Chemical Engineering and Processing-Process Intensification
·
2021
·
IF 4.237
Weikun Li,Hongliang Wei,Yuhua Liu,Songmao Li,Gang Wang,Tao Guo,Huayun Han
Hydrogel microspheres (HMs) were prepared from hydroxyethyl starch and carboxymethyl chitosan via in situ reactive spray drying, using epichlorohydrin as the crosslinking agent. SEM characterization showed the HMs were spherical and collapsed structures. The swelling behavior and adsorption performance of the HMs were studied systematically. The experimental results demonstrated that HMs possessed pH and salt sensitiveness and the maximum adsorption capacities of HMs for methylene blue, Eosin Y, Cd 2+ , Cu 2+ , and Ni 2+ were 106.97 mg/g, 143.55 mg/g, 32.51 mg/g, 47.87 mg/g, and 27.18 mg/g, respectively. Further study indicated that the adsorption isotherms and kinetics were respectively fitted with the Langmuir isotherm model and the pseudo-second-order model. In view of the advantages of in situ reactive spray drying for the preparation of novel pH- and salt- responsive HMs, the strategy presented here has a great application potential in wastewater treatment.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Meitong Liu,Jing Lu,Qingbin Liu,Yuelin Chen,Guang Wang,Qian Zhang,Shuang Guan
Sodium metabisulphite (SMB) is the most used foods and drugs antioxidant among sulfites . So far, there were few studies about its harm, especially in mast cells. Our study was to investigate the effects of SMB on mitophagy and pyroptosis in mast cells. The results revealed that SMB dose-dependently promoted the expressions of NLRP3, GSDMD-N and other marker proteins of pyroptosis. Knockdown of GSDMD, NLRP3 inhibitor, mitophagy activator and mtROS inhibitor all reversed the changes in pyroptosis indicators caused by SMB. Considering the degranulation characteristics of mast cells and the sensitization of sulfite, we examined the effects of the above inhibitors on the degranulation of mast cells caused by SMB. The results showed that SMB-mediated mast cell degranulation was significantly inhibited by the above inhibitors. Meanwhile, we used immunofluorescence co-localization experiments and found that GSDMD pore-forming protein and histamine co-localized near the cell membrane. Overall, evidence suggested that SMB caused pyroptosis by inhibiting mitophagy, leading to mast cell degranulation. These findings are of great significance to the sensitization mechanism of SMB and provide a new insight into SMB toxicology and mast cell degranulation.
研究领域:
毒理学
·
免疫学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TREE PHYSIOLOGY
·
2024
·
IF 3.5
Liu Jian,Li Jing,Deng Chen,Liu Zhe,Yin Kexin,Zhang Ying,Zhao Ziyan,Zhao Rui,Zhao Nan,Zhou Xiaoyang,Chen Shaoliang
Nitrogen (N) plays an important role in mitigating salt stress in tree species. We investigate the genotypic differences in the uptake of ammonium (NH4+) and nitrate (NO3−) and the importance for salt tolerance in two contrasting poplars, salt-tolerant Populus euphratica and salt-sensitive P. popularis. Total N content, growth, and photosynthesis were significantly reduced in P. popularis after 7 days exposure to NaCl (100 mM) supplied with 1 mM NH4+ and 1 mM NO3−, while the salt effects were not pronounced in P. euphratica. The 15NH4+ trace and root flux profiles showed that salt-stressed poplars retained ammonium uptake, which was related to the up-regulation of ammonium transporters (AMTs) in roots, as two of the four AMTs tested significantly increased in salt-stressed P. euphratica (i.e., AMT1.2, 2.1) and P. popularis (i.e., AMT1.1, 1.6). It should be noted that P. euphratica differs from salt-sensitive poplar in the maintenance of NO3− under salinity. 15NO3− tracing and root flux profiles showed that P. euphratica maintained nitrate uptake and transport, while the capacity to uptake NO3− was limited in salt-sensitive P. popularis. Salt increased the transcription of nitrate transporters (NRTs), NRT1.1, 1.2, 2.4, 3.1, in P. euphratica, while P. popularis showed a decrease in the transcripts of NRT1.1, 2.4, 3.1 after 7 days of salt stress. Furthermore, salt-stimulated transcription of plasmalemma H+-ATPases (HAs), HA2, HA4 and HA11 contributed to H+-pump activation and NO3− uptake in P. euphratica. However, salt stimulation of HAs was less pronounced in P. popularis, where a decrease in HA2 transcripts was observed in the stressed roots. We conclude that the salinity-decreased transcripts of NRTs and HAs reduced the ability to uptake NO3− in P. popularis, resulting in limited nitrogen supply. In comparison, P. euphratica maintains NH4+ and NO3− supply, mitigating the negative effects of salt stress.
研究领域:
植物学
·
植物生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHIMIE
·
2023
·
IF 3.9
Ya-nan Ou-Yang,Fen-fen Deng,Yun-jia Wang,Meng Chen,Peng-fei Yang,Zhe Yang,Zhongmin Tian
A high-salt diet is known to increase serum cholesterol levels; however, the underlying mechanism of salt - induced dyslipidemia in patients with salt-sensitivity remains poorly understood. We aimed to investigate whether high-salt diet (HSD) can induce dyslipidemia and elucidate the underlying mechanism of salt-induced dyslipidemia in Dahl salt - sensitive (SS) rats. Metabolomic and biochemical analyses revealed that the consumption of an HSD (8 % NaCl) significantly increased the serum levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL - C) in SS rats. The enzyme-linked immunosorbent assay demonstrated an increase in circulating proprotein convertase subtilisin/kexin type 9 (PCSK9) levels, accompanied by a decrease in hepatic low-density lipoprotein receptor (LDLR) levels due to HSD consumption. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis revealed that HSD consumption activated sterol regulatory element-binding protein-2 (SREBP2) expression in the liver and kidney, resulting in upregulation of PCSK9 at the transcriptional level in the liver and at the translational level in the kidney, ultimately increasing circulating PCSK9 levels. The combined effects of HSD on the liver and kidney contributed to the development of hypercholesterolemia. Furthermore, an in vitro assay confirmed that high-salt exposure led to an increase in the protein expression of SREBP2 and PCSK9 secretion, thereby reducing low-density lipoprotein (LDL) uptake. This study, for the first time, shows that an HSD induces dyslipidemia through activation of the SREBP2/PCSK9 pathway, providing new insights into the prevention and treatment of dyslipidemia in patients with salt sensitivity.
研究领域:
营养学
·
心血管疾病
·
分子生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2023
·
IF 8.9
Peng Yan,Xuewei Zheng,Shuang Liu,Yanhua Dong,Tao Fu,Zhongmin Tian,Yayan Wu
Rapid and efficient detection and identification of proteins hold great promise in medical diagnostics, treatment of different diseases, and proteomics. Here, we present a simple colorimetric sensor array for the differentiation of proteins in various osmolyte solutions. Osmolytes have different influences on the conformation of proteins, which have differential binding to silver nanoparticles, resulting in color changes. The sensor array shows unique color change patterns for each of the 19 proteins, allowing unambiguous identification. Very interestingly, the differentiation of 19 proteins is related to their molecular weight. Moreover, the sensor array can be used to identify protein mixtures, thermal denaturized proteins, and unknown protein samples. Finally, the sensor array can also analyze the plasma or liver samples of the four groups of salt-sensitive rats fed with different diets, indicating that it has the potential for the classification of metabolic profiles.
研究领域:
医学诊断
·
蛋白质组学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）