ELECTROCHEMISTRY
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2024
·Vol. 92
·期 4
·043015-043015
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IF 2.7
Yuxiang ZHONG,Yuanjia MA,Yangzhou HE,Xiao YANG
The authors focus on the development of a molten salt processing technology for extracting white phosphorus (P4) from sewage sludge ash, which is recognized as a potential secondary P source due to its composition of various metal phosphates. This work offers insights into the interaction between solid AlPO4 and molten CaCl2. The results show that solid AlPO4 exhibits favorable solubility in molten CaCl2, although at a slower rate compared to solid Ca3(PO4)2 due to the presence of stable Al–O bonds. The study also demonstrates the feasibility of extracting high-purity P4 from the molten mixture through electrolysis. The purity of the obtained electrolyzed P4 from AlPO4 is comparable to the commercially available high-purity-grade product. These findings underscore the potential of sewage sludge ash containing AlPO4 as a viable source for extracting high-purity P4. This research contributes to the advancement of sustainable P utilization technologies from secondary sources.
研究领域:
环境科学
·
化学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuoshuo Yang,Yuhua Huang,Yonggang Lv
Owning to seriously impaired capacity of bone regeneration, the repair of osteoporotic bone defect remains a major clinical challenge in orthopedics. For titanium mesh scaffolds of skull repair, to construct bio-coatings targeting the pathological environment of osteoporosis is significant. Here, tannic acid (TA)/casein phosphopeptide (CPP) based layer-by-layer (LBL) self-assembled coating that loaded with anti-osteoporotic alendronate sodium (AS) and bioactive Mg 2+ were prepared. The TA/CPP based LBL coatings showed good antioxidative function to effectively clear ABTS +• free radicals (scavenging rate of 64.29 ± 20.21 %) and inhibited the production of reactive oxygen species (ROS) in bone marrow mesenchymal stem cells (BMSCs) under oxidative stress conditions. All the LBL coatings exhibited good blood compatibility, and promoted early adhesion of BMSCs without affecting cell proliferation. In particular, the (TA/CPP-AS NPs) 4 + Mg 2+ coatings had both good alkaline phosphatase (ALP) activity and in vitro osteogenic mineralization, and could effectively promote the migration of human umbilical vein endothelial cells (HUVECs). In 8-weeks in vivo implantation experiments of osteoporotic skull defects, AS and Mg 2+ loaded LBL coating showed significant formation of new bone tissue. The study on the integrated system of antioxidative coating with bisphosphonates and active metal ions will serve as a promising strategy for osteoporotic bone defect repair.
研究领域:
生物材料学
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骨科医学
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再生医学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Licheng Cao,Yameng Zhao,Zhanying Chu,Xiangmin Zhang,Weibing Zhang
In proteomics, phosphorylation is an important process for protein post-translational modification (PTM), which greatly improves the diversity of proteomes. The PTM regulates almost all physiological and pathological processes such as signal transduction , cell division, proliferation, differentiation and metabolism. The abnormal expression of protein phosphorylation is also associated with cellular metabolic disorders and a range of diseases. However, in mass spectrometry-based phosphorylated peptideomics studies, phosphorylated peptide signals were inhibited by a high abundance of non-phosphorylated peptides; thus, highly selective enrichment was required. In this study, a newly designed material named Fe 3 O 4 @MIL(Fe/Ti) was synthesized using a layer-by-layer self-assembly technique that coats the surface of magnetic oxide nanospheres with bimetallic MOF of iron and titanium. The synergistic synthetic coating of the bimetallic MOF gives the material a large surface area and excellent hydrophilicity , which endow the nanoparticles with excellent phosphopeptide enrichment ability, high selectivity (β-casein/BSA molar ratio 1:500), a low detection limit (3 fmol), high recovery rate (85%), strong binding capacity, size exclusion ability, and ideal batch-to-batch repeatability. For comparison, we used Fe 3 O 4 @MIL(Fe/Ti) and two single-metal MOF materials Fe 3 O 4 @MIL-100(Fe) and Fe 3 O 4 @MIL-125(Ti), to enrich α-casein in the middle. Thus, the iron-titanium bimetallic MOF can not only enrich all the phosphorylated peptides enriched by Fe 3 O 4 @MIL-100(Fe) and Fe 3 O 4 @MIL-125(Ti), but can also specifically enrich four phosphorylated peptides. Encouraged by the excellent results of characterization and standard protein enrichment, we used this material to analyze human serum and found that bimetallic materials can effectively enrich all four phosphorylated peptides and exclude high molecular proteins. These experimental results indicate that the novel bimetallic MOF is a good candidate to analyze protein phosphorylation in complex samples.
研究领域:
蛋白质组学
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生物化学
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材料科学
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医学
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-19
·
IF 12.6
Wang Lirui,Xiong Manyi,Zhang Jian,Li Sisi,Ma Sheng,Jiang Shengyao,Jiang Yanping,Li Xinhong
Introduction Preservation of porcine semen is essential for artificial insemination and genetic improvement in pig breeding programs. However, the overproduction of reactive oxygen species (ROS) and lower levels of protein phosphorylation emerge as two challenges during semen preservation. Inspired by the innate ligand-receptor binding biofunction of dopamine, herein, a dual-task nano-protectant that combines ROS-scavenging and protein phosphorylation-regulating properties via incorporating the natural antioxidant epigallocatechin gallate (EGCG) into polydopamine nanoparticles (EGCG@PDA NPs) was proposed to enhance the quality of pig semen during storage at 4 ℃. The results suggested that EGCG@PDA NPs significantly maintained sperm motility, acrosome integrity and mitochondrial membrane potential, extending semen storage time from 3 days to 10 days. Furthermore, EGCG@PDA NPs effectively scavenged excess ROS and inhibited ROS-mediated sperm apoptosis through the extracellular regulated protein kinases (ERK) signaling pathway. Intriguingly, EGCG@PDA NPs could degrade into ultrasmall particles (< 10 nm) in the semen or H 2 O 2 systems. These particles could target and activate the dopamine D2 receptor (D2DR) on membrane surface of sperm midpiece, thereby enhancing protein phosphorylation via the downstream cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) signaling pathway, ultimately improving sperm motility parameters. This study presents a novel nano-strategy to boost the quality of pig semen, offering significant implications for the pig industry. Graphical
研究领域:
动物繁殖学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jie Zhou,Xianxin Qiu,Kaiqi Su,Gaixia Xu,Ping Wang
Based on the inhibition of okadaic acid (OA) on protein phosphatase 2A (PP2A), we developed disposable poly- o -aminophenol-carbon nanotubes (PoAP-CNTs) modified screen print electrode (SPE)-based electrochemical enzyme sensor for OA detection. Electropolymerized PoAP-CNTs composite film was coated on electrode for the immobilization of PP2A. Owing to the excellent physicochemical characteristic of PoAP-CNTs film, the performance of enzyme sensor was enhanced. Under the optimal conditions, the biosensor provides a larger linear range, and lower detection limit for the analysis of OA compared with colorimetric PP2A inhibition assay and PP2A/SPE. The spiked OA shellfish samples were applied to verify the efficiency of method for OA detection. The results shows acceptable recovery rate and low RSD, demonstrating the applicability of this method for in suit OA assessment in the quality and toxicity monitoring of seafood.
研究领域:
生物化学
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毒理学
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分析化学
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DOI
高影响因子
CHROMATOGRAPHIA
·
2022
·Vol. 86
·期 1
·97-105
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IF 2.213
Wang Baichun,Du Jianglong,Liu Bin,Xu Qian,Huang Jingli,Liu Chunbo,Yan Yinghua,Ding Chuan-Fan
Nasopharyngeal carcinoma (NPC), as a malignant tumor, has increasingly affected the health and survival of human, and certain changes in the phosphorylation level of protein have been found in the serum of NPC patients. Thus, the investigation of phosphoproteins/phosphopeptides in human serum of NPC patient will conduce the understanding of the related pathogenesis and pathological processes. However, there are certain limitations in the direct detection of phosphoprotein/phosphopeptide in patient serum by mass spectrometry. Here, we designed a simple and green synthetic route to prepare hollow TiO 2 spheres for phosphopeptide pre-treatment using the hard template method with carbonaceous spheres as the template, providing a novel path for the exploration of hollow spheres and even functionalized probes. The TiO 2 spheres with unique hollow structure exhibit a low detection limit (0.2 fmol), high loading capacity (250 mg/g), stable reusability (ten cycles), high selectivity (1:1500), and relatively good recovery rate (80.3%). Besides, hollow TiO 2 spheres identify 25 phosphopeptides with 19 phosphorylation sites corresponding to 18 phosphoproteins and 15 phosphopeptides with 14 phosphorylation sites corresponding to 10 phosphoproteins from the serum of the healthy person and NPC patient, respectively. Among them, four phosphopeptides with five phosphorylation sites are only detected in NPC samples. After gene ontology analysis of cell components and molecular functions, it is discovered that the identified phosphoproteins are inseparable from NPC related events, including heparin binding, lipid binding, endoplasmic reticulum cavity, and early endosomes, signaling that related functional pathways may be involved in the pathological process of NPC in some way.
研究领域:
生物化学
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肿瘤学
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医学诊断
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Xiao Fei,Shi Jingjing,Zhang Xinhai,Hu Min,Chen Kangming,Shen Chao,Chen Xiaodong,Guo Yaping,Li Yang
Reducing the incidence of bone defects caused by trauma and other primary diseases is an urgent task in modern society. In the present study, we developed a gadolinium-doped whitlockite/chitosan (Gd-WH/CS) scaffold and assessed its biocompatibility, osteoinductivity, and bone regeneration capacity for the treatment of calvarial defect in a Sprague-Dawley (SD) rat model. The Gd-WH/CS scaffolds possessed a macroporous structure, with a pore size ranging 200–300 μm, which facilitated the growth of bone precursor cells and tissues into scaffold. Results of cytological and histological biosafety experiments showed that both WH/CS and Gd-WH/CS scaffolds were non-cytotoxic to human adipose-derived stromal cells (hADSCs) and bone tissue, which demonstrated the excellent biocompatibility of Gd-WH/CS scaffolds. Results of western blotting and real-time PCR analysis provided a possible mechanism that Gd3+ ions in the Gd-WH/CS scaffolds promoted the osteogenic differentiation of hADSCs through the GSK3β/β-catenin signaling pathway and significantly upregulated the expression of osteogenic related genes (OCN, OSX and COL1A1). Finally, in animal experiments, SD rat cranial defects were effectively treated and repaired with Gd-WH/CS scaffolds due to its appropriate degradation rate and excellent osteogenic activity. This study suggests the potential utility of the Gd-WH/CS composite scaffolds in treating bone defect disease.
研究领域:
生物材料学
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骨组织工程
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医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
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2025
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IF 7.9
Jiangping Song,Mengjun Wu,Tian Tian,Sixiu Zeng,Dan Liu,Hao Li,Shengqiu Zhao,Haolin Tang
The vaporization-condensation-conversion method is the prominent technique used for synthesizing red phosphorus/carbon (P/C) composites. However, its practical application is limited by the inevitable formation of toxic white phosphorus (WP) residues, posing severe safety concerns. Theoretical calculations of the WP polymerization process performed in this study revealed that the introduction of iodine can considerably accelerate the polymerization of WP molecules, yielding P/C composites free of WP residues. Furthermore, in the resulting iodine-doped P/C (I-P/C) composites, iodine forms I-P bonds, considerably enhancing electronic conductivity and promoting faster Na-P redox kinetics compared to those in the original P/C composites. Consequently, an I-P/C electrode exhibited a capacity of 1193 mA h g⁻ 1 , retaining 100 % of its capacity after 100 cycles at 0.2 A g⁻ 1 . Furthermore, an assembled I-P/C||Na 3 V 2 (PO 4 ) 2 F 3 full cell retained 91.9 % of its capacity after 200 cycles at 0.5 A g⁻ 1 . Additionally, the full cell exhibited a high energy density of 132.2 W h kg⁻ 1 at a power density of 9942.1 W kg⁻ 1 , demonstrating potential to complete charging or discharging within ∼57 s. Finally, an I-P/C||Na 3 V 2 (PO 4 ) 2 F 3 pouch cell, with a capacity of 0.36 A h, demonstrated excellent cycling performance even after 150 cycles at 0.05 A g⁻ 1 .
研究领域:
材料科学
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能源化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Zhihong Guan,Ruobo Chen,Kaixin Liu,Li Fu,Ling Li,Li Jia
Developing an on-site sensing platform for casein phosphopeptide (CPP) is essential to ensure the quality of infant formula. This study is the first report on a fluorescence detection method for CPP. The method is based on the readily available copper ions (Cu 2+ ). Cu 2+ has peroxidase-like activity and can catalyze the oxidation of o -phenylenediamine (OPD) to produce 2,3-diaminophenazine with a fluorescence property. CPP can inhibit the peroxidase activity of Cu 2+ through the coordination of the phosphate groups in CPP and suppress the oxidation of OPD, decreasing the fluorescence intensity of the catalytic system. Based on this principle, a fluorescence approach was developed to detect CPP in milk and milk powder samples. The method does not require the preparation of nanomaterials, and the detection process takes 25 min. Furthermore, a self-designed portable detection device was constructed to detect CPP, demonstrating the potential of the fluorescence sensing platform in the on-site detection of CPP.
研究领域:
食品科学
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
ACS Chemical Biology
·
2025
·
IF 3.8
Jinying Gu,Chenxi He,Zeyu Han,Qifei Huang,Yanyi He,Yun Lu,Qidong You,Qiuyue Zhang,Lei Wang
Hyperphosphorylation of proteins is implicated in various diseases, such as phosphorylated Tau (p-Tau), which is the main cause of Alzheimer’s disease (AD). Dephosphorylation strategies have still been limited. Currently, phosphatase recruitment chimeras (PHORCs) have become a potential strategy for accelerating the dephosphorylation of proteins. However, PHORCs are still in the proof-of-concept stage. The paucity of available phosphatase effectors and the lack of effective methods to identify the appropriate length of the linker impede the development of PHORCs. Protein phosphatase 5 (PP5) is responsible for dephosphorylation of p-Tau in the brain. PP5 is distinct from other phosphatases, with a unique activation mechanism. We demonstrated that PP5 can be simultaneously recruited and activated for the design of PHORCs, exhibiting a synergistic advantage for accelerating dephosphorylation of p-Tau. Moreover, we attempted computation-aided prediction methods to obtain the potential length of the linker, promoting the rational design of PHORCs. Therefore, our study provides critical insights into the development of PHORCs and proposes new ideas for accelerating the design of heterotrimeric chimeras.
研究领域:
生物化学
·
神经科学
·
药物开发
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yiqiao Pei,Ye Zhang,Fang Tian,Yanrong Zhao,Xuguang Zhang,Steve W. Cui,Huali Wang,Jianbo Zhang,Hao Wang
Iron supplementation has been an important and complicated health topic. Available iron preparations generally have issues such as instability, inadequate absorption, and low consumer acceptability. This study provided a novel ferric pyrophosphate (FePP) delivery system, the FePP-casein-phosphopeptide-dextran (FCD) complex, with sodium pyrophosphate (NaPP) as a solvent promoter and casein-phosphopeptide (CPP) and β-glucan as wall materials. Compared to FePP, FCD showed better properties, including good water solubility (95% resolubilization), color stability (pH 3.5–6.5), compatibility with sensitive nutrients like vitamin C (VC), and lipid oxidation stability (delayed by approximately 50%). In vitro, FCD demonstrated gradual iron release in the gut with a release rate exceeding 80%. In vivo, FCD effectively alleviated iron deficiency anemia (IDA) symptoms in mice, with superior overall efficacy to FePP. Therefore, this novel FCD iron delivery system had considerable potential to be applied in nutritional food systems or iron supplements.
研究领域:
营养学
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食品科学
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生物医学工程
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药剂学
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DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2251-2260
·
IF 3.289
Guo Liu,Shengwei Sun,Baoyan Guo,Benchun Miao,Zhen Luo,Zumeng Xia,Danyang Ying,Fei Liu,Bin Guo,Jian Tang,Yong Cao,Jianyin Miao
Casein phosphopeptides (CPPs) have been demonstrated to be calcium chelators. Unfortunately, few studies have been reported on the effects of CPPs on the mechanism of the uptake and absorption of Ca2+ and bone metabolism. In this study, a monomeric peptide fraction isolated by RP-HPLC (F6-1) that possessed high calcium transport capacity in Caco-2 cell monolayers was separated and characterized. The effects of F6-1 on the absorption mechanisms of Ca2+ in a Caco-2 monolayer model and bone metabolism in rats were investigated. F6-1 was isolated by preparative and analytical RP-HPLC. Results for calcium transport suggested that the rates of Ca2+ transportation by F6-1 were approximately 2.57, 2.87 and 2.38 times higher than those in the control group at 30, 60 and 120 min, respectively. Results of ultraviolet (UV) spectroscopy indicated that the intensity of UV absorption changed because of the binding of Ca2+ to F6-1. Analysis of transepithelial electrical resistance (TEER) and the expression of TRPV6 in Caco-2 cells showed that F6-1 was likely to influence the transcellular pathway of intestinal absorption of Ca2+ rather than the paracellular pathway. Furthermore, the F6-1 group (1% Ca, 0.03% F6-1) exhibited increases in serum Ca2+ levels, femur length and femur Ca and decreases in serum alkaline phosphatase (ALP) levels and urinary pyridinoline content in a Sprague-Dawley rat model, which implied that F6-1 was beneficial for bone calcification. Overall, our results suggested that F6-1 enhanced the transport of Ca2+ in Caco-2 cells by affecting the transcellular pathway by upregulating the expression of TRPV6. F6-1 also improved bone formation and prevented bone resorption to benefit bone health in rats, which provided a basis for using F6-1 in calcium supplements or functional foods.
研究领域:
营养学
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生物化学
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食品科学
·
生理学
✓ 已验证
DOI
PubMed
高影响因子
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