JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Mingwei Zhao,Shijin Xiong,Tonghao Du,Xiaoyan Xu,Huiyu Li,Linli Zhang,Yazhou Xu,Tao Wei,Hongbing Ren,Tao Xiong,Mingyong Xie
Background Chinese spicy cabbage (CSC) is a famous traditional fermented vegetable widely consumed in northeast China. However, the differences in characteristics between Liaoning spicy cabbage (LNSC) and Yanbian spicy cabbage (YBSC), as well as the correlation between flavor attributes and microbiota remain unclear. This study clearly delineated the characteristics and correlations of ingredients, aroma characteristics and microbial communities between LNSC and YBSC. Results Metabolomic analysis revealed distinct compositional differences in both volatile and non-volatile metabolites between LNSC and YBSC. Through relative odor activity value analysis, 17 and 14 key flavor compounds were identified as characteristic components in LNSC and YBSC, respectively. Amplicon sequencing demonstrated significant regional variations in the bacterial community structure of CSC. Spearman correlation analysis demonstrated strong correlations between 20 bacteria and 11 free amino acids, 22 volatile flavor compounds. Conclusion This study systematically compared the quality characteristics between LNSC and YBSC, providing fundamental data for the evaluation of spicy cabbage and fermented food products. © 2025 Society of Chemical Industry.
研究领域:
食品科学
·
微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Food Research
·
2025
·
IF 6.2
Ai Liu,Fengyu Li,Jiacheng Ge,Guodong Si,Xianzheng Han,Yun Gao,Wenli Wang,Li Su,Nan Zou,Wei Mu,Xiaomei Li,Xiuyu Pang
This study focuses on the development and validation of a detection method for indoxacarb residues in three major vegetable crops, Chinese cabbage, cabbage, and tomato, which are widely cultivated worldwide. The method was rigorously validated through field trials and dietary risk assessment. A strong linear response was observed for indoxacarb at the concentrations of 0.0005 - 0.25 mg/L, with a limit of quantitation of 0.01 mg/kg. The half-life of indoxacarb ranged from 4.61 to 6.89 days in Chinese cabbage, 3.06 to 6.21 days in cabbage, and 2.28 to 3.46 days in tomato. The terminal residues were <0.01 - 0.2 mg/kg in Chinese cabbage, <0.01 - 0.15 mg/kg in cabbage, and <0.01 - 0.029 mg/kg in tomato. Dietary risk assessment revealed a low probability of long-term risk (12.9 %) associated with the consumption of these crops. Overall, the study suggests that the use of indoxacarb on these three vegetables is safe for the general population, with minimal risk even under long-term exposure.
研究领域:
农学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 14
·2195
·
IF 4.1
Shan Chen,Yang Zheng,Qing Wang,Rong Mu,Xianchao Sun,Guanhua Ma,Liezhao Liu,Jiequn Ren,Kuo Huang,Guokang Chen
Clubroot, caused byPlasmodiophora brassicaeWoronin, poses a major threat to Chinese cabbage (Brassica rapasubsp.pekinensis) production worldwide, significantly impacting crop yield, quality, and economic value. Biological control represents a promising approach since it is non-toxic and eco-friendly, and it reduces the risk of pathogen resistance development. In this study, our objective was to screen for actinomycetes that can effectively inhibit clubroot. We screened 13 actinomycete strains, identifying 2, XDS3-6 and CD1-1, with substantial in vivo inhibitory effects, achieving infection suppression rates above 64% againstP. brassicae. Phylogenetic analysis classified XDS3-6 and CD1-1 asStreptomyces virginiaeandStreptomyces cinnamonensis, respectively. Both strains exhibited protease and glucanase production capabilities, essential for pathogenic suppression. Additionally, these strains induced host defense responses, as evidenced by increased jasmonic acid (JA) and salicylic acid (SA) accumulation and elevated activities of defense-related enzymes. Colonization studies of XDS3-6 and CD1-1 mutant strains in cabbage roots indicated sustained root colonization, with peak colony-forming units (CFUs) at 20 days post-inoculation, reaching 11.0 × 104CFU/g and 8.5 × 104CFU/g, respectively, and persisting for at least 30 days. Overall, these findings underscore the potential ofStreptomycesstrains XDS3-6 and CD1-1 as effective biocontrol agents, providing a theoretical foundation for their application in managing clubroot in Chinese cabbage.
研究领域:
植物病理学
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微生物学
·
农学
·
生物防治
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植物病害学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Wenkui Guo,Meiru Cheng,Xuemei Dong,Chuan Liu,Yu Miao,Peng Du,Hong Chu,Chun Li,Libo Liu
This study employed a combination of principal component analysis (PCA) and gas chromatography-ion mobility spectrometry (GC-IMS) to examine the distinctive taste mixtures produced by Chinese spicy cabbage (CSC) fermented at varying temperatures. As the fermentation progressed, the pH gradually decreased and stabilized after the 11 days of fermentation, and the total content of organic acids and short-chain fatty acids increased. A total of 49 volatile mixtures were detected during CSC fermentation and storage for 21 days. These included 7 aldehydes, 6 alcohols, 7 esters, 6 ketones, 5 pyrazines, 4 sulfides, 4 phenols, 2 ethers, 2 olefins, and 1 acid. With time, the content of most volatile flavor substances decreased. PCA of the signal intensities of the volatile chemicals in the samples showed significant differences in the flavor of CSC fermented at different temperatures; consequently, the samples fermented at different temperatures were effectively separated in relatively independent regions of CSC. Therefore, low-temperature fermentation and storage at 4 °C were more suitable for CSC. Based on the identified volatile chemicals, HS-GC-IMS and PCA could effectively construct the flavour fingerprints of CSC samples. This study provided a theoretical basis for improving the fermentation quality of CSC.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 20
·11026
·
IF 4.9
Han Zheng,Chao Yuan,Tong Bu,Qun Liu,Jingjuan Li,Fengde Wang,Yihui Zhang,Lilong He,Jianwei Gao
Identifying key genes involved in Cadmium (Cd) response pathways in plants and developing low-Cd-accumulating cultivars may be the most effective and eco-friendly strategy to tackle the problem of Cd pollution in crops. In our previous study,Stressseventy subfamily A 4(SSA4) was identified to be associated with Cd tolerance in yeast. Here, we investigated the mechanism ofSSA4in regulating Cd tolerance in yeast. ScSSA4 binds to POre Membrane 34 (POM34), a key component of nuclear pore complex (NPC), and translocates from the cytoplasm to the nucleus, where it regulates the expression of its downstream gene,Viable in a Hal3 Sit4 background 1(VHS1), resulting in reduced Cd accumulation in yeast cells. Additionally, we identified a Chinese cabbageSSA4gene,BrSSA4c, which could enhance the Cd tolerance in Chinese cabbage. This study offers new insights into the regulatory mechanisms of Cd tolerance in yeast, a model organism, and paves the way for the genetic enhancement of Cd tolerance in Chinese cabbage.
研究领域:
植物生物学
·
分子遗传学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 11
·4685-4696
·
IF 4.125
Lihui Zhang,Min Zhang,Arun S. Mujumdar,Kun Liu
BACKGROUND In order to prolong the storage and inhibit microorganisms of pakchoi, the antibacterial activity and mechanism of ultrasound combined with sodium hypochlorite (NaClO-US), the efficiency of NaClO-US in reducing Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa as well as preserving quality of pakchoi were investigated. RESULTS Ultrasound treatment could significantly reduce the usage of NaClO solution from 800 ppm to 500 ppm. NaClO-US decreased the counts of E. coli , S. aureus and P. aeruginosa , which disrupted the bacterial cell membrane with cytoplasmic leakage. In addition, NaClO-US significantly increased cell membrane permeability, while cell membrane integrity decreased, the secondary structure of bacterial proteins showed several obvious changes, such as the increase of random coil content, as well as the decrease of α -helix content. The bacterial counts, E. coli , S. aureus and P. aeruginosa population in pakchoi treated with NaClO-US reduced by 1.89, 1.40, 1.60, 1.72 log CFU g −1 , respectively compared to control sample after storage for 15 days. NaClO-US resulted in minimum chlorophyll depletion, flavor and sensory deterioration. CONCLUSION NaClO-US solution treatment inhibited microorganisms and prolonged storage of pakchoi. © 2022 Society of Chemical Industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2022
·
IF 4.342
Jiahua Ma,Yahya Faqir,Yunlong Chai,Sanmei Wu,Tong Luo,Shiyu Liao,Abdul Rasheed Kaleri,Chengjia Tan,Yuxin Qing,Mohammad Talib Kalhoro,Niaz Umer,Wazir Hadir
Agriculture lands have low fertilizer retention owing to porosity and irrigation, whereas a significant portion of fertilizer is lost through denitrification, volatilization, surface run-off, and leaching, causing losses of nutrients to air and ground/surface water. Urea fertilizer readily enhances the agricultural yield but also deteriorates the quality of fertile soils. To combat such problems, researchers are seeking to formulate controlled-release or slow-release fertilizers. We used chitosan microspheres as a carrier to encapsulate urea through emulsification and cross-linking techniques to reduce the wastage of urea fertilizer due to evaporation and water flow. Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy were used to characterize chitosan microspheres and urea-loaded microspheres. Chitosan microspheres and urea-loaded microspheres were authenticated by FT-IR, showing 89% of urea successfully incorporated with chitosan microspheres. Then an experiment was run to compare the effects on plant growth, antioxidant and metabolite contents with and without added nitrogen fertilizer. Various amounts of urea per pot were applied to Chinese cabbage, including T1 0.5433 g, T2 0.6033 g, T3 0.8033 g, CK 0.00, while the same content of urea in chitosan microspheres as T4, T5, T6, and T0 (no urea), respectively. The results indicated that chitosan microspheres-based controlled release nitrogen fertilizers (Cm-CRNFs) had higher seed germination (96%), plant height (17.04 cm), number of leaves (11.00), and leaf length (110.19 mm) as compared to urea and control treatments. In addition, Cm-CRNFs treatment enhanced net photosynthetic rate, stomatal conductance, transpiration rate, fluorescence, dark respiration rate, total sugar, proteins, flavonoids, and polyphenols. Overall, Cm-CRNFs showed promising results in all treatments for all tested physiochemical and growth parameters. It is concluded that controlled release urea fertilizers based on chitosan microspheres can be developed with a simple process. The fertilizers are biocompatible, economically viable, and environment-friendly, and have the potential to prolong the release of macronutrients and micronutrients for plant growth.
研究领域:
农业科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-13
·
IF 4.259
Gao Li-wei,Lyu Shan-wu,Tang Jun,Zhou Dao-yun,Bonnema Guusje,Xiao Dong,Hou Xi-lin,Zhang Chang-wei
Auxin resistant 1/like aux1 ( AUX / LAX ), pin-formed ( PIN ) and ATP binding cassette subfamily B ( ABCB / MDR / PGP ) are three families of auxin transport genes. The development-related functions of the influx and efflux carriers have been well studied and characterized in model plants. However, there is scant information regarding the functions of auxin genes in Chinese cabbage and the responses of exogenous polar auxin transport inhibitors (PATIs). We conducted a whole-genome annotation and a bioinformatics analysis of BrAUX / LAX, BrPIN , and BrPGP genes in Chinese cabbage. By analyzing the expression patterns at several developmental stages in the formation of heading leaves, we found that most auxin-associate genes were expressed throughout the entire process of leafy head formation, suggesting that these genes played important roles in the development of heads. UPLC was used to detect the distinct and uneven distribution of auxin in various segments of the leafy head and in response to PATI treatment, indicated that the formation of the leafy head depends on polar auxin transport and the uneven distribution of auxin in leaves. This study provides new insight into auxin polar transporters and the possible roles of the BrLAX, BrPIN and BrPGP genes in leafy head formation in Chinese cabbage.
研究领域:
植物学
·
分子生物学
·
发育生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 5
·1105
·
IF 5.2
Hongdou Gao,Shixian Zeng,Xiaozhen Yue,Shuzhi Yuan,Jinhua Zuo,Qing Wang
The effect of palmitic acid (PA) on stem browning was investigated in freshly harvested mini-Chinese cabbage (Brassica pekinensis). Results indicated that concentrations of PA ranging from 0.03 g L−1to 0.05 g L−1inhibited stem browning and decreased the rate of respiration, electrolyte leakage, and weight loss, as well as the level of malondialdehyde (MDA) in freshly harvested mini-Chinese cabbage stored at 25 °C for 5 d. The PA treatment enhanced the activity of antioxidant enzymes (ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), 4-coumarate:CoA ligase (4CL) and phenylalamine ammonia lyase (PAL)), and inhibited the activity of polyphenol oxidase (PPO). The PA treatment also increased the level of several phenolics (chlorogenic acid, gallic acid, catechin, p-coumaric acid, ferulic acid, p-hydroxybenzoic acid, and cinnamic acid) and flavonoids (quercetin, luteolin, kaempferol, and isorhamnetin). In summary, results indicate that treatment of mini-Chinese cabbage with PA represents an effective method for delaying stem browning and maintaining the physiological quality of freshly harvested mini-Chinese cabbage due to the ability of PA to enhance antioxidant enzyme activity and the level of phenolics and flavonoids during 5 d.Keywords:mini-Chinese cabbage;palmitic acid;stem browning;antioxidant enzyme;phenolics;flavonoids
研究领域:
植物科学
·
食品科学
·
采后生理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Enguang Nie,Haiyan Wang,Yan Chen,Yuhui Lu,Kashif Akhtar,Muhammad Riaz,Sufen Zhang,Zhiyang Yu,Qingfu Ye
Triclosan is a widely used biocide against microorganisms and is ubiquitously distributed in the environment. Triclosan can be accumulated into plants from soil and hydroponic media. However, little information is currently available on the comparative fate of triclosan in plants under soil and hydroponics cultivation conditions and factors governing uptake. Therefore, this study was designed to comparatively elucidate the uptake mechanism of 14 C-triclosan in youdonger ( Brassica campestris subsp. Chinensis var. communis ) grown under different soils and hydroponics and clarify dominant uptake factors. Results showed that 77.2% of 14 C were accumulated in youdonger grown in a hydroponic system, while only 1.24%–2.33% were accumulated in the two soil-planting systems. In addition, the bioconcentration factor (BCF) of 14 C-triclosan in soil-plant systems was approximately 400-fold smaller than that in the hydroponics. In the soil-planting system, a strong linear correlation was found between concentrations of triclosan in soil pore water and youdonger plant (R 2 > 0.85, p < 0.01) at different incubation times. Therefore, triclosan in pore water might be a good indicator to estimate its accumulation in plants and is significantly affected by soil pH, clay, and organic matter contents. The estimated average dietary intakes of triclosan for youdonger grown in hydroponic and soil-planting systems were estimated to be 1.31 ng day −1 kg −1 and 0.05–0.12 ng day −1 kg −1 , respectively, much lower than the acceptable dietary intakes of triclosan (83 μg day −1 kg −1 ), indicating no significant human health risks from youdonger consumption. This study provided insights into uptake routes of triclosan into youdonger plants from both soil and hydroponic systems, bioavailability of triclosan in different soils, and further assessment of human exposure to triclosan from youdonger.
研究领域:
环境科学
·
植物学
✓ 已验证
DOI
高影响因子
Food Science and Human Wellness
·
2020
·
IF 2.455
Chengwei Yu,Xu Huang,Yawei Fan,Zeyuan Deng
Pyrethroid is widely used in developing countries and is a potential threat to human health. So putting forward an effective pyrethroid cleaning method is of vital importance. Clearance of fenpropathrin, cypermethrin and deltamethrin in cabbage by using ultrasonic treatment accompanied with different baking soda concentrations were investigated in this study. The results of response surface methodology showed that the maximum clearance rate was 70.61% for fenpropathrin under the optimal power, treated time, treated temperature and baking soda concentration, which were 260.02 W, 10 min, 20.15 °C and 0.014 g/mL, respectively. Similarly, the maximum removal efficiency was 73.72% for cypermethrin under 240 W, 13.84 min, 19.53 °C and 0.014 g/mL, and 92.15% for deltamethrin under 238.99 W, 12.42 min, 25 °C and 0.013 g/mL. The clearance rate of pyrethroid by this method was significant higher ( P < 0.05) than traditional cleaning methods (bathing and running water washing). The contents of the compositions such as vitamin C, total soluble sugar, protein and dietary fiber, texture and sensory evaluation of cabbage were not found obvious changes after the ultrasonic treatment combined with baking soda ( P > 0.05). Results showed that ultrasonic treatment combined with baking soda was an effective way to eliminate the fenpropathrin, cypermethrin and deltamethrin in cabbage without influencing cabbage quality.
研究领域:
食品安全
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 18
·9887-9892
·
IF 3.049
MingNa Sun,Zhou Tong,Xu Dong,Yue Chu,Mei Wang,TongChun Gao,JinSheng Duan
Penflufen is a highly efficient, broad-spectrum succinate dehydrogenase inhibitor. Owing to the increasing pesticide resistance in recent years, the use of a new fungicide, penflufen, has become increasingly widespread. However, residues that remain in the environment after the use of penflufen have an impact on human health. It is worth noting that penflufen is a chiral pesticide. The differences of residue behaviors between two enantiomers in living organisms need to be systematically studied. In this paper, reversed-phase liquid chromatography-mass spectrometry (LC-MS) was used to separate the enantiomers of penflufen, and the absolute configuration of the enantiomer was analyzed. The LC-MS/MS methods for the analysis of penflufen enantiomers on wheat plants, spinach, and Chinese cabbage were established. The results of the recovery experiments showed that the average recovery of the two enantiomers was 78.5–99.8% and RSD was 0.4–7.3%, suggesting that the accuracy and precision of the method meet the requirements of pesticide residue analysis. The results of stereoselective degradation of penflufen in the three matrices showed that there was little difference in the degradation of the two enantiomers in wheat and cabbage, while R-(+)-penflufen was degraded preferentially in spinach. This study provides data supporting the scientific use and safety evaluation of penflufen.
研究领域:
植物学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）