Infection and Drug Resistance
·
2025
·
IF 2.9
Kaixuan Yuan,Benshun Tian,Annan Zhan,Zhuoxi Chen,Long Ye,Yong Ling,Suiling Liu,Xuejiao Bai,Yunhu Zhao
Purpose Inactivating filamentous fungi (molds) reduce toxin production and transmission. This study evaluated the efficacy of formic acid (FA) fumigation to identify optimal conditions and its effects on fungal morphology. We aim to develop an efficient inactivation protocol for molds and provide valuable insights into laboratory techniques for fungal inactivation.Methods Initially, 238 clinical isolates from 23 species of molds were evaluated for the inactivation efficacy of formic acid, formaldehyde, and peracetic acid. After identifying the optimal sterilizing agent, 188 isolates from 16 clinically significant species were tested. Four concentrations (30%, 50%, 70%, and 100%) and six-time intervals (4 to 14 hours) were tested to optimize fumigation conditions. Morphological changes were analyzed using Aspergillus fumigatus ATCC MYA-3626 as the standard strain.Results The inactivation rates of 238 molds fumigated with formic acid, formaldehyde, and peracetic acid for 24 hours were 99.16%, 89.08%, and 90.35% (P < 0.05), indicating that formic acid was more effective than formaldehyde and peracetic acid. Subsequently, 188 isolates from 16 species were fumigated with formic acid at concentrations of 30%, 50%, 70%, and 100% for 24 hours, resulting in inactivation rate of 73.9%, 98.4%, 100%, and 100% (P < 0.05). Inactivation rate increased significantly with longer exposure times: 51.1% at 4 hours, 80.9% at 6 hours, 89.4% at 8 hours, 94.7% at 10 hours, 100% at 12 hours, and 100% after 14 hours (P < 0.05). After fumigation, A. fumigatus ATCC MYA-3626 growth ceased. Electron microscopy revealed irregular folds and roughness on spore surfaces, as well as collapsed or dead spores.Conclusion Formic acid demonstrated superior inactivation efficacy compared to formaldehyde and peracetic acid. At a concentration of 70% and a fumigation duration of 12 hours, the inactivation of molds was most effective.
研究领域:
真菌学
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微生物学
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临床实验室技术
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真菌病理学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 5
·1103
·
IF 4.2
Yuwei Xing,Cong Liu,Xumeng Cui,Haonan Lv,Jun Wang
Nymphaea candida Presl is a rare hardy water lily at risk of extinction and has been included on the ‘Red List’ of threatened species of the International Union for the Conservation of Nature. To protect germplasm resources and propagate N. candida seedlings, this study conducted tissue culture and found that pathogenic endophytic fungal infection was the main reason for failure of tissue culture. Compared with the stems and leaves, the roots of N. candida had the highest rates of fungal infection during tissue culture. Subsequently, three isolated endophytic fungi, Fusarium oxysporum , Phytopythium helicoides , and Alternaria sp., showed the highest frequency of occurrence in tissue culture. Furthermore, an antifungal formulation comprising 0.1 μg/mL pyrimidin suspension, 1 μg/mL mancozeb wettable powder, and 1 μg/mL carbendazim was constructed and could reduce the infection rates of root and stem tissues to 7.5% and 0%, respectively. Finally, the usefulness of this antifungal formulation for inhibition of endophytic fungi in tissue culture of N. candida was validated. This study not only provides important technical support for mass production of seedlings of N. candida , but also provides a scientific reference for the protection of endangered aquatic plant species.
研究领域:
植物学
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保护生物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Xiaoxue Ge,Meng Gao,Bairong He,Nannan Cao,Kerun Li,Yong Liu,Sanmei Tang,Kai Liu,Jing Zhang,Fang Hu,Lei Zheng,Bo Situ
The increasing resistance among fungi to antimicrobials are posing global threats to health. Early treatment with appropriate antifungal drugs guided by the antifungal susceptibility testing (AFST) can dramatically reduce the mortality of severe fungal infections. However, the long test time (24–48 h) of the standard AFSTs cannot provide timely results due to the slow growth of the pathogen. Herein, we report a new AFST that is independent of growth rate analysis using a luminogen with aggregation-induced emission characteristics (AIEgen) named DMASP. DMASP is a water-soluble small-molecule probe that can readily penetrate the dense fungal cell wall . Based on its mitochondria-targeting ability and AIE characteristics, fungal activity can be dynamically indicated via real-time fluorescence monitoring. This allows fungal susceptibility to various antimicrobials to be assessed within 12 h in a wash-free, one-step manner. This method may serve as a promising tool to rapidly detect possible drug-resistant fungal strain and guide the precise use of antimicrobial against fungal diseases.
研究领域:
微生物学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2025
·Vol. 11
·期 3
·209
·
IF 4
Jiao Yao,Sai Huang,Lingfeng He,Shengyun Wei,Wei Yang,Qiangxin Zhang,Weihua Wang,Xiaoqin Yang,Sida Xie,Yunxian Li,Ping Zhao,Guolei Zhu
One novel C10polyacetylene rhamnoside, 4,6,8-decatriyne-1-O-α-L-rhamnopyranoside, named xylariside A (1), together with two novel C10polyacetylene quinovopyranosides, 4,6,8-decatriyne-1-O-α-D-quinovopyranoside, xylariside B (2), and 8E-decaene-4,6-diyne-1-O-α-D-quinovopyranoside, xylariside C (3), were obtained from the solid fermentation ofXylariasp. VDL4, an endophytic fungus isolated fromVaccinium dunalianumwight (Ericaceae). Their chemical structures were elucidated through a combination of spectroscopic techniques. The antifungal activities of these compounds were evaluated in vitro against four phytopathogenic fungi (Fusarium oxysporum,Botrytis cinerea,Phytophthora capsici, andFusarium solani). Compound2demonstrated significant antifungal activities, with minimum inhibitory concentration (MIC) values ranging from 3.91 to 7.81 μg/mL. Compound2’s effectiveness levels were similar to those of the reference drugs thiabendazole and carbendazim (each MIC = 0.98−15.62 μg/mL). Xylariside B (2) was further evaluated againstB. cinereain vivo. It exhibited remarkable efficacy in both the prevention and treatment of tomato and strawberry gray mold. Molecular docking studies confirmed the antifungal mechanism of compound2by revealing its binding interactions with key enzyme targets inB. cinerea, thereby supporting the observed in vitro and in vivo results. Additionally, compound2showed effective inhibition of α-glucosidase, with IC50values of 5.27 ± 0.0125 μg/mL.
研究领域:
植物化学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Zhibo Hu,Yiwen Tao,Xingyu Tao,Qinhua Su,Jiachun Cai,Can Qin,Weijia Ding,Chunyuan Li
A new monosesquiterpene diacetylgliocladic acid (1), a new dimeric sesquiterpene divirensol H (9), and two exceptionally novel trimeric sesquiterpene trivirensols A and B (11 and 12), together with another eight known congeners, were purified from an endophytic fungus Trichoderma virens FY06, derived from Litchi chinensis Sonn. whose fruit is a delicious and popular food. All of them were identified by comprehensive spectroscopic analysis, combined with biosynthetic considerations. Trivirensols A and B are unprecedented trimers of which three subunits are connected by two ester bonds of the sesquiterpene class. Relative to the positive control triadimefon, all the tested metabolites showed strong inhibitory activities against at least one phytopathogenic fungus among Penicillium italicum, Fusarium oxysporum, Fusarium graminearum, Colletotrichum musae, and Colletotrictum gloeosporioides. Notably, as metabolites of the endophytic fungus from L. chinensis, they all presented strong antifungal activities against C. gloeosporioides which causes anthracnose in L. chinensis.
研究领域:
植物化学
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微生物学
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药物化学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2016
·
IF 2.134
Yan Li,Song Liu,Yukun Qin,Ronge Xing,Xiaolin Chen,Kecheng Li,Pengcheng Li
In this study, three pyrimethanil grafted chitosan (PML-g-CS) derivatives were obtained. The structures of the conjugates were confirmed by FT-IR, 1H NMR, and EA. The grafting ratios were measured by HPLC. Antifungal properties of pyrimethanil grafted chitosan (PML-g-CS) derivatives against the plant pathogenic fungi Rhizoctonia solani and Gibberella zeae were investigated at concentrations of 100, 200, and 400 mg/L. The PML-g-CS derivatives showed enhanced antifungal activity in comparison with chitosan. The PML-g-CS-1 showed the best antifungal activity against R. solani, whose antifungal index was 58.32%. The PML-g-CS-2 showed the best antifungal activity against G. zeae, whose antifungal index was 53.48%. The conjugation of chitosan and pyrimethanil showed synergistic effect. The PML-g-CS derivatives we developed showed potential for further study and application in crop protection.
研究领域:
农业科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2016
·
IF 2.134
Jie Liu,Lvhui Sun,Niya Zhang,Jiacai Zhang,Jiao Guo,Chong Li,Shahid Ali Rajput,Desheng Qi
The current study was to better understand the potential factors affecting aflatoxin B1 (AFB1) accumulation varies between different grains. The nutrient composition and contents of defatted substrates were determined; additionally, according to the nutrient content of the substrates, the effects of starch, soluble sugars, amino acids, and trace elements on AFB1 production and mycelial growth in Czapek-Dox medium were examined. These results verified that removal of lipids from ground substrates significantly reduced the substrate’s potential for AFB1 production by Aspergillus flavus. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly induced AFB1 production up to 1.7- to 26.6-fold. And stachyose more significantly promoted A. flavus growth than the other nutrients. Thus, this study demonstrated that, combined with the nutrients content of grains, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc might play key roles in various grains that are differentially infected by A. flavus. Particularly, two new nutrients (arginine and stachyose) of the grains we found significantly stimulate AFB1 production and A. flavus growth, respectively. The results provide new concepts for antifungal methods to protect food and animal feed from AFB1 contamination.
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Yiyang Li,Yannan Qin,Yi Liu,Bo Yuan,Zhirong Zhao,Jingyuan Wang,Yanhong Ji,Shuai Mao,Feng Ye,Chengcheng Liu,Chuanbin Mao
Onychomycosis is a prevalent nail infection caused by fungi, presenting significant treatment challenges due to poor drug penetration and high recurrence rates. Current treatments, including oral and topical antifungals, often have limited efficacy and can lead to resistance. Transfersomes are lipid vesicles that can penetrate skin for transdermal drug delivery. Here, a t ransfersome (termed THT) capable of penetrating nails to deliver a photosensitizer ( h ematoporphyrin monomethyl ether, HMME) and an antifungal chemotherapeutic drug ( t avaborole) has been developed, achieving a chemo-photodynamic synergistic treatment of onychomycosis. The optimized THT was prepared using a combination of soy lecithin, sodium deoxycholate, and cholesterol in an 8: 4: 0.5 ratio, with a particle size of 200 nm and a 30 % alcohol-water solvent system. This formulation significantly enhanced nail penetration, with the concentration of HMME reaching 330 times higher than that in phosphate-buffered saline. Moreover, THT showed potent fungicidal activity, reducing the survival of Trichophyton rubrum microconidia to as low as 3.24 % under light irradiation, and achieving approximately 90 % treatment efficacy in the ex vivo model. In addition to in vitro and ex vivo verification, an innovative rat onychomycosis model was established to confirm its superior therapeutic efficacy in vivo , with cure rates around 90 % and a significant reduction in fungal hyphae and spores. It is accumulated around and deformed to enter the physiological pores of the nail plate, effectively penetrating to deeper layers at the nail bed. THT, with its high nail penetration efficiency and potent synergistic antifungal activity, represents a promising topical treatment for onychomycosis.
研究领域:
药学
·
微生物学
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生物医学工程
·
皮肤病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 42
·期 6
·e12658
·
IF 1.552
Yuhong Liu,Zhimin Sun,Lili Xiu,Jianying Huang,Fengyan Zhou
Two fungal isolates, F12-20 and F3-1, were collected from postharvest blueberry in China. By phylogenetic analysis with the nuclear ribosomal internal transcribed spacer region and morphological analyses, they were initially identified as Penicillium and Arthrinium sacchari ( A. sacchari ). The results showed that water-soluble chitosan (WCS) and sulfonated chitosan (SCS) could significantly reduce the radial growth of fungal mycelia. The best concentrations were 0.25 and 16 g L –1 for WCS and 0.50 and 64 g L –1 for SCS against Penicillium sp. and A. sacchari , respectively. Importantly, SCS exhibited selective antifungal activity against Botrytis cinerea and A. sacchari . As different species of fungi may respond differently to fungicides, the findings of this study will provide a basis for the development of target-specific pathogen control and will be useful in designing and implementing fungicide resistance management for control postharvest decay, thus extending the storage life of fresh blueberry. Practical application Experimental results obtained herein are useful for designing and implementing fungicide resistance management for control postharvest decay in blueberry to improve marketable fruit and extend the storage life of fresh blueberries. And sulfonated chitosan can be applied to the target-specific pathogen control.
研究领域:
食品科学
·
植物病理学
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DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Zhuang Li,Yahui Huang,Jie Tu,Wanzhen Yang,Na Liu,Wei Wang,Chunquan Sheng
Over the past several decades, invasive fungal infections, especially candidiasis, have caused dramatic morbidity and mortality due to ineffective antifungal drugs and severe drug resistance. Herein, new BRD4–histone deacetylase (HDAC) inhibitors were designed to restore the susceptibility of Candida albicans (C. albicans) to fluconazole (FLC). Interestingly, several compounds showed excellent selectivity against fungal HDACs. In particular, compound B2 showed excellent synergistic effect with FLC against resistant C. albicans (FICI = 0.063) with high selectivity against fungal HDACs (SI = 1653) and low cytotoxicity. Compound B2 effectively synergized with FLC and prevented biofilm formation and morphological transition in resistant C. albicans, potentiating the antifungal activity of FLC in vivo and significantly reducing kidney fungal loads. Thus, this drug combination is promising in the treatment of resistant C. albicans infections.
研究领域:
微生物学
·
药理学
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Zhibo Hu,Zhihui Wu,Qinhua Su,Mingzhe Li,Shanshan Wu,Rongqi Meng,Weijia Ding,Chunyuan Li
To check whether the endophytic fungus from the host plant could produce novel bioactive metabolites induced by the host-derived chemical components, a strategy called “Induction of Endophyte Metabolism by Adding Host Components” (IEMAHC) was put up and applied. Cultivation of the endophytic fungus Botryosphaeria ramosa L29 derived from Myoporum bontioides after adding (2 R , 3 R )-3, 5, 7-trihydroxyflavanone 3-acetate, a constituent of the same plant, into the culture medium, led to the separation of three new chromones , 5-hydroxy-2, 3-dihydroxymethyl-7-methoxychromone ( 1 ), 5-hydroxy-3-acetoxymethyl-2-methyl-7- methoxychromone ( 2 ), 5, 7-dihydroxy-3-hydroxymethyl-2-methylchromone ( 3 ), one new isocoumarin , 8-hydroxy-3-hydroxymethyl-6-methoxy-7-methylisocoumarin ( 4 ), two new δ -lactones, botryopyrone ( 8 ) and (5 S , 8 R )-simplicilopyrone ( 9 ), one new naturally rare 2 (7 H )-oxepinone, botroxepinone ( 10) , and three known compounds ( 5 – 7 ). They were identified through comprehensive analysis of spectroscopic data. HPLC comparative analysis indicated that compounds 8 – 10 were induced products after adding (2 R , 3 R )-3, 5, 7-trihydroxyflavanone 3-acetate as an inducer. Compound 3 exhibited more potent inhibitory activities on Fusarium oxysporum , Fusarium graminearum , Penicillium italicum , and Colletotrichum musae than triadimefon. Compounds 1 , 2 , 4 – 7 and 9 displayed potent antifungal activities towards two or three tested fungi with MIC values equal or superior to triadimefon. The induced products 9 and 10 both showing strong inhibitory activity against C. musae , might play a role in strengthening the host defense against the common mangrove invasive pathogenic fungal genera Colletotrichum .
研究领域:
微生物学
·
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
·
2016
·Vol. 35
·期 5
·1079-1085
·
IF 0.547
Li Yan,Qin Yukun,Liu Song,Li Pengcheng,Xing Rong’e
In this study, three hymexazol-linked chitosan derivatives (HML-CS) were synthesized and their structures confirmed by Fourier transform infrared and elemental analysis. Linkage ratios were measured by high performance liquid chromatography. The derivatives’ antifungal activity against the plant pathogenic fungi Rhizoctonia solani CGMCC 3.28 and Gibberella zeae CGMCC 3.42 were investigated at concentrations of 100, 200, and 400 mg/L. These HML-CS derivatives exhibited stronger antifungal activity than CS alone. HML-CS-1 showed the best antifungal activity against G. zeae , whose antifungal index was 65.9% at 400 mg/L, and also showed the best antifungal activity against R. solani , whose antifungal index was 52.7% at 400 mg/L. This conjugation of CS and HML suggested the presence of synergistic effects between the moieties and indicated that these derivatives possessed great potential as novel fungicides and require further research for the development of applications in crop protection.
研究领域:
农业科学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）