COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Qin Zhang,Yunhui Liu,Kevin C. Chen,Gonggen Zhang,Xuhua Shi,Hanjia Chen
A macromolecular biocompatible surface modifier for polyurethane (PU), namely, polyurethane-block-poly(vinylpyrrolidone) (PU- b -PVP) copolymer, was synthesized by coupling hydroxyl-terminated PVP with diisocyanate-terminated poly(ethylene glycol) using 1,6-hexamethylene diisocyanate (HDI) as coupling agent. The chemical structure and molecular weight of the synthesized copolymer were systematically characterized by nuclear magnetic resonance spectrum ( 1 H NMR), Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). The synthesized surface modifier was blended with PU and effectively increased the hydrophilicity of PU. To evaluate the hemocompatibility of the PU/PU- b -PVP blend films, the extent of platelet adsorption, plasma recalcification time and hemolysis were measured. To determine if the blend films were toxic to mammalian cells, viability of the cells cultured on the blend films was assayed. All results indicated that the blood and cell compatibilities of the PU films were improved significantly after entrapment of a few PU- b -PVP copolymers into PU.
研究领域:
材料科学
·
生物材料
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2015
·Vol. 22
·期 5
·1-12
·
IF 1.92
Zhang Qin,Liao Jian-Fang,Shi Xu-Hua,Qiu Yong-Ge,Chen Han-Jia
A macromolecular biocompatible surface modifier for polyurethane (PU), namely, polyurethane-block-poly (chloromethyl styrene) (PU- b -PCMS) copolymer, was synthesized by coupling hydroxyl-terminated PCMS with diisocyanate-terminated PU and further quaternizing. The synthesized surface modifier was blended with PU and heparin was absorbed on PU surface by ion exchange. The modification effectively increased the hydrophilicity and water absorption of PU. To evaluate the hemocompatibility of the modified blend films, the extent of platelet adsorption, plasma recalcification time and hemolysis were measured. To determine if the blend films were toxic to mammalian cells, viability of the cells cultured on the blend films was assayed. All results indicated that the blood and cell compatibilities of the PU films were improved significantly after entrapment of a few quaternized PU- b -PCMS copolymers into PU and further heparinizing.
研究领域:
材料科学
·
生物医学工程
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2019
·
IF 0
Jinfeng Lei,Xinying Li,Shen Wang,Lun Yuan,Liming Ge,Defu Li,Changdao Mu
The self-healing hydrogels are extremely attractive in biological and biomedical fields. The imine bond obtained by the Schiff base reaction is a commonly used dynamic covalent bond to fabricate self-healing hydrogels. Gelatin is a commonly used natural macromolecule in the biomedical field with excellent biocompatibility, biodegradability, and nonimmunogenicity. However, the gelatin-based hydrogels with self-healing ability are rarely reported based on imine bonds. Herein, we present a facile approach to fabricate a gelatin hydrogel with self-healing ability based on the Schiff base reaction. The gelatin was first reacted with ethylenediamine to increase the content of amino groups. Then dialdehyde carboxymethyl cellulose was used to cross-link amino-gelatin to fabricate the self-healing hydrogel. The results showed that the fabricated hydrogel exhibited good self-healing ability as expected because of the formed dynamic imine bonds between amino-gelatin and dialdehyde carboxymethyl cellulose. The hydrogel also presented good fatigue resistance and self-recovery capacity. Moreover, the self-healing hydrogel possessed ideal hemocompatibility and cytocompatibility. In sum, the fabricated self-healing hydrogel has application prospects in biomedical fields, such as injectable cell and drug carrier and injectable tissue engineering scaffold.
研究领域:
生物工程
·
生物医用材料
·
组织工程
✓ 已验证
产品关联
DOI
Biomedical Materials
·
2014
·Vol. 9
·期 1
·015007
·
IF 2.922
Qi Wang,Pei Liu,Peifeng Liu,Tao Gong,Suming Li,Yourong Duan,Zhirong Zhang
Biodegradable chitosan-graft-polylactide (PLA–CS) copolymers were prepared by the grafting of a poly(L-lactide) (PLLA) or poly(D-lactide) (PDLA) precursor to the backbone of chitosan using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC ⋅ HCl) and N-hydroxysuccinimide (NHS) as a coupling agent. The blood and cell compatibility of the graft copolymers were investigated in comparison to PLLA and PDLA homopolymers. The coagulation properties of PLA–CS were evaluated by hemolysis, plasma recalcification, dynamic blood clotting and protein absorption assays. PLA–CS copolymers present similar hemolysis ratio and plasma recalcification time as PLA, but slower dynamic blood clotting and lower protein absorption. The cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), agar diffusion and lactate dehydrogenase (LDH) experiments. All the samples presented no effect on the viability to cells. Inflammatory cytokine analysis using sandwich ELISAs revealed that PLA–CS would not stimulate inflammatory activity.
研究领域:
生物材料学
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2017
·Vol. 74
·期 9
·3797-3818
·
IF 1.43
Wang Chen,Wang Qian,Li Shuangsi,Zhang Man,Zhao Weifeng,Sun Shudong,Zhao Changsheng
In this study, polyethersulfone (PES) membranes were modified by in situ cross-linking polymerization of polyurethane with functional groups of –COOH and –SO 3 H using PEG as the chain extender in one pot, followed with a phase inversion technique. The composite membranes were characterized by FTIR, water contact angle, and scanning electron microscope. Comparing with pristine PES membrane, the modified membranes showed decreased water contact angles and changed cross-sectional morphology. The modified membranes also showed decreased protein adsorption and suppressed platelet adhesion, as well as improved antifouling property. In addition, the composite membranes had prolonged activated partial thromboplastin times due to the functional groups and improved hydrophilicity. The results of contact activation and complement activation also indicated that the modified membranes had good blood compatibility. Furthermore, the cytocompatibility was significantly improved. The highly hemocompatible PES composite membranes fabricated by the in situ cross-linking polymerization of polyurethane may have great potential to be used in the field of blood purification.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2016
·
IF 1.733
Peng Wang,Pengzheng Liu,Haitao Peng,Xiaoman Luo,Huipin Yuan,Juncai Zhang,Yonggang Yan
In vitro cytocompatibility of ternary biocomposite of dicalcium phosphate (DCP) and calcium sulfate (CS) containing 40 wt% poly (amino acid) (PAA) was evaluated using L929 fibroblasts and MG-63 osteoblast-like cells. Thereafter, the biocompatibility of biocomposite in vivo was investigated using an implantation in muscle and bone model. In vitro L929 and MG-63 cell culture experiments showed that the composite and PAA polymer were noncytotoxic and allowed cells to adhere and proliferate. The scanning electron microscope (SEM) confirmed that two kinds of cells maintained their phenotype on all of samples surfaces. Moreover, the DCP/CS/PAA composite showed higher cellular viability than that of PAA; meanwhile, the cell proliferation and ALP activity were much higher when DCP/CS had added into PAA. After implanted in muscle of rabbits for 12 weeks, the histological evaluation indicated that the composite exhibited excellent biocompatibility and no inflammatory responses were found. When implanted into bone defects of femoral condyle of rabbits, the composite was combined directly with the host bone tissue without fibrous capsule tissue, which shown good biocompatibility and osteoconductivity. Thus, this novel composite may have potential application in the clinical setting.
研究领域:
骨科工程
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Anlin Yin,Xiaorong Lan,Weihua Zhuang,Zhonglan Tang,Yan Li,Yunbing Wang
In this study, we fabricated native tissue mimicking a bilayered small-diameter vascular graft based on PEGylated chitosan (PEG–CS) and poly(l-lactic acid-co-ε-caprolactone) (PLCL). PEG–CS possesses good hemocompatibility, and as the inner layer of a vascular graft, it is more likely to swell and deform to debris, causing a blood clot, so the PLCL was added to stabilize it. In order to optimize the performance of the inner layer, PEG–CS blends with different ratios of PLCL (P/C:PLCL) casting films were fabricated, and endothelial cell compatibility and hemocompatibility were tested. The outer layer of the graft was fabricated via electrospinning with PLCL blends with different ratios of water-soluble polyethylene glycol. After water treatment, we tested smooth muscle cell penetration. Designing a two-layer vascular graft structure with an inner layer of P/C:PLCL = 1:6 and an outer layer being composed of a PLCL blend with 12% polyethylene glycol offers very good hemocompatibility and fast cell growth and also reserves well mechanical properties with good cell infiltration. Therefore, this kind of graft has a high potential to be used as an artificial blood vessel.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Tian Huang,Yue Ma,Huangjingyi Chen,Siqing Zhang,Liang Liu,Meijuan Chen,Ruoxian Jia,Lin Lin,Muhammad Wajid Ullah,Yimin Fan
Uncontrolled traumatic blood loss is a leading cause of hemorrhagic shock and death, highlighting the critical need for compressible and rapid hemostatic first-aid materials. In this study, silk nanofibers (MA-SNFs) were prepared through maleic acid (MA) hydrolysis decorated with enriched carboxyl groups. The MA-SNFs were then combined with hyaluronic acid (HA) through EDC/NHS crosslinking to form a porous sponge ( i.e. , MA-SNF/HA) through freeze-drying. The fabricated MA-SNF/HA sponges demonstrated excellent blood compatibility (hemolysis ratio < 5 %), outstanding hemocompatibility (blood clotting index (BCI) < 35 % within 60 s), and good cytocompatibility (cell viability >85 %). Among the different sponges prepared, M4-H6 (MA-SNFs: HA = 4:6) exhibited the best liquid reabsorption capacity after 80 % compression, outperforming M6-H4 and M5-H5 sponges. Furthermore, M4-H6 sponge absorbed liquid rapidly (~30 s) while matching the liquid absorption capacity of commercial gelatin sponge (GS), which require over 5 min for similar absorption (2232.84 ± 141.69 %). These findings suggest that M4-H6 sponge is highly suitable for compressible hemostasis applications and provide further insights into its potential hemostatic mechanism.
研究领域:
生物医学工程
·
材料科学
·
创伤急救
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Technological Sciences
·
2020
·Vol. 63
·期 6
·992-1004
·
IF 2.302
Shi Shun,Yu ShuangJiang,Li Gao,He ChaoLiang,Chen XueSi
A lack of biological activity hinders the application of synthetic hydrogels in tissue engineering and regenerative medicine. However, the use of glycopolypeptides in hydrogel synthesis may provide the materials with the desired biological activities. Herein, we prepared three in situ -forming hydrogels from various phenol-functionalized glycopolypeptides. The gelation time, mechanical properties, degradation properties, and biocompatibility of the hydrogels were assessed. Gelation time ranged from 11 to 380 s, depending on the concentration of horseradish peroxidase. The galactose-modified polypeptide hydrogel showed the highest storage modulus with an obvious stress relaxation phenomenon. The prepared hydrogels exhibited good degradation properties and compatibility to cells and tissues. Furthermore, the rate of immune cell accumulation around the mannose-modified polypeptide hydrogel was the fastest among the hydrogels.
研究领域:
生物材料学
·
组织工程
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Tian-Ming Liu,Jing-Jie Xu,Yun-Ren Qiu
In order to ameliorate the biocompatibility of polysulfone (PSf), sulfonated hydroxypropyl chitosan (SHPCS) was grafted from PSf membrane material by Schiff-Base reaction. The original and modified membranes were characterized by attenuated total reflectance-Fourier transform infrared spectroscope (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), water contact angle (WCA) measurement, tensile strength test and antibacterial test in vitro, and the results indicated that the PSf modified by SHPCS (PSf-SHPCS) was synthesized successfully, the hydrophilicity of PSf-SHPCS membrane was improved to a great extent, all the membranes possessed good stability in physiological condition and the PSf-SHPCS membrane had good antibacterial property. Protein adsorption, platelet adhesion, hemolysis assay, plasma recalcification time, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and whole blood clotting time were executed to evaluate the hemocompatibility of membranes decorated by SHPCS, and the results demonstrated that the modified membrane had fine hemocompatibility.
研究领域:
生物材料学
·
医学工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 130
·107949-107956
·
IF 3.84
Xuemin Yu,Zhu Xiong,Jinglong Li,Ziyang Wu,Yunze Wang,Fu Liu
Poly(lactic acid) (PLA) has attracted growing attention as a sustainable and environmentally benign membrane material. The good biocompatibility/hemocompatibility is essential for hemodialysis membranes. To circumvent the inadequate hydrophilicity/biocompatibility/hemocompatibility, we have developed a feasible strategy that enables the persistent PEGylation on PLA membranes via micro-swelling and subsequent UV-initiated crosslinking of poly(ethylene glycol)diacrylate (PEGDA). The content of DMSO and PEGDA in the reaction solution was crucial to control the surface PEGylation kinetics. Besides, the influence of PEGylation on membrane chemistry, morphology, hydrophilicity, water flux and dynamic fouling resistance to BSA was investigated. It was demonstrated that the biocompatibility/hemocompatibility was significantly improved by the surface PEGylation in terms of reduced BSA adsorption, extended APTT and alleviative platelet adhesion.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2023
·
IF 4.7
Cheng Liu,Huimin Gao,Gaoqi Sun,Xuefeng Jiang,Saijie Song,Jun Zhang,Jian Shen
Vascular transplantation is an effective strategy against cardiovascular diseases (CVD), and artificial vascular patches are of urgent need across the world. In this work, we designed a multifunctional decellularized scaffolds (DCS)-based vascular patch for porcine vascular repair. Ammonium phosphate zwitter-ion (APZI) and poly(vinyl alcohol) (PVA) hydrogel were coated on the surface of DCS to improve the mechanical properties and biocompatibility of an artificial vascular patch. Then a heparin (Hep)-loaded metal–organic framework (MOF) further decorated the artificial vascular patches to inhibit blood coagulation and promote vascular endothelialization. The obtained artificial vascular patch showed suitable mechanical properties, good biocompatibility, and blood compatibility. In addition, the proliferation and adhesion of endothelial progenitor cells (EPCs) on the surface of artificial vascular patch improved a lot when compared with unmodified PVA/DCS. According to the results of B-ultrasound and CT images, the artificial vascular patch could maintain the patency of the implant site after implanting into the pig carotid artery. The current results solidly support that a MOF-Hep/APZI-PVA/DCS vascular patch would be an excellent vascular replacement material.
研究领域:
生物医学工程
·
心血管外科
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）