed to investigate the effect of milk peptides on irritable bowel syndrome (IBS). The mice were intragastrally administered with casein or whey protein hydrolysates at a dose of 1 g/kg body weight/day for 24 days and were subjected to Citrobacter rodentium infection and water avoidance stress from day 7 to 24. Results indicated that casein and whey protein hydrolysates effectively reduced diarrhea, anxiety, and visceral hypersensitivity in IBS mice.. Casein and whey protein hydrolysates regulated gut microbiota composition and increased the abundance of short-chain fatty acid-producing bacteria, such as Alloprevotella and Alistipes . Whey protein hydrolysate significantly increased the mRNA levels of zonula occludens-1 (ZO-1) and claudin-1 in the colon, while casein hydrolysate significantly improved the mRNA levels of occludin. Casein and whey protein hydrolysates both decreased the levels of pro-inflammatory cytokines including interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), while increased the level of anti-inflammatory cytokine interleukin-10 (IL-10). Importantly, casein and whey protein hydrolysates significantly reduced colonic mast cell activation and decreased prostaglandin E2 (PGE2) production. Moreover, three novel casein-derived cyclooxygenase-2 (COX2)-inhibitory peptides RGPF, FPK, and NPW were identified with IC 50 values of 0.36 ± 0.03, 0.64 ± 0.01, and 1.10 ± 0.09 mM, respectively and predicted to form hydrogen bonds and hydrophobic interactions with the residues of the active site of COX2. This study highlighted the potential of milk peptides as bioactive ingredients in functional foods for managing IBS.
研究领域:
营养学
·
免疫学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2270-2281
·
IF 3.289
Han Wu,Xin Rui,Wei Li,Yu Xiao,Jianzhong Zhou,Mingsheng Dong
This study explored a novel strategy to develop solid-state whole-grain oats as a novel carrier of lactic acid bacteria and a nutraceutical supplement rich in ACE inhibitory peptides. Oats were fermented by Lactobacillus plantarum B1-6, Rhizopus oryzae, or a combination of L. plantarum B1-6 and R. oryzae. L. plantarum showed a much better growth performance in oats when it was combined with R. oryzae than when it was cultured alone, as evidenced by an increase in viable cell count to 9.70 log cfu g−1 after 72 h of fermentation. The coinoculated fermented oats (CFO) and the R. oryzae-fermented oats (RFO) were then selected for investigations on protein hydrolysis and on the functional properties of the released bioactive peptides. The results showed that the soluble protein contents changed from 7.05 mg g−1 to 14.43 and 10.21 mg g−1 for CFO and RFO, respectively. However, the degree of hydrolysis and the content of peptides with molecular masses less than 10 000 Da indicated that the CFO proteins can be degraded to a greater degree. As analyzed by electrophoresis and reversed-phase high-performance liquid chromatography, the protein and peptide profiles of CFO and RFO demonstrated that the proteins from CFO were more obviously hydrolyzed and more small peptides were obtained. In addition, both CFO and RFO presented higher ACE inhibitory activities than unfermented oats, whereas the protein extracts from CFO exerted a lower IC50 value of 0.42 mg protein per mL compared with the protein extracts from the other samples. This research has broadened our knowledge on the development of whole-grain oat products as a probiotic carrier and on the difference between mixed solid-state fermentation (SSF) and fungi SSF in terms of protein degradation and the capacity to release ACE inhibitory peptides. Our approach could be used to obtain probiotic food products and probably to develop oats as a potential therapeutic ingredient targeting hypertension.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NORDIC PULP & PAPER RESEARCH JOURNAL
·
2018
·Vol. 33
·期 3
·557-567
·
IF 1.131
Mingfu Li,Guangxing Li,Yi Liu,Ye Jin,Kelei Zhang,Lianxin Luo
Fenton’s reagent has strong oxidizing properties and can effectively remove refractory organic pollutants. Fenton’s reagent was applied to deal with the dissolved colloidal substance (DCS) in the white water of old corrugated container (OCC). The optimum reaction condition on Fenton treatment was analyzed by response surface methodology (RSM) based on response values of DCS and COD Cr . The result indicated that the optimized condition is as following, pH is 2.45, a mass ratio of H 2 O 2 and COD Cr is 1.1 and a mole ratio of FeSO 4 and H 2 O 2 is 0.35. Under the optimum condition of a single factor experiment, the experimental results showed that the removal rate of CODcr and DCS was 90.68 % and 1.61 g/L respectively, which was consistent with the response surface analysis prediction values. During the Fenton oxidation, the particle sizes of DCS decreased, the ions content and turbidity of the white water decreased, however the electrical conductivity and zeta potential of the white water raised slightly. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis proved that benzoic acid and nonandioic acid can be effectively removed by Fenton’s reagent, however the content of dehydroabietic acid decreased by 24.07 %.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3468
·
IF 5.2
Chongzhen Sun,Hongyan Li,Xiaodan Hui,Yurong Ma,Zhina Yin,Qingsong Chen,Cong Chen,Hui Wu,Xiyang Wu
Mulberry leaf protein hydrolysates (HMP), and their in vitro gastrointestinal digests (GHMP), have shown favorable chemical antioxidant activities. The aim of this study is to investigate the potential protective effects of HMP and GHMP against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in human erythrocytes. The inhibition rate of hemolysis, the reactive oxygen species (ROS) level, the concentration of malondialdehyde (MDA), the reduced glutathione (GSH) and oxidized glutathione (GSSH), and the enzymatic activities of total superoxide dismutase (SOD), catalase (CAT), and cellular glutathione peroxidase (GSH-Px) were evaluated as the biomarkers of oxidative status in human erythrocytes. The results showed that HMP and GHMP effectively inhibit the occurrence of erythrocyte hemolysis in the range of 0.025–1.0 mg/mL, and the inhibition rates of HMP and GHMP reached 92% and 90% at concentrations of 0.4 mg/mL and 1.0 mg/mL, respectively. HMP and GHMP reduced the AAPH-induced oxidative hemolysis damage via suppressing the generation of ROS by inhibiting the formation of MDA, maintaining the balance of GSH/GSSG, and preserving the activities of the antioxidant enzymes, including SOD, GSH-Px, and CAT. Our findings revealed that both HMP and GHMP could be used as natural antioxidants, and have the potential for further application in the development of functional foods.Keywords:mulberry leaf;digested protein hydrolysates;erythrocytes;oxidative stress;natural antioxidants
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yuyan Wei,Ling Xie,Bertrand Muhoza,Qian Liu,Shiqing Song
The present study investigated the impact of four chicken liver protein hydrolysate-based cat food attractants on palatability. Aroma compounds were analyzed in these attractants, which were subsequently sprayed onto four different types of cat foods. Results revealed that CF4 exhibited the highest intake ratio and the first choice ratio, followed by CF2 sample. Orthogonal partial least-squares discriminant analysis (OPLS-DA) demonstrated significant differences among 50 volatile compounds identified from the four cat foods. Using variable importance in projection (VIP) values, we selected 17 key flavor compounds responsible for distinguishing between the four cat foods. Peptides with a molecular mass <180 Da showed correlation with nonanoic acid and cedrol, while those >3000 Da correlated with hexanoic acid ethyl ester. Regression coefficients (RCs) calculated from partial least-squares regression (PLSR) results showed positive correlations between compound content and palatability for six compounds, whereas negative correlations were observed for ten compounds. Validation experiments confirmed that nonanal, 2-propylpyridine, and 3-octen-2-one enhanced palatability and correlated with peptides ranging from 180 to 500 Da; conversely, nonanoic acid ethyl ester and 3-methyl-pentanoic acid reduced palatability and correlated with peptides ranging from 1000 to 3000 Da.
研究领域:
食品科学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROENCAPSULATION
·
2019
·
IF 2.04
Bei Jin,Xiaosong Zhou,Shanshan Zhou,Yuan Liu,Zhiyuan Zheng,Yuxin Liang,Siting Chen
Purpose: To evaluate the feasibility of soy protein hydrolysates (SPH)-tannic acid (TA) complex nanoparticle obtained by photocatalysis (SPH-T (P)) to construct curcumin (Cur) delivery vehicles.Methods: The interaction behaviour of SPH-T (P) was investigated using Fourier transform infra-red, X-ray diffraction and differential scanning calorimeter analyzes. Formation and stability of the complexes were characterised by particle size, morphology, zeta potential, and in vitro release.Results: Negatively charged Cur-loaded complex with small size (<100 nm), spherical cluster shape and uniform size distribution were formed through the driving force of electrostatic attraction, followed by hydrogen bonding. The presence of photocatalysis in the complexes significantly improved the storage stability and in vitro sustained release of curcumin by enhancing the hydrogen bonding, hydrophobic effects and π–π stacking interactions between SPH and TA.Conclusion: SPH-T (P) would be a useful and promising delivery vehicle for encapsulating, protecting, and delivering hydrophobic nutraceuticals.
研究领域:
食品科学
·
药物递送
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 14
·2083
·
IF 5.561
Ye Dong,Wen Yan,Yi-Qi Zhang
The physicochemical, structural properties, antioxidant, and angiotensin I-converting enzyme (ACE) inhibitory activities of fish skin protein hydrolysate (SPH) that were freeze-dried (SPH-FD) and spray-dried (SPH-SD) were investigated. SPH-SD showed abundant volatile compounds, higher DPPH radical scavenging activity and ferrous iron chelating activity than SPH-FD, while the ABTS radical scavenging activity and ACE inhibitory activity were not influenced by the drying method. Amino acid compositions showed a higher proportion of proline and hydroxyproline residues in SPH-FD. The major molecular weights were both distributed below 1000 Da. SPH-SD had spherical structures, while SPH-FD had glass shard-like structures. The results indicated that the drying method could affect the physicochemical properties of hydrolysates, and SPH-SD showed potential prospects in developing functional fortified foods.Keywords:fish skin hydrolysate;freeze drying;spray drying;antioxidant;ACE inhibitory activity
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Smart Medicine
·
2024
·Vol. 3
·期 3
·e20240027
·
IF 0
Fanfan Fu,Changyi Liu,Zhenlin Jiang,Qingyu Zhao,Aining Shen,Yilun Wu,Wenyi Gu
Silk fibroin (SF)-based hydrogels are promising multifunctional adhesive candidates for real-world applications in tissue engineering, implantable bioelectronics, artificial muscles, and artificial skin. However, developing conductive SF-based hydrogels that are suitable for the micro-physiological environment and maintain their physical and chemical properties over long periods of use remains challenging. Herein, we developed an ion-conductive SF hydrogel composed of glycidyl methacrylate silk fibroin (SilMA) and bioionic liquid choline acylate (ChoA) polymer chains, together with the modification of acrylated thymine (ThyA) and adenine (AdeA) functional groups. The resulting polymeric ion-conductive SF composite hydrogel demonstrated high bioactivity, strong adhesion strength, good mechanical compliance, and stretchability. The formed hydrogel network of ChoA chains can coordinate with the ionic strength in the micro-physiological environment while maintaining the adaptive coefficient of expansion and stable mechanical properties. These features help to form a stable ion-conducting channel for the hydrogel. Additionally, the hydrogel network modified with AdeA and ThyA, can provide a strong adhesion to the surface of a variety of substrates, including wet tissue through abundant hydrogen bonding. The biocompatible and ionic conductive SF composite hydrogels can be easily prepared and incorporated into flexible skin or epidermal sensing devices. Therefore, our polymeric SF-based hydrogel has great potential and wide application to be an important component of many flexible electronic devices for personalized healthcare.
研究领域:
生物材料
·
组织工程
·
柔性电子器件
✓ 已验证
DOI
Food Bioscience
·
2023
·
IF 5.2
Kefan Ouyang,Qian Chen,Hexiang Xie,Qin Zhang,Liling Tao,Hua Xiong,Yulan Du,Jiangang Yan,Xinliang Mao,Qiang Zhao
Arthrospira cell residue (ACR) is a byproduct of the spirulina industry that can be valorized into protein hydrolysates. In this study, we used alkaline protease to hydrolyze ACR and evaluated the effects of hydrolysis time on the properties and functionalities of the hydrolysates. We measured the degree of hydrolysis (DH), particle size, fluorescence, UV‒visible absorption, surface hydrophobicity , emulsification , solubility, and antioxidant activity of the hydrolysates. The DH reached a maximum of 10.2% ± 0.6% after 240 min. Hydrolysis also altered the structure and conformation of the ACR protein, as shown by changes in fluorescence, UV-visible absorption, and FTIR spectra. Hydrolysis enhanced the solubility, surface hydrophobicity , and emulsifying activity of the hydrolysates but not the emulsion stability. The hydrolysates also exhibited higher antioxidant activity than ACR. These results indicate that enzymatic hydrolysis can convert ACR into valuable protein hydrolysates.
研究领域:
生物技术
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yaoqi Chen,Xu Cao,Jie Yao,Zeming Hu,Yang Luo,Gonghui Li,Hua Zhang,Kerong Wu
Hemorrhagic Cystitis (HC) presents a significant therapeutic challenge due to the dynamic fluid environment and cyclical mechanical stress within the bladder. Tissue-adhesive hydrogels have shown promise in treating HC; however, maintaining strong adhesion and mechanical integrity under these fluctuating conditions remains a critical obstacle. Herein, we designed a robust bladder-adhesive hydrogel by leveraging the affinity of tea polyphenols (TP) for damaged tissues and their ability to rapidly enhance the stability of photo-crosslinked silk fibroin methylacryloyl (SFMA) through abundant hydrogen bonding. The resulting SFMA/TP hydrogel could withstand high compressive and tensile loads while maintaining efficient under-urine adhesion, achieving up to 15.1 kPa to adapt to the dynamic mechanical environment of the bladder. Furthermore, urea dissociation disrupted hydrogen bonding, enabling the SFMA/TP hydrogels to exhibit urea-responsiveness and effective biodegradation both in vitro and in vivo within the bladder. In a rat model of cyclophosphamide-induced HC, this under-urine hydrogel adhesive demonstrated superior hemostatic effects and promoted healing by modulating inflammation, enhancing neovascularization, and facilitating smooth muscle formation. Overall, this bladder-adaptive hydrogel adhesive represents a minimally invasive therapeutic option for HC by offering targeted and sustained treatment within the bladder environment.
研究领域:
生物医学工程
·
材料科学
·
病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
KEY ENGINEERING MATERIALS
·
2016
·
IF 0
Mi Mi Chen,Meng Meng Zhang,Feng Qing Zhao
A clean and safe process of utilizing waste penicillin mycelium (WPM) was proposed in this paper. We treated WPM by soaking it in aqueous alkaline solution, to produce retarders for hemihydrate gypsum (the protein hydrolysate) by filtration. The filter cake was used to produce activated carbon, while the waste water can be reused for soaking process. This retarder can effectively prolong the setting time of gypsum with less drop in mechanical strength of gypsum plaster. The activated carbon produced with 674 mg/g of Iodine value, which meets the requirements of general industrial applications.
研究领域:
生物工程
·
材料科学
·
环境科学
✓ 已验证
DOI
JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 87
·期 1
·302-311
·
IF 3.167
Yi-Nan Du,Shan Xue,Jia-Nan Yan,Xin-Yu Jiang,Hai-Tao Wu
The objective of this study was to investigate the properties of ternary composite gel of scallop ( Patinopecten yessoensis ) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG). The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed. The results showed that the G ′ value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG. FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs. Moreover, the cryo-SEM results showed that SMGHs/KC/XG exhibited a tighter, smoother, and more aggregated microstructure than those of SMGHs, SMGHs/KC, and SMGHs/XG. These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications. Practical Application Scallop ( Patinopecten yessoensis ) male gonads are rich in protein and usually regarded as byproducts during adductor processing. Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food. The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields. Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.
研究领域:
食品科学
·
生物材料学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）