ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2019
·Vol. 27
·期 2
·2273-2286
·
IF 2.914
Chen Leilei,Li Haipu,Liu Yang,Cui Yue,Li Yue,Yang Zhaoguang
In view of the increasing attention on antibiotic contamination and their scarce data in the inland river (especially for the sediment), the occurrence of 28 antibiotics in sediments from the Xiangjiang River was comprehensively analyzed, and 22 antibiotics were detected with a total concentration ranging from 4.07 to 2090 ng g −1 . The residue was almost at a moderate or higher level in the aquatic environment around the world. Fluoroquinolones and tetracyclines were the dominant detected antibiotics, and the maximum total concentration could reach to 2085 ng g −1 , though that in surface water was just 33.4 ng L −1 . Oxytetracycline and chlortetracycline could be detected with high concentration in areas with lower population density. Usage profile of each antibiotic may be responsible for the spatial variation. Principal component analysis-multiple linear regression model indicated that direct discharge of domestic wastewater and livestock or aquaculture sewage could contribute 94.2% of the pollution. Redundancy analysis was used to screen out the environment variables, which were closely related to the pseudo-partitioning coefficients (K d ) of antibiotics in sediment and surface water for the first time, and showed that the K d was correlated with sediment pH negatively and organic carbon, total phosphorus, and conductivity of the sediments positively. High sedimentary organic carbon was considered to promote the higher K d in this river. This study would deepen the understanding of the occurrence of antibiotics in sediments from the inland rivers and provide scientific support for controlling the antibiotic contamination.
研究领域:
环境科学
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生态学
·
公共卫生
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2020
·Vol. 44
·期 10
·e14824
·
IF 1.405
Wenwei Chen,Zhenbao Jia,Guangrong Huang,Yao Hong
Marine algae are important sources of biological substances. Polysaccharides and oil in algae have a broad range of biological activities. The aim of our study was to develop a novel method for the extraction of polysaccharides and oil from Schizochytrium limacinum by enzyme-assisted three-phase partitioning. S. limacinum was pretreated by protamex hydrolysis for 2 hr, at pH 3.0, temperature of 45°C, and enzyme concentration of 2.0%. Response surface was used to analyze the three-phase extraction and Box–Behnken design was employed to model algae oil and polysaccharides. The following optimal conditions were obtained by global optimization: 34% mass fraction of ammonium sulfate, 1:2.4 ratio of slurry to t -butanol, 41 min time, 40°C temperature, 35.69% oil yield, and 5.16% polysaccharides yield. The polysaccharides exhibited potential antioxidant activity in DPPH, ABTS, and reducibility assay. The results indicated that the algal polysaccharides may be used as a natural antioxidant in food. Practical applications The application on Schizochytrium limacinum is mainly to extract DHA, but the algal polysaccharide has not been used. Algae oil and polysaccharides are simultaneously extracted from S. limacinum by enzyme-assisted three-phase partitioning (EATPP). The results suggest the EATPP is a novel method to obtain simultaneously oil and polysaccharides.
研究领域:
海洋生物学
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生物化学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Huadong Tan,Qiao Xing,Ling Mo,Chunyuan Wu,Xiaoying Zhang,Xiaoyu He,Yuefu Liang,Rong Hao
Rice–vegetable rotation practices prevail in subtropical and tropical agriculture worldwide, with applications of current-use herbicides (CUHs) vital for nontarget plant control. After application, CUHs migrate to environmental compartments, where the occurrence, fate, and ecological risks have not been well characterized. To further understand the occurrence and multiphase partitioning, as well as to evaluate potential drivers and mixture risks in environmental compartments, we analyzed 11 CUHs in 576 samples from 36 rice–vegetable rotations in Nandu River basin, Hainan, China. Samples included soil, water, suspended particulate matter , and sediment collected during both rice and vegetable planting periods. The CUH concentrations varied across environmental compartments, but with high levels of glyphosate and aminomethylphosphonic acid organophosphorus herbicides (OPHs) frequently detected, accounting for 82.3 % to 99.0 % in environmental compartments. Phenoxy acid (PAA) and chloroacetanilide (ANH) herbicides were detected at lower frequencies. Spatiotemporal variation was significantly different among OPHs, ANHs, and PAAs, with geographic and crop-related patterns most evident for CUHs rather than OPHs. Structural equation model, redundancy, and boosted regression tree analyses indicated environmental compartment properties (pH, organic matter, and Fe/Al oxides), crop type, and wet/dry climate were important drivers of spatiotemporal patterns. Fugacity ratios indicated multiphase partitioning and transport of CUHs differed in rice and vegetable planting periods. A new assessment framework based on species-sensitive distributions and environmental compartment weight index indicated unacceptable risks of CUHs (risk quotient >1 in >50 % of sites), with most risks from OPHs (10.5 % to 98.0 %) and butachlor , acetochlor , and 2,4-dichlorophenoxyacetic acid. Risk hot spots were identified as the soil, the central region, and the vegetable planting period, potentially threatening nontarget organisms (e.g., Lemna minor , Glomus intraradices , and Apis mellifera ). This study provides a new risk assessment framework and demonstrates the domination of OPHs in CUH contamination and risks in the tropics, thus helping guide policymakers and stakeholders on herbicide management.
研究领域:
环境科学
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生态学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2024
·
IF 8.7
Zhongnan Wu,Chong Li,Junhao Li,Tanggan Wang,Meifeng Li,Leyi Zhao,Huimei Ye,Jiaheng Chen,Jiajia Zan,Lijun Song,Qian Zhang,Shaojie Zhang
Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t -butanol’s environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t -butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs). The optimized DES-4, composed of lauric acid and nonanoic acid (molar ratio = 1:1), achieved a 35.28 % extraction yield under specific conditions and can be recycled five times with minimal yield loss. AGP-DES-4 has a broad molecular weight distribution (2.48–174.64 kDa) and mainly consists of mannose, glucose, galactose, and arabinose. In vivo Drosophila models show that AGP-DES-4 improves UC fly survival and enhances intestinal barrier function by regulating the proliferation and differentiation of intestinal epithelial cells (IECs) and intestinal stem cells (ISCs). Our results highlight the effectiveness of the AGP-DES-4 extraction method and its potential therapeutic value for treating UC.
研究领域:
生物化学
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药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Yuxiao Hu,Xueying Chen,Zhijian Tan
Ammonium sulfate and t -butanol are widely used in conventional three-phase partitioning (TPP); however, their use is not eco-friendly. In this study, a novel TPP was developed using pH-responsive deep eutectic solvents (DESs) and sugars as substitutes for t -butanol and ammonium sulfate, respectively. This TPP was further used for the separation and purification of radish ( Raphanus sativus L.) peroxidase (POD). DES-3 (dodecanoic acid: decanoic acid = 1:1) and sucrose were selected as optimal phase-forming components. Single-factor experiments were performed to investigate the factors influencing the TPP process, and three major factors (sucrose concentration, extraction time, and extraction temperature) were optimized by response surface methodology (RSM). Under the optimized conditions, the POD recovery and purification fold reached 101.66% and 11.56, respectively. Cyclic experiments confirmed the retrievability of DESs. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the novel TPP had better purification efficiency than the traditional TPP. The method proposed in this study is more efficient than conventional TPP, and it does not require the use of organic solvents and high concentration salt solutions. Therefore, this method is promising in the application of TPP.
研究领域:
生物化学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yubing Dai,Jiaxin Liu,Naibing Liang,Zhangpeng Guo,Jing Jin,Congjie Bi,Haijun Zhang,Ningbo Geng,Jiping Chen
Plasma and blood cells may exist different phase partitioning of elements due to their distinct properties and functions, while the distribution patterns of other elements across plasma, platelets, leukocytes, and erythrocytes remain largely unexplored. This study developed a method to rapidly separate these fractions of human blood and quantify 22 elements in each fraction. Notably, elements like Fe, Zn, and Rb were higher in erythrocytes, whereas Cd, Cr, V, and Sr were concentrated in leukocytes. Most metals detected in plasma account for < 40 % of the total concentration in blood, particularly toxic metals, with Cr, As, Cd, Hg, and Pb accounting for 63–75 % in blood cells, suggesting plasma-based assessments may underestimate actual exposure. Metal concentrations in plasma were positively correlated with those in whole blood ( ρ ≥ 0.48), except for Fe ( ρ = 0.13). We derived empirical ratios to estimate the internal exposure levels of elements from plasma to blood. Finally, we prioritized the assessment of potential acute and non-cancer risks associated with five toxic metals. As evidenced by PrHQ values, the toxicity level of As were the highest. Notably, the PrHQ values for these toxic metals in whole blood were approximately three times higher than those detected in plasma.
研究领域:
环境毒理学
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分析化学
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金属组学
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公共卫生
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环境科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Changyang Qiu,Zhihong Chen,Fenjuan Hu,Hailing Wu,Lu Wang,Meixia Liu,Kai Zhou
The seed cake of Camellia oleifera Abel. (SCOC), a big residue produced after Camellia oil extraction, enriches with many bioactive components. However, it is often discarded directly due to the lack of cost-effective extraction. Herein, a three-phase partitioning (TPP) system is first time tried for the simultaneous extraction of three major ingredients i.e. residual oil, protein, and polysaccharide from SCOC. Results from the extraction yields show that the established method is more efficient or at least comparable to conventional techniques. The developed method takes only 1.5 h and can be carried out at mild temperature of 40 °C, making it a cost-effective method. The successful scaling up of the extraction system at a 30 L extraction tank confirms its scalability. Furthermore, compared with conventional extraction methods, the TPP extracts shows better product quality. These facts, along with simultaneous extraction features of the method, allow us to use TPP as a promising extraction technology of SCOC biomolecules.
研究领域:
生物技术
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食品科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shaojie Zhang,Zhongnan Wu,Qian Zhang,Han Zhao,Junhao Li
This study explores the extraction of polysaccharides from Nostoc commune Vauch. using ultrasonic-assisted three-phase partitioning with deep eutectic solvents (UA-TPP-DES). Response surface methodology was used to determine the optimized UA-TPP-DES conditions as follows: a 1: 2 M ratio of lauric acid to terpineol, 30 min of ultrasonication at 60 °C with 100 W power, 20 % moisture content, 20 % w/w (NH 4 ) 2 SO 4 concentration, and a 2: 1 top-to-bottom phase volume ratio. The UA-TPP-DES method significantly improved the polysaccharide extraction efficiency of N. commune (yielding NCP-DES-3, 7.31 %) compared to conventional water extraction (3.35 %). The structural features of NCP-DES-3 were characterized through various physical and chemical methods, including molecular weight determination, monosaccharide composition analysis, Fourier Transform Infrared Spectroscopy (FT–IR), Gas Chromatography-Mass Spectrometry (GC–MS), and Nuclear Magnetic Resonance (NMR) spectroscopy. Additionally, the dextran sulfate sodium (DSS)-induced ulcerative colitis model in Drosophila was used to study the anti-ulcerative colitis (UC) activity of NCP-DES-3. In vivo experiments showed that NCP-DES-3 improved the intestinal barrier by modulating intestinal epithelial cell (IEC) and intestinal stem cell (ISC) growth and differentiation. Our results demonstrated that the UA-TPP-DES approach is an eco-friendly and efficient strategy for polysaccharide extraction, while the bioactive polysaccharide NCP-DES-3 shows promising potential as a natural therapeutic agent for UC.
研究领域:
生物技术
·
植物化学
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食品科学
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胃肠病学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xueying Chen,Changyong Cai,Zhijian Tan
T -butanol is widely used in three-phase partitioning (TPP). This study used deep eutectic solvents (DESs) to substitute t -butanol for tomato peroxidase (POD) purification. DES-5 (menthol and octanoic acid) was screened as the optimal solvent. The extraction process was optimized using single-factor experiments. Thereafter, the major three factors were optimized by response surface methodology (RSM). When the ammonium sulfate ((NH 4 ) 2 SO 4 ) concentration was 42%, DES: crude extract (v/v) was 2:1, pH was 5.7, and extraction temperature was 30 °C, the recovery and purification fold reached a maximum of 104.71% and 9.76, respectively. The obtained tomato POD was analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), which showed that this system could effectively purify POD. Finally, recycling studies indicated the good cycle stability of the DES. This TPP based on DESs is greener and more efficient, indicating that DESs can be good alternative solvents for further applications of TPP.
研究领域:
生物技术
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xueying Chen,Yuxiao Hu,Zhijian Tan
Traditional three-phase partitioning (TPP) systems used for extracting and purifying bioactive substances typically consist of t -butanol and ammonium sulfate. However, the use of volatile and flammable organic solvents and high-concentration inorganic salts results in environmental pollution. Therefore, an innovative TPP based on deep eutectic solvents (DESs) and sugars (sugaring-out effect) was used to extract and preliminarily purify cucumber peroxidase (POD). Various factors affecting the extraction were optimized using response surface methodology (RSM). DES-8 (menthol: octanoic acid = 1:1) and sucrose were selected as the optimal constituents of TPP. Under these conditions, the sucrose concentration was 36% (w/v), DES: crude extract (v/v) was 1.4:1, system pH was not adjusted, extraction time was 6 min, and extraction temperature was 31 °C; the enzyme recovery and purification fold reached 84.53% and 5.28, respectively. Recycling experiments demonstrated that the DESs can be well recycled and reused after five recycles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze cucumber POD, which showed that the TPP system achieved primary purification of cucumber POD. This TPP is much greener and more efficient than traditional TPP systems and shows great application potential in the enzymes and proteins separation field.
研究领域:
生物化学
·
生物技术
·
环境科学
·
酶学
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Yanfang Fu,Yajiao Hong,Shaojie Zhang,Jiaheng Chen,Guorong Wu,Guixiang Wang,Qian Zhang
Ultrasound-assisted extraction of Cissus repens polysaccharides (CRPs) was optimized through response surface methodology (RSM) based on Box–Behnken design (BBD). The maximum CRPs yield (16.18%) was achieved under the optimum extraction conditions: extraction time 72 min, extraction temperature 74 ℃, extraction power 240 W. Then three-phase partitioning (TPP) method combined with gradient alcohol precipitation was used to obtained CRP20, CRP40, CRP60 and CRP80 from CRPs, and CRP80 has a higher purity than others. The primary chemical and structural characteristics of CRP80 were investigated by UV, FT-IR, high-performance liquid chromatography (HPLC) and high-performance gel-permeation chromatography (HPGPC). CRP80 is mainly composed of glucose, galactose, arabinose and mannose, with a molecular weights of approximately 2.95 kDa. Furthermore, the antioxidant activity and hypoglyceamic activity of CRP80 in vitro were evaluated. The results showed that CRP80 had strong scavenging activities on ABTS, hydroxyl and DPPH radicals, as well as high scavenging activities on α-glucosidase and α-amylase. Our research provided an efficient method for the extraction of polysaccharides from C. repens and CRP80 has potential as a promising source of natural antioxidants and hypoglycemic agent for the functional food and medicinal industries.
研究领域:
药学
·
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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