Journal of Cosmetic Dermatology
·
2025
·Vol. 24
·期 7
·e70218
·
IF 2.5
Xue-dong Bai,Yu-chen Liu,Sai-ya Ge,Wei-cheng Fei
Background Proteoglycans (PGs) derived from salmon nasal cartilage are believed to have antiaging effects on the skin. However, comprehensive evaluations of their impact on various skin parameters in Chinese populations remain limited. Aims This study aims to evaluate the efficacy of oral PG supplementation in enhancing skin elasticity, hydration, and reducing roughness, wrinkles, and pigmentation in healthy adult volunteers. Methods A 56-day randomized, double-blind, placebo-controlled trial was conducted involving 66 subjects aged 30–60. Subjects received a daily dose of 20 mg PG, and skin parameters were measured at baseline, 28 days, and 56 days. The study assessed skin elasticity, hydration, roughness, wrinkles, melanin content, and brown spots while monitoring for any adverse effects. Results Subjects receiving PG supplementation showed significant improvements in skin elasticity and hydration at both 28 days and 56 days ( p < 0.001), with reductions in skin roughness and wrinkles ( p < 0.001), and a significant decrease in melanin content and brown spots ( p < 0.001). Compared to the placebo group, the PG group exhibited significant improvements in most skin parameters by 56 days, except in the wrinkle area percentage at the crow's feet, where no significant difference was observed. PG was well tolerated, with no adverse effects reported. Conclusions Our findings suggest that daily oral intake of 20 mg PG effectively improves skin health by enhancing elasticity, hydration, and reducing signs of aging such as wrinkles and pigmentation.
研究领域:
生物化学
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皮肤病学
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营养学
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临床试验
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2023
·
IF 18.9
Huijuan Wang,Deshun Sun,Weiming Lin,Chao Fang,Kui Cheng,Zhengzhou Pan,Daping Wang,Zhangfa Song,Xiaojun Long
Full-thickness skin wounds are have continued to be reconstructive challenges in dermal and skin appendage regeneration, and skin substitutes are promising tools for addressing these reconstructive procedures. Herein, the one-step fabrication of a cell sheet integrated with a biomimetic hydrogel as a tissue engineered skin for skin wound healing generated in one step is introduced. Briefly, cell sheets with rich extracellular matrix, high cell density, and good cell connections were integrated with biomimetic hydrogel to fabricate gel + human skin fibroblasts (HSFs) sheets and gel + human umbilical vein endothelial cells (HUVECs) sheets in one step for assembly as a cell sheet-laden hydrogel (CSH). The designed biomimetic hydrogel formed with UV crosslinking and ionic crosslinking exhibited unique properties due to the photo-generated aldehyde groups, which were suitable for integrating into the cell sheet, and ionic crosslinking reduced the adhesive force toward the substrate. These properties allowed the gel + cell sheet film to be easily released from the substrate. The cells in the harvested cell sheet maintained excellent viability, proliferation, and definite migration abilities inside the hydrogel. Moreover, the CSH was implanted into a full-thickness skin defects to construct a required dermal matrix and cell microenvironment. The wound closure rate reached 60.00 ± 6.26% on the 2nd day, accelerating mature granulation and dermis formation with skin appendages after 14 days. This project can provide distinct guidance and strategies for the complete repair and regeneration of full-thickness skin defects, and provides a material with great potential for tissue regeneration in clinical applications.
研究领域:
组织工程学
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再生医学
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
De Bai,Haowei Chen,Lijuan Liu,Nanxi Xiang,Jichuan Zhang,Chengyu Wu,Jiaheng Zhang,Fang Wang
Pterostilbene (PTB) is a hydrophobic compound derived from plants in the Astragalus family. PTB possesses remarkable skin brightening and antioxidant properties. However, delivery methods for PTB require enhancement, and this can be achieved by formulating suitable carriers. In this study, we prepared ionic liquid microcapsules containing PTB by using a microencapsulation technique based on a matrine and cocinic acid ([Mat][Coc]) ionic liquid. The particle size of the microcapsules was 154.5 ± 3.42 nm, and the encapsulation efficiency was as high as 99.7 %. Further, the PTB-loaded ionic liquid microcapsules had good biocompatibility, enhanced skin retention (7.7-times higher than that of free PTB), and improved skin penetration without damaging the skin structure. Clinical testing with human subjects revealed that the microcapsule emulsion reduced fine lines and brightened skin tone. Therefore, PTB-loaded ionic liquid microcapsules have promise for use in cosmetic formulations and applications in transdermal drug delivery and efficacy enhancement.
研究领域:
药剂学
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化妆品科学
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药物递送系统
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Qi Tian,Peng Quan,Liang Fang,Hui Xu,Chao Liu
A transdermal/topical absorption classification system for the characterization of the systemic or local delivery of drugs is the theoretical basis for the design and evaluation of transdermal/topical formulations. A classification system was established on the basis of the in vitro and in vivo skin permeation/retention behaviors of 12 model drugs. Drug skin penetration/retention exhibited a significant correlation with physicochemical parameters (log K O/W , molecular weight, polar surface area, and polarizability). Four representative model drugs were selected to clarify the molecular mechanisms of drug skin permeation/retention behaviors. The excellent lipid-disrupting effect and enhanced partitioning exhibited by propranolol (high permeation–high retention) and zolmitriptan (high permeation-low retention) via the formation of moderate H-bonds with skin lipids were proven by ATR–FTIR (Δ ν asCH2 > 2 cm −1 ), Raman spectra (ΔLPP, SPP > 0.2 nm), and X-ray scattering (lipid crystallization) and were supported by 13 C NMR results. The low lipid miscibility of zolmitriptan (Δ H zolmitriptan-lipid = 126.92 J/g) caused the low skin retention of this drug. High polarizabiltiy ( α = 38.5 × 10 −24 cm 3 ) and low H-bond forming capability ( E H-bond = 0 kcal/mol) restricted terbinafine (low permeation–high retention) in terms of partitioning ( k D-SC = 0.09). Diclofenac (low permeation–low retention) stabilized skin lipids through the formation of strong H-bonds and exhibited excessive drug–lipid miscibility (Δ H diclofenac-skin = −128.73 J/g), thus restricting its skin absorption. This classification system reflects the most essential drug skin absorption characteristics and provides a theoretical basis for the design of transdermal/topical formulations.
研究领域:
药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 2.997
Ding Zhang,Wei Wang,Tao Hou,Yanjun Pang,Chao Wang,Shuai Wu,Qing Wang
Cutaneous melanoma is the deadliest form of skin cancer, and gambogic acid (GA) exhibits potent anti-melanoma activity. However, clinical application of GA via intravenous injection and oral administration is limited by systemic toxicity and rapid metabolism in the blood. Here, we developed a new, topical route of GA delivery for anti-melanoma activity and reduction of systemic toxicity. The results indicated that the barrier of the stratum corneum (SC) and low diffusion of GA in the hydrophilic viable skin (epidermis and dermis) limited the GA penetration through intact skin. The combination of azone (AZ) and propylene glycol (PG) showed obvious synergistic effects on skin penetration by GA via improving the permeability of the SC and greatly increasing the skin accumulation of GA, thereby forming a high drug concentration in the skin and achieving a topical targeted treatment of melanoma . In addition, GA (AZ–PG) achieved the same anti-melanoma effect via topical delivery as via intravenous injection. Intravenous injection and oral administration of GA induced remarkable pathological changes in various organs in mice, whereas GA was not toxic to various organs or to the skin via topical delivery. These findings indicated that topical administration of GA is an alternative route for melanoma treatment.
研究领域:
肿瘤学
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药理学
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皮肤病学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
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IF 8.8
Xiaoqian Li,Na Zheng,Wenhui Zhang,Siyu Sun,Qirui An,Zimeng Li,Yining Ji,Sujing Wang
Contaminants present in dust adhering to the skin can pose a significant risk to human health through dermal absorption and hand-to-mouth contact. The adhesion capacity of dust differs significantly from that of soil due to its physicochemical properties . Therefore, applying the raw soil exposure parameters to estimate the health risks associated with dermal exposure to dust may lead to erroneous conclusions. In this study, we quantified the maximum amount of dust that adhered to the skin (MAD max ) and the upper limit of dust-skin adherence factor (DSAF max ) in 26 adults using element markers as a proxy for dust. The volunteers were exposed to dust and rinse water samples were collected from their hands, forearms, lower legs, and feet. We analyzed both the raw dust samples and the rinse water samples for 11 element markers, including Be, V, Cr, Mn, Co, Ni, Cu, Zn, Se, Ba, and Pb. The results showed that the MAD max of indoor dust and outdoor dust increased by 0.08–0.62 mg and 0.33–0.56 mg following a 1 cm 2 increase in skin surface area, respectively. Based on best dust element markers, the body part-weighted dust-skin adherences (WDSAF max s) of indoor dust and outdoor dust were 0.35 and 0.64 mg/cm 2 , respectively. A smaller particle size and higher moisture content resulted in a larger DSAF max . Only when indoor dust concentrations exceed 24.2 mg/m 3 or outdoor dust concentrations exceed 44.3 mg/m 3 , can the WDSAF max be applied directly in the health risk assessment of dermal exposure to dust. The method from this study can be re-applied in different regions, and the adherence data can help to improve future studies on the health effects of dermal exposure to dust.
研究领域:
环境科学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 36
·1-12
·
IF 2.8
Chen Yan,Tian Mi,Song Shiqiang,Wu Min,Li Weizhen,Gan Wenjun
Electronic skin, represented by stretchable flexible strain sensors, is widely used to detect the movement of human joints, etc., because of its comfortable attachment to the skin. The strain sensors with adhesive properties are essential in terms of conformal attachment to skin and transmitting signals precisely. However, it is of great importance to develop flexible sensors with reversible attachment and detachment in practical applications. In this work, a novel skin temperature-triggering adhesive conductive composite was obtained by copolymerization of acrylate monomers stearyl acrylate (SA) and tetradecyl acrylate (TA) in situ with vinylsilane grafted carbon nanotubes (VCNTs) and carbon black (CB). The obtained composite has the characteristics of adjustable stiffness and adhesion by changing temperature due to the crystallizable alkyl side chain of SA and TA. Specifically, the composite exhibits adhesion at skin temperature and loses its adhesive properties at lower temperature due to the amorphous-to-semicrystalline phase transition of copolymer matrix. While the coordination of zero-dimensional (0D) CB and one-dimensional (1D) VCNTs imparts the composite good electrical conductivity of 3.6 × 10 6 Ω cm. The composite-based strain sensor displays stable monitor results of human motion. Therefore, this work might enrich the preparation strategies for flexible sensors in wearable electronic devices and electronic skin.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
CURRENT ISSUES IN MOLECULAR BIOLOGY
·
2024
·Vol. 46
·期 9
·9916-9927
·
IF 2.8
Miao Tian,Yuan-Yuan Xu,Yang-Nan Li,Shen Yu,Yi-Lin Wang,Xiao-Lai Ma,Ye-Wang Zhang
Dermatan sulfate and chondroitin sulfate are dietary supplements that can be utilized as prophylactics against thrombus formation. Low-molecular-weight dermatan sulfate (LMWDS) is particularly advantageous due to its high absorbability. The enzymatic synthesis of low-molecular-weight dermatan sulfates (LMWDSs) using chondroitin B lyase is a sustainable and environmentally friendly approach to manufacturing. However, the industrial application of chondroitin B lyases is severely hampered by their low catalytic activity. To improve the activity, a semi-rational design strategy of engineering the substrate-binding domain of chondroitin B lyase was performed based on the structure. The binding domain was subjected to screening of critical residues for modification using multiple sequence alignments and molecular docking. A total of thirteen single-point mutants were constructed and analyzed to assess their catalytic characteristics. Out of these, S90T, N103C, H134Y, and R159K exhibited noteworthy enhancements in activity. This study also examined combinatorial mutagenesis and found that the mutant H134Y/R159K exhibited a substantially enhanced catalytic activity of 1266.74 U/mg, which was 3.21-fold that of the wild-type one. Molecular docking revealed that the enhanced activity of the mutant could be attributed to the formation of new hydrogen bonds and hydrophobic interactions with the substrate as well as neighbor residues. The highly active mutant would benefit the utilization of chondroitin B lyase in pharmaceuticals and functional foods.
研究领域:
生物工程
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酶学
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医药生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Jingyi Liu,Jingwen Yu,Huiling Chen,Yaping Zou,Yuxiang Wang,Chen Zhou,Lei Tong,Peilei Wang,Tangjinhai Liu,Jie Liang,Yong Sun,Xingdong Zhang,Yujiang Fan
The skin has a multilayered structure, and deep-seated injuries are exposed to external microbial invasion and in vivo microenvironmental destabilization. Here, a bilayer bionic skin scaffold (Bilayer SF) was developed based on methacrylated sericin protein to mimic the skin’s multilayered structure and corresponding functions. The outer layer (SF@TA), which mimics the epidermal layer, was endowed with the function of resisting external bacterial and microbial invasion using a small pore structure and bio-crosslinking with tannic acid (TA). The inner layer (SF@DA@Gel), which mimics the dermal layer, was used to promote cellular growth using a large pore structure and introducing dopamine (DA) to regulate the wound microenvironment. This Bilayer SF showed good mechanical properties and structural stability, satisfactory antioxidant and promote cell proliferation and migration abilities. In vitro studies confirmed the antimicrobial properties of the outer layer and the pro-angiogenic ability of the inner layer. In vivo animal studies demonstrated that the bilayer scaffolds promoted collagen deposition, neovascularization , and marginal hair follicle formation, which might be a promising new bionic skin scaffold.
研究领域:
生物材料学
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组织工程学
·
再生医学
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产品关联
DOI
PubMed
高影响因子
SKIN RESEARCH AND TECHNOLOGY
·
2023
·Vol. 29
·期 11
·e13465
·
IF 2.2
Xi-Liang Zang,Fei Gao,Zhao Zhang,Lin-Hua Shen,Yue-Hai Pan
Objective We aimed to develop an electroactive antibacterial material for the treatment of skin wound diseases. Method To this aim, we modified chitosan (CS), a biocompatible polymer, by coupling it with graphene (rGO) and an antimicrobial polypeptide DOPA-PonG1. The material's effect on skin injury healing was studied in combination with external electrical stimulation (EEM). The structure, surface composition, and hydrophilicity of the modified CS materials were evaluated using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and contact angle measurements. We studied NIH3T3 cells cultured with modified materials and subjected to EEM to assess viability, adhesion, and tissue repair-related gene expression. Results SEM data demonstrated that rGO was distributed uniformly on the surface of the CS material, increasing surface roughness, and antimicrobial peptides had minimal impact on surface morphology. FTIR confirmed the uniform distribution of rGO and antibacterial peptides on the material surface. Both rGO and DOPA-PonG1 enhanced the hydrophilicity of CS materials, with rGO also improving tensile strength. The dual modification of CS with rGO and DOPA-PonG1 synergistically increased antibacterial efficacy. Cellular events and gene expression relevant to tissue repair process were enhanced by these modifications. Furthermore, EEM accelerated epidermal regeneration more than the material alone. In a rat skin wound model, DOPA-PonG1@CS/rGO dressing combined with electrical stimulation exhibited accelerated healing of skin defect. Conclusion Overall, our results demonstrate that CS materials modified with rGO and DOPA-PonG1 have increased hydrophilicity, antibacterial characteristics, and tissue regeneration capacities. This modified material in conjunction with EEM hold promise for the clinical management for dermal wounds.
研究领域:
生物医学工程
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材料科学
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组织工程
·
伤口愈合
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Shaoquan Bian,Xiaohua Hu,Hao Zhu,Weili Du,Chenmin Wang,Liangliang Wang,Liuzhi Hao,Yuming Xiang,Fengzhen Meng,Chengwei Hu,Zhiyun Wu,Jing Wang,Xiaohua Pan,Min Guan,William Weijia Lu,Xiaoli Zhao
Three-dimensional (3D) bioprinting has advantages for constructing artificial skin tissues in replicating the structures and functions of native skin. Although many studies have presented improved effect of printing skin substitutes in wound healing, using hydrogel inks to fabricate 3D bioprinting architectures with complicated structures, mimicking mechanical properties, and appropriate cellular environments is still challenging. Inspired by collagen nanofibers withstanding stress and regulating cell behavior, a patterned nanofibrous film was introduced to the printed hydrogel scaffold to fabricate a composite artificial skin substitute (CASS). The artificial dermis was printed using gelatin–hyaluronan hybrid hydrogels containing human dermal fibroblasts with gradient porosity and integrated with patterned nanofibrous films simultaneously, while the artificial epidermis was formed by seeding human keratinocytes upon the dermis. The collagen-mimicking nanofibrous film effectively improved the tensile strength and fracture resistance of the CASS, making it sewable for firm implantation into skin defects. Meanwhile, the patterned nanofibrous film also provided the biological cues to guide cell behavior. Consequently, CASS could effectively accelerate the regeneration of large-area skin defects in mouse and pig models by promoting re-epithelialization and collagen deposition. This research developed an effective strategy to prepare composite bioprinting architectures for enhancing mechanical property and regulating cell behavior, and CASS could be a promising skin substitute for treating large-area skin defects.
研究领域:
生物医学工程
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组织工程
·
材料科学
·
再生医学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
·
2014
·
IF 0.491
Hengfeng Fan,Huafeng Zhou,Chaolong Ma,Yiqing Huang,Yan Li,Qiang Xia
The nanostructured lipid carrier (NLC) encapsulating two potent skin whitening ingredients was developed by our group for cosmetic and dermatological treatment. The particle showed spherical shape morphology under atomic force microscope, and the value of the zeta potential of the NLC was − 65.4 ± 4.3 mV. Good physical stability was observed by dynamic light scattering during 45 days’ storage, respectively. The skin whitening effect was improved when applying the NLC. Therefore, the NLC might be a potential delivery vehicle in dermal products.
研究领域:
化妆品学
·
皮肤病学
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）