Materials Today Bio
·
2026
·
IF 10.2
Chen Chen,Na Zhang,Fructueux Modeste Amona,Xiaolei Han,Qi Tang,Lanxin Geng,Guangfu Liao,Jie Zhang,Tushuai Li
Chronic wounds infected with multidrug-resistant bacteria pose a significant challenge to global health, as traditional monotherapies often fail to eradicate pathogens and restore the damaged wound microenvironment. Engineering innovative nanocomposites that integrate synergistic therapeutics and controlled release is a pivotal strategy for advanced wound management. Here, we developed a novel multi-drug metal-organic framework (MOF) nanocomposite, BBR + FA@UiO, by covalently grafting ferulic acid (FA) onto UiO-66-NH 2 and physically loading berberine (BBR). Drawing from "Jun–Chen–Zuo–[ Shi ]" hierarchy of Traditional Chinese Medicine (TCM), this design used the MOF as a robust porous carrier “[ Shi ]” for the antibacterial drug "Jun" (BBR) and antioxidant drug "Chen/Zuo" (FA). BBR + FA@UiO exhibits potent, synergistic antibacterial and antibiofilm activity against critical clinical pathogens like MRSA and Pseudomonas aeruginosa, through a pH/ROS-responsive release mechanism in the infected site. Functionally, BBR + FA@UiO activates the Nrf2/Keap1 pathway in macrophages, thereby mitigating oxidative stress and enhancing mitochondrial function. Consequently, proliferation and migration of Human Umbilical Vein Endothelial Cells (HUVECs) are restored through promoting angiogenesis. In vivo, BBR + FA@UiO significantly accelerates healing by eradicating bacteria, enhancing collagen deposition, and improving vascularization, driven by the coordinated activation of the Nrf2/HO-1 antioxidant axis and pro-regenerative Wnt/β-catenin and TGF-β signaling pathways. Demonstrating excellent biocompatibility and no systemic toxicity, this work establishes BBR + FA@UiO as a new therapeutic approach that merges TCM with advanced MOFs for comprehensive wound care.
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高影响因子
PHYTOTHERAPY RESEARCH
·
2026
·
IF 6.3
Zijun Cao,Jinfeng Duan,Shan Meng,Zijun Zhou,Yuting Huang,Xin Chen,Jikai Zhao,Tao Huang,Guoxin Zhang,Tong Su,Liming Yu,Zongtao Yin,Huishan Wang
Heart failure with preserved ejection fraction (HFpEF) and atrial fibrillation (AF) frequently coexist due to shared risk factors, yet optimal therapeutic interventions remain elusive. Berberine (BBR), a widely used isoquinoline alkaloid, has demonstrated potential anti-arrhythmic properties. However, its specific effects and regulatory mechanisms in HFpEF-related AF are not fully understood. The present study was designed to clarify the pathological characteristics of HFpEF-associated AF and further evaluate the therapeutic effects of BBR. In this study, we developed a high-fat diet plus Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced murine HFpEF model to investigate the impact of berberine on the pathogenesis of HFpEF-associated AF. Bioinformatics and in vivo analyses revealed a progressive increase in atrial endoplasmic reticulum (ER) stress and lipid metabolic dysregulation. The HFpEF model exhibited significant atrial structural and electrical remodeling, which was dose-dependently reversed by berberine treatment. Further investigation showed that berberine effectively preserved atrial lipid metabolism, reduced ER stress and atrial inflammation, and restored atrial AMP-activated protein kinase (AMPK) activity. These protective effects were abolished by Compound C treatment, yet replicated by metformin or 4-phenylbutyric acid administration. Notably, the beneficial effects of berberine were validated in an isoprenaline/palmitic acid (ISO/PA)-treated HL-1 cell model. Our study highlights the therapeutic potential of berberine in mitigating HFpEF-associated atrial ER stress, lipid accumulation, and inflammatory responses. By targeting and preserving atrial AMPK signaling, berberine offers a promising therapeutic approach to combat HFpEF-associated AF.
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MACROMOLECULAR BIOSCIENCE
·
2026
·Vol. 26
·期 8
·e70242
·
IF 4.2
Linhan Hu,Zheng Yang,Xinwei Tao,Bowen Ren,Tianle Zhou,Jianliang Li,Huaping Tan,Xiaohong Hu
Hydrogel-based dressings are widely used in wound healing. Herein, we report a polyacrylic acid (PAA)/polyvinyl alcohol (PVA) composite hydrogel fabricated via self-catalyzed free radical polymerization, with borax serving as the reinforcing phase. Meanwhile, metal-ligand coordination bonding between tannic acid (TA) and Fe 3 + further elevates the crosslinking density of the hydrogel network. Magnetic chitosan microspheres (MCMs) were synthesized by emulsion cross-linking and loaded with two antibacterial agents, namely tetracycline hydrochloride (TH) and berberine hydrochloride (Bbh). The incorporation of MCMs into the hydrogel matrix resulted in the development of a multifunctional composite hydrogel suitable for wound dressing applications. Results demonstrated that the composite hydrogel containing a specific concentration of 4‰ (w/v) borax and 20 mg/mL MCMs exhibited superior performance, including enhanced mechanical strength, improved responsiveness, sustained drug release, and potent antibacterial efficacy. The core novelty of this work lies in the synergistic integration of borax-based mechanical reinforcement, MCM-mediated dual drug loading and sustained release, and the self-catalyzed polymerization system. This innovative structural and functional collaboration effectively optimizes the mechanical stability of the hydrogel dressing and achieves synergistic antibacterial and intelligent responsive therapeutic performances, providing a reliable and high-efficiency candidate for advanced wound care and next-generation wound dressing applications.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-21
·
IF 5.7
Wei Ji-chang,Lin Xiao-yu,Zhao Yi-hang,Tan Xin-ru,Wang Zhi-xia,Li Yuan-yuan,Huang Xue-mei,Wang Peng-long
Background Scutellariae Radix (SR) and Coptidis Rhizoma (CR) are classic drug pairs used in clinical practice for clearing heat and drying dampness, purging fire for removing toxin. By further studying the mechanism of compatibility of SR and CR from the perspective of thermodynamically driven supramolecular phase transition, we could reveal the interaction between its pharmacodynamic components, and provide scientific basis for improving TCM efficacy. Methods The SR-CR and its main components baicalin-berberine (BA-BBR) were taken as the research objects. The morphology of the mechanically mixed samples was characterized by malvern particle size analyzer and scanning electron microscope. UHPLC-Q-Orbitrap HRMS technology was employed to analyze the material basis of each mechanically mixed sample. ITC was used to investigate the effect of temperature on the binding ability between SR and CR. The structural differences of supramolecules in different morphology were explored by molecular dynamics simulation. Finally, in vitro antibacterial models ( E. faecium and B. subtilis , S. aureus ) were used to evaluate the antibacterial activities of the mechanically mixed samples and non-targeted metabolomics was used to explore the differences in antibacterial mechanisms. Results The mechanical mixtures formed nanofibers (NFs), while heating induced a transition to nanospheres (NPs). Molecular dynamics simulations revealed that enhanced hydrogen bonding and tighter molecular packing under thermal conditions drove this morphological shift. In vitro antibacterial assays and non-targeted metabolomics showed NPs exhibited superior inhibition against Staphylococcus aureus by disrupting amino acid biosynthesis and metabolism, whereas NFs suppressed Bacillus subtilis via physical entanglement and interfered with energy metabolism. Conclusion Driven by thermal energy, the existence form of supramolecules changed from NFs to NPs and the morphology of the formed supramolecules was maintained during their interaction with bacteria, further affected the biological activity. Graphical
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Xiaoyan Mu,Yongqi Liu,Jiajia Zhang,Yawen Yin,Anchi Diao,Yixin Jiao,Shiyu Chen,Di Xu,Gang Li,Zhaozhu Zheng,Xiaoqin Wang
Chronic and contaminated wounds are characterized by concurrent bacterial infection and excessive reactive oxygen species (ROS), necessitating biomaterials capable of coordinated antimicrobial and antioxidant regulation. In this study, we developed an all-silk fibroin–based dual-drug delivery system through macromolecular assembly of distinct silk micro/nanostructures. Berberine (BBR) was electrostatically immobilized onto negatively charged silk micro/nanofibers to enable rapid antibacterial release, while curcumin (CUR) was encapsulated within silk fibroin nanoparticles formed via PEG-induced self-assembly to achieve sustained ROS scavenging. The two functional modules were integrated into a porous silk fibroin matrix through lyophilization, yielding a mechanically compliant and structurally interconnected delivery platform. The system exhibited structure-governed staged release behavior, in which electrostatic adsorption enabled rapid BBR diffusion while β-sheet–stabilized hydrophobic confinement regulated sustained CUR transport. In vitro studies demonstrated strong antibacterial activity, effective radical-scavenging capacity, and favorable cytocompatibility. In a full-thickness murine wound model, the optimized formulation accelerated early-stage wound healing and enhanced histological indicators of repair, including improved epithelial continuity and collagen deposition. These results demonstrate how hierarchical silk fibroin organization can couple molecular interaction strength with programmed drug transport, offering a versatile strategy for multifunctional wound-healing biomaterials.
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Jingliang Wu,Cuiping Qi,Hao Wang,Qing Wang,Jingui Sun,Jinping Dong,Guohua Yu,Zhiqin Gao,Bo Zhang,Guixiang Tian
Cancer-associated fibroblasts (CAFs) are a major component of the tumor microenvironment (TME). In hepatocellular carcinoma (HCC), quiescent hepatic stellate cells (HSCs) could be activated to become CAFs, which play a critical role in tumor progression and drug resistance. Therefore, recent efforts have been focused on combining anti-HSC and pro-apoptotic activities to improve anti-tumor efficacy of drugs. In this study, glycyrrhetinic acid and hyaluronic acid–modified liposomes (GA-HA-Lip) were prepared for co-delivery of curcumin (CUR) and berberine (BBR) for the treatment of HCC. Furthermore, we established the LX-2+BEL-7402 co-cultured cell model and implanted the m-HSCs+H22 cells into a mouse to evaluate the anti-tumor effect of CUR&BBR/GA-HA-Lip both in vitro and in vivo . The results showed that CUR&BBR/GA-HA-Lip could accumulate in tumor tissues and be taken up by HSCs and BEL-7402 cells simultaneously. Compared with free CUR, the combination therapy based on GA-HA-Lip exhibits stronger pro-apoptotic and anti-proliferation effect both in vitro and in vivo . The anti-tumor mechanistic study revealed that CUR&BBR/GA-HA-Lip could inhibit the activation of HSCs and restrain drug resistance of tumor cells. In summary, CUR&BBR/GA-HA-Lip could be a promising nano-sized formulation for anti-tumor therapy.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-15
·
IF 9.429
Huang Xuemei,Liu Xiaojing,Lin Xiaoyu,Yuan Zhihua,Zhang Yaozhi,Wang Zhijia,Pi Wenmin,Zhao Haoqiang,Lei Haimin,Wang Penglong
Through the self-assembled strategy to improve the clinical efficacy of the existing drugs is the focus of current research. Herbal formula granule is a kind of modern dosage form of traditional Chinese medicine (TCM) which has sprung up in recent decades. However, whether it is equivalent to the TCM decoction that has been used for thousands of years has always been a controversial issue. In this paper, taking the herb pair of Coptidis Rhizoma—Scutellariae Radix and its main component berberine—baicalin as examples, the differences and mechanisms of self-assemblies originated from the co-decoction and physical mixture were studied, respectively. Moreover, the relationship between the morphology and antibacterial effects of self-assemblies was illuminated via multi-technology. Our study revealed that the physical mixture’s morphology of both the herb pair and the phytochemicals was nanofibers (NFs), while their co-decoction’s morphology was nanospheres (NPs). We also found that the antibacterial activity was enhanced with the change of self-assemblies’ morphology after the driving by thermal energy. This might be attributed to that NPs could influence amino acid biosynthesis and metabolism in bacteria. Current study provides a basis that co-decoction maybe beneficial to enhance activity and reasonable use of herbal formula granule in clinic.
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JOURNAL OF CONTROLLED RELEASE
·
2022
·
IF 11.467
Longfa Kou,Huirong Huang,Yingying Tang,Meng Sun,Yingtao Li,Jianing Wu,Shimin Zheng,Xinyu Zhao,Daosen Chen,Zucheng Luo,Xiaolei Zhang,Qing Yao,Ruijie Chen
Osteoarthritis (OA) is a chronic disease caused by joint inflammation . Its occurrence and development depend on a continuous inflammation environment. The activated M1 macrophages play a critical role in the inflammatory response of OA. Regulating the pro-inflammatory M1 to anti-inflammatory M2 macrophages in the OA articular cavity could be a rational strategy for OA treatment. It has been acknowledged that activated macrophages could proactively capture opsonized nanoparticles in the bloodstream and then accumulate into the reticuloendothelial system (RES) organs. Based on this fact, a trapping strategy is proposed, which transforms a normal nanoparticle into an opsonized attractant to target and regulate macrophage polarization. In this study, the opsonized nanoparticle (IgG/ [email protected] ) had several key features, including an immunoglobulin IgG (the opsonized layer), an anti-inflammatory agent berberine (Bb), and an oxidative stress-responsive bilirubin grafted polylysine biomaterial (BR-PLL) for drug loading (the inner nanocore). In vitro studies confirmed that IgG/ [email protected] prefer to be phagocytosed by M1 macrophage, not M0. And the internalized IgG/ [email protected] effectively promoted macrophage polarization toward the M2 phenotype and protected nearby chondrocytes. In vivo studies suggested that IgG/ [email protected] significantly enhanced therapeutic outcomes by suppressing inflammation and promoting cartilage repair while not prolonging the retention period compared to non-opsonized counterparts. This proof-of-concept study provided a novel opsonization trapping strategy for OA drug delivery and treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Haofeng Hu,Fulin Luo,Qian Zhang,Ming Xu,Xin Chen,Zhihao Liu,Haodong Xu,Lei Wang,Fei Ye,Kui Zhang,Bin Chen,Song Zheng,Jia Jin
In wound treatment, severe bleeding and infection are always primary challenges. Therefore, it is highly desired to develop novel dressing with both hemostatic and antibacterial capability. Herein, a series of biocomposite hemostatic films (BHFs) based alginate/chitosan/collagen-berberine have been prepared and well characterized for further biofunctional study. We have demonstrated that the hemostatic and antibacterial activities were significantly enhanced by calcium/berberine dual-crosslinking system in the film. Through the synergistic effects, BHF-6B exhibited a shorter in vivo clotting and wound healing time than that of commercial dressing in rat tail amputation and full-thickness skin defect models. Additionally, BHF-6B showed excellent bacteriostatic activity with long-term effects. Moreover, hemolysis and cytotoxicity tests in vitro illustrated the prominent biocompatibility of the composite films. Notably, BHF-6B could be degraded quickly and completely in vivo . Overall, the present work indicated that the functionalized BHF-6B has great potential as an absorbable biomaterial for wound treatment.
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LUMINESCENCE
·
2022
·Vol. 37
·期 7
·1078-1086
·
IF 2.613
Yingxin Chen,Xiaotong Huang,Lu Li,Junxian Wu,Yongqi Guo,Yachao Yao,Lihua Zhou
Coptisine (COP), one of the bioactive components in Rhizoma Coptidis, has many pharmacological effects. Meanwhile, the determination of COP is essential in pharmacological and clinical applications. Herein, we prepared carbon quantum dots (CQDs) by one-step oil-thermal method using paper mill sludge (PMS) as precursor, and developed a ratiometric fluorescence method for the determination of COP. The structural and optical properties of PMS-CQDs were evaluated through high-resolution transmission electron microscopy (HRTEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray powder diffraction (XRD), ultraviolet-visible (UV-vis), fluorescence, zeta potential and fluorescence lifetime experiments. Fluorescence intensity ratio at 550 nm and 425 nm ( I 550 / I 425 ) was recorded as an index for quantitative detection of COP. The detection concentration of COP ranges from 0.1 to 50 μM in good linear correlation ( R 2 = 0.9974) with a limit of detection of 0.028 μM (3 σ / k ). The quenching mechanism was deduced to be inner filter effect and static quenching. The ratiometric fluorescent probe showed impressive selectivity and sensitivity towards COP, and was successfully applied to the detection of COP in human urine with expected recoveries (95.22–111.00%) and relative standard deviations (0.46–2.95%), indicating that our developed method has a great application prospect in actual sample detection.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Jia-Man Liang,Fang Zhang,Yu-Lin Zhu,Xiao-Yan Deng,Xian-Ping Chen,Qiu-Ju Zhou,Ke-Jun Tan
Here, the high fluorescent silicon-doped carbon quantum dots (Si-CQDs) were prepared by a facile and one-pot hydrothermal assay using 3-aminopropyltrimethoxysilane as the carbon and silicon source. The prepared Si-CQDs exhibit favorable water-soluble, high-temperature resistance, acid resistance, alkali resistance, high ionic strength resistance, high photostability, film-forming ability and solid-state fluorescence. Compared to other Si-CQDs that have been reported, the prepared Si-CQDs show unique up-conversion fluorescence. Furthermore, it is found that berberine hydrochloride (BH) can effectively quench the down- and up-conversion fluorescence of the Si-CQDs, making it can be used as a highly sensitive and specific probe for BH dual-mode sensing. Meanwhile, the linear range of down-conversion fluorescence detection for BH is 0.5-30.0 µmol/L with a limit of detection (LOD) of 50 nmol/L, and the linear range of up-conversion fluorescence assay for BH is 0-25.0 µmol/L. The mechanism of down-conversion fluorescence quenching by BH was investigated through a series of studies. The results show the quenching mechanism is the inner filter effect (IFE). Moreover, this proposed strategy has been well used to analyze BH in urine samples with satisfactory results.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Xingxing Feng,Qi Xie,Hongbo Xu,Tiantian Zhang,Xiaonan Li,Yinmei Tian,Hongbing Lan,Li Kong,Zhiping Zhang
The common and frequent disease, ulcerative colitis (UC), causes serious physical and mental distress to patients. M2 macrophages have proven to play a role in anti-inflammation, which is a new potential target for UC therapy. In this study, we designed a safe and macrophages-targeting oral drug delivery system. Natural products, berberine (BBR), and Epigallocatechin Gallate (EGCG) with anti-inflammatory activity were assembled and encapsulated into yeast microcapsule (YM), generating therapeutic system BBR/MPN@YM. BBR and EGCG exhibited synergistic effects against UC through the effect of antioxidation. Through the interaction between β-1,3-d-glucan on the surface of YM and dectin-1 receptors on macrophages, BBR/MPN@YM could be effectively transported to inflammation parts and internalized into macrophages, avoiding gastric degradation. In the in vivo UC mouse model, BBR/MPN@YM could transform M1 macrophages into anti-inflammatory M2 macrophages, thus exerting specific anti-inflammatory effects. Therefore, this BBR/MPN@YM targeted oral drug delivery system provided a new macrophages-targeting strategy for the clinical treatment of UC.
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