We develop a novel noninvasive wearable biosensor to simultaneously assay multiple key biomarkers in human tears. The sensor is uniquely designed as a convenient-to-use eye patch that can be easily and comfortably fit under eyes. Different sensing regions on the eye patch are modified with specific chromogenic reagents to selectively determine the hydrogen ion (pH), protein, ascorbic acid, and glucose in tears. The assay takes only one drop of tear (∼20 μL) and a 30 s response time. The resulting color signals from each sensing region on the eye patch are captured and analyzed using a smartphone, and a cloud model based on the internet of things concept is recommended, which can extend the application of the eye patch biosensors in point-of-care testing and health monitoring, especially during the pandemic. Tear detection of the human subjects is performed using the proposed method, and the results display good correlation to parallel measurements using assay kits. The results show the great potential and versatility of the proposed eye patch biosensor for noninvasive determination of diverse tear biomarkers, facilitating the widespread application of the tear-based noninvasive wearable biosensor in emerging personal health care and clinical monitoring fields.
Yaling Wu,Wenting Liu,Yujun Zheng,Shuyan Ruan,Yao Chen,Qingqiang Lin,Ruiyun You
A novel nanocomposite hydrogel-based Surface-enhanced Raman scattering (SERS) platform was successfully fabricated using Ag NPs/S-CNF/PVA hydrogel as the core matrix, demonstrating exceptional swelling-deswelling behavior. By evenly distributing a large number of Ag NPs on the surface of S-CNF, a one-dimensional tightly arranged “hot spot” was successfully constructed, which significantly improved the SERS effect and improved the sensitivity and uniformity of the SERS platform. The experimental results show that Rhodamine 6G (R6G) of 10 −7 M can be detected, RSD = 1.90 %. At the same time, PVA hydrogel, as an excellent packaging material, can effectively isolate Ag NPs/S-CNF/PVA composite from the external environment, which helps to maintain the SERS property of the film, but also makes the film more durable and reliable in practical applications. By using different signal molecule modification, different substances were determined. It is worth mentioning that the substrate showed excellent SERS activity on glucose and pH, which has the potential to be applied in non-invasive tear glucose and pH monitoring system. Given its excellent flexibility and repeatability, the substrate is expected to be integrated into a variety of wearable biosensors, thus advancing the development of biological process research. Overall, by combining a flexible SERS 3D substrate, this approach shows great potential and application prospects in the study of biological processes.
Shengnan Yin,Xiaofeng Chen,Runze Li,Linlin Sun,Chanyu Yao,Zheng Li
The abuse or misuse of antibiotics in clinical and agricultural settings severely endangers human health and ecosystems, which has raised profound concerns for public health worldwide. Trace detection and reliable discrimination of commonly used fluoroquinolone (FQ) antibiotics and their analogues have consequently become urgent to guide the rational use of antibiotic medicines and deliver efficient treatments for associated diseases. Herein, we report a wearable eye patch integrated with a quadruplex nanosensor chip for noninvasive detection and discrimination of primary FQ antibiotics in tears during routine eyedrop treatment. A set of dual-mode fluorescent nanoprobes of red- or green-emitting CdTe quantum dots integrated with lanthanide ions and a sensitizer, adenosine monophosphate, were constructed to provide an enhanced fluorescence up to 45-fold and nanomolar sensitivity toward major FQs owing to the aggregation-regulated antenna effect. The aggregation-driven, CdTe–Ln(III)-based microfluidic sensor chip is highly specific to FQ antibiotics against other non-FQ counterparts or biomolecular interfering species and is able to accurately discriminate nine types of FQ or non-FQ eyedrop suspensions using linear discriminant analysis. The prototyped wearable sensing detector has proven to be biocompatible and nontoxic to human tissues, which integrates the entire optical imaging modules into a miniaturized, smartphone-based platform for field use and reduces the overall assay time to ∼5 min. The practicability of the wearable eye patch was demonstrated through accurate quantification of antibiotics in a bactericidal event and the continuous profiling of FQ residues in tears after using a typical prescription antibiotic eyedrop. This technology provides a useful supplement to the toolbox for on-site and real-time examination and regulation of inappropriate daily drug use that might potentially lead to long-term antibiotic abuse and has great implications in advancing personal healthcare techniques for the regulation of daily medication therapy.
危险品化学品经营许可证（带存储）