Plants-Basel
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2023
·Vol. 12
·期 10
·1992
·
IF 4.5
Yongyao Fu,Liling Shu,Hanyi Li,Xingming Zhang,Xuan Liu,Zhengying Ou,Xiaomeng Liang,Xiangying Qi,Liping Yang
Lilium browniivar.viridulum, commonly called Longya lily, is a well-known flower and vegetable plant in China that has poor tolerance toBotrytisfungal disease. The molecularimprovement has mainly been restricted to an efficient regeneration and transformation system. In this study, the highly efficient regeneration of Longya lily was established through the optimization of embryogenic callus, adventitious shoot and rooting induction. The major factors influencing transformation (antibiotics,Agrobacteriumconcentration, infection time, suspension solution and coculture medium) were examined. The expression responses of PR promoters (ZmPR4andBjCHI1) toB. cinereawere assessed in transgenic calli. The results showed that Murashige and Skoog (MS) medium with 1.0 mg·L−1picloram (PIC) and 0.2 mg·L−11-naphthaleneacetic acid (NAA) under light conditions and MS with 0.5 mg·L−16-benzylaminopurine (6-BA) and 1.0 mg·L−1NAA under darkness were optimal for embryogenic callus induction (64.67% rate) and proliferation (3.96 coefficient). Callus inoculation into MS containing 2.0 mg·L−1thidiazuron (TDZ), 0.4 mg·L−1NAA, 1.0 mg·L−1TDZ and 0.5 mg·L−1NAA led to shooting induction (92.22 of rate) and proliferation (3.28 of coefficient) promotion, respectively. The rooting rate reached 99.00% on MS with 0.3 mg·L−1NAA. Moreover, a transformation rate of 65.56% was achieved by soaking the callus inAgrobacteriumat an OD600of 0.4 for 10 min in modified MS without NH4NO3as the suspension solution and coculture medium before selecting 75 mg·L−1hygromycin and 300 mg·L−1cefotaxime. Only theBjCHI1promoter was obviously expressed in transgenic calli. These results could facilitate the generation of Longya lily transgenic plants with improvedB. cinerearesistance.Keywords:Lilium browniivar.viridulum;plant regeneration;callus;genetic transformation;Botrytis cinerea
研究领域:
植物学
·
遗传学
·
植物病理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2018
·
IF 1.76
Fujuan Wei,Jin Wang,Shuai Huang,Bi Gong
Lilies ( Lilium spp.) are in demand worldwide because of their superior commercial and ornamental value. To develop new and practical preservation technologies, effects of pre-harvest treatment of Promalin (a.i. 1.8% each of 6-benzyladenine (6-BA) and gibberellic acid (GA 4 + 7 )) and 1-Methylcyclopropene (1-MCP) on cut lily under cold storage were investigated. Oriental lily ‘Sorbonne’ was selected for this study. The field plants at coloring stage were sprayed with 25 mg L −1 Promalin, 30 μL L −1 1-MCP additionally, their combination (1-MCP + Promalin) was also tested. Deionized water was used as control. The flower stems were harvested 24 h later and stored at 3 ± 1 °C. Preservation effect and physiological changes, e.g. inflorescence life, flower life, opening rate, dry weight loss rate, respiration rate, ethylene production rate, malondialdehyde (MDA) content, lipoxygenase (LOX) activity, energy metabolism related indexes including adenosine triphosphate (ATP) content, energy charge, activities of ATP synthase (ATPase), succinate dehydrogenase (SDH), cytochrome oxidase (CCO) and Ca 2+ -ATPase were determined. The results showed that 1-MCP or Promalin treatment extended the vase life of stored cut lilies and inhibited the decline of the dry weight of petals, reduced respiration rate, ethylene production rate, LOX activity and the accumulation of MDA. They also maintained the activity of ATPase, Ca 2+ -ATPase, SDH, CCO, ATP content and energy charge of cut lilies at a higher level than control. 1-MCP + Promalin exhibited an additive effect. Combination treatment maintained the flower opening ability as high as 89.3% after five-week storage, control merely maintained it till 3 weeks. These data indicate growth regulators (e.g. 1-MCP or Promalin) can extend the storage life of cut lily ‘Sorbonne’ through cutting down respiration consumption and manipulation of energy balance.
研究领域:
植物生理学
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园艺学
✓ 已验证
产品关联
DOI
高影响因子
Toxins
·
2023
·Vol. 15
·期 3
·209
·
IF 4.2
Yingyue Zhang,Fengyan Kuang,Chunyao Liu,Kai Ma,Tianyu Liu,Meijuan Zhao,Guangping Lv,He Huang
Edible and medicinal plants (EMPs) are widely used but are easily infected by harmful fungi which produce mycotoxins. Herein, 127 samples from 11 provinces were collected to investigate 15 mycotoxins based on geographic, demographic, processing, and risk characteristics. A total of 13 mycotoxins were detected, and aflatoxin B1(0.56~97.00 μg/kg), deoxynivalenol (9.41~1570.35 μg/kg), fumonisin B1(8.25~1875.77 μg/kg), fumonisin B2(2.74~543.01 μg/kg), ochratoxin A (0.62~19.30 μg/kg), and zearalenone (1.64~2376.58 μg/kg) occurred more frequently. Mycotoxin levels and species were significantly different by region, types of EMPs, and method of processing. The margin of exposure (MOE) values was well below the safe MOE (10,000). AFB1exposure from Coix seed and malt consumption in China was of high health concern. The hazard Index (HI) method showed the range of 113.15~130.73% for malt, indicating a public health concern. In conclusion, EMPs should be concerned because of the cumulative effects of co-occurred mycotoxins, and safety management strategies should be developed in follow-up studies.Keywords:mycotoxins;coix seed;lilii bulbus;malt;lotus seed;risk assessmentKey Contribution:This study investigated the co-occurrence of mycotoxins in EMPs from China, and indicated a high risk of mycotoxin exposure from Coix Seed and Malt. Mycotoxin contamination in EMPs varied significantly, and it may require more explicit limits for specific EMPs.
研究领域:
食品安全
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植物学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2017
·Vol. 28
·期 6
·3149-3158
·
IF 1.471
Du Juan,Zhou Zhie,Zhang Xueqing,Wu Shuang,Xiong Jianhua,Wang Wenjun,Luo Qiushui
Biosynthesis of gold nanoparticles (GNPs) by flavonoids from Lilium casa blanca has been developed. Several parameters such as pH, reaction temperature, reaction time and concentration of flavonoids were explored to control the formation of the GNPs. The synthesized GNPs were characterized by UV–Vis spectroscopy, transmission electron microscopy and X-ray diffraction. Stability and catalytic activity of the synthesized GNPs were also discussed. The results showed that the synthesized GNPs were in spherical, about 2.6 nm, with a face centered cubic structure. Synthesized GNPs showed good catalytic activity in the reduction of p -nitrophenol ( p -NP) to p -aminphenol ( p -AP). This method for synthesis of GNPs is simple, economic, nontoxic and efficient.
研究领域:
纳米生物学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Meiting Liu,Hongbin Pu,Da-Wen Sun
Microplastics that eventually convert into nanoplastics are emerging global pollutants and the development of efficient adsorbents for their removal is urgently needed. For sustainability and eco-friendliness, in the current study, a polysaccharide aerogel (LPA) was prepared with lily bulbs as the raw material by following water bath extraction, purification and freeze-drying processes. The prepared porous LPA was then applied as a packing material in a mini adsorption column for removing polystyrene (PS) microplastics and nanoplastics. Results revealed that LPA was over 90 kDa in size and consisted mainly of glucomannan and the removal efficiencies for microplastics and nanoplastics were 93.68 % and 96.98 %, respectively, mainly due to hydrogen bonding interactions and porous structure. The adsorption column was robust and maintained a remarkable removal efficiency (over 90 %) for 3 months. In addition, the effects of other extraction methods and pre-freezing conditions before the freeze-drying process were studied. Compared with water bath extraction, ultrasonic-assisted extraction and microwave-assisted extraction transformed the LPA structure, resulting in reduced adsorption ability, while the pre-freezing temperature could be used to adjust the specific surface area. Meanwhile, the effects of temperature and pH of adsorbates were also investigated. The LPA was heat sensitive and not stable under strongly acidic (pH 4) or strongly alkaline (pH 10) conditions, resulting in a sharp decline in removal efficiency. The adsorption behaviour of LPA was further described via adsorption kinetic models, showing that the microplastics and nanoplastics adsorptions could be fitted by pseudo-second-order and pseudo-first-order models, respectively. Moreover, the adsorption performance of LPA was compared with some other aerogels and had a better result. This research provides a promising, sustainable alternative for microplastic and nanoplastic removal that has potential for pollutant adsorption and sample purification as well as a low preparation cost.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Fenghua Jiang,Lei Zhou,Wei Zhou,Zhiwei Zhong,Kaibo Yu,Jing Xu,Liqiang Zou,Wei Liu
Fresh-cut lily bulbs have broad consumption prospects in the vegetable market due to their high nutritional value and convenience in processing; however, potential discoloration and microbial infection during storage adversely affect their quality. This study investigated the effect of modified atmosphere packaging (MAP) and plant essential oils (EOs) on the preservation of lily bulbs. The results showed that MAP significantly preserved the color and weight of lily bulbs, and different MAP conditions caused different effects on bacterial inhibition. Low oxygen-MAP (L-MAP) showed a stronger antibacterial effect than air-MAP (A-MAP), whereas appropriate antibacterial agents were still needed to improve the antibacterial effect. Therefore, plant EOs were employed to further control the growth of microorganisms by preparing plant EO solid particles (PESP). The combined use of L-MAP and PESP significantly prolonged the shelf life of lily bulbs to 12 d with higher contents of nutrients and better appearance quality compared to the control. Furthermore, the physiological metabolism of lily bulbs was regulated by combined treatment with L-MAP and PESP, which inhibited starch degradation, MDA accumulation and respiratory oxidase activities.
研究领域:
食品科学
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植物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Li Zhou,Yuting Guan,Jiaxu Yao,Minjie Zhao,Haiyan Fu,Jikai Liu,Eric Marchioni
Lilium brownii var. viridulum Baker is an economically important plant, and its bulbs are popular among consumers for their high nutritional value. Due to the influence of environment, the chemical composition of lily from different origin is different, which leads to the different medicinal and nutritional values. Thus, it is very significant and necessary to discriminate the origins of lily. In this study, lipid classes and molecular species of lily samples from eight origins were identified and quantified by UHPLC-QE-Orbitrap/MS/MS and combined with chemometrics for modeling and analysis. The results showed that a total of 16 lipid classes and 88 lipid molecules were identified and quantified. The partial least squares discriminant analysis (PLS-DA) showed that there were significant differences in lipid metabolites between LH (Daodi) and other origins. And 39 characteristic compounds such as TAG 16:0/18:0/18:2, TAG 18:0/18:1/18:2, and LDGCC 15:2 could be used as the markers to identify the geographical origin of lily. This study established a new method to identify the origin traceability of lily in different production areas, and provided a theoretical basis to evaluate the nutritional value of lilies from different origins.
研究领域:
植物化学
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代谢组学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 14
·2206
·
IF 4.7
Lixia Xie,Jiajia Liu,Haoyu Wu,Yueyan Zhong,Xueying Liu,Guangli Li,Zhi Liu
The present study aimed to investigate the variations in the nutritional composition, antioxidant capacity, and metabolite profile of lilies subjected to different drying treatments, including vacuum freeze drying (VFD), hot air drying (HAD), vacuum drying (VD), and infrared drying (ID). The results show that VFD provided better preservation of the original coloration and displayed the highest levels of total amino acid content, total phenolic content, total flavonoid content, and polysaccharide and alkaloid content. Our results reveal that VFD treatment can be employed to obtain high-quality lilies with desirable appearance characteristics and nutrient compositions. Metabolomics analysis identified a total of 464 metabolites from various dried lilies. Differential metabolite screening found 150 differential metabolites across all pairwise comparisons. Hierarchical clustering analysis (HCA) indicated that lilies subjected to VFD treatment exhibited a higher abundance of steroids, saponin, flavonoids, and phenolic glycoside, whereas those subjected to HAD, VD, or ID treatments showed relatively elevated levels of specific amino acids or derivatives. This study elucidates the significant impact of various drying treatments on the quality and metabolic profile of lilies, thereby providing valuable insights for enhancing the nutritional quality of processed lilies.
研究领域:
植物学
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食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Micro & Nano Letters
·
2019
·Vol. 14
·期 10
·1069-1074
·
IF 0.975
Haimin Li,Wenjun Wang,Beijun Ang,Jianhua Xiong,Fengying Zhang,Weiping Chen,Juan Du
A simple, fast, and eco-friendly method for the biosynthesis of gold nanoparticles (AuNPs) using Lilium casa blanca petals as a reducing and protecting agent was reported. The heating time had been shortened to 30 min for biosynthesis of AuNPs, which only took 15 mg Lilium casa blanca petals powder. The biosynthetic AuNPs, which had a uniform particle size distribution, were in spherical with the average size of 5.933 ± 1.158 nm. In addition, the conversion rate of Au 3+ to Au 0 was quite high. Through the discussion on the nutrients' concentrations before and after the synthesis, it was found that sugars, alkaloids, flavonoids, and proteins were the reducing and stabilising agents. The biosynthetic AuNPs had good catalytic activity, taking the hydroboration of nitrophenol and methylene blue as examples. Catalytic reduction followed pseudo-first-order kinetics. Moreover, the apparent rate constant was a linear correlation with the concentrations of AuNPs. The normalised rate constant of the proposed AuNPs was higher than other reported AuNPs, which indicated the excellent catalytic activity. All the results foreshowed the wide range of applications of the biosynthetic AuNPs by Lilium casa blanca petals.
研究领域:
纳米生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 18
·期 10
·e2100403
·
IF 2.408
Jie Yan,Wenxiu Guo,Lanyu Zhou,Dale Guo,Jin Pei,Yun Deng,Haoyuan Zheng,Ding Liu,Xiaofang Xie,Cheng Peng
Three previously undescribed chlorophenyl glycosides, (2,4,6-trichloro-3-hydroxy-5-methoxyphenyl)methyl β-D-glucopyranoside ( 1 ), (2,4-dichloro-3,5-dimethoxyphenyl)methyl 6- O -β-D-glucopyranosyl-β-D-glucopyranoside ( 2 ) and 4-chloro-3-methoxy-5-methylphenyl 6- O -(6-deoxy-β-L-mannopyranosyl)-β-D-glucopyranoside ( 3 ) were obtained from Lilium regale . The absolute configurations of these new finds were elucidated by comprehensive analyses of spectroscopic data combined with acid hydrolysis derivatization. (2,4-dichloro-3,5-dimethoxyphenyl)methyl 6- O -β-D-glucopyranosyl-β-D-glucopyranoside ( 2 ) can inhibit the proliferation of lung carcinoma A549 cells with an IC 50 value of 29 μΜ. Graphical
研究领域:
植物化学
·
药理学
·
天然产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·
IF 1.303
Yun Wu,Yi-ping Xia,Jia-ping Zhang,Fang Du,Lin Zhang,Yi-di Ma,Hong Zhou
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Lilium Oriental Hybrids ‘Sorbonne’ under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (≥2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5'-diphosphate glucose pyrophosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm, respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.
研究领域:
植物学
·
园艺学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Haiwei Ren,Zhihang Xu,Ming Gao,Xueye Xing,Zhe Ling,Lichao Pan,Yaqin Tian,Yi Zheng,Wenguang Fan,Weixia Yang
Microcrystalline celluloses were isolated from four agricultural residues, including sweet sorghum stalk, Jerusalem artichoke stalk, grains stillage, and Chinese herb residue, and characterized in terms of physicochemical and structural properties. The obtained microcrystalline celluloses were composited with polylactic acid as a packing film for the preservation of Lanzhou lily. All the agricultural residues-derived microcrystalline celluloses were in cellulose I β structure with high purity and good thermal stability . Microcrystalline celluloses from sweet sorghum stalk had a higher degree of polymerization (327) and crystallinity (70.52 %) than others. The preservation effect of lily bulbs packaged by films were significantly improved indicated by the lessened weight loss rate and the meliorative hardness and whiteness, which ascribe to the repressed oxidation reactions . Polylactic acid/microcrystalline cellulose composite films prepared from sweet sorghum straw have been proved the most effective. This work could offer a value-added outlet for agricultural residues to produce microcrystalline celluloses-based biocompatible films for preservation of Lanzhou lily.
研究领域:
材料科学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）