Biomed Research International
·
2016
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IF 2.134
Jie Liu,Lvhui Sun,Niya Zhang,Jiacai Zhang,Jiao Guo,Chong Li,Shahid Ali Rajput,Desheng Qi
The current study was to better understand the potential factors affecting aflatoxin B1 (AFB1) accumulation varies between different grains. The nutrient composition and contents of defatted substrates were determined; additionally, according to the nutrient content of the substrates, the effects of starch, soluble sugars, amino acids, and trace elements on AFB1 production and mycelial growth in Czapek-Dox medium were examined. These results verified that removal of lipids from ground substrates significantly reduced the substrate’s potential for AFB1 production by Aspergillus flavus. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly induced AFB1 production up to 1.7- to 26.6-fold. And stachyose more significantly promoted A. flavus growth than the other nutrients. Thus, this study demonstrated that, combined with the nutrients content of grains, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc might play key roles in various grains that are differentially infected by A. flavus. Particularly, two new nutrients (arginine and stachyose) of the grains we found significantly stimulate AFB1 production and A. flavus growth, respectively. The results provide new concepts for antifungal methods to protect food and animal feed from AFB1 contamination.
研究领域:
食品科学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 9
·1698
·
IF 4.926
Tian Gan,Huanli An,Ming Tang,Hui Chen
Leptographium qinlingensisis a bark beetle-vectored pine pathogen in the Chinese white pine beetle (Dendroctonus armandi) epidemic in Northwest China.L. qinlingensiscolonizes pines despite the trees’ massive oleoresin terpenoid defenses. Regulators of G-protein signaling (RGS) proteins modulate heterotrimeric G-protein signaling negatively and play multiple roles in the growth, asexual development, and pathogenicity of fungi. In this study, we have identified threeL. qinlingensis RGSgenes, and the phylogenetic analysis shows the highest homology with the regulators of G-protein signaling proteins sequence fromOphiostoma piceaeandGrosmannia clavigera. The expression profiles of three RGSs in the mycelium ofL. qinlingensistreated with six different terpenoids were detected, as well as their growth rates. Under six terpenoid treatments, the growth and reproduction inL. qinlingensiswere significantly inhibited, and the growth inflection day was delayed from 8 days to 12–13 days. By analyzing the expression level of threeRGSgenes ofL. qinlingensiswith different treatments, results indicate thatLqFlbAplays a crucial role in controlling fungal growth, and bothLqRax1andLqRgsAare involved in overcoming the host chemical resistances and successful colonization.Keywords:Leptographiumqinlingensis;regulators of G-protein signaling;terpene tolerance;host resistances;expression
研究领域:
真菌学
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植物病理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2023
·
IF 4.6
Weiwei Zhang,Liangjun Yang,Hanghang Li,Ping Song,Wanzhen Li,Lin Gui,Kai Yang,Longbao Zhu,Yugui Tao,Fei Ge
2D Molybdenum disulfide (MoS 2 ) nanoplatform were prepared by the liquid ultrasonic stripping method using chitosan (CS) and eugenol (Eg), and a drug loading system based on MoS 2 photothermal effect was constructed (EMC) and applied in antifungal assays. The system not only had good physiological stability but also had an excellent photothermal effect and could effectively inhibit the growth of fungi. The characterization results showed that the average thickness of MoS 2 nanoplatform was 10 nm. In vivo and in vitro experiments showed that the synthesized EMC had excellent photothermal properties, displayed efficient antifungal activity against spoilage fungi such as Saccharomyces uvarum and Penicillium , thus prolonging the shelf life of the fruit. The analysis of chemical quality indexes showed that the good film-forming ability of EMC. This study reported a good photothermal effect of MoS 2 to inhibit fungal growth to protect postharvest fruits.
研究领域:
食品科学
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Advanced Research
·
2021
·
IF 10.479
Xueyang Jin,Ming Zhang,Jinghui Lu,Ximeng Duan,Jinyao Chen,Yue Liu,Wenqiang Chang,Hongxiang Lou
Introduction The increasing morbidity of fungal infections and the prevalence of drug resistance highlighted the discovery of novel antifungal agents and investigation of their modes of action. Iron chelators have been used to treat superficial fungal infections or potentiate the efficacy of certain antifungal drugs. Hinokitiol exhibits potent antifungal activity and iron-chelating ability. However, their relationships have not been established. Objectives This study aims to explore the selectivity of hinokitiol against fungal cells and mammalian cells and determine the role of iron-chelating for the antifungal activity of hinokitiol. Methods Iron probe FeRhonox-1 was used to determine intracellular Fe 2+ content. 5-Cyano-2,3-ditolyl tetrazolium chloride probe and Cell Counting Kit-8 were used to detect the mitochondrial respiratory activities. Quantitative real-time PCR and rescue experiments were performed to determine the effect of iron on the antifungal activity of hinokitiol. The effects of hinokitiol on fungal mitochondria were further evaluated using reactive oxygen species probes and several commercial Assay Kits. The ability of hinokitiol to induce resistance in Candida species was carried out using a serial passage method. The in vivo therapeutic effect of hinokitiol was evaluated using Galleria mellonella as an infectious model. Results Hinokitiol was effective against a panel of Candida strains with multiple azole-resistant mechanisms and persistently inhibited Candida albicans growth. Mechanism investigations revealed that hinokitiol chelated fungal intracellular iron and inhibited the respiration of fungal cells but had minor effects on mammalian cells. Hinokitiol further inhibited the activities of mitochondrial respiratory chain complexes I and II and reduced mitochondrial membrane potential, thereby decreasing intracellular ATP synthesis and increasing detrimental intracellular reductive stress. Moreover, hinokitiol exhibited low potential for inducing resistance in several Candida species and greatly improved the survival of Candida -infected Galleria mellonella . Conclusions These findings suggested the potential application of hinokitiol as an iron chelator to treat fungal infections.
研究领域:
微生物学
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药理学
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真菌学
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医学真菌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2021
·
IF 5.293
Yinrui Guo,Hongya Guo,Lingyan Qiu,Yuanfei Fu,Xiangxiang Zhu,Haiting Zhang,Jian Wang,Diling Chen
It is known that the microbiome affects human physiology, emotion, disease, growth, and development. Most humans exhibit reduced appetites under high temperature and high humidity (HTHH) conditions, and HTHH environments favor fungal growth. Therefore, we hypothesized that the colonic mycobiota may affect the host’s appetite under HTHH conditions. Changes in humidity are also associated with autoimmune diseases. In the current study mice were fed in an HTHH environment (32°C ± 2°C, relative humidity 95%) maintained via an artificial climate box for 8 hours per day for 21 days. Food intake, the colonic fungal microbiome, the feces metabolome, and appetite regulators were monitored. Components of the interleukin 17 pathway were also examined. In the experimental groups food intake and body weight were reduced, and the colonic mycobiota and fecal metabolome were substantially altered compared to control groups maintained at 25°C ± 2°C and relative humidity 65%. The appetite-related proteins LEPT and POMC were upregulated in the hypothalamus ( p < 0.05), and NYP gene expression was downregulated ( p < 0.05). The expression levels of PYY and O-linked β-N-acetylglucosamine were altered in colonic tissues ( p < 0.05), and interleukin 17 expression was upregulated in the colon. There was a strong correlation between colonic fungus and sugar metabolism. In fimo some metabolites of cholesterol, tromethamine, and cadaverine were significantly increased. There was significant elevation of the characteristic fungi Solicoccozyma aeria , and associated appetite suppression and interleukin 17 receptor signaling activation in some susceptible hosts, and disturbance of gut bacteria and fungi. The results indicate that the gut mycobiota plays an important role in the hypothalamus endocrine system with respect to appetite regulation via the gut-brain axis, and also plays an indispensable role in the stability of the gut microbiome and immunity. The mechanisms involved in these associations require extensive further studies.
研究领域:
微生物学
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免疫学
·
神经科学
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分子生物学
·
生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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