Frontiers in Microbiology
·
2019
·
IF 4.259
Chen Xiao,Zhang Zheng,Liu Xiaoxia,Cui Bo,Miao Wentao,Cheng Weiwei,Zhao Fengyun
Volvariella volvacea is a typical edible Basidiomycete with a high-temperature tolerance. It has a strong fibrinolysis capability and consumes abundant agricultural wastes. In agricultural cultivation, mycelial subculturing has been adopted, leading to serious strain degeneration. In this study, continuous mycelial subculturing of the common V. volvacea strain V971 (original strain recorded as M0) was performed in potato dextrose agar (PDA) medium. One generation of the strain was preserved every 3 months (90 days); thus, six generations of degenerated strains (M1–M6) were obtained after 18 months of mycelial subculturing. The original and degenerated strains were preserved in sterile paraffin liquid at room temperature (18–25°C). The biological traits and nutrients of M0 and M1–M6 were studied. The mycelial growth rate and biomass initially increased and then decreased as the degeneration progressed, reaching minimum levels of 0.041 ± 0.001 cm/h and 1.82 ± 0.25 g, respectively, at M6. Additionally, the polysaccharide, protein, polyphenol, flavone, total amino acid, and total mineral element contents of the strains decreased continuously, reaching minimum levels of 30.12 ± 3.12 g/100 g, 26.42 ± 2.1 g/100 g, 1.08 ± 0.05 g/100 g, 4.23 ± 0.21 g/100 g, 12.51 mg/g, and 398.05 mg/kg, respectively, at M6. The decolorization capability of V. volvacea in liquid medium supplemented with bromothymol blue and lactose reflected the degree of strain degeneration, with the capability weakening as the degeneration intensified. These results are highly significant for V. volvacea production. The mycelial characteristics during subculture-associated degeneration were described and provide an early identification method for V. volvacea’s degeneration.
研究领域:
真菌学
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食用菌研究
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农业微生物学
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2021
·Vol. 16
·期 10
·500-505
·
IF 1.102
Dejuan Fa,Yuqing Miao
By hydrothermal reaction, the hydrangea-like ZnS/ZnIn 2 S 4 microspheres were prepared in the presence of thioacetamide. X-ray photoelectron spectroscopy and high resolution transmission electron microscopy confirmed that the ultra-long nanowires are composed of ZnS and ZnIn 2 S 4 . Scanning electron microscopy showed the morphology of ultra-thin nanosheets with an average thickness of 8.5 nm cross from inside to outside, forming ZnS/ZnIn 2 S 4 microspheres with a porous diameter of about 2.8 μm. Nitrogen adsorption desorption isotherm determined that the BET specific surface area of the microspheres was 109.19 m 2 /g which showed that they have a large specific surface area. The obtained ZnS/ZnIn 2 S 4 microspheres showed broad absorbance ranging from UV (200 nm) to visible (520 nm) with low indirect band gaps of 2.08 eV. It leads to outstanding visible-light-driven photocatalysis for the degradation of xylenol orange (XO) and thymol blue (TB) in water, and the two dyes were almost completely degraded after 60 and 135 min, respectively. This provides new potential to explore nanomaterials for efficient photo-driven degradation of organic dyes under visible light irradiation.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Danmin Yang,Qun Liu,Xu Zeng,Xiaoting Chen,Meng Li,Xialing Wu,Yue Liu,Yanzhen Zheng,Jionghua Xiang,Chunchun Wang,Wuyin Weng,Yucang Zhang
The cellulose nanofibers (CNFs) based pH-sensitive indicator films were developed by mixing guar gum (GG) with bromothymol blue-anchored chitin (BTB-Chitin) as an indicator to monitor shrimp freshness. The BTB-Chitin was prepared by grafting hydroxypropyltriethylamine groups (HPTA) to chitin first, then anchoring bromothymol blue (BTB) to prepare intelligent pH response BTB-Chitin. The 0.08 BTB-Chitin films had a good tensile strength of 11.76 MPa and the water contact angle values were 125°, which displayed obvious color response to pH buffers and acid base volatile gas. Besides, the homogeneous and flexible composite films showed good color stability and reversibility. The released amount of BTB was very low from the BTB-Chitin films in heptane and corn oil. The composite films had been degraded completely in 15 days in soil. The pH and volatile base nitrogen were measured to determine the degree decay of shrimp ( Litopenaeus vannamei ), and the prepared pH-sensitive films changed from yellow (fresh) to cyan (spoiled) with the freshness of shrimp decreased, indicating the BTB-Chitin films could detect the shrimp freshness in real-time and high visibility.
研究领域:
食品科学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2015
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IF 2.729
Xiaoqian Ouyang,Liqiang Luo,Yaping Ding,Bingdi Liu,Duo Xu,Anquan Huang
A glassy carbon electrode modified with poly(bromocresol green) was prepared by electropolymerization process for simultaneous determination of uric acid, dopamine and ascorbic acid. The interface morphology and structure of poly(bromocresol green) film were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The simultaneous determination of uric acid (UA), dopamine (DA) and ascorbic acid (AA) in 0.1 M phosphate buffer solution (pH 6.0) was carried out by differential pulse voltammetric technique. Under optimum conditions, the results show that the peaks of three species were well separated. The proposed sensor exhibited linear responses to UA, DA and AA in the ranges of 0.5–200, 0.05–10 and 0.5–1000 μM, respectively. Moreover, the poly(bromocresol green) modified electrode has been successfully applied to determine UA, DA and AA in human serum samples and vitamin C tablets.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
CHEMICAL PHYSICS LETTERS
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2019
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IF 1.901
Zhihao Wang,Yihe Zhang,Wenjiang Wang,Qi An,Wangshu Tong
In this study, three minerals were chosen as the host of colorimetric humidity sensor, and Bromothymol blue (BTB) was used as the indicator. The BTB based on titanium dioxide (BTB-TiO 2 ), BTB based on palygorskite (BTB-palygorskite) and BTB based on mullite (BTB-mullite) exhibited the readable response to the relative humidity (RH) of 11, 43 and 75% at 1 min at 25 °C. Moreover, the BTB-palygorskite exhibited the rapid and stable color response, especially under the low humidity (11% RH). The surface area played an important role on the cover density of BTB and water adsorption which made the humidity sensor with excellent performances.
研究领域:
材料科学
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化学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Chen Hou,Shengyang Zou,Daozhen Cheng,Junyi Gao,Tao Fu,Jianzhi Wang,Yang Wang
Developing high-performance multifunctional catalyst that adept at both sensitive detecting and efficient degrading of volatile organic compounds (VOCs) is of significant importance for human and eco safety. This work presented a bifunctional nonmetal composite membrane by functionalizing transparent cellulose membrane with double responsive bromocresol green and catalytic g-C 3 N 4 /COF TAPB-PDA (CNC) heterojunctions for simultaneous detection and degradation of amine VOCs. Bromocresol green possesses both intrinsic visualized acid-base responsiveness and wide light adsorption range. Hierarchical CNC with high specific surface area (up to 1256.60 m 2 g −1 ) can effectively promote the absorption and diffusion of substrates and the separation of photo-generated carriers. A flexible macroporous transparent cellulose membrane was applied as carrier to alleviate the “self-masking effect” of the catalyst inside to improve light utilization. As a result, the composite membrane showing a visually reversible color change from yellow to blue for amine VOCs and achieving rapid quantitative detection of representative triethylamine (detection time of 5 s with a LOD of 0.32 ppm) on the aid of a smart phone. The degradation and mineralization efficiency of triethylamine reached 97.3 % and 73.5 % within 180 min, respectively. The ingenious design of integrating bifunctional cellulose composite material provides feasibility for the rapid identification and efficient removal of VOCs.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 4
·663
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IF 3.06
Kai Song,Yejing Wang,Yu Li,Chaoxiang Ding,Rui Cai,Gang Tao,Ping Zhao,Qingyou Xia,Huawei He
Enzymatic activity assays are essential and critical for the study of enzyme kinetics. Adenylate kinase (Adk) plays a fundamental role in cellular energy and nucleotide homeostasis. To date, assays based on different principles have been used for the determination of Adk activity. Here, we show a spectrophotometric analysis technique to determine Adk activity with bromothymol blue as a pH indicator. We analyzed the effects of substrates and the pH indicator on the assay using orthogonal design and then established the most optimal assay for Adk activity. Subsequently, we evaluated the thermostability of Adk and the inhibitory effect of KCl on Adk activity with this assay. Our results show that this assay is simple, rapid, and precise. It shows great potential as an alternative to the conventional Adk activity assay. Our results also suggest that orthogonal design is an effective approach, which is very suitable for the optimization of complex enzyme reaction conditions.Keywords:enzymatic activity assay;adenylate kinase;spectrophotometry;orthogonal experiment;bromothymol blue
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2018
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IF 2.559
Yu Li,Yejing Wang,Kai Song,Lina Liu,Rui Cai,Gang Tao,Ping Zhao,Haimeng Zhou,Huawei He
Adenosine kinase (ADK) plays an important role in the growth and development of organisms. A convenient, quick, reliable, sensitive and low-cost assay for ADK activity is of great significance. Here, we found the reaction system with bromothymol blue as the pH indicator had a maximum absorption peak at 614 nm. The absorbance change in 614 nm was positively correlated with the generated hydrogen ions in the reaction catalyzed by ADK. Then, we demonstrated this assay was feasible for ADK activity. Further, we analyzed the effects of buffer, bromothymol blue concentrations on the sensitivity of the assay, and investigated the sensitivity of ADK contents and adenosine concentration on the assay. Finally, we calculated the K m and Vmax of ADK from Bombyx mori with this assay. Our results suggested this assay was quick, convenient, reliable, sensitive and economic for the activity of ADK. It is an excellent alternative for the conventional ADK assays.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Muthuchamy Maruthupandy,Govindan Rajivgandhi,Thillaichidambaram Muneeswaran,Seerangaraj Vasantharaj,Gabriela Sandoval-Hevia,Muniyandi Muneeswaran,Nurul Asyikin Binti Mohamed Radzi
The pervasive occurrence of various organic contaminants, primarily dye molecules, in water bodies was caused by multiple dye industrial wastes, which recently sparked great scientific concern and public awareness due to their potential to spread these contaminants' resistant genes and pose a risk to humans. Cross-linking graphene in chitosan resulted in nanocomposites with varied morphology, increased surface area, and improved photocatalytic removal of bromothymol blue and bromophenol blue. The degradation capacity of photocatalysts at various concentrations (25 and 50 mg) was 96.2 % (170 min) and 99.2 % (80 min) with bromothymol blue, revealing a two-fold variation based on the Gr/Cts NCs concentrations. Similar to bromophenol blue dye molecules, which showed a two-fold enhancement of degradation based on graphene/chitosan nanocomposites concentration, bromophenol blue dye molecules were 95.3 % and 98.6 % at 240 and 120 min time intervals. The bromophenol blue and bromothymol blue dyes degraded according to the Langmuir adsorption model. Furthermore, the minimal cytotoxicity of graphene/chitosan nanocomposites to the human lung epithelial cell line and the human macrophage-like cell line indicates that the material is safe. This study reveals that the degrading properties of graphene/chitosan nanocomposites significantly improve as the concentration increases.
研究领域:
环境科学
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材料科学
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生物学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
·
IF 6.1
Min Wang,Yuanzhang Jiang,Kaiwen Zhuang,Lin Tan
Skin infections are a major threat to human health. Early diagnosis of bacterial infections is of great significance for implementing protective measures on the skin. Therefore, in this study, we designed an electrospun membrane (PPBT) for visual monitoring of colonized bacteria and responsive antibacterial ability. Specifically, the acidity of the microenvironment caused by bacterial metabolism was applied to drive the color change of bromothymol blue (BTB) on the PPBT membrane from green to yellow, thereby facilitating the early warning of infection and timely treatment. Within 4 h, different concentrations of Staphylococcus aureus (∼105 CFU mL−1), Escherichia coli (∼105 CFU mL−1), Pseudomonas aeruginosa (∼105 CFU mL−1) and Candida albicans (∼104 CFU mL−1) were visually monitored. Moreover, as the local acidity was enhanced via microbial metabolism, ZIF-8 nanoparticles loaded with TCS (TCS@ZIF-8) on the PPBT membrane could release TCS in an acid-responsive manner. At the same time, ROS were generated under 405 nm irradiation to achieve synergistic antibacterial ability. Experiments confirmed that the PPBT membrane has ideal and controllable antibacterial features based on acid responsive release and a synergistic photocatalytic antibacterial mechanism after monitoring. Therefore, the PPBT membrane developed in this work provides a feasible solution for bacterial monitoring and inactivation devices. More importantly, it can be beneficial for meeting the needs of clinical diagnosis and timely treatment of bacterial infection.
研究领域:
生物医学工程
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微生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kai Xu,Xuefeng Zhang,Ming Zhang,Lanhe Zhang,Zicheng Chen
The combination of highly hydrophilic plant fiber and water molecule sensitive material can effectively detect the water content in transformer oil. In this study, two types of rapid response cellulose colorimetric papers (Rp and Bp) were fabricated through a simple impregnation method, with cellulosic fiber serving as the substrate, cresol red/thymol blue as the color developer, boric acid as the pH regulator, and silica aerosol as the filler. Rp and Bp were tested and analyzed using various characterization methods, and their performance in detecting bound water content in transformer oil was investigated. The results indicate that Rp changes from magenta to light yellow and Bp changes from lavender to light yellow with the increase of water content of transformer oil. The water content detection accuracy of both is 92 % and 95 %, respectively. The rapid color change of them is due to boric acid's acidity triggering molecular structure changes in the color developers. After six cycles of use, Rp and Bp still exhibited excellent color-changing properties. Therefore, colorimetric papers have the attributes of rapidity, high efficiency, low cost, and desired accuracy, providing an alternative approach for evaluating transformer oil's water content and showing promising application potential.
研究领域:
材料科学
·
分析化学
·
电气工程
·
高分子科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xun Liu,Shang-Qing Zhang,Xing Wei,Ting Yang,Ming-Li Chen,Jian-Hua Wang
A novel core-shell structure optical pH sensor is developed with upconversion nanoparticles (UCNPs) serving as the core and silica as the shell, followed by grafting bovineserumalbumin (BSA) as another shell via glutaraldehyde cross-linking. The obtained core-shell-shell structure is shortly termed as UCNPs@SiO 2 @BSA, and its surface provides a platform for loading various pH sensitive dyes, which are alike “modules” to make it feasible for measuring pHs within different pH ranges by simply regulating the type of dyes. Generally, a single pH sensitive dye is adopted to respond within a certain pH range. This study employs bromothymol blue (BTB) and rhodamine B (RhB) to facilitate their responses to pH variations within two ranges, i.e., pH 5.99–8.09 and pH 4.98–6.40, respectively, with detection by ratio-fluorescence protocol. The core-shell-shell structure offers superior sensitivity, which is tens of times more sensitive than those achieved by ratio-fluorescence approaches based on various nanostructures , and favorable stability is achieved in high ionic strength medium. In addition, this sensor exhibits superior photostability under continuous excitation at 980 nm. Thanks to the near infrared excitation in the core-shell-shell structure, it effectively avoids the self-fluorescence from biological samples and thus facilitates accurate sensing of pH in various biological sample matrixes .
研究领域:
生物化学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）