JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
Youyun Liu,Junxi Liu,Rui Xu,Yonglei Yuan,Qiwen Sun,Susu Ding,Feifei Wang,Liping Qu
Ethnopharmacological relevance Ludwigia adscendens (L.) Hara ( L. adscendens ) is a Traditional Chinese Medicine (TCM), first mentioned in Sheng Cao Yao Xing Bei Yao ( Essential Guide to Raw Herbal Properties ). It clears heat, removes dampness, detoxifies, and reduces swelling. In TCM, it is prescribed for acne caused by dampness-heat accumulation. It is also used for cold, fever, sore throat, boils, dermatitis, and snake or dog bites. Research shows it has antioxidant, antibacterial, anti-inflammatory, and anti-lipoxygenase effects. However, studies on its impact on sebaceous gland function (a key factor in acne pathogenesis) are still limited. Aim of the study This study aims to investigate the effects of L. adscendens extract (LHE) on sebaceous gland function and its mechanisms. Materials and methods Active compounds in LHE were identified using high-performance liquid chromatography (HPLC). Its effects on lipid synthesis, sebocyte proliferation, and reactive oxygen species (ROS) were assessed in a 5α-dihydrotestosterone (DHT)-induced sebocyte model. Untargeted lipidomics was used to analyse sebum composition and detect LHE-induced changes. Anti-inflammatory and antioxidant activities were evaluated using zebrafish models and biochemical assays. Sebum-regulatory efficacy was measured to assess LHE's impact on sebum production. Results LHE showed strong biological activity by activating the PPARγ/LXRα/SREBP1 pathway, reducing lipid production, inhibiting sebocyte hyperproliferation, and delivering notable antioxidant and anti-inflammatory effects. Lipidomic analysis revealed that LHE effectively altered lipid composition, especially by lowering triglycerides (TG), diacylglycerols (DG), ceramides (Cer), and hexosyl-1-ceramides (Hex1Cer), with major changes observed in medium- and long-chain TG and phosphatidylethanolamine (PE) levels. Efficacy tests confirmed that LHE reduced sebum production and improved skin texture by shrinking pore size. Conclusions LHE improves sebaceous gland function by reducing lipid overproduction and hyperproliferation through the PPARγ/LXRα/SREBP1 pathway. It also enhances the lipid profile by lowering key lipid molecules like TGs and PEs. Moreover, LHE shows anti-inflammatory and antioxidant effects. These findings suggest its therapeutic potential for sebaceous gland-related conditions and reveal new insights into its mechanism in topical use.
研究领域:
药理学
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民族药理学
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皮肤科学
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分子生物学
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生物化学
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脂质代谢
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植物学
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产品关联
DOI
PubMed
高影响因子
FIBERS AND POLYMERS
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2016
·Vol. 17
·期 2
·305-309
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IF 1.022
Yu Yalun,Cai Muyuan,Zhou Lan,Shao Jianzhong
The accumulation and autoxidation of residual oily soil from human sebum is regarded as a major reason for the aging and yellowing of clothing and household textiles. In this study, the yellowing degree of cotton fabrics soiled with human sebum components and aged under various environmental conditions (various temperatures, humidities and light intensities etc.) were investigated. The representative sebum components including olive oil, squalene, oleic acid and cottonseed oil were selected and their chemical changes were studied by FTIR analysis. The results showed that, olive oil, squalene, oleic acid and cottonseed oil could caused more significant fabric-yellowing than other components in human sebum, and the yellowing degree could become higher with increasing temperatures, humidities and/or light intensities of aging environments. The chemical structures of olive oil, squalene and oleic acid changed remarkably after aging treatment. The aging products contained hydroxyl groups and carbonyl groups, and the conjugated C=O group was considered to be the chemical species leading to the yellowing of sebum-soiled fabrics.
研究领域:
纺织科学
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生物化学
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DOI
高影响因子
Natural Product Communications
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2022
·
IF 1.496
Yuyan Zhu,Lixia Zhang,Xiyuan Zhou
The prevalence of acne vulgaris is high, but the topical retinoids used as the foundation of treatment have teratogenic and photosensitivity properties. Previous studies have suggested that honokiol, a small-molecule compound extracted from Magnolia officinalis, could effectively inhibit Cutibacterium acnes (C acnes) and inflammation in vitro. However, the effect in vivo is unclear. The rabbit ear acne model that we created showed obvious comedones and hyperkeratosis. These lesions were repeatedly measured and recorded by dermatoscopy (ultraviolet light). Compared with the control group, topical 2.5% honokiol cream obviously improved the comedones and hyperkeratosis and effectively reduced sebum secretion, as shown by Oil Red O staining. The effects were equivalent to those of adapalene gel without local side effects. We added honokiol's other functions to acne treatment in addition to antiinflammation, but further studies are needed.
研究领域:
皮肤病学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
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2019
·
IF 3.618
Xiao-Jin Pei,Nan Chen,Yu Bai,Jian-Wen Qiao,Sheng Li,Yong-Liang Fan,Tong-Xian Liu
Insect cuticular hydrocarbons (CHCs), the evolutionary products of aquatic hexapod ancestors expanding to terrestrial environment, are deposited on the surface of insect integument and originally functioned primarily as waterproofing agents. CHCs are derived from the conserved fatty acid synthesis pathway in insects. However, the pivotal fatty acid synthase (FAS) involved in hydrocarbon (HC) biosynthesis remains unknown in many insect orders including the primitive Blattodea . Here, we investigated functional FAS genes that modulate cuticular lipid biogenesis in the German cockroach , Blattella germanica (L.). Based on our full-length transcriptomic data and the available genomic data, seven FAS genes ( BgFas1–7 ) were identified from B. germanica . Tissue-specific expression analysis revealed that BgFas1 , BgFas3 , BgFas4 and BgFas7 were highly expressed in the integument, whereas BgFas2 was dominantly expressed in the fat body. BgFas5 /6 mRNA was almost negligible in the tested tissues. Systemic RNAi screen was performed against BgFas1–7 , we found that only RNAi knockdown of BgFas1 caused a dramatic reduction of methyl-branched HCs (mbHCs) and a slight decrease of straight-chain HCs (scHCs) for both internal and external HCs. Significant reduction of cuticular free fatty acids (cFFAs) was also detected within BgFas1 -repressed cockroaches, while repression of CYP4G19 resulted in dramatic increase of cFFAs. Moreover, we found that BgFas1 mRNA levels were correlated with insect molting cycles, and could be induced by long-term mild dryness treatment. Furthermore, desiccation assay revealed that BgFas1 suppression accelerated water loss and led to early death of cockroaches under desiccation. Our results indicate that BgFas1 is necessary for both HC and cFFA biosynthesis in B. germanica . In addition, our study also confirms that cuticular lipids, particularly mbCHCs, are critical for desiccation resistance in B. germanica .
研究领域:
昆虫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
·
IF 6.7
Lerong Qi,Xiangzi Li,Yachen Ren,Tingxiang Yang,Yang Guan,Dong Hao Wang,Zhen Wang
Sebum fatty acids and squalene are major components of skin secretions and play a critical role in the pathogenesis of dermatological conditions through their compositional alterations. Human sebum fatty acids are uniquely diverse in their chain length, double bond positions, and chain branching; squalene is a rare component of skin secretions with six double bonds. We introduce a facile workflow for the analysis of sebum fatty acids and squalene for elucidating their contribution to the state of skin health or disease using advanced mass spectrometric methods for qualitative and quantitative analyses. Sebum from dermatologically healthy individuals was extracted, and covalent adduct chemical ionization (CACI), electron ionization (EI), and Paternò–Büchi (PB) tandem mass spectrometry (MS/MS) techniques were employed for the identification of fatty acids with varying methyl branch and/or carbon–carbon double bond positions. A method for the full structural characterization of squalene via CACI tandem mass spectrometry was also developed. In sebum, we report for the first time unusual straight-chain polyunsaturated fatty acid isomers (n18:2n-7, n18:2n-3, n20:2n-12, and n20:2n-7), a series of previously unreported branched-chain saturated fatty acids with C19–C26, and a novel branched-chain monounsaturated fatty acid i15:1n-9. A total of 64 fatty acids were identified, exceeding the previous reports. Our study established a comprehensive qualitative and quantitative workflow for the analysis of sebum fatty acids and squalene, with the potential to advance dermatological research and clinical diagnostics.
研究领域:
皮肤病学
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脂质组学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
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2023
·
IF 8.9
Mingpeng Yang,Nan Sun,Xiaochen Lai,Jiamin Wu,Lifan Wu,Xingqiang Zhao,Lihang Feng
Wearable sweat sensors, a product of the development of flexible electronics and microfluidic technologies, can continuously and noninvasively monitor abundant biomarkers in human sweat; however, sweat interferences, such as sebum, can reduce sensor reliability and accuracy. Herein, for the first time, the influence of sebum on the potentiometric response of an all-solid-state pH sensor was studied, and the obtained experimental results show that sebum mixed in sweat can decrease the potential response of the sensor and the slope of its calibration curve. A paper-based sandwich-structured pH sensor that can filter the sebum mixed in sweat was proposed based on commonly used oil-control sheets. Moreover, the hydrophilic properties, microstructure, and microfluidic performance of the sensor were investigated. The detection performance of the paper-based sandwich-structured pH sensor was comprehensively evaluated in terms of calibration in the presence of sebum and potentiometric response upon the addition of sebum. Furthermore, the anti-interference ability of the sensor was evaluated using different analytes under various deformation conditions. On-body trials were conducted to verify the performance, and their results showed that the proposed sensor can filter over 90% of the sebum in sweat, significantly enhancing sensor reliability and accuracy. Additionally, microfluidic channels could be simply fabricated using a scissor and paper, obviating the need for complex micromachining processes, such as photolithography and laser engraving. Overall, this work illustrates the influence of sebum on the detection performance of traditional potentiometric wearable sensors and paves the way for their development for real-world applications.
研究领域:
生物医学工程
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柔性电子
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传感器技术
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产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
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2022
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IF 6.206
Yubo Liu,Weiming Sun,Kai Feng,Yang Wu,Bo Yu,Shujuan Liu,Feng Zhou
In recent years, oil-contained deicing materials have aroused peoples' concern due to their weak ice adhesion feature. Yet the loss of lubricant on oil-contained surface caused by contact or volatilization should be considered. Oily sebum secreted by sebaceous glands lubricates and moisturizes our skin, and can be reproduced continuously even after daily cleaning. In this work, inspired by sebaceous glands, a self-lubricant (SL) coating with ultra-low ice adhesion and excellent deicing durability was designed via continuously secreting lubricants. To determine the suitable simulant of oily sebum, the tribological tests and theoretical simulation analysis of lubricant on ice were conducted, confirming the effect of polydimethylsiloxane (PDMS) lubricants. By well mixing lubricant and resin, and sebaceous glands-like structures formed in coating matrix after curing, which endowed coating with lubricant storage feature and sebum-like secretion structure. The affinity of PDMS molecules to ice and the regeneration of lubricant film ensured the deicing durability of coating. Ice adhesion strength can be kept below 100 kPa even after 100 icing-deicing cycles, and can recover low ice adhesion strength (~50 kPa) via lubricant secreting. This work highlights the importance of lubricant selection and gives a new vision to designing durable icephobic materials.
研究领域:
材料科学
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表面工程
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涂层技术
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产品关联
DOI
高影响因子
Materials Horizons
·
2024
·
IF 12.2
Hao Liu,Zhiming Liu,Hao Zhang,Ke Huang,Xiaohui Liu,Hui Jiang,Xuemei Wang
The non-homeostasis of sebum secretion by the sebaceous glands in a complicated microenvironment dramatically impacts the skin health of many people in the world. However, the complexity and hydrophobicity of sebum mean a lack of diagnostic tools, which makes it challenging to determine the reason behind cortical imbalances. Herein, a biomimetic mineralized aggregates (PTL@Au and PTB@Au) strategy has been proposed, which could obtain molecular information about sebum by surface-enhanced Raman spectroscopy (SERS). The breaking of disulfide bonds leads to changes in hydrogen bonding, which transform the natural protein into amyloid-like phase transition protein with β-sheets. It provides sites for the nucleation and crystallization of gold nanocrystals to build mineralized aggregates. The mineralized aggregates show robust adhesion stability at the interfaces of different materials through hydrogen bonding and electrostatic interactions. The stabilization, hydrophobicity (contact angle: 134°), and optical transmission (75%) of the structure could result in superior SERS performance for sebum analysis. It should be noted that this enables the sebum detection of clinical samples while ensuring safety. Such generalized bionic mineralization construction and diagnosis methods also serve as an advanced paradigm for a range of biomedical applications.
研究领域:
皮肤病学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2022
·
IF 4.132
Wei Fu,Linxin Xu,Qiwen Yu,Jiajia Fang,Guohua Zhao,Yi Li,Chenying Pan,Hao Dong,Di Wang,Haiyan Ren,Yi Guo,Qingjun Liu,Jun Liu,Xing Chen
Background: Currently, Parkinson’s disease (PD) diagnosis is mainly based on medical history and physical examination, and there is no objective and consistent basis. By the time of diagnosis, the disease would have progressed to the middle and late stages. Pilot studies have shown that a unique smell was present in the skin sebum of PD patients. This increases the possibility of a noninvasive diagnosis of PD using an odor profile. Methods: Fast gas chromatography (GC) combined with a surface acoustic wave sensor with embedded machine learning (ML) algorithms was proposed to establish an artificial intelligent olfactory (AIO) system for the diagnosis of Parkinson’s through smell. Sebum samples of 43 PD patients and 44 healthy controls (HCs) from Fourth Affiliated Hospital of Zhejiang University School of Medicine, China, were smelled by the AIO system. Univariate and multivariate methods were used to identify the significant volatile organic compound (VOC) features in the chromatograms. ML algorithms, including support vector machine, random forest (RF), k nearest neighbor (KNN), AdaBoost (AB), and Naive Bayes (NB), were used to distinguish PD patients from HC based on the VOC peaks in the chromatograms of sebum samples. Results: VOC peaks with average retention times of 5.7, 6.0, and 10.6 s, respectively, corresponding to octanal, hexyl acetate, and perillic aldehyde, were significantly different in PD and HC. The accuracy of the classification based on the significant features was 70.8%. Based on the odor profile, the classification had the highest accuracy and F1 of the five models with 0.855 from NB and 0.846 from AB, respectively, in the process of model establishing. The highest specificity and sensitivity of the five classifiers were 91.6% from NB and 91.7% from RF and KNN, respectively, in the evaluating set. Conclusions: The proposed AIO system can be used to diagnose PD through the odor profile of sebum. Using the AIO system is helpful for the screening and diagnosis of PD and is conducive to further tracking and frequent monitoring of the PD treatment process.
研究领域:
神经病学
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医学信息学
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机器学习
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临床化学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2021
·
IF 3.411
Ruixi Luo,Lifeng Zhao,Shuaishuai Li,Peng Chen,La Wang,Honghong Yu,Kun Cai,Qi Yu,Weiyi Tian
Excessive free fatty acid- (FFA-) induced endothelial lipotoxicity is involved in the pathogenesis of atherosclerosis. Endoplasmic reticulum (ER) stress is mechanistically related to endothelial lipotoxicity. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is the major oxidatively modified low-density lipoprotein (OxLDL) receptor in endothelial cells and is highly abundant in atherosclerotic lesions. Curcumin reduces the LOX-1 expression; however, the mechanism underlying this effect remains unknown. In the current study, we explored whether curcumin ameliorates palmitic acid- (PA-) induced endothelial lipotoxicity and LOX-1 upregulation by reducing ER stress in human umbilical vein endothelial cells (HUVECs). We built endothelial lipotoxicity in vitro and found that LOX-1 was upregulated after PA stimulation, during which ER stress played an important role. Next, we observed that curcumin substantially alleviated PA-induced lipotoxicity by restoring cell viability, increasing angiogenesis, and decreasing lipid deposition. Furthermore, LOX-1 upregulation in HUVECs was blocked by curcumin, possibly via ER stress suppression. Overall, our findings demonstrated that curcumin alleviates endothelial lipotoxicity and LOX-1 upregulation, and ER stress inhibition may play a critical role in this effect.
研究领域:
心血管研究
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Chi Zhang,Zhinan Fan,Hao Zhan,Hong Zhou,Rongliang Ma,Li-Juan Fan
Both latent sebaceous and blood fingerprints may provide valuable information for forensic investigation. To detect both types of fingerprints with no need to predistinguish them, a new adaptive developing strategy was proposed. A cationic conjugated polymer with poly[p-(phenylene ethylene)-alt-(thienylene ethynylene)] backbone (PPETE-NMe3+) was synthesized, which was dissolved in N,N-dimethylformamide (DMF) to form the developing solution. Fingerprints were developed by a simple dropping and incubating process without any pre-/post-treatments. Fluorescent photographs of the developed fingerprints on various substrates demonstrated that this developing strategy was effective for both types of fingerprints on nonporous substrates. Gray value analysis further confirmed the enhancement of the legibility of the fingerprint images. The preliminary mechanism exploration suggested that certain weak interactions, such as hydrophobic interaction and electrostatic interaction, may synergistically contribute to the interaction between the polymer and fingerprint components. The molecular design of the polymer combined with an appropriate solvent endowed the developing system the adaptiveness toward different types of fingerprints. This adaptive developing strategy made the fingerprint-developing process more efficient and may be further extended to more practical application scenes.
研究领域:
法医学
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聚合物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Ranran Kong,Sicheng Cao,Jincheng Zhang,Rongliang Ma,Li-Juan Fan
Finding a universal fingermark developing strategy effective for all types of fingermarks may improve the efficiency of forensic investigation. Here, we synthesized a fluorescent conjugated polymer (PTPEPE-IDA) with poly[p-(tetraphenylethene)-alt-(phenylene ethylene)] as the backbone and carrying pendant disodium iminodiacetate and monocarboxylate groups to interact with sweat, which is commonly present in fingermarks. PTPEPE-IDA was dissolved in ethanol to form the developing reagent, which was demonstrated to be effective in visualizing and enhancing sweat-containing sebaceous and blood fingermarks on various substrates with the same developing protocol. With the increase of the sweat content in fingermarks, the fluorescence intensity and clarity of patterns of the developed fingermarks increased. This strategy was effective for sweat-containing fingermarks aged for up to 100 days. The developed fingerprint has high stability, being recognizable after storage for 100 days or immersion in water (or ethanol). Mechanism investigation suggests that PTPEPE-IDA can bind some components in sweat and blood through synergistic effects of multiple interactions, and the aggregation-induced emission enhancement (AIEE) upon sweat was demonstrated by the macroscopic view and emission/absorption spectra, which is beneficial to detect the fingermark quickly and then enhance the legibility of the pattern. This universal strategy is expected to make fingermark development more efficient.
研究领域:
法医学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）