Fungal invasion is a main factor leading to the loss of postharvest herbs during storage. To prevent fungal invasion and clarify the favorable conditions for the stability of herbs during the storage period, uniform design and three-dimensional response surfaces were applied to investigate the relationship of the mildew degree of Radix Ophiopogonis induced by prevalent fungal isolates and the storage factors including air relative humidity (X1), temperature (X2), and moisture content (X3), in laboratory studies. Mildew degree was evaluated by ergosterol assay of mold isolates through a high-performance liquid chromatography−diode array detector (HPLC-DAD) technique. As a result, storage conditions, which satisfied the following correlation equations established by the relationships between the storage factors and the biomass of mildew (Y), could effectively prevent fungal spoilage: Y = −0.2484084028 + 0.00030711966572 × X3 × X3 + 0.00018881361186 × X1 × X2 − 0.00029473040679 × X2 × X3 ≤ 0; Y = −0.2697457586 + 0.004127756022 × X1 − 0.000015977780492 × X1 × X1 + 0.00021906984606 × X2 × X3 ≤ 0; Y = −0.325655811 + 0.015464432582 × X2 + 0.00004779394354 × X1 × X1 − 0.00021743815482 × X2 × X2 ≤ 0. This compositive methodology might be useful to predict the occurrence of fungal invasion.
Ruoyu Mu,Yuhan Zhang,Lingli Yan,Zhencheng Liao,Yushun Yang,Huanxing Su,Lei Dong,Chunming Wang
The globally high prevalence of peripheral artery diseases poses a pressing need for biomaterials grafts to rebuild vasculature. When implanted, they should promote endothelial cells (ECs) adhesion both profoundly and selectively—but the latter expectation remains unfulfilled. Here, this work is inspired by fungi that invade blood vessels via the “bridge” of galectins that, secreted by ECs, can simultaneously bind carbohydrates on fungal surface and integrin receptors on ECs. A glucomannan decanoate (GMDE) substrate mimicking fungal carbohydrates that highly and preferentially supports ECs adhesion while rejecting several other cell types is designed. Electrospun GMDE scaffolds efficiently sequester endogenous galectin-1—which bridges ECs to the scaffolds as it functions in fungal invasions—and promote blood perfusion in a murine limb ischemic model. Meanwhile, the application of GMDE requires no exogenous pro-angiogenic agents and causes no organ toxicity or adverse inflammation in mice, highlighting its high safety of potential translation. This glycan material, uniquely mimicking a microbial action and harnessing a secreted protein as a “bridge,” represents an effective, safe, and different strategy for ischemic vascular therapy.
危险品化学品经营许可证（带存储）