BIOCHEMICAL ENGINEERING JOURNAL
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2021
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IF 3.978
Jinqiong Wang,Yi Xie,Jie Hou,Xinyu Zhou,Jing Chen,Changhong Yao,Yongkui Zhang,Yonghong Li
The wide application of biodegradation technology makes it particularly significant to use immobilized microorganisms to achieve continuous degradation of pollutants. In this study, the performance of Ca-alginate (CA) immobilized Phanerochaete chrysosporium beads in a consecutive biocycle treatment of bisphenol A (BPA) effluent was researched. The effects of initial BPA concentration, biomass dosage, pH and curing time were investigated. Results showed that CA-immobilized P. chrysosporium can be successive cycle at least 9 times (12 h each), and each time the removal efficiency of BPA reached 100%. The degradation process of BPA in the treatment process was investigated. According to the analyses of glucose, ammonia nitrogen, total nitrogen, extracellular proteins and biomass, it was found that consumed carbon and nitrogen sources were used for the rapid growth of fungal hyphae and the synthesis of a series of proteins. These extracellular proteins can be utilized as a nitrogen source by P. chrysosporium , and played a key role in the removal of BPA. Biodegradation process followed the pseudo-first-order kinetics. Moreover, intermediates in the BPA degradation process were identified by gas chromatography-mass spectrometry (GC–MS) and liquid chromatography-mass spectrometry (LC-MS), and three possible degradation pathways were proposed. This strategy presented in the study provides a convenient and efficient biological method for the continuous treatment of BPA effluent.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Yaping Ma,Minchang Liu,Ruifang Gu,Rongya Zhang,Xiaomei Ji,Juan Zhang,Wu Wen,Zheng Peng
Introduction: Laccase exhibits significant applications in food additives, wastewater treatment, and biomass processing. Fungal laccase exhibits high activity, good stability, and excellent performance. However, scale-up production of high-yield laccase from fungi is challenging. This study aimed to identify crucial factors affecting enzyme production and analyze the enzymatic properties of laccase during fermentation.Methods: A laccase-producing white-rot fungus was used for fermentation process optimization in 200 L and 1200 L fermenters. The Plackett–Burman design revealed three significant influencing factors: temperature, aeration ratio, and agitation speed. The steepest ascent experiment was used to approximate the maximum response region, followed by the establishment of a regression model between experimental factors and laccase activity using the Box–Behnken response surface methodology and optimal fermentation condition selection.Results: The optimal conditions for laccase production by Ganoderma lucidum fermentation were 30°C temperature, 0.66 aeration ratio, and 100 rpm agitation speed, achieving a maximum laccase activity of 214,185.2 U/L. Dissolved oxygen (DO) was a crucial factor for high laccase yield, and its maintenance at a high level in the fermentation system significantly enhanced the enzyme activity. Fermentation batches with varying laccase production levels exhibited a trend of decreasing pH and a subsequent increase in the mid-to-late fermentation stages. With decreased pH, the DO level started declining; when DO stabilized, the pH started to rebound, coinciding with the peak laccase activity, indicating a signal of fermentation endpoint in industrial production.Discussion: This study provides valuable theoretical and data support for the industrial production of laccase by fungi through optimized fermentation processes.
研究领域:
生物技术
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微生物学
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生化工程
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2020
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IF 4.074
Li Yan,Feiyang Zeng,Zhangjing Chen,Shuang Chen,Yafang Lei
Wood is an environmentally friendly product , but some of woods can be easily biodegraded. Salicylic acid/silica microcapsules (SSM) represents a kind of wood preservative prepared with the anti-fungal salicylic acid as a core substance and the silica as wall material using sol-gel technology . SSMs were synthesized and then impregnated into poplar wood using vacuum-pressure treatment. Leaching tests were carried out in distilled water for up to 318 h. Decay resistance of SSM treated poplar against white-rot fungi ( Trametes versicolor ) and brown rot fungi ( Gloeophyllum trabeum ) was determined. Leaching tests showed that SSM retention was good and leaching resistance of salicylic acid in SSM treated wood were improved. SEM (scanning electron microscope), FTIR (Fourier-transform infrared spectroscopy), EDX (Energy Dispersive X-Ray Spectroscopy) and XPS (X-ray photoelectron spectroscopy) analysis showed that the SSMs were between 0.06 and 0.16 μm, and most were distributed in the vessel cavity with hydrogen bonding to cell wall surfaces, some SSMs were found in the cell wall. The decay resistance of SSM treated poplar was greatly improved compared to untreated poplar.
研究领域:
林学
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材料科学
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微生物学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Zhenju Bi,Jeffrey J. Morrell,Yafang Lei,Li Yan,Meitong Ji
Wood protection using plant-derived extracts has shown some promise but the extracts are often easily leached making them less attractive for long-term wood protection. The potential of extracts from heat treated wood sawdust as a natural substitute for wood preservatives was explored, and a mild enzyme-catalyzed method was used to improve its leaching resistance. Laccase was used to catalyze grafting reactions of extracts from heat treated spruce ( Picea asperata Mast.) and larch ( Larix gmelinii (Rupr.) Kuzen.). The resulting extracts were screened for antifungal activity and then impregnated into poplar blocks ( Populus tomentosa Carr.). Fourier Transform Infrared Spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to characterize extracts reactions. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the grafted compounds. Acetone extracts from both heat treated spruce and larch exhibited good activity against the test fungi. Heat treated spruce and larch acetone extracts grafted on modified poplar and subjected to leaching showed better activity against the white-rot fungus, Trametes versicolor (L. ex Fr.) Quél. than the brown-rot fungus, Gloeophyllum trabeum (Pers.: Fr.) Murr. FTIR and XPS results indicated that heat-treated spruce and larch extracts were grafted on poplar by laccase catalysis. Squalene, 3-hydroxy-4-methoxybenzoic acid, and n-hexadecanoic acid were among those compounds suggested to be grafted. The results suggest that laccase-mediated pre-treatment of plant extracts has the potential to increase resistance to leaching. Trials with additional plant-extracts are recommended.
研究领域:
林学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）