Current Research in Food Science
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2024
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IF 6.2
Weibo Lu,Yunyan Li,Lijun Ge,Honghai Wang,Ting Liu,Qiaoling Zhao,Zhujun Mao,Jingjing Liang,Pingya Wang,Kang Chen,Jing Xue,Qing Shen
The authenticity of fish products has become a widespread issue in markets due to substitution and false labeling. Lipidomics combined with chemometrics enables the fraudulence identification of food through the analysis of a large amount of data. This study utilized ultra-high-performance liquid chromatography (UHPLC)-QE Orbitrap MS technology to comprehensively analyze the lipidomics of commercially available basa catfish and sole fish. In positive and negative ion modes, a total of 779 lipid molecules from 21 lipid subclasses were detected, with phospholipid molecules being the most abundant, followed by glycerides molecules. Significant differences in the lipidome fingerprinting between the two fish species were observed. A total of 165 lipid molecules were screened out as discriminative features to distinguish between basa catfish and sole fish, such as TAG(16:0/16:0/18:1), PC(14:0/22:3), and TAG(16:1/18:1/18:1), etc. This study could provide valuable insights into authenticating aquatic products through comprehensive lipidomics analysis, contributing to quality control and consumer protection in the food industry.
研究领域:
食品科学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 1
·250-258
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IF 3.639
Rui Liu,Hengye Chen,Shuo Wang,Liuna Wei,Yongjie Yu,Wei Lan,Jian Yang,Lanping Guo,Haiyan Fu
BACKGROUND Sesame oil has an excellent flavor and is widely appreciated. It has a higher price than other vegetable oils because of the high price of its raw materials, and different processing techniques also result in products of different quality levels, which can command different prices. In the market, there is a persistent problem of adulteration of sesame oil, driven by economic interests. The screening of volatile markers used to distinguish the authenticity of sesame oil raw materials and production processes is therefore very important. RESULTS In this work, six markers related to the production processes and raw materials of sesame oil were screened by gas chromatography–tandem mass spectrometry (GC-MS/MS) combined with chemometric analysis. They were 3-methyl-2-butanone, 2-ethyl-5-methyl-pyrazine, guaiacol, 2,6-dimethyl-pyrazine, 5-methyl furfural, and ethyl-pyrazine. The concentration of these markers in sesame oil is between 10 and1000 times that found in other vegetable oils. However, only 3-methyl-2-butanone and 2-ethyl-5-methyl-pyrazine differed significantly as the result of the use of different production processes. Except for guaiacol, which was mainly derived from raw materials, the other five compounds mentioned above all result from the Maillard reaction during thermal processing. The six compounds mentioned above are sufficient to distinguish fraud involving sesame oil raw materials and production processes, and can identify accurately adulteration levels of 30% concentration. CONCLUSION In this study, the classification markers can identify the adulteration of sesame oil accurately. These six compounds are therefore important for the authenticity of sesame oil and provide a theoretical basis for the rapid and accurate identification of the authenticity of sesame oil. © 2021 Society of Chemical Industry.
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Conghui Zhou,Yuwei Jia,Qi Zhang,Wenhua Huang,Jizhong Yan,Xuhui Ying,Hui Zhang
Ethnopharmacological relevance Pseudobulbus Cremastrae seu Pleiones (PCSP) is a multi-source traditional Chinese medicine (TCM) with diverse chemical compositions and toxicity levels. The authenticity identification and safety evaluation of PCSP have attracted widespread attention in clinical applications. Aim of this study The objective of this study was to evaluate the authenticity and safety of commercially available PCSP. Materials and methods Morphological and microscopic identification, HPLC chromatogram, UPLC-Q-TOF-MS/MS with molecular networking were applied to the authenticity identification of PCSP. The safety of different PCSPs was evaluated by acute toxicity in zebrafish at maximum non-lethal concentration (MNLC) and 10% lethal concentration (LC 10 ). Intestinal toxicity of PCSP was assessed through histological staining, intestinal goblet cells, neutrophils, and intestinal opacity. Results Four sources of PCSP varied in size, epidermal longitudinal grooves, and microscopic features. GNPS analysis identified 61, 47, 44, and 56 chemical compounds in Cremastra appendiculate (CA), Oreorchis patens (Lindl.) Lindl. (OPL), Iphigenia indica A. Gray (IIG), and Tulipa edulis (Miq.) Baker (TEB). Colchicine and militarine, were discovered as distinguishing markers. Acute toxicity in zebrafish ranked as follows: IIG > OPL > CA > TEB. Further studies on the intestinal toxicity of the authentic PCSP (CA, OPL) showed that CA induced less damage with a smaller lumen area, fewer neutrophils and goblet cells, and reduced peristalsis inhibition compared to OPL, indicating greater safety. Conclusion Four different sources of PCSP were accurately distinguished based on three dimensions: character, components, and toxicity. OPL and CA were considered as genuine products, while CA with lower toxicity was more suitable for clinical applications.
研究领域:
药理学
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毒理学
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中药学
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DOI
PubMed
高影响因子
ANALYST
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2022
·Vol. 147
·期 21
·4857-4865
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IF 5.227
Yiran Wang,Yuze Li,Xiao Wang,Dehua Yu,Zongxiu Nie
Hongmu, a Chinese customary noun representing 29 kinds of wood species such as some Pterocarpus species (abbreviated as spp. hereinafter), Dalbergia spp. and Diospyros spp., is popular among Chinese people due to the furniture made from it. The slow regeneration of hongmu resources led to a decline in production, making hongmu prices high and illegal businesses profit from it. Therefore, it is necessary to identify and distinguish different varieties of hongmu for commercial trade. Herein, a cost-effective and rapid methodology was first developed via atmospheric pressure glow discharge mass spectrometry (APGD-MS) to classify three Dalbergia spp. and three Pterocarpus spp. Meanwhile, principal component analysis (PCA) was further applied to distinguish wood species and six kinds of hongmu extracts were able to be approximately separated into six units. Besides, hongmu could be clearly distinguished from their counterfeits, such as Guibourtia spp., using the method provided here. This method may provide a timely and necessary way for the determination of ingredients and identification of the authenticity of hongmu.
研究领域:
木材科学
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分析化学
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植物分类学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2025
·Vol. 192
·期 3
·1-10
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IF 5.3
Liu Jianghua,Wang Yu,Zhang Xinyue,Huang Mingquan,Li Guoliang
A novel direct lysis method combined with amplification-free CRISPR/Cas12a-SERS genosensor was for the first time developed to rapidly and sensitively identify meat adulteration. Notably, polystyrene (PS) microspheres, with distinct shrinking and swelling properties, were dexterously employed to encapsulate biological-silent Raman reporter 4-mercaptobenzonitrile (4-MBN) and act as a controlled-release signal probe. Target DNA activated the trans -cleavage activity of CRISPR/Cas12a towards ssDNA linked with PS microsphere to liberate the signal probe, which was able to release numerous Raman reporters after treatment with THF solution, resulting in high signal amplification. Through this platform, trace target DNA was deftly transformed into a sensitive Raman signal and could be on-site determined through a portable Raman equipment. Under optimized conditions, this strategy displayed good linearity in the range 1–450 ng/μL ( R 2 = 0.9943) and favorable sensitivity with limit of detection as low as 0.23 ng/μL without any pre-amplification. Moreover, it exhibited good applicability to on-site identification of commercial meat samples in complicated food matrix. In addition, DNA extraction by direct lysis and amplification-free detection realized ultrafast meat adulteration determination within 35 min from sampling to result. This method possessed great potential in rapid and on-site accurate determination of meat authenticity. Graphical abstract
研究领域:
食品科学
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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2025
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IF 2.7
Zhenchao Shao,Shuangshuang Di,Jie Lian,Honggang Nie
Zhongzi purple rice is recognized as a nutritionally superior whole-grain variety, containing higher levels of protein, iron, dietary fiber, and vitamin B6 compared to conventional rice. While the nutritional profile of Zhongzi purple rice is well-established, the spatial distribution and structural specificity of its lipid components, especially germ-specific triacylglycerols (TAGs), remain poorly characterized. This study employs a multimodal mass spectrometric strategy to investigate the lipidomic uniqueness of the Zhongzi purple rice. The strategy combines matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) for spatial analysis, high-resolution mass spectrometry (HRMS) for primary analysis, and high-resolution tandem mass spectrometry with atmospheric pressure chemical ionization (APCI-HRMS/MS) for secondary analysis. MALDI-MSI identified seven metabolites in Zhongzi purple rice that exhibited germ-specific accumulation. In contrast, these metabolites showed markedly reduced signal intensities and lacked structured spatial distribution in conventional purple, black, and brown rice. Using a combination of lipidomic database interrogation, HRMS molecular formula validation, and APCI-HRMS/MS structural elucidation, we identified these metabolites as unsaturated fatty acid-enriched TAGs. Specifically, they were TAG(16:0/16:0/18:2), TAG(16:0/16:0/18:1), TAG(16:0/18:1/18:3), TAG(16:0/18:1/18:2), TAG(16:0/18:1/18:1), TAG(18:1/18:2/18:2), and TAG(18:1/18:1/18:2). The high unsaturation of these TAGs contributes to improved lipid hydrolysis efficiency, metabolic health benefits, and oxidative stability. These properties highlight Zhongzi purple rice as a potential functional food source. Due to their germ-specific accumulation, these compounds can act as potential biomarkers for authenticating Zhongzi purple rice and detecting adulterants. This provides a practical solution to commercial challenges in the industry. This study pushes the boundaries of cereal lipidomics through the integration of spatially resolved imaging and structural validation. The results shed light on the role of lipids in driving the nutritional and metabolic benefits of whole grains.
研究领域:
食品科学
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脂质组学
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分析化学
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营养学
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分子生物学
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代谢组学
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作物品质研究
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产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
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2024
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IF 2.6
Xin Tong,Xijian Wu,Xing Zhang,Limin Zhang,Shaohua Huang,Zhen Wu
In this study, we established a novel chiral metabolomic methodology for determining the authenticity and geographical origin of grape wines. We investigated the metabolites present in Chinese grape wine samples using high-performance liquid chromatography coupled with 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDAA) and o-phthaldialdehyde (OPA), respectively. The data were analyzed using principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA), respectively. We identified 11 amino acids in the OPA mode and 18 amino acids in the FDAA mode, serving as discriminative markers for the wine samples. The chiral FDAA mode demonstrated an enhanced degree of separation among various group samples compared to the nonchiral OPA mode, leading to superior classification outcomes. This study underscores the efficacy of the chiral FDAA mode in achieving optimal separation for different wine types, grape varieties, and geographical regions.
研究领域:
食品科学
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分析化学
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代谢组学
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产品关联
DOI
高影响因子
Foods
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2025
·Vol. 14
·期 19
·3360
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IF 5.1
Yaqi Liu,Zhenli Cai,Yao Fan,Xingcai Wang,Meixia Wu,Haiyan Fu,Yuanbin She
Aimed at addressing the increasingly serious problem of adulteration in Xihu Longjing, a catechin-targeted nano-enhanced visual and fluorescent dual-mode sensor array was constructed by nano porphyrins and quantum dots (QDs) for the precise analysis of Xihu Longjing from adjacent origins. This sensor array realizes the quantitative analysis of catechin enantiomers in Xihu Longjing through the selective combination of sensing units. It can accurately identify adjacent Xihu Longjing teas with different grades and storage times and can precisely detect samples with a low proportion of adulteration. At the same time, the flavor quality and antioxidant performance of Xihu Longjing tea can also be quantitatively evaluated. The dual-mode sensor array design proposed in this study provides a new idea for detecting minor differences in food authenticity and has significant application value for quality control in the tea industry.
研究领域:
食品科学
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分析化学
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纳米生物技术
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茶叶质量控制
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分子识别
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传感技术
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
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2025
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IF 8.2
Chongxin Liu,Simona Grasso,Nigel Patrick Brunton,Qi Yang,Shaobo Li,Li Chen,Dequan Zhang
The origin traceability of lamb is a longstanding concern for consumers. Despite the widespread application of untargeted metabolomics in meat origin traceability, challenges remain in achieving rapid and accurate identification of biomarkers. This study utilized untargeted metabolomics to analyse five breeds of geographical indication lamb, obtaining profile data comprising a total of 4139 metabolites. Using random forest recursive feature elimination, 29 potential biomarkers were initially identified, which showed significant breed-specific and production environment-related variations. Upon further assessment, a refined panel of 14 metabolic biomarkers demonstrated optimal accuracy and robustness in tracing lamb origin. When combined with the Naive Bayes algorithm, these biomarkers yielded the highest classification accuracy among all evaluated machine learning methods. The random forest recursive feature elimination presents a practical approach for handling high-dimensional metabolomics datasets compared to previous analytical methods. These findings also provide valuable insights into the development of machine learning-based biomarker panels, greatly enhancing the breed-specific traceability of lamb.
研究领域:
食品科学
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分析化学
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代谢组学
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机器学习
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畜牧学
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食品真实性鉴定
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Jianmei Li,Qi Sun,Haiyu Li,Zongyao Zhao,Ting Gong,Mengqin Xiang,Feng Gao,Yong Tang
Traditional DNA purification methods face limitations in efficiency and complexity, particularly for food authenticity verification. To address this, we developed novel magnetic nanocomposites by integrating polydopamine-coated Fe 3 O 4 nanoparticles (Fe 3 O 4 @PDA) with zirconium-based metal-organic frameworks (Zr-MOFs) functionalized via varying organic ligands (terephthalic acid, 2-aminoterephthalic acid, and 1,2,4-benzenetricarboxylic acid). The resulting materials—MUIO-66, MUIO-66-NH₂, and MUIO-66-COOH—were characterized for structure, magnetism, and DNA adsorption performance. MUIO-66-COOH exhibited superior efficiency, achieving >80 % DNA recovery from camel meat under optimized conditions (25 °C, 10 min), outperforming commercial kits. Adsorption kinetics and isotherms revealed a multilayer process (Freundlich model) governed by pseudo-second-order kinetics, driven by "salt-bridge" interactions between DNA phosphate groups and surface carboxyl moieties. The method demonstrated high precision (RSD = 2.75 %), reproducibility, and reusability (>81 % efficiency after 5 cycles). This work establishes a rapid, ligand-tunable magnetic solid-phase extraction (MSPE) platform for high-quality DNA extraction, advancing biosensing applications in food authenticity.
研究领域:
纳米技术
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遗传学
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食品科学
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材料科学
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分析化学
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生物技术
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
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IF 3.8
Yan Qiaofang,Huang Fei,Wang Chuyang,Zhan Min,Zhang Qiaoxuan,Huang Di,Yan Jun,Lin Haibiao,Huang Xianzhang,Han Liqiao
Digoxin, a cardiac glycoside, is widely used in the treatment of cardiovascular diseases. Due to its narrow therapeutic range, precise monitoring of its blood concentration is essential. A reference measurement procedure (RMP) is pivotal for ensuring result accuracy and comparability. The RMP for serum digoxin by ID-LC-MS/MS was optimized with sample pre-treatment and detection processes, and the bracketing calibration method was used, which facilitates more accurate measurement, especially for extreme concentrations. The performance of this optimized RMP was thoroughly evaluated. The limit of detection (LoD) was 0.05 ng/mL (0.06 nmol/L) and the lowest limit of quantification (LLoQ) was 0.10 ng/mL (0.13 nmol/L). The intra- and inter-assay imprecisions were 2.24%, 2.51%, 1.40% and 1.72%, 1.65%, 0.97% at 0.5, 2.0, 5.0 ng/mL, respectively. Recoveries were 99.63 to 101.42% and the linear response ranged from 0.1 to 10.0 ng/mL. The relative bias was 0.41% and 2.00% of our results compared with the median of all participating reference laboratories for IFCC-RELA (External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine) 2023A and 2023B. The uncertainty, calibration and measurement capability (CMC) of this method were also evaluated. The optimized RMP was applied in the Trueness Verification Plan of Southern China, which indicates significant differences among clinical systems, highlighting the need for standardization efforts. In addition, two commonly used clinical systems which employed immunoassay methods were compared with this optimized RMP, and 26 individual serum samples were analyzed. The good correlations indicate the feasibility of standardization for serum digoxin. The optimized RMP serves as an accurate reference baseline for routine methods, aiming to enhance the accuracy and precision of measurements in clinical laboratories. Graphical Abstract
研究领域:
临床化学
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药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Rong Zhang,Wei Jia
The efficiently extraction and accurately quantify of β-carotene and its metabolites are crucial for authenticity and traceability in goat milk. Nevertheless, its reliability can be largely improved. In this study, meticulously designed native ESI-MS, fluorescence spectroscopy and molecular docking in combination with cold-induced acetonitrile aqueous two-phase separation system weaken the interaction between β-lactoglobulin and β-carotene metabolites and realized the efficiently extraction. Furthermore, established non-targeted quantitative metabolomics with optimal ion source and variable data-independent acquisition minimized the matrix effects and potential ion suppression. Validated atmospheric pressure chemical ionization-ultra high performance liquid chromatography-Orbitrap method showed that β-carotene as distinctive biomarker in cow milk, and retinol, retinaldehyde, retinoic acid and abscisic acid in goat milk. Collectively, the proposed method is a powerful tool to detect cow adulteration risks in goat milk samples and provides valuable information for availability on authenticity of goat milk.
研究领域:
食品科学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）