International Journal of Analytical Chemistry
·
2015
·
IF 1
Nai-Dong Chen,Nai-Fu Chen,Jun Li,Cai-Yun Cao,Jin-Mei Wang,He-Ping Huang
GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed.
研究领域:
植物化学
·
药用植物学
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DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2021
·Vol. 21
·期 12
·6088-6093
·
IF 0
Bai Wenxia,Zhou Jinxuan,Shi Xueting,Wang Haitao,Liu Yanhua,Feng Libang
The widespread application of artificial superhydrophobic material is hindered by the poor durability and regeneration of artificial superhydrophobicity. The problem is expected to be resolved by endowing the superhydrophobic material with self-similarity. Herein, Copper-based superhydrophobic
material intensified by furfuryl alcohol resin (Cu/FAR) with long-term durability and high strength is developed, and the obtained Cu/FAR composite reveals excellent and durable superhydrophobicity. Moreover, it is a remarkable fact that the as-prepared superhydrophobic Cu/FAR exhibits outstanding
durability and maintenance of superhydrophobicity grounded on the good self-similarity in micro-structure, chemical structure and composition both externally and internally. Consequently, the superhydrophobicity can be maintained or regenerated without difficulty even if superhydrophobic surface
has been damaged or fouled accidentally. Therefore, the method provides a new thought to prepare superhydrophobic material with robust durability and outstanding maintenance.
研究领域:
材料科学
·
表面化学
✓ 已验证
DOI
RSC Advances
·
2018
·Vol. 8
·期 30
·16574-16584
·
IF 2.936
Hong-Xun Wang,Yang Yi,Jie Sun,Olusola Lamikanra,Ting Min
Thirty-nine polysaccharides isolated from different parts of 13 lotus root varieties were characterized with fingerprint and chemometrics analyses to explore their similarity and diversity. The physicochemical features of lotus root polysaccharides (LRPs) were found to be the following: LRPs contained mainly polysaccharides (5.94 kDa) and polysaccharide-protein complexes (11.57 kDa and 5.30 kDa); their carbohydrates were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose approximately in the molar ratio of 0.19 : 0.14 : 0.08 : 0.17 : 6.49 : 1.00 : 0.16; and node LRPs possessed more binding proteins and uronic acids than both flesh and peel LRPs. Their fingerprints based on Fourier-transform infrared spectroscopy, pre-column derivatization high-performance liquid chromatography and high performance size-exclusion chromatography all exhibited relatively high similarities, contributing to the common figerprint models which could be utilized as references for the identification of LPRs. In addition, the fingerprint characteristics associated with the between-group variability of LRPs in the score plots derived from multivariate analytical models might indicate which variety or part of lotus root they were isolated from. Therefore, multi-fingerprinting techniques have the potential to be applied to the identification and quality control of LRPs.
研究领域:
植物化学
·
食品科学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Yinan Liu,Chunying Zhu,Taotao Fu,Xiqun Gao,Youguang Ma
In ATPS, the ultra-low interfacial tension makes the stable generation of droplets very difficult. In this paper, the stable and homogeneous preparation of ATPS droplets is achieved over a wide range of flow rates, and the self-similarity analysis and formation dynamics of the droplets are studied. Formation of ATPS droplets show good self-similarity. The generation process of ATPS droplets can be divided into slow squeezing, fast squeezing and rapid pinch-off stages. In the slow squeezing stage, the dimensionless minimum neck width w m / w c is linear with the time, and the absolute value of the slope increases with the capillary number of continuous phase Ca c . In the rapid squeezing and rapid pinch-off stages, the w m / w c satisfies the power-law relationship with time, and the absolute value of the power-law indexes decrease with increasing Ca c , and the pre-exponential factor of pinch-off stage increases with increasing Ca c .
研究领域:
生物工程
·
流体力学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Pengshuai Zhang,Haiyan Luo,Lan Cui,Jixiu Deng,Shiyu Xie,Danfeng Liu,Shuqi Wang,Xiaoying Si,Zichao Wang,Yameng Wan,En Zhang,Xing Li,Lu Zhang
Solid-liquid equilibrium solubility of artemisinin (ART) Form I in twelve mono-solvents namely N, N-dimethylformamide (DMF), oxolane (THF), n -propanol (NPA), 4-methylpentan-2-one (MIBK), ethyl formate (EF), butyl acetate (BAC), 2-ethoxyethanol (EGEE), 1,4-Dioxane, 2-methoxyethanol (EGME), 2-propoxyethanol (EGPE), propyl acetate (NPAC) and 2-butoxyethanol (EGBE) from 283.15 K to 323.15 K were determined by a laser dynamic method under 0.1 MPa. The minimum solubility of ART (Form I) was found in NPA at 283.15 K (0.004541), the maximum mole fraction solubility of ART (Form I) was observed as 0.2523 in DMF at 323.15 K. At the temperature of 298.15 K, the experimental mole fraction solubility order of ART Form I in the tested solvents was DMF (0.1559) > 1,4-Dioxane (0.09117) > THF (0.08301) > MIBK (0.05054) > EF (0.04902) > NPAC (0.03860) > BAC (0.03658) > EGME (0.02031) > EGEE (0.01859) > EGPE (0.01817) > EGBE (0.01689) > NPA (0.007185). The solubility behavior was computationally correlated by Margules model, Wilson model, NRTL model, NRTL-SAC model, Buchowski-Ksiazczak λh model and UNIQUAC model, the total RMSD was no>0.025 in the selected models. With the help of Hansen solubility parameters (HSPs) analysis, molecular similarities of ART (Form I) in twelve mono-solvents were investigated. Besides, Hirschfeld surface analysis (HSs) was considered to evaluate the intermolecular interaction of artemisinin. Moreover, thermodynamic properties including Δ mix H , Δ mix G , Δ mix S , lnγ 1 , l n γ 1 ∞ of ART (Form I) in the selected solvents were estimated. All the negative Δ mix G and positive Δ mix S values indicated that the mixing process were spontaneous and entropy-increasing.
研究领域:
药学
·
物理化学
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产品关联
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Qian Hui,Wang Yuxuan,Zhou Xibin,Gu Tao,Wang Hui,Lyu Hao,Li Zhikai,Li Xiuxu,Zhou Huan,Guo Chengchen,Yuan Fajie,Wang Yajie
The UniProt database is a valuable resource for biocatalyst discovery, yet predicting enzymatic functions remains challenging, especially for low-similarity sequences. Identifying superior enzymes with enhanced catalytic properties is even harder. To overcome these challenges, we develop ESM-Ezy, an enzyme mining strategy leveraging the ESM-1b protein language model and similarity calculations in semantic space. Using ESM-Ezy, we identify novel multicopper oxidases (MCOs) with superior catalytic properties, achieving a 44% success rate in outperforming query enzymes (QEs) in at least one property, including catalytic efficiency, heat and organic solvent tolerance, and pH stability. Notably, 51% of the MCOs excel in environmental remediation applications, and some exhibited unique structural motifs and unique active centers enhancing their functions. Beyond MCOs, 40% of L-asparaginases identified show higher specific activity and catalytic efficiency than QEs. ESM-Ezy thus provides a promising approach for discovering high-performance biocatalysts with low sequence similarity, accelerating enzyme discovery for industrial applications.
研究领域:
生物信息学
·
酶工程
·
工业微生物学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-14
·
IF 4.122
Zhu Xiurui,Su Shisheng,Fu Mingzhu,Liu Junyuan,Zhu Lingxiang,Yang Wenjun,Jing Gaoshan,Guo Yong
Droplet microfluidics has attracted significant interests in functional microcapsule synthesis, pharmaceuticals, fine chemicals, cosmetics and biomedical research. The low variability of performing chemical reactions inside droplets could benefit from improved homogeneity and reproducibility. Therefore, accurate and convenient methods are needed to monitor dynamic droplet generation processes. Here, a novel Cosine Similarity Algorithm (CSA) method was developed to monitor the droplet generation frequency accurately and rapidly. With a microscopic droplet generation video clip captured with a high-speed camera, droplet generation frequency can be computed accurately by calculating the cosine similarities between the frames in the video clip. Four kinds of dynamic droplet generation processes were investigated including (1) a stable condition in a single microfluidic channel, (2) a stable condition in multiple microfluidic channels, (3) a single microfluidic channel with artificial disturbances, and (4) microgel fabrication with or without artificial disturbances. For a video clip with 5,000 frames and a spatial resolution of 512 × 62 pixels, droplet generation frequency up to 4,707.9 Hz can be calculated in less than 1.70 s with an absolute relative calculation error less than 0.08%. Artificial disturbances in droplet generation processes can be precisely determined using the CSA method. This highly effective CSA method could be a powerful tool for further promoting the research of droplet microfluidics.
研究领域:
化学工程
·
生物医学工程
·
微流体技术
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产品关联
DOI
PubMed
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2019
·
IF 3.319
Qi Yao,Hongbing Wang,Yunzhuo Wang,Lintao Zhang,Wanlu Zhang,Yi Zhang
In this work, we synthesized a sun-like light source with tri-chromatic broad spectra, composed of light source units with single-peak 435-nm carbon quantum dots (QDs) and 695-nm perovskite QDs, and dual-peak 510- and 590-nm Ag-doped InP QDs, referencing CIE standard illuminants D50 and D65. Based on a spectral angle measure approach, we adopt the spectral similarity index (SSI) to measure the similarity between the synthesized light source and reference light source. We quantify the colour rendering and SSI of the tri-chromatic light source units and obtain the colour rendering and SSI as a function of chromaticity coordinate. The synthesized light source reaches an ideal performance with a maximum spectral similarity of 0.98 and colour rendering of about 93. This work is the first to synthesize a full-spectrum sun-like source with three QD light units, including a dual-peak QD unit, and proves QD LEDs as promising light sources for synthesizing full-spectrum light sources. In the future, improving the long wavelength part (>700 nm) of the broad-spectrum QD LED unit may significantly improve the colour rendering property.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Chuanfei Yu,Feng Zhang,Gangling Xu,Gang Wu,Wenbo Wang,Chunyu Liu,Zhihao Fu,Meng Li,Sha Guo,Xiaojuan Yu,Lan Wang
BVZ-BC (bevacizumab-biosimilar candidate) is a proposed biosimilar to bevacizumab. Bevacizumab binds to vascular endothelial growth factor (VEGF) type A and prevents the interaction of VEGF with its receptors on the surface of endothelial cells, neutralizing angiogenesis required for the growth, persistence, and metastases of solid tumors. An analytical comparison of BVZ-BC and bevacizumab was performed using state-of-the-art analytical techniques, including biochemical and biophysical characterization, biological activity, and immunological properties. Multiple attributes of the molecules were evaluated, including amino acid sequence, disulfide structure, glycan profiles, free thiol content, isoelectric point, protein content, subvisible particles, higher-order structure such as near- and far-ultraviolet circular dichroism and differential scanning calorimetry, product purity and product-related impurities, and process-related impurities. Biological activity assessment employed orthogonal assays such as the VEGF cell-based bioassay and the VEGF enzyme-linked immunosorbent assay, and Fc functional assays to interrogate all expected biological activities. An accumulation of 18 batches of bevacizumab (sourced in China, manufactured in Europe, Roche) and 10 batches of BVZ-BC representing unique drug product lots from each individual drug substance lot were utilized in this study. The analytical similarity between BVZ-BC and bevacizumab was assessed and demonstrated the similarities of all of the quality attributes between BVZ-BC and bevacizumab.
研究领域:
肿瘤学
·
药物开发
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2023
·Vol. 35
·期 47
·2302973
·
IF 29.4
Dongjin Xie,Mei Zu,Mingyang Li,Dongqing Liu,Zi Wang,Qingwen Li,Haifeng Cheng
The unmet spectral mimicry of foliar green in camouflage materials is hampered by the lack of colorants with similar spectral properties to chlorophyll, resulting in substantial risks of exposure from hyperspectral target detection. By drawing inspiration from leaf chromogenesis, a microcapsule colorant with a chloroplast-like structure and chlorophyll-like absorption is developed, and a generic bilayer coating is designed to provide high spectral similarity to leaves with different growth stages, seasons, and species. Specifically, the microcapsule colorant preserves the monomeric absorption of the internal phthalocyanine and features the manufacturability of conventional pigments, such as amenability to painting and patterning, and compatibility to different substrates. The pigmented artificial leaves successfully deceive the hyperspectral classification algorithm in a foliar background, and outperforming the state-of-art spectral simulation materials. This coloration strategy expands the knowledge base of the spectral fine tuning of composite colorants, which are essential for their application in spectral-resolved optical materials.
研究领域:
材料科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IUCrJ
·
2021
·
IF 4.769
Jiawei Lin,Peng Shi,Ying Wang,Lingyu Wang,Yiming Ma,Fei Liu,Songgu Wu,Junbo Gong
Template design on polymorph control, especially conformational polymorphs, is still in its infancy and the result of polymorph control is often accidental. A method of regulating the crystallization of conformational polymorphs based on the crystal structure similarity of templates and the target crystal form has been developed. Crystal structure similarity was considered to be able to introduce lattice matching (geometric term) with chemical interactions to regulate conformational polymorph nucleation. The method was successfully applied to induce the crystallization of DA7-II [HOOC–(CH 2 ) n −2 –COOH (diacids), named DA n , where n = 7, 9, 15, 17 and II represents the metastable polymorph] on the surface of DA15-II. An analogous two-dimensional plane – the (002) face of both DA15-II and DA7-II – was firstly predicted as the epitaxially attached face with similar lattice parameters and the strongest adsorption energy. The powder DA15-II template with the preferred orientation face in (002) presented much stronger inducing DA7-II ability than the template with other preferred orientation faces. The epitaxial growth of DA7-II on DA15-II through an identical (002) face was clearly observed and verified by the single-crystal inducing experiments. The molecular dynamics simulation results demonstrated that the strong interactions occurred between DA7 molecules and the (002) face of DA15-II. This method has been verified and further applied to the crystallization of DA7-II on the surface of DA17-II and DA9-II on the surface of DA15-II. This study developed a strategy based on structure similarity to regulate the conformational polymorph and verified the significant role of lattice matching and chemical effects on the design and preparation of templates.
研究领域:
材料科学
·
结晶学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 17
·10338-10351
·
IF 3.119
Libing Chen,Fang Zhao,Wenzhu Li,Zeqi Chen,Jianyang Pan,Difeifei Xiong,Bailing Li,Qingjie Zhang,Haibin Qu
This paper evaluates a multiple and global analytical indicator of batch consistency in traditional Chinese medicine injections (TCMIs) via a chemometrics tool, which is more comprehensive to appraise quality consistency of different batches of injections than the traditional method of fingerprint similarity. A commonly used TCMI, Salviae miltiorrhizae and ligustrazine hydrochloride injection (SLI), was employed as a model. With the aid of a chemometrics tool (principal component analysis, PCA), evaluation of multiple and global analytical indicators of batch consistency, which included saccharides, phenolic acids and inorganic salts (18 indicators in total), was carried out to appraise the quality consistency of 13 batches of injection provided by the Guizhou Baite Pharmaceutical Co., Ltd. (Guizhou, China). Compared with the traditional HPLC-UV fingerprint similarity evaluation, the method proposed in the paper can more comprehensively and correctly reflect the quality consistency of different batches of injections. In this paper, the multi-index evaluation result showed poor batch consistency, which was more consistent with the determination results, while the fingerprint similarity evaluation results still showed good batch consistency. The HPLC-UV fingerprint reflects only substances with UV absorption, but it is not able to reflect substances without UV absorption or weak UV absorption, which leads to inappropriate conclusions. Therefore, quality consistency of injections can be effectively appraised by evaluation of multiple and global analytical indicators, instead of HPLC-UV fingerprint only. For visualizing the batch consistency of the multiple and global analytical indicators, a heat map was used to represent the fluctuation. Furthermore, critical indicator identification was also applied to select several indicators that should be paid more attention during the process of quality control of injection. And the analysis result showed that Na+, fructose (Fru), glucose (Glc), manninotriose (Man), danshensu (DSS) and salvianolic acid B (SAB) are the indicators that should be given more attention when controlling the quality of injections, also called critical quality control indicators. The proposed method provides a reference for the quality control of TCMIs and has broad application potential.
研究领域:
中药学
·
分析化学
·
制药工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）