NEURON
·
2024
·
IF 14.7
Shunchang Fang,Zhengyi Luo,Zicheng Wei,Yuxin Qin,Jieyan Zheng,Hongyang Zhang,Jianhua Jin,Jiali Li,Chenjian Miao,Shana Yang,Yonglin Li,Zirui Liang,Xiao-Dan Yu,Xiao Min Zhang,Wei Xiong,Hongying Zhu,Wen-Biao Gan,Lianyan Huang,Boxing Li
Recognizing the affective states of social counterparts and responding appropriately fosters successful social interactions. However, little is known about how the affective states are expressed and perceived and how they influence social decisions. Here, we show that male and female mice emit distinct olfactory cues after experiencing distress. These cues activate distinct neural circuits in the piriform cortex (PiC) and evoke sexually dimorphic empathic behaviors in observers. Specifically, the PiC → PrL pathway is activated in female observers, inducing a social preference for the distressed counterpart. Conversely, the PiC → MeA pathway is activated in male observers, evoking excessive self-grooming behaviors. These pathways originate from non-overlapping PiC neuron populations with distinct gene expression signatures regulated by transcription factors and sex hormones. Our study unveils how internal states of social counterparts are processed through sexually dimorphic mechanisms at the molecular, cellular, and circuit levels and offers insights into the neural mechanisms underpinning sex differences in higher brain functions.
研究领域:
神经科学
·
行为科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plant Diversity
·
2020
·
IF 1.864
Xing Huang,Weiqi Li,Xudong Zhang
Exploring approaches to regulate meristem is of special importance and broad interest. In this study, we found that the flavonoid scutellarin, which has a 6-hydroxyl and a 7-glucoside, increased root length through the transcription factor NUTCRACKER (NUC). This root lengthening disappeared in NUC -knockout and reappeared in NUC -rescue plants. Scutellarin induced NUC expression and promoted the division of cortex/endodermal initials. In contrast, naringenin, which has same chemical backbone but without 6-hydroxyl and with 7-hydroxyl group, showed the opposite or no effects. Our results demonstrate that scutellarin promotes root length through NUC-mediated regulatory pathways and reveal that flavonoids with and without the 6-hydroxyl and 7-glucoside have positive and negative effects on meristem size, respectively.
研究领域:
植物生物学
·
分子遗传学
·
发育生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Neuroscience
·
2022
·
IF 5.152
Ting-Ting Pan,Chao Liu,De-Min Li,Tian-Hao Zhang,Wei Zhang,Shi-Lun Zhao,Qi-Xin Zhou,Bin-Bin Nie,Gao-Hong Zhu,Lin Xu,Hua Liu
Animal contextual fear conditioning (CFC) models are the most-studied forms used to explore the neural substances of posttraumatic stress disorder (PTSD). In addition to the well-recognized hippocampal-amygdalar system, the retrosplenial cortex (RSC) is getting more and more attention due to substantial involvement in CFC, but with a poor understanding of the specific roles of its two major constituents-dysgranular (RSCd) and granular (RSCg). The current study sought to identify their roles and underlying brain network mechanisms during the encoding processing of the rat CFC model. Rats with pharmacologically inactivated RSCd, RSCg, and corresponding controls underwent contextual fear conditioning. [ 18 F]-fluorodeoxyglucose positron emission tomography/computed tomography ( 18 F-FDG PET/CT) scanning was performed for each animal. The 5-h and 24-h retrieval were followed to test the formation of contextual memory. Graph theoretic tools were used to identify the brain metabolic network involved in encoding phase, and changes of nodal (brain region) properties linked, respectively, to disturbed RSCd and RSCg were analyzed. Impaired retrieval occurred in disturbed RSCd animals, not in RSCg ones. The RSC, hippocampus (Hip), amygdala (Amy), piriform cortex (Pir), and visual cortex (VC) are hub nodes of the brain-wide network for contextual fear memory encoding in rats. Nodal degree and efficiency of hippocampus and its connectivity with amygdala, Pir, and VC were decreased in rats with disturbed RSCd, while not in those with suppressed RSCg. The RSC plays its role in contextual fear memory encoding mainly relying on its RSCd part, whose condition would influence the activity of the hippocampal-amygdalar system.
研究领域:
神经科学
·
行为神经科学
·
精神病学
·
神经成像
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2022
·Vol. 32
·期 38
·2204794
·
IF 19.924
Han Yang,Zheyan Qian,Jiajia Wang,Jianyou Feng,Chengqiang Tang,Liyuan Wang,Yue Guo,Ziwei Liu,Yiqing Yang,Kailin Zhang,Peining Chen,Xuemei Sun,Huisheng Peng
Electrophysiology and neurochemicals such as Ca 2+ , K + , and Na + on the cerebral cortex can synergistically reflect the neurophysiological states. Transparent electrodes have been reported to record electrocorticography (ECoG) and image Ca 2+ on the cerebral cortex surface. However, Ca 2+ imaging is unable to track extracellular changes correlated with neural activities such as anesthesia, and imaging techniques to monitor K + and Na + are yet unavailable. Here, a flexible multifunctional electrode (FME) based on carbon nanotube array is presented to record ECoG and extracellular ions of Ca 2+ , K + , and Na + . The FME exhibits both lower impedance and higher capacitance than that of conventional gold electrodes. It simultaneously shows stable ion-sensing performance and long-term biocompatibility. The FME realizes multi-model recording of ECoG and extracellular ions on the cerebral cortex surface of rats, providing an effective detection method for brain science.
研究领域:
神经科学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2021
·Vol. 35
·期 6
·e5084
·
IF 1.902
Jian-Sha Li,Hong-mei Qiu,Qing-song Jiang,Yong-hong Liu,Jing Zhang,Tao Gong,Xin-hui Jiang
Emerging evidence suggests that amino acid (AA) neurotransmitters play important roles in the pathophysiological processes of cerebral ischemia. In this work, an HPLC with fluorescence detection (HPLC-FLR) method was developed for the simultaneous determination of 18 AAs in the cortex and plasma after cerebral ischemia in mice. The ischemia model was prepared by bilateral common carotid artery occlusion, and then the cortex and plasma of the sham, ischemia, and naringenin groups were collected. Based on the protein precipitation method, a simple and effective sample preparation method was developed. The treated sample contained minimal proteins and lipids. The analysis of the sample was performed by the proposed HPLC-FLR method in combination with o -phthalaldehyde. The results showed a statistically significant increase in excitatory AAs (aspartic acid and glutamic acid), inhibitory AAs (glycine and 4-aminobutyric acid), phenylalanine, citrulline, isoleucine, and leucine levels, and a decrease of glutathione and phenylalanine levels when compared with the sham group in the cortex. Besides, the administration of naringenin had significant effects on excitatory AAs, inhibitory AA (glycine), glutamine, tyrosine, phenylalanine, and leucine levels when compared with the sham group in the cortex. These findings could be utilized in studying and clarifying the mechanisms of ischemia.
研究领域:
神经科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2017
·
IF 6.395
Tao Fu,Jianxing Huang,Shaobo Lai,Size Zhang,Jun Fang,Jinbao Zhao
The catalytic activity and stability of electrocatalyst is critical for the commercialization of fuel cells, and recent reports reveal the great potential of the hollow structures with Pt skin coat for developing high-powered electrocatalysts due to their highly efficient utilization of the Pt atoms. Here, we provide a novel strategy to prepare the Pt skin coated hollow Ag-Pt structure (Ag-Pt@Pt) of ∼8 nm size at room temperature . As loaded on the graphene, the Ag-Pt@Pt exhibits a remarkable mass activity of 0.864 A/mg Pt (at 0.9 V, vs. reversible hydrogen electrode (RHE)) towards oxygen reduction reaction (ORR), which is 5.30 times of the commercial Pt/C catalyst, and the Ag-Pt@Pt also shows a better stability during the ORR catalytic process. The mechanism of this significant enhancement can be attributed to the higher Pt utilization and the unique Pt on Ag-Pt surface structure, which is confirmed by the density functional theory (DFT) calculations and other characterization methods. In conclusion, this original work offers a low-cost and environment-friendly method to prepare a high active electrocatalyst with cheaper price, and this work also discloses the correlation between surface structures and ORR catalytic activity for the hollow structures with Pt skin coat, which can be instructive for designing novel advanced electrocatalysts for fuel cells.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Brain Structure & Function
·
2024
·Vol. 229
·期 7
·1617-1629
·
IF 2.7
Omholt Stig W.,Lejneva Raissa,Donate Maria Jose Lagartos,Caponio Domenica,Fang Evandro Fei,Kobro-Flatmoen Asgeir
In layer II of the entorhinal cortex, the principal neurons that project to the dentate gyrus and the CA3/2 hippocampal fields markedly express the large glycoprotein reelin (Re + ECLII neurons). In rodents, neurons located at the dorsal extreme of the EC, which border the rhinal fissure, express the highest levels, and the expression gradually decreases at levels successively further away from the rhinal fissure. Here, we test two predictions deducible from the hypothesis that reelin expression is strongly correlated with neuronal metabolic rate. Since the mitochondrial turnover rate serves as a proxy for energy expenditure, the mitophagy rate arguably also qualifies as such. Because messenger RNA of the canonical promitophagic BCL2 and adenovirus E1B 19-kDa-interacting protein 3 (Bnip3) is known to be highly expressed in the EC, we predicted that Bnip3 would be upregulated in Re + ECLII neurons, and that the degree of upregulation would strongly correlate with the expression level of reelin in these neurons. We confirm both predictions, supporting that the energy requirement of Re + ECLII neurons is generally high and that there is a systematic increase in metabolic rate as one moves successively closer to the rhinal fissure. Intriguingly, the systematic variation in energy requirement of the neurons that manifest the observed reelin gradient appears to be consonant with the level of spatial and temporal detail by which they encode information about the external environment.
研究领域:
神经科学
·
分子生物学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Tongfang Xiao,Xianchan Li,Huan Wei,Wenliang Ji,Qingwei Yue,Ping Yu,Lanqun Mao
Spreading depression (SD) is a common pathological process in the brain shown as propagating neuronal depolarization followed by activity depression over the brain, and it is closely related to migraines and epilepsy. Although O2 is known to fluctuate during SD, the difference of O2 responses at different sites in the same brain region remains unknown. In this study, we develop an in vivo electrochemical method with microelectrode arrays (MEAs) to monitor, in real time, O2 fluctuation at multiple sites of rat cortex during SD with high spatial/temporal resolution. Platinum nanoparticles are electrochemically deposited on the multiplexed electrodes of the MEAs to monitor O2 fluctuation simultaneously and selectively via a four-electron reduction process. Configuration of electrode arrays is designed rationally to exclude the probable crosstalk between neighbor recording electrodes during simultaneous measurements. With the MEAs, we find both the basal O2 levels and O2 fluctuations at different sites of the cortex during SD exhibit significant differences, indicating the intensity of energy metabolism and oxidative stress vary at different sites even in the same brain region. Further studies prove that O2 fluctuation is mostly caused by the increase of brain blood flow and the consumption of neuronal O2 during SD. Our study reveals that energy metabolism varies at different sites in brain cortex during SD propagation, which may provide new understanding for SD-related pathological processes.
研究领域:
神经科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MAGNETIC RESONANCE IMAGING
·
2023
·
IF 4.4
Xue Yang,Ying Liu,Cai-xia Fu,Ying-Hua Chu,Qun Chen,He Wang,Da-Xiu Wei,Ye-Feng Yao
Background Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter in human brains, playing a role in the pathogenesis of various psychiatric disorders. Current methods have some non-neglectable shortcomings and noninvasive and accurate detection of GABA in human brains is long-term challenge. Purpose To develop a pulse sequence capable of selectively detecting and quantifying the 1 H signal of GABA in human brains based on optimal controlled spin singlet order. Study Type Prospective. Subjects/Phantom A phantom of GABA (pH = 7.3 ± 0.1) and 11 healthy subjects (5 females and 6 males, body mass index: 21 ± 3 kg/m 2 , age: 25 ± 4 years). Field Strength/Sequence 7 Tesla, 3 Tesla, GABA-targeted magnetic resonance spectroscopy (GABA-MRS-7 T, GABA-MRS-3 T), magnetization prepared two rapid acquisition gradient echoes sequence. Assessment By using the developed pulse sequences applied on the phantom and healthy subjects, the signals of GABA were successfully selectively probed. Quantification of the signals yields the concentration of GABA in the dorsal anterior cingulate cortex (dACC) in human brains. Statistical Tests Frequency. Results The 1 H signals of GABA in the phantom and in the human brains of healthy subjects were successfully detected. The concentration of GABA in the dACC of human brains was 3.3 ± 1.5 mM. Data Conclusion The developed pulse sequences can be used to selectively probe the 1 H MR signals of GABA in human brains in vivo. Evidence Level 1 Technical Efficacy Stage 1
研究领域:
医学影像学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROTOXICOLOGY AND TERATOLOGY
·
2025
·
IF 2.8
Fangfei Li,Jie Zhang,Xiaoli Ma,Hao Chen,Guiqiang Liang,Yunfeng Zou
Excessive accumulation of manganese (Mn) can cause neuroinflammation, impairing cognitive function. SPON1, a secreted glycoprotein in the extracellular matrix, is implicated in neuroinflammation, but its role in activating pro-inflammatory pathways in Mn-induced neuroinflammation remains unclear. This study employed in vivo and in vitro models to investigate Mn neuroinflammation. The expression levels of SPON1 and the ERK1/2/NF-κB pathway associated with inflammation were measured in male C57BL/6 mice after gavage of Mn at different doses (0, 25, 50, 100 mg/kg) for 12 weeks. SPON1 levels were measured after primary hippocampal neurons, primary cortical neurons, neuroblastoma cells (N2a), and microglial cells (BV2) were exposed to various concentrations of Mn for 24 h. We observed that in vivo Mn exposure significantly decreased SPON1 expression in the cortex but not in the hippocampus. Similarly, in vitro experiments demonstrated that Mn exposure significantly reduced SPON1 levels in primary cortical neurons, N2a, and BV2. In addition, Mn exposure increased the expression levels of ERK1/2 and NF-κB pathway proteins in the mouse cortex. Because BV2 cells are susceptible to inflammatory signals, they were chosen to elucidate how SPON1 induces neuroinflammation during Mn exposure. SPON1 knockdown increases the expression of inflammatory factors, whereas SPON1 overexpression inhibits the activation of the ERK1/2/NF-κB pathway and reduces inflammatory factor levels. In summary, these results suggest that Mn may affect the activation of ERK1/2/NF-κB pathway and the expression of inflammatory factors by inhibiting SPON1, ultimately promoting neuroinflammation.
研究领域:
神经科学
·
毒理学
·
分子生物学
·
免疫学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2021
·
IF 9.501
Lihua Zhao,Zhiyu Liu,Faizal Soyekwo,Changkun Liu,Yunxia Hu,Qingshan Jason Niu
Sludge thickening from sewage treatment plants is an essential step in reducing the sludge volume and processing cost of the subsequent treatment. Forward osmosis (FO) technology is one of the research endeavors used in the field of membrane separation and feed solution concentration. However, the traditional used single-skinned FO membranes encounter the problems of serious internal concentration polarization (ICP) and fouling phenomenon within porous substrates . A novel double-skinned FO membrane was proposed in this study in order to enhance the anti-fouling performance and mitigate the ICP phenomenon. Single-skinned FO membrane using the same substrate was also fabricated. Results demonstrated that the double-skinned FO membrane significantly reduced ICP and exhibited higher flux when using draw solutions with high concentrations as compared to the single-skinned FO membrane. Furthermore, the double-skinned FO membrane demonstrated reasonably high-water flux (11.6 LMH), whereas zero flux was recorded from the single-skinned FO membrane when using the feed solution that contained 15.0 g/L organic foulants in the fouling tests. At last, the double-skinned FO membrane exhibited superior anti-fouling properties and much higher flux than that of single-skinned FO membrane operated under both modes during the long-term sludge thickening process, indicating the great potential of the double-skinned FO membrane to deal with the high-water-content sewage sludge in the water treatment process.
研究领域:
环境工程
·
膜科学与技术
·
水处理
·
分离科学
·
环境功能材料
✓ 已验证
DOI
高影响因子
PeerJ
·
2022
·
IF 3.061
Wentao Su,Miao Zhang,Wenbo Wei,Haitao Wang,Wei Zhang,Zhongyu Li,Mingqian Tan,Zongzheng Chen
During electrospinning, the fibers deposited on the collector are usually randomly oriented in a disordered form. Researchers hope to generate periodic structures to expand the application of electrospinning, including improving the sensing properties of electronic and photonic devices, improving the mechanical properties of solid polymer composites and directional growth of human tissues. Here, we propose a technique to control the preparation of aligned foodborne nanofibers by placing dielectric polymers on microfluidic devices, which does not require the use of metal collectors. This study was conducted by introduced PEDOT:PSS polymer as a ground collector to prepare aligned foodborne nanofibers directly on the microfluidic platform. The fluidity of the electrolytic polymer collector makes it possible to shape the grounding collector according to the shape of the microcavity, thus forming a space adjustable nanofiber membrane with a controllable body. The simplicity of dismantling the collector also enables it extremely simple to obtain a complete electrospun fiber membrane without any additional steps. In addition, nanofibers can be easily stacked into a multi-layer structure with controllable hierarchical structures. The Caco-2 cells that grow on the device formed a compact intestinal epithelial layer that continuously expresses the tightly bound protein ZO-1. This intestinal barrier, which selectively filters small molecules, has a higher level of TEER, reproducing intestinal filtration functions similar to those of in vivo models. This method provides new opportunities for the design and manufacture of various tissue scaffolds, photonic and electronic sensors.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）