The purpose of this work is to develop a plant-derived compounds (PDCs)/chitosan (CS)/gelatin (GL) packaging lining film with high inhibitory activity against the contamination of Zygosaccharomyces rouxii in concentrated apple juice. The PDCs with high anti - Z. rouxii activity were selected from 19 PDCs for pairwise combining, and the combination with synergistic anti - Z. rouxii effect was selected to prepare nanoemulsion. Then, the effect of PDCs emulsion on mechanical and anti - Z. rouxii properties of CS/GL film was investigated. Finally, the prepared PDCs/CS/GL film was used as packaging lining to explore the preservation effect on concentrated apple juice. The results showed that the combination of trans -cinnamaldehyde and geraniol (4:6, v/v) presented the highest synergistic anti - Z. rouxii effect (the value of fractional inhibitory concentration index equaling 0.25). Addition of trans -cinnamaldehyde-geraniol nanoemulsion reduced mechanical properties of CS/GL film, but improved anti - Z. rouxii activity. The CS/GL film containing 1.5 % (v/v) trans -cinnamaldehyde-geraniol nanoemulsion showed high anti - Z. rouxii activity and did not affect the quality of concentrated apple juice, which could be used as a packaging lining for preservation of concentrated apple juice. The PDCs/CS/GL packaging lining film prepared in this work not only presented an enduring and effective inhibitory effect on Z. rouxii contamination in concentrated apple juice, but also avoided the influences of trans -cinnamaldehyde and geraniol on the quality of concentrated apple juice, which provided a new idea for the development of food preservation packaging film.
The critical shortage of antifungal drugs and increasingly emerged resistant fungi urgently call for the development of novel fungicidal agents with translational potential. Although nanodrugs compose a new antifungal arsenal, the fungicidal efficacy barely exceeds clinical molecules. In this study, a CuFeSe 2 -PVP nano-blade, is unexpectedly discovered with ultrapotent intrinsic fungicidal efficacy. This nanostructure is facilely fabricated by a specific aqueous precipitation method with the nucleation of tetragonal eskebornite CuFeSe 2 at PVP chains. The unique semiconductor CuFeSe 2 -PVP demonstrates superior antifungal activity than the commonly used small-molecule drugs and metal nanoparticles in both planktonic fungi and biofilm. Intriguingly, electron microscopy visualizes that the nano-blades cut fungal membranes with fungi surfaces inserted by the blades. Such a membrane-piercing effect results in the loss of important intracellular components, including nucleic acids , proteins, and potassium ions. Increased intracellular reactive oxygen species (ROS) is also observed. Encouragingly, in mice with subcutaneous fungal infection, the nanodrug achieves absolute fungi clearance, effectively alleviating inflammation and promoting collagen fiber recovery. Moreover, the excellent biocompatibility of CuFeSe 2 -PVP promises great potential for further translation. This study not only provides a novel fungicidal drug candidate, but also inspires the future design of anti-fungal nanomedicine .
危险品化学品经营许可证（带存储）