Aquaculture Reports
·
2023
·
IF 3.7
Xinyu Song,Wengang Lü,Salifu Ibrahim,Yuewen Deng,Qi Li,Chenyang Yue
Crassostrea hongkongensis (Hong Kong oyster), a commercially valuable bivalve thriving in the estuaries along the northern coast of the South China Sea, is threatened by the increase of salinity during the dry season. It is essential to improve its hyper-salinity tolerance, which requires a clear understanding of the mechanism to regulate its osmotic balance. For this purpose, 25 free amino acids (FAA), the most critical intracellular osmolyte in bivalves, were quantified in gill, adductor muscle, and hemolymph of C. hongkongensis during abrupt salinity changes (18–6, 12, 24 and 30‰) by high-performance liquid chromatography-tandem mass spectrometry. Taurine, alanine and glycine were dominant FAA, contributing to total FAA contents that varied with salinities, no matter in gill, muscle or hemolymph. Under hypo-osmotic stress, total FAA contents decreased in gill and adductor muscle but increased in hemolymph, probably resulting from the extrusion of some FAA from tissues to prevent swelling of cells. And taurine contributed most significantly to the hypo-salinity response, which was mainly reflected by the remarkable increase in hemolymph at 8 h after the exposure to 6‰. By contrast, total FAA contents increased in gill, muscle, and hemolymph when transferred to hyper-salinity, suggesting the assumption that it increases the osmolality and subsequently prevents the cell shrinking. Alanine and glycine contributed most significantly to hyper-salinity response. Of the two, the former was more sensitive to hyper-osmotic stress for its rapid increase in gill within 8 h after the exposure to 24 and 30‰. An inverse pattern with most FAA was presented by ornithine, the intermediate of the urea cycle, which was probably associated with the FAA metabolism. This study identified the major FAA as intracellular osmolytes in C. hongkongensis and would benefit the further understanding of the regulatory mechanisms of oyster salinity acclimation.
研究领域:
海洋生物学
·
分子生物学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Chunli Yu,Yao Hu,Yuqin Zhang,Wei Luo,Jing Zhang,Peilun Xu,Jun Qian,Jun Li,Jianfeng Yu,Jin Liu,Wenguang Zhou,Shengxi Shao
In industrial-scale cultivation of microalgae, salinity stress often stimulates high-value metabolites production but decreases biomass yield. In this research, we present an extraordinary response of Arthrospira platensis to salinity stress. Specifically, we observed a significant increase in both biomass production (2.58 g L −1 ) and phycocyanin (PC) content (22.31%), which were enhanced by 1.26-fold and 2.62-fold, respectively, compared to the control, upon exposure to exogenous glycine betaine (GB). The biochemical analysis reveals a significant enhancement in carbonic anhydrase activity and chlorophyll a level, concurrent with reductions in carbohydrate content and reactive oxygen species (ROS) levels. Further, transcriptomic profiling indicates a downregulation of genes associated with the tricarboxylic acid (TCA) cycle and an upregulation of genes linked to nitrogen assimilation, hinting at a rebalanced carbon/nitrogen metabolism favoring PC accumulation. This work thus presents a promising strategy for simultaneous enhancement of biomass production and PC content in A. platensis and expands our understanding of PC biosynthesis and salinity stress responses in A. platensis .
研究领域:
生物技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Sibo Wang,Jingsong Guo,Yanyan Peng,Jing Han,Zhaowen Jiang,Xiaoxue Hu,Shaoqing Gao,Xiaoxia Wu,Wei Ji
The 26S proteasome is a crucial protease complex responsible for degrading specific proteins to maintain cellular function during salt stress. Previous studies have shown that GmRPN11d, a subunit of the regulatory particle in soybean, is upregulated in response to short-term salt stress. This research discovered that GmRPN11d is localized in the nucleus and cytoplasm, with its expression increasing under high salinity and other stress conditions. Overexpressing GmRPN11d in Arabidopsis and soybean hairy roots significantly improves salt stress tolerance. Examination of physiological indices and expression patterns of salt-responsive marker genes reveals that overexpression of GmRPN11d enhances the ability to scavenge reactive oxygen species, regulates ion balance, exhibits hypersensitivity to ABA, and activates the ABA signaling pathway under salt stress. Additionally, GmRPN11d was demonstrated to interact with the SUMO E3 ligase GmMMS21 in both in vivo and in vitro experiments. This interaction serves to facilitate the SUMOylation of GmRPN11d, ultimately contributing to its stability when faced with salt stress. Taken together, these findings highlight the role of GmRPN11d in promoting plant salt tolerance through SUMOylation, mediated by GmMMS21. This study provides valuable insights into modifying the 26S proteasome subunit in soybean, offering a potential target gene for developing genetically modified salt-resistant crops.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Zhanyu Shen,Fanghua Mo,Lei Wang,Yu Su,Huijing Han,Kaiyue Zheng,Chunling Wang
The ARTP (atmospheric and room temperature plasma) mutation breeding approach was employed to enhance the salt tolerance and metabolic capability of Torulopsis versatilis . Targeted screening for salt tolerance identified the positive mutant strain T20-3, which exhibited improved performance in media 18 % and 20 % salinity. The selected strain's genetic stability, metabolic performance, and fermentation characteristics were further evaluated through successive passaging and mash fermentation experiments. The results demonstrated that T20-3 yeast could maintain stable propagation under high salt conditions and exhibited robust flavor production through enhanced glycolysis, amino acid metabolism, and fatty acid metabolism pathways. During the fermentation of T20-3, the content of alcohols and esters was elevated by 95.28 % and 201.83 %, with a significant increase in isoamyl acetate and phenylethanol. The combination of headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analysis and sensory evaluation demonstrated that increased levels of key flavor compounds, including phenylethanol, acetic acid, isoamyl acetate, 3-hydroxy-2-butanone, and 2,3,5-trimethylpyrazine, contributed to a more pronounced floral, fruity, and sauce-like flavor profile in T20-3 soy sauce. In conclusion, the findings indicated that T20-3 enhanced the flavor quality of fermented soy sauce and holds considerable potential for improving fruity, floral, umami, and aftertaste profiles.
研究领域:
食品科学
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微生物学
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代谢组学
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY
·
2024
·Vol. 197
·期 1
·
IF 6.5
Song Ru-Feng,Liao Cai-Yi,Wang Lin-Feng,Lu Kai-Kai,Zhang Chi,Wu Run-Xin,Wu Ji-Xiao,Ma Yu-Qing,Kuang Lei,Guo Ning,Yuan Hong-Mei,Liu Wen-Cheng
The plasma membrane (PM)-localized Na+/H+ antiporter Salt Overly Sensitive1 (SOS1) is essential for plant salt tolerance through facilitating Na+ efflux; however, how SOS1 localization and protein accumulation is regulated in plants remains elusive. Here, we report that Sorting Nexin 1 (SNX1) is required for plant salt-stress tolerance through affecting endosomal trafficking of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress. SNX1 co-localized and interacted with SOS1 in endosomes, promoting its PM localization and protein stability in plants under saline conditions. SOS1 overexpression promoted salt tolerance in the wild-type, whereas such effect was greatly compromised in the snx1-2 mutant. Pharmaceutical results showed that SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant. Furthermore, salt-induced SOS1 phosphorylation enhanced its interaction and co-localization with SNX1, which is required for SOS1 PM localization in plants. Our study elucidates that SNX1 facilitates SOS1 PM localization and protein accumulation through endosomal trafficking, thereby enhancing salt tolerance in plants.
研究领域:
植物学
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分子生物学
✓ 已验证
DOI
高影响因子
METABOLIC ENGINEERING
·
2013
·
IF 6.859
Wei Du,Feiyan Liang,Yangkai Duan,Xiaoming Tan,Xuefeng Lu
Because cyanobacteria are photosynthetic, fast-growing microorganisms that can accumulate sucrose under salt stress, they have a potential application as a sugar source for the biomass-derived production of renewable fuels and chemicals. In the present study, the production of sucrose by the cyanobacteria Synechocystis sp. PCC6803, Synechococcus elongatus PCC7942, and Anabaena sp. PCC7120 was examined. The three species displayed different growth curves and intracellular sucrose accumulation rates in response to NaCl. Synechocystis sp. PCC6803 was used to examine the impact of modifying the metabolic pathway on the levels of sucrose production. The co-overexpression of sps ( slr0045 ) , spp ( slr0953 ), and ugp ( slr0207 ) lead to a 2-fold increase in intracellular sucrose accumulation, whereas knockout of ggpS ( sll1566 ) resulted in a 1.5-fold increase in the production of this sugar. When combined, these genetic modifications resulted in a fourfold increase in intracellular sucrose accumulation. To explore methods for optimizing the transport of the intracellular sucrose to the growth medium, the acid-wash technique and the CscB (sucrose permease)-dependent export method were evaluated using Synechocystis sp. PCC6803. Whereas the acid-wash technique proved to be effective, the CscB-dependent export method was not effective. Taken together, these results suggest that using genetic engineering, photosynthetic cyanobacteria can be optimized for efficient sucrose production.
研究领域:
微生物学
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遗传学
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生物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANNALS OF BOTANY
·
2023
·Vol. 131
·期 4
·723-736
·
IF 4.2
Li Hu-Jun,Bai Wan-Peng,Liu Lin-Bo,Liu Hai-Shuang,Wei Li,Garant Timothy M,Kalinger Rebecca S,Deng Yu-Xuan,Wang Gai-Ni,Bao Ai-Ke,Ma Qing,Rowland Owen,Wang Suo-Min
Background and AimsDesert plants possess excellent water-conservation capacities to survive in extreme environments. Cuticular wax plays a pivotal role in reducing water loss through plant aerial surfaces. However, the role of cuticular wax in water retention by desert plants is poorly understood.MethodsWe investigated leaf epidermal morphology and wax composition of five desert shrubs from north-west China and characterized the wax morphology and composition for the typical xerophyte Zygophyllum xanthoxylum under salt, drought and heat treatments. Moreover, we examined leaf water loss and chlorophyll leaching of Z. xanthoxylum and analysed their relationships with wax composition under the above treatments.Key ResultsThe leaf epidermis of Z. xanthoxylum was densely covered by cuticular wax, whereas the other four desert shrubs had trichomes or cuticular folds in addition to cuticular wax. The total amount of cuticular wax on leaves of Z. xanthoxylum and Ammopiptanthus mongolicus was significantly higher than that of the other three shrubs. Strikingly, C31 alkane, the most abundant component, composed >71 % of total alkanes in Z. xanthoxylum, which was higher than for the other four shrubs studied here. Salt, drought and heat treatments resulted in significant increases in the amount of cuticular wax. Of these treatments, the combined drought plus 45 °C treatment led to the largest increase (107 %) in the total amount of cuticular wax, attributable primarily to an increase of 122 % in C31 alkane. Moreover, the proportion of C31 alkane within total alkanes remained >75 % in all the above treatments. Notably, the water loss and chlorophyll leaching were reduced, which was negatively correlated with C31 alkane content.ConclusionZygophyllum xanthoxylum could serve as a model desert plant for study of the function of cuticular wax in water retention because of its relatively uncomplicated leaf surface and because it accumulates C31 alkane massively to reduce cuticular permeability and resist abiotic stressors.
研究领域:
植物学
·
生态学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2019
·
IF 4.106
Hussain Sajid,Bai Zhigang,Huang Jie,Cao Xiaochuang,Zhu Lianfeng,Zhu Chunquan,Khaskheli Maqsood Ahmed,Zhong Chu,Jin Qianyu,Zhang Junhua
Salt stress in soil is a critical constraint that affects the production of rice. Salt stress hinders plant growth through osmotic stress, ionic stress, and a hormonal imbalance (especially ethylene), therefore, thoughtful efforts are needed to devise salt tolerance management strategies. 1-Methylcyclopropene (1-MCP) is an ethylene action inhibitor, which could significantly reduce ethylene production in crops and fruits. However, 1-MCPs response to the physiological, biochemical and antioxidant features of rice under salt stress, are not clear. The present study analyzed whether 1-MCP could modulate salt tolerance for different rice cultivars. Pot culture experiments were conducted in a greenhouse in 2016–2017. Two rice cultivars, Nipponbare (NPBA) and Liangyoupeijiu (LYP9) were used in this trial. The salt stress included four salt levels, 0 g NaCl/kg dry soil (control, CK), 1.5 g NaCl/ kg dry soil (Low Salt stress, LS), 4.5 g NaCl/kg dry soil (Medium Salt stress, MS), and 7.5 g NaCl/kg dry soil (Heavy Salt stress, HS). Two 1-MCP levels, 0 g (CT) and 0.04 g/pot (1-MCP) were applied at the rice booting stage in 2016 and 2017. The results showed that applying 1-MCP significantly reduced ethylene production in rice spikelets from LYP9 and NPBA by 40.2 and 23.9% (CK), 44.3 and 28.6% (LS), 28 and 25.9% (MS), respectively. Rice seedlings for NPBA died under the HS level, while application of 1-MCP reduced the ethylene production in spikelets for LYP9 by 27.4% compared with those that received no 1-MCP treatment. Applying 1-MCP improved the photosynthesis rate and SPAD value in rice leaves for both cultivars. 1-MCP enhanced the superoxide dismutase production, protein synthesis, chlorophyll contents (chl a, b, carotenoids), and decreased malondialdehyde, H2O2, and proline accumulation in rice leaves. Application of 1-MCP also modulated the aboveground biomass, and grain yield for LYP9 and NPBA by 19.4 and 15.1% (CK), 30.3 and 24% (LS), 26.4 and 55.4% (MS), respectively, and 34.5% (HS) for LYP9 compared with those that received no 1-MCP treatment. However, LYP9 displayed a better tolerance than NPBA. The results revealed that 1-MCP could be employed to modulate physiology, biochemical, and antioxidant activities in rice plants, at different levels of salt stress, as a salt stress remedy.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Renzhang Lin,Hao Li,Hao Wu,Hao Ren,Xiangyu Kong,Zhenmei Lu
The intrinsic issue associated with the application of microbes for practical pollution remediation involves maintaining the expected activity of engaged strains or consortiums as effectively as that noted under laboratory conditions. Faced with various stress factors, degraders with dormancy ability are more likely to survive and exhibit degradation activity. In this study, a hydrocarbonoclastic and halotolerant strain, Gordonia polyisoprenivorans ZM27, was isolated via stimulation with resuscitation-promoting factor (Rpf). Long-term exposure to dual stresses of 10% NaCl and starvation induced ZM27 to enter a viable but nonculturable (VBNC)-like state, and ZM27 cells could be resuscitated upon Rpf stimulation. Notable changes in both morphological and physiological characteristics between VBNC-like ZM27 cells and resuscitated cells confirmed the response to Rpf and their robust resistance against harsh environments. Whole-genome sequencing and analysis indicated ZM27 could be a generalist degrader with dormancy ability. Subsequently, VBNC-like ZM27 was applied in a soil microcosm experiment to investigate the practical application potential under harsh conditions. VBNC-like ZM27 combined with Rpf stimulation exhibited the most effective biodegradation performance, and the initial n -hexadecane content (1000 mg kg −1 ) decreased by 63.29% after 14-day incubation. Based on 16S rRNA amplicon sequencing and analysis, Gordonia exhibited a positive response to Rpf stimulation. The relative abundance of genus Gordonia was negatively correlated with that of Alcanivorax , a genus of obligate hydrocarbon degrader with the greatest abundance during soil incubation. Based on the degradation profile and community analysis, generalist Gordonia may be more efficient in hydrocarbon degradation than specialist Alcanivorax under harsh conditions. The characteristics of ZM27, including its sustainable culturability under long-term stress, response to Rpf and robust performance in soil microcosms, are valuable for the remediation of petroleum pollution under stressful conditions. Our work validated the importance of dormancy and highlighted the underestimated role of low-activity degraders in petroleum remediation.
研究领域:
微生物学
·
环境科学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Protocols
·
2024
·
IF 13.1
Ma Xiaoxie,Zeng Xiaoyan,Huang Yurou,Liu Sheng Hua,Yin Jun,Yang Guang-Fu
Salt stress is an adverse environmental condition that harms plant growth and development. The development of salt stress probes is critical for tracking the growth dynamics of plants, molecular breeding or screening of growth regulators. The sodium chloride (NaCl)-responsive fluorescent probe Aza-CyBz is designed based on the tenet that NaCl induces formation of ordered aggregates, and the sensitive fluorescence response can enable the visualization of plant salt stress in root tip tissues and live plants. Herein, we describe a detailed three-step route for synthesis of Aza-CyBz and applications to monitoring salt stress in Arabidopsis thaliana . The procedures for operating fluorescence imaging under various stresses are also listed to eliminate interference from the oxidative mechanism of salt stress. Compared with conventional invasive approaches such as inductively coupled plasma emission spectrometry and flame photometer, our protocol can real-time monitor salt stress experienced by plants, which demands simple pretreatment procedure and staining technique. Due to near infrared fluorescence, this method provides direct visual observation of salt stress at both tissue and live plant levels, which is superior to conventional noninvasive approaches. The preparation of probe Aza-CyBz takes ~2 d, and the imaging experiments for assessing salt stress experienced by plants, including the preparation of stressed plant samples takes ~9–11 d for root tip tissues and ~23 d for live plants. Notably, acquisition and analysis visual images of salt stress in plants can be completed within 2 h and they require only a basic knowledge of spectroscopy and chemistry.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 11
·1576
·
IF 4.1
Zhangting Xu,Feixiong Zheng,Xiaoji Deng,Yiming Sun,Zhenming Yu,Xiaoxia Shen
Dendrobium officinale,which was rich in bioactive compounds such as polysaccharides, alkaloids, amino acids, and flavonoids, had significant medicinal value and ability to resist stresses. Studies had demonstrated thatGATAgenes were one of the crucial regulators in controlling plant development and growth and stress response. Genome-wide identification and characterization of the 18DoGATAgenes were displayed. According to phylogenetic relationships, theDoGATAfamily genes were divided into 4 groups and the conserved motifs ofDoGATA1–DoGATA18within the same group were similar. AllDoGATAgenes were localized in the nucleus and randomly mapped on 10 chromosomes. The GATA genes inD. officinaleexperienced one pair of tandem duplication and 4 pairs of segment duplications to expand the family genes. Additionally, we found that the 2000 bp upstream promoter region of theDoGATAgenes harbored 23 types ofcis-acting elements that were categorized into plant growth and development, phytohormone responsiveness, and stress responsiveness.DoGATA1–DoGATA18were diversely expressed across different tissues (root, leaf, stem, flower), exposed to salt stress, and following MeJA treatment. Co-expression analysis betweenDoGATAand enzyme-encoding genes involved in the biosynthesis of flavone showed thatDoCHI(LOC110104562) andDoGTMT(LOC110098370) may be potential downstream targets ofDoGATA16to regulate flavonoid biosynthesis to adapt to salt stress. Furthermore, we confirmed thatDoGATA16may act as a key member to resist stress. The collective findings of this study shed light on the function ofGATAgenes and molecular breeding ofD. officinale.
研究领域:
植物学
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分子生物学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Du Jiang,Xu Huazhen,Zhang Da-xia,Feng Shouqian
Soil salinization hinders sustainable development of global agriculture. Dopamine (DA) delivery is promising for mitigating the detrimental effects of salt on plants. However, self-polymerization limits delivery and effectiveness. Here we chelated DA with ethylenediamine tetraacetic acid and zinc to reduce self-polymerization. To reduce soil adsorption, a sodium lignosulfonate and octadecyl dimethyl benzyl ammonium chloride nanocarrier is made for delivery to the plant. Compared with DA monomer, the soil adsorption rate of the DA in the nanocarrier is 46.02% lower. Salt stress experiments reveal, compared with NaCl and DA groups, the nanocarrier group exhibits significant increases in growth indicators for tomato plants. The beneficial effect is attributed to the increases in proline content, antioxidant capacity, and K + /Na + ratios in the plants. Similar results are also observed with woody pear seedlings. These findings provide insights into alleviating crop salt stress.
研究领域:
植物学
·
农业科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）