BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2015
·Vol. 38
·期 7
·1049-1053
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IF 1.828
Zhiyi Wang,Jianshe Ma,Meiling Zhang,Congcong Wen,Xueli Huang,Fa Sun,Shuanghu Wang,Lufeng Hu,Guanyang Lin,Xianqin Wang
Paraquat is one of the most widely used herbicides in the world and is highly toxic to humans and animals. In this study, we developed a serum metabolomic method based on GC/MS to evaluate the effects of acute paraquat poisoning on rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate analysis revealed that acute paraquat poisoning induced metabolic perturbations. Compared with the control group, the level of octadecanoic acid, L-serine, L-threonine, L-valine, and glycerol in the acute paraquat poisoning group (36 mg/kg) increased, while the levels of hexadecanoic acid, D-galactose, and decanoic acid decreased. These findings provide an overview of systematic responses to paraquat exposure and metabolomic insight into the toxicological mechanism of paraquat. Our results indicate that metabolomic methods based on GC/MS may be useful to elucidate the mechanism of acute paraquat poisoning through the exploration of biomarkers.
研究领域:
毒理学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
·
IF 6.1
Siqi Yu,Fudong Wang,Yuan Zhang,Xiaofeng Wang,Hongyu Zhao,Qiang Zheng
Autophagy inhibition induced by paraquat (PQ) poisoning is an important factor causing multi-organ damage, including renal tissue. Dihydrocapsaicin (DHC), the main active ingredient in chili peppers, was reported to induce autophagy, but its effect on PQ-induced AKI remains unclear. Rats were intraperitoneally injected with 25 mg/kg PQ to establish AKI rat model followed immediately by DHC administration (2.5, 5, or 10 mg/kg, i.p.). Rat renal tubular epithelial cells were exposed to PQ (300 μM) in combination with DHC (25, 50, or 100 μM) for 24 h. The results showed that DHC treatment attenuated PQ-induced renal injury and dysfunction while suppressing apoptosis and oxidative stress in vivo and in vitro . Furthermore, DHC administration induced autophagy—a protective mechanism against oxidative stress and apoptosis—as demonstrated by: 1) increased autophagosome/autophagolysosome formation, 2) upregulated LC3-II and autophagy-related gene 7 expressions and reduced p62 protein level. Blocking of autophagy by 3-methyladenine (3-MA) reversed the effects of DHC. Interestingly, transcription factor sex determining region Y-box 9 (Sox9) and autophagy inducer Sestrin2 RNA levels were increased by DHC treatment. Sox9 activated Sestrin2 transcription through direct binding to its promoter region. Sox9 or Sestrin2 silencing reversed the effects of DHC. Collectively, our findings demonstrate that DHC attenuates PQ-induced AKI via Sox9/Sestrin2 axis-mediated autophagy, indicating therapeutic utility of DHC against AKI.
研究领域:
药理学
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毒理学
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分子生物学
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肾脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 37
·4562-4571
·
IF 2.596
Wen Liu,Sha Li,Yang Ke Wu,Xiao Yan,Yi Ming Zhu,Fei Ya Jiang,Yu Jiang,Liang Hong Zou,Tong Tong Wang
Xuebijing (XBJ) is a compound Chinese medicine that contains Paeoniae Radix Rubra, ChuanXiong Rhizoma, Salvia Miltiorrhiza Radix et Rhizoma, Carthami Flos, and Angelicae Sinensis Radix. It is widely used in China to treat sepsis. Previous studies have demonstrated that XBJ can decrease mortality in patients with moderate paraquat poisoning. However, the mechanism by which it exerts this effect is not completely clear. In this study, an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)-based metabolomics approach was used to perform a metabolic profiling analysis. Principal component analysis (PCA), random forest (RF), and partial least squares discriminant analysis (PLS-DA) were used to identify metabolites to clarify the mechanism of XBJ's activity. XBJ clearly alleviated lung injury in a Sprague Dawley (SD) rat model of paraquat (PQ) poisoning. Seven metabolites related to four pathways, including those involved in sphingolipid and phospholipid metabolism, amino acid metabolism, unsaturated fatty acid metabolism, and pantothenic acid and CoA biosynthesis, were present at different levels in PQ-poisoned rats treated with XBJ compared with untreated rats. XBJ can ameliorate the effects of PQ poisoning in SD rats. Using a metabolomics approach enabled us to gain new insight into the mechanism underlying this effect.
研究领域:
药理学
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毒理学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Hepatobiliary & Pancreatic Diseases International
·
2025
·
IF 4.4
Han-Ying Zhou,Ting Li,Yuan-Qiang Lu
Background Diquat, a commonly employed bipyridyl herbicide, is recognized for its hepatotoxic effects attributed to the generation of reactive oxygen species. Baicalin (BAI), a flavonoid derivative, has garnered significant research interest for its hepatoprotective properties. Nevertheless, the clinical application of BAI is constrained by its limited water solubility and poor bioavailability. To address these challenges, BAI-nanoliposome (BAI-NL) has emerged as a novel drug delivery platform aimed at enhancing therapeutic outcomes. Methods We used diquat-induced liver injury mouse model and AML12 hepatocytes to test the protective effect of BAI and BAI-NL on liver inflammation, oxidative stress, and mitochondrial function. The parameters included histological, biochemical, and molecular biological analyses. Results In the diquat-induced model, both BAI and BAI-NL exhibited effectiveness on attenuating liver inflammation. Ex vivo analyses further indicated that BAI-NL was superior to BAI in preserving mitochondrial membrane potential, reducing oxidative stress, and modulating the phosphatase and tensin homolog-induced putative kinase 1 (PINK1)/Parkin RBR E3 ubiquitin-protein ligase (Parkin) signaling pathway. These findings enhanced mitophagy and facilitated the removal of damaged mitochondria. Conclusions BAI-NL exhibited superior hepatoprotective effects compared to free BAI, possibly by reducing inflammation, preserving mitochondrial homeostasis, and reinstating autophagic balance through modulation of the PINK1/Parkin signaling pathway. These outcomes indicate a groundbreaking method for addressing liver diseases and underscore the potential of nanoliposome technology in augmenting the efficacy of natural compounds.
研究领域:
药理学
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毒理学
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CLAY MINERALS
·
2024
·
IF 1.1
Rui Liu,Chao Ma,Huanliang Chen,Changsuo Li,Lei Zuo,Peng Zhang,Yaqing Wang,Ru Wang
Clay minerals are effective adsorbents for the remediation of pesticides in wastewater due to their large superficial areas and excellent cation-exchange capabilities. However, this adsorption effect can be reduced by the accumulation of adsorbents on clay minerals, amongst other problems. Therefore, in this study, montmorillonite (Mnt) modified by layered double hydroxide (LDH) with different loading amounts was successfully prepared using an in situ method. The results from X-ray diffraction, Fourier-transform infrared spectrometry, Brunauer–Emmet–Teller (BET) and scanning electron microscopy analyses revealed that LDH structures were successfully combined with the Mnt layer and formed a porous structure. However, excess LDH still caused the aggregation and accumulation of layers. The adsorption performance of LDH@Mnt for atrazine (ATZ) and paraquat (PQ) was investigated, and the removal efficiency of the LDH@Mnt composite was higher than those of Mnt and LDH alone. The kinetic study revealed that the adsorption process fitted the pseudo-second-order model and internal diffusion model, and 3-LDH@Mnt had the greatest absorbability efficiency for both ATZ and PQ, indicating the adsorption process was controlled by the number of active sites of the adsorbent. The generalized Langmuir model accurately characterized the adsorption process of ATZ and PQ elimination in the adsorption isotherm investigation, indicating that the adsorption energies of the active sites on the adsorbents were different. 3-LDH@Mnt had better absorbability performance for ATZ/PQ, and the sorption capacities were 7.03 and 91.9 mg g–1, respectively. According to site energy distribution theory, the amount of sorption sites of the composite adsorbent was large and the average adsorption energy was high, both of which being beneficial for the adsorption of ATZ and PQ. The effects of pH, coexisting anions and reuse experiments were also tested, indicating that the LDH@Mnt composite possessed high adsorption stability. This excellent removal performance represents a promising strategy for the remediation and elimination of pesticide contaminations from the environment.
研究领域:
环境科学
·
土壤学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Liling Zeng,Xianyan Ao,Manli Xu,Yunqian Zhang,Zhu Tao
The synergistic removal of organic pollutants by adsorption and photocatalysis can be achieved by adsorption of enriched pollutants, followed by photocatalytic degradation to decompose the pollutants into non-toxic substances. In this work, a category of adsorption-photocatalytic bifunctional composite materials Q[6]-HPAs have been developed through the self-assembly of cucurbit[6]uril (Q[6]) and heteropoly acids (HPAs) like phosphomolybdic acid (PMA), phosphotungstic acid (PTA) and silicotungstic acid (STA). These composites exhibit enhanced surface area, structural stability, and extended light absorption into the visible range. Notably, Q[6]-HPAs have surface charge properties that attract cations and an energy band structure suitable for the generation of reactive oxygen species, thus possessing the potential for efficient adsorption and photodegradation of cationic pesticides. Among which, Q[6]-PMA and Q[6]-PTA removed over 95 % of diquat and paraquat through sequential adsorption and photodegradation. Electrostatic attraction drives the adsorption process, and ·O 2 − and ·OH radicals detected via Electron Spin Resonance (ESR) are the key to photodegradation. Ultimately, Liquid Chromatography-Mass Spectrometer (LC-MS) analysis revealed potential degradation intermediates and products, and toxicity analysis was performed.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Siying Che,Xiutan Peng,Yiwan Zhuge,Xinlan Chen,ChunSong Zhou,Haiyan Fu,Yuanbin She
Detection of pesticide paraquat (PQ) is of considerable significance to ensure food safety, and its rapid and on-site detection is still a challenge. Aimed at the ion characteristics of PQ, an “enrichment and detection” strategy was proposed to improve the sensitivity through electrostatic attractions, and the ion characteristic of probes was adopted to increase the portability through avoiding aggregation-caused quenching effects in the paper strips. Herein, a novel anion-functionalized ionic liquid (IL) probe with a large conjugated plane and rich π-electrons ([Fluo][P66614]2) was designed as a fluorescent and colorimetric dual-channel probe to sensitively and rapidly detect trace amounts of PQ in vegetables and the environment. The proposed probe exhibited good linearity with a detection limit of 64.0 nM in the PQ concentration range of 0.3–7.0 μM (fluorometry) and 0.1 μM in that of 0.1–8.0 μM (colorimetry), respectively. In addition, it displayed a rapid fluorescence quenching response from green to dark (<5 s) and excellent anti-interference (among 23 other pesticides) due to dual effects of electrostatic attraction and π–π stacking. Most importantly, the lipophilic IL probe could be applied in real vegetables and environmental samples with a satisfying recovery rate of 98–103% and assembled into a handy paper strip that achieved the visual semiquantitative detection of PQ. This ionic probe provides a feasible approach for rapidly and conveniently detecting PQ for ensuring agricultural and food safety and opens a new avenue to detect ion-responsive analytes in real complex samples by an “enrichment and detection” strategy.
研究领域:
分析化学
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食品安全
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环境科学
✓ 已验证
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Zheng Zhao,Xue Yang
Paraquat (PQ) poisoning leads to irreversible fibrosis in the lungs with high mortality and no known antidote. In this study, we investigated the effect of the SET and MYND domain containing 2 (SMYD2) on PQ-induced pulmonary fibrosis (PF) and its potential mechanisms. We established an in vivo PQ-induced PF mouse model by intraperitoneal injection of PQ (20 mg/kg) and in vitro PQ (25 μM)-injured MLE-12 cell model. On the 15th day of administration, tissue injury, inflammation, and fibrosis in mice were evaluated using various methods including routine blood counts, blood biochemistry, blood gas analysis, western blotting, H&E staining, ELISA, Masson staining, and immunofluorescence. The findings indicated that AZ505 administration mitigated tissue damage, inflammation, and collagen deposition in PQ-poisoned mice. Mechanistically, both in vivo and in vitro experiments revealed that AZ505 treatment suppressed the PQ-induced epithelial-mesenchymal transition (EMT) process by downregulating GLI pathogenesis related 2 (GLIPR2) and ERK/p38 pathway. Further investigations demonstrated that SMYD2 inhibition decreased GLIPR2 methylation and facilitated GLIPR2 ubiquitination, leading to GLIPR2 destabilization in PQ-exposed MLE-12 cells. Moreover, rescue experiments conducted in vitro demonstrated that GLIPR2 overexpression eliminated the inhibitory effect of AZ505 on the ERK/p38 pathway and EMT. Our results reveal that the SMYD2 inhibitor AZ505 may act as a novel therapeutic candidate to suppress the EMT process by modulating the GLIPR2/ERK/p38 axis in PQ-induced PF.
研究领域:
药理学
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毒理学
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细胞生物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2017
·
IF 5.099
Xiaoji Fan,Jiahui Xu,Michel Lavoie,W.J.G.M. Peijnenburg,Youchao Zhu,Tao Lu,Zhengwei Fu,Tingheng Zhu,Haifeng Qian
Carbon nanotubes can be either toxic or beneficial to plant growth and can also modulate toxicity of organic contaminants through surface sorption . The complex interacting toxic effects of carbon nanotubes and organic contaminants in plants have received little attention in the literature to date. In this study, the toxicity of multiwall carbon nanotubes (MWCNT, 50 mg/L) and paraquat (MV, 0.82 mg/L), separately or in combination, were evaluated at the physiological and the proteomic level in Arabidopsis thaliana for 7–14 days. The results revealed that the exposure to MWCNT had no inhibitory effect on the growth of shoots and leaves. Rather, MWCNT stimulated the relative electron transport rate and the effective photochemical quantum yield of PSII value as compared to the control by around 12% and lateral root production up to nearly 4-fold as compared to the control. The protective effect of MWCNT on MV toxicity on the root surface area could be quantitatively explained by the extent of MV adsorption on MWCNT and was related to stimulation of photosynthesis, antioxidant protection and number and area of lateral roots which in turn helped nutrient assimilation. The influence of MWCNT and MV on photosynthesis and oxidative stress at the physiological level was consistent with the proteomics analysis, with various over-expressed photosynthesis-related proteins (by more than 2 folds) and various under-expressed oxidative stress related proteins (by about 2–3 folds). This study brings new insights into the interactive effects of two xenobiotics (MWCNT and MV) on the physiology of a model plant.
研究领域:
植物学
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环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Hong Zeng,Hongli Yang,Yue Tang,Xiaojuan Niu,Yuangen Wu
A facile and simple colorimetric biosensor was first established for paraquat (PQ) detection based on the aptamer-enhanced oxidation process of 3,3′,5,5′-tetramethylbenzidine (TMB) by Ag + . The study confirmed that the interaction of PQ-15 aptamer with Ag + accelerates the electron transfer from the aptamer-Ag + complex to dissolved oxygen, which enhances the release of superoxide anion radicals (O 2 ̇ − ) and facilitates the catalytic oxidation of the chromogenic substrate. PQ-15 aptamer will preferentially bind to PQ molecules, resulting in no further enhancement of the catalytic activity of Ag + . Molecular docking results revealed that the PQ molecules are attached to the stem-loop region of the PQ-15 aptamer through σ-π conjugation interactions. The proposed method is simple that only contains Ag + and corresponding aptamer. The limit of detection (LOD) of the constructed colorimetric biosensor for PQ detection was determined to be 16.5 μg·L -1 , belowing the maximum residue limit in fruits and vegetables set by the EU. Moreover, the colorimetric biosensor showed excellent selectivity and anti-interference properties, which was validated for detecting PQ residues in several typical agricultural and water samples.
研究领域:
生物化学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2022
·Vol. 2022
·期 1
·1254824
·
IF 3.246
Jielun Yu,Lichun Zhang,Xiaoshuang Li,Kaixuan Lv,Shiyu Sun,Weihua Wu,Lifeng Ping,Guifang Guo,Wei Tan,Shoudong Guo,Kezhou Wang,Aihua Zhao,Nana Yang
Background . This study was designed to investigate differences in biochemical parameters between mouse and humans after paraquat (PQ) poisoning and develop a suitable animal model for studying organ damage after PQ poisoning. The prognostic factors of PQ-poisoned patients were further analyzed. Methods . Thirty C57BL/6J mice were randomly divided into five groups (control, sham, and 3 PQ doses), and the mouse model was established by intragastric administration of PQ. Physiological indexes such as the body weight, mental state, and mortality rate were observed. Biochemical parameters were analyzed 24 h after PQ poisoning. We also performed a retrospective analysis of clinical data from 29 patients with PQ poisoning admitted to the Emergency Department of the Affiliated Hospital of Taishan Medical College between April 2016 and February 2018. Biochemical parameters were compared between the mouse model and patients with PQ poisoning. Results . In the PQ poisoning mouse model, the lethal dose group PQ360 showed remarkable increases in serum levels of potassium (K + ), carbon dioxide (CO 2 ), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) compared with the nonlethal dose PQ100 and PQ200 groups. The biochemical results of the patients showed that K + and Cl - levels were significantly reduced in the death group compared to the survival group. Levels of ALT, AST, blood urea nitrogen (BUN), and amylase were higher, and the neutrophil-to-lymphocyte ratio (NLR) was increased in the death group compared with the survival group. Conclusions . The combination of age, PQ dosage, K + , Cl - , BUN, ALT, AST, amylase, and NLR can be used to more accurately predict the outcome of patients with PQ poisoning. C57 mice are an appropriate animal model to study liver and kidney functions following PQ exposure.
研究领域:
毒理学
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医学
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实验动物学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Haijie Li,Hao Qi,Mingyuan Yin,Yujie Chen,Qiliang Deng,Shuo Wang
Paraquat (PQ) pollution has become an increasing seriously threat to the ecological environment. Thus, it is vital to study novel adsorption materials capable of removing PQ. Modified carbon tubes (MCT-600) were prepared by carbonizing the seed hair fibers of Metaplexis japonica at 600 °C and further modifying with nitric acid . The MCT-600 was found to possess a regular morphology, a good graphitization degree and an abundance of carboxyl functional groups. Moreover, this materil exhibits excellent adsorption performance for paraquat (PQ), such as a fast adsorption rate (adsorption equilibrium within 20 min), large adsorption capacity (218.61 mg g −1 ) and good regeneration ability (reused more than 5 times). The adsorption process for PQ onto MCT-600 was found to be well matched to a Langmuir isotherm and pseudo-second-order kinetics model. Owing to its unique surface properties, green raw material source and simple preparation process, MCT-600 shows potential as an outstanding candidate for PQ removal from water.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）