STEROIDS
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2020
·
IF 1.948
Bairen Du,Jingjing Zhang,Yanjie Dong,Junwei Wang,Longwen Lei,Rengfei Shi
A novel method for determining the testosterone/epitestosterone concentration ratio in human urine was established by capillary electrophoresis with diode-array detector. The urine samples were firstly purified by the solid extraction. The optimal experimental conditions were: running buffer pH = 4.74, 15.0 mmol L −1 HAc-NaAc, separation voltage 25 kV, temperature 25 °C, sample injection pressure 3.43 × 10 3 Pa, and duration 10 s. The testosterone and epitestosterone linear range were determined as 8.0–960.0 ng mL −1 , respectively. The testosterone and epitestosterone detection limits were determined as 4.6 and 4.5 ng mL −1 , respectively. The relative standard deviation was less than 0.36%.
研究领域:
分析化学
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医学检验
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运动科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2020
·Vol. 58
·期 9
·880-886
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IF 1.28
Li Xiao,Yuan Tao,Zhao Ting,Wu Xiaomei,Yang Yaling
An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5–100 μg mL−1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL−1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Marine Drugs
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2021
·Vol. 19
·期 12
·652
·
IF 5.118
Liping Wang,Yiwen Hou,Rong Wang,Qi Pan,Debao Li,Han Yan,Zuyue Sun
This study investigates the inhibitory effect of astaxanthin (AST) on testosterone-induced benign prostatic hyperplasia (BPH) in rats. Except for the sham operation, BPH model rats were randomly assigned to five groups: the BPH model control rats, AST-treated BPH model rats (20 mg/kg, 40 mg/kg, and 80 mg/kg), and epristeride (EPR)-treated BPH model rats. After treatment, as compared with the BPH model control rats, the prostate and ventral prostate weights of the AST-treated rats decreased, while there was a marked decline in the 80 mg/kg AST-treated rats. The same effect was also observed in the prostate index and ventral prostate index. The proliferation characteristics of epithelia observed in the BPH model control group were gradually alleviated in the AST-treated rats. As compared with the BPH model control rats, lower epithelial thicknesses of prostates and fewer secretory granules in epithelia were observed in the AST-treated rats. The superoxide dismutase (SOD) activity of prostates increased in all the AST-treated rats with a significant increase in the 40 mg/kg and 80 mg/kg AST-treated rats. The testosterone (T) and dihydrotestosterone (DHT) levels of prostates in the AST-treated groups were lower than those in the BPH model control group, and a significant decline was found in the T level of prostates in the 40 g/kg and 80 mg/kg AST-treated rats and the DHT level of prostates in the 40 mg/kg AST-treated rats. These results indicate that AST might have an inhibitory effect on T-induced BPH in rats, possibly due to SOD activity regulation and T and DHT levels.Keywords:astaxanthin;benign prostatic hyperplasia;pharmacodynamics;SOD activity
研究领域:
药理学
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泌尿外科
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内分泌学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yabing Shan,Jiayi Li,Ying Chen,Dongmei Li,Yanbo Luo,Yongqiang Pang,Di Chen
The illegal addition of androgenic steroids, such as testosterone and methyltestosterone, to herbal oral liquids poses a significant threat to consumer health. This study presents a novel method for the extraction and determination of these steroids in herbal products using pipette tip solid-phase microextraction combined with liquid chromatography-tandem mass spectrometry. The pipette tips are coated with hydroxyl-functionalized multi-walled carbon nanotubes, ensuring efficient analyte extraction while reducing sample handling and contamination risks. The method was optimized for parameters such as solvent volume, extraction time, and pH, and demonstrated excellent analytical performance. It demonstrated linearity over the range of 5–5000 ng/mL (R 2 > 0.99), with detection limits of 0.96 ng/mL for testosterone and 1.44 ng/mL for methyltestosterone, and limits of quantification of 3.20 ng/mL for testosterone and 4.80 ng/mL for methyltestosterone. Recovery experiments across multiple matrices and concentration levels showed recoveries ranging from 93.8 % to 108.1 %, with relative standard deviations between 1.1 % and 4.5 %. This approach offers a cost-effective, high-throughput solution for the routine detection of illegal steroid additives in herbal and functional food products, providing a valuable tool for regulatory monitoring and consumer safety.
研究领域:
分析化学
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食品安全
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药物分析
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DOI
高影响因子
Scientific Reports
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2023
·Vol. 13
·期 1
·1-12
·
IF 4.6
Li Tao,Tian Yuan,Zhong Quliang,Chen Peng,Zhang Junhao,Du Guangshi,Li Lei,Jiang Yiting,Jiang Kehua
Penile size is closely concerned and short penis contributes serious sexual dysfunction and tremendous psychological problems to couples. Androgen is essential for penile development and testosterone replacement is recommended to patients with micropenis. We previously proved that inhibiting activity of lysyl oxidase (Anti-lysyl oxidase, Anti-LOX) combined with vacuum erectile device (VED) lengthened penis by remodeling tunica albuginea. We thus explored whether HCG supplement could accelerate tunica albuginea remodeling (induced by Anti-LOX + VED) to promote penile growth. Forty-two SD male rats (4 weeks old) were purchased and divided into 7 groups: control, Anti-LOX, HCG, VED (with a negative aspirated pressure of − 300 mmHg), Anti-LOX + VED, HCG + VED, and Anti-LOX + HCG + VED. After an intervention for 4 weeks, all rats’ penile length, exposed penile length, and erectile function were measured. Serum samples were collected to detect hormone levels and penile corpus cavernosum were harvested for histo-pathological analysis. All intervention groups showed significantly longer penis than controlled rats. Anti-LOX sharply increased penile length and exposed length by 15% and 9% respectively, this lengthening effect was more obvious in Anti-LOX + VED group (26% and 19%, respectively). Although HCG promoted penile length by 8%, this effect was slight for exposed length (3%). Moreover, Anti-LOX + HCG + VED dramatically increased penile length and exposed length by 22% and 18%, respectively, which was similar with that in Anti-LOX + VED (26% and 19%, respectively). HCG dramatically stimulated testosterone and dihydrotestosterone secretions than control group, whether with or without Anti-LOX and VED; while it induced more AR expression than other groups. Finally, all procedures did not improve or deteriorate normal erectile function. Although we verified that Anti-LOX + VED lengthened penis by inducing tunica albuginea remodeling, however, HCG supplement did not synergize with Anti-LOX + VED to accelerate albuginea remodeling to facilitate penile growth.
研究领域:
泌尿学
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内分泌学
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性医学
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DOI
PubMed
高影响因子
REPRODUCTIVE BIOMEDICINE ONLINE
·
2015
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IF 3.015
Fenju Qin,Jie Zhang,Linsen Zan,Weiqiang Guo,Jin Wang,Lili Chen,Yi Cao,Ouxi Shen,Jian Tong
The aim of the present study was to elucidate whether the GATA-4/SF-1 signalling pathway is involved in the inhibitory effects of melatonin on testosterone production in both the TM3 Leydig cell line and in C57BL/6J mice. In-vitro experiments demonstrated that melatonin treatment significantly reduced testosterone levels in cell culture medium ( P < 0.05 or P < 0.01); and decreased intracellular cyclic adenosine monophospha accumulation ( P < 0.05 or P < 0.01) and mRNA/protein expression of GATA-4 , SF-1 (NR5A1) , StAR , P450SCC (CYP11A1) and 3β-HSD ( P < 0.05 or P < 0.01). These effects were blocked by N-acetyl-2-benzyltryptamin, a melatonin receptor antagonist . Similar effects of melatonin on testosterone production ( P < 0.05 or P < 0.01) and down-regulation of transcription factors GATA-4 and SF-1 ( P < 0.01) were also observed in mice treated with intratesticular injections of melatonin. Overall, the data suggest that the inhibitory effects of melatonin on testosterone production are mediated via down-regulation of GATA-4 and SF-1 expression.
研究领域:
内分泌学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
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2025
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IF 7.7
Xiang Zuo,Huijuan Bai,Jinyu Qiu,Ruimin Li,Xiaochen Kuang,Yudi Zhao,Jingyi Tuo,Qili Zhao,Xin Zhao,Xizeng Feng
Anxiety Disorder is a common neurological disorder for which ubiquitous environmental endocrine disruptors may be risk factors. 17β-trenbolone (17-TB) has been recognized as a potential environmental endocrine disruptor and its neurotoxic effects have attracted attention. Tachykinin receptor 3 (TACR3), a G protein-coupled receptor involved in pubertal anxiety, and its underlying neural mechanisms remain enigmatic. Therefore, this study investigated the possible relationship between 17-TB and TACR3, testosterone (T), and synaptic plasticity. Our results showed that adolescent male Balb/c mice developed significant anxiety-like behavior after four weeks of exposure to environmentally relevant concentrations of 17-TB (100 μg/kg/day). Transcriptomic RNA-Seq results showed that 17-TB affected abnormal synaptic transmission signals in the hippocampus. Electrophysiological results showed that 17-TB reduced the activity of hippocampal dentate gyrus (DG) neurons and led to the downregulation of hippocampal TACR3 and T levels. In addition, Western blot, immunohistochemistry, ELISA, and qRT-PCR showed that 17-TB exposure led to the downregulation of key hippocampal synaptic proteins PSD95, Gephyrin, and Syn, and induced a metabolic imbalance in glutamate (Glu)/GABA signaling, affecting dopamine signaling. Interestingly, testosterone supplementation (10 mg/kg/day) was effective in ameliorating the above phenomena and alleviating anxiety-like behaviors. These results suggest that 17-TB modulates anxiety-related synaptic plasticity by regulating hippocampal TACR3 and T interactions in vivo. In conclusion, our study contributes to understanding the neurobehavioral and potential mechanistic effects of environmental 17-TB exposure in mammals. It alerts the public to the health risks of chemicals to mammals and suggests new research directions and potential therapeutic targets.
研究领域:
神经科学
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毒理学
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内分泌学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Guangwei Zhao,Shengshuo Zhang,Yujie Zou,Nan Jia,Liyuan Zheng,Jinhua Dong
Introduction: Testosterone is a steroid hormone, which plays a pivotal role in regulating metabolism and protein synthesis in the body. The detection of testosterone is of paramount importance for diagnostic purposes in clinical settings, as well as for monitoring athletes’ physiological parameters and ensuring the integrity of sports competitions.Methods: Herein, we present a phage display-derived biosensing platform through genetic engineering of the TS77 antibody variable domains. The variable region genes of the heavy and light chains from TS77 antibody were cloned into the pDong1 plasmid and displayed on the phage surfaces through phage display technology. Subsequently, a novel non-competitive open-sandwich ELISA (OS-ELISA) was developed for testosterone detection, leveraging the antigen-induced interaction changes in antibody variable regions.Results: OS-ELISA based on anti L-chain antibody achieved a limit of detection (LOD) of 2.71 nM and a half-maximal effective concentration (EC50) of 0.22 μM for testosterone detection. Furthermore, the enhanced OS-ELISA platform incorporating purified maltose binding protein fused with VL (MBP-VL) and VH phage demonstrated a LOD of 1.07 pM and a wide working range from 1 pM to 10 mM.Discussion: The OS-ELISA developed in this study exhibits high sensitivity and a broad dynamic range for testosterone quantification, showing significant potential for clinical diagnostics and athlete monitoring applications.
研究领域:
生物化学
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免疫学
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临床诊断
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DOI
PubMed
高影响因子
ACS Omega
·
2025
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IF 4.3
Huibin Yu,Hongqin Ke,Tu Chen,Jia Huang,Lei Zhou,Shenqi Wang
This study aimed to develop a novel adsorbent, Zn2+-loaded hexanediamine–chitosan (HDA-CS) beads, for the selective removal of human testosterone from plasma. The preparation involved synthesizing chitosan beads, modifying them with hexanediamine to enhance adsorption capacity and subsequently loading them with zinc ions. The effects of the HDA spacer, Zn2+ concentration, and adsorption time on the adsorption percentage for testosterone were systematically investigated through a series of adsorption experiments. Results indicated that the Zn2+-HDA-CS beads achieved a maximum adsorption capacity of approximately 35% within 90 min. The analysis of plasma concentrations of Zn2+, albumin, and sex hormone-binding globulin (SHBG) indicated that the adsorption process involved a complex interaction between testosterone-bound SHBG and albumin for Zn2+ binding sites on the beads. Additionally, the adsorbent demonstrated good storage stability and selectivity for testosterone, with minimal impact on total protein content in plasma. These findings highlight the potential of Zn2+-HDA-CS beads as an effective therapeutic option for managing testosterone levels in clinical settings, particularly in patients undergoing androgen deprivation therapy for prostate cancer.
研究领域:
医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Translational Andrology and Urology
·
2024
·Vol. 13
·期 9
·2055
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IF 1.9
Shoubin Li,Chunhong Yu,Helong Xiao,Qingle Xu,Bo Gao,Liuxiong Guo,Zhanxin Sun,Junjiang Liu
Background Benign prostatic hyperplasia (BPH) is a common disease among older men characterized by non-malignant proliferation of epithelial cells and inflammation. Nitric oxide synthase traffic inducer (NOSTRIN) is a pleiotropic regulator of endothelial cell function and signaling and exerts anti-inflammatory, anti-proliferation, and modulating nuclear factor-kappa B (NF-κB) signaling effects. Its expression and function in BPH tissues and prostate epithelial cells are unknown. The study aims to investigate the expression and functions of NOSTRIN in BPH, and its possible molecular mechanism. Methods The BPH model was constructed in male Institute of Cancer Research (ICR) mice using 5 mg/kg/day testosterone propionate (TP) for 30 days, and the model was evaluated by detecting prostate index, prostate epithelial thickness, and prostate-specific antigen (PSA) expression. Dihydrotestosterone (DHT, 10 nM)-induced in vitro model of human prostate epithelial cells (RWPE-1) was established. We generated lentivirus-harboring human NOSTRIN . The mRNA expression was detected by real-time quantitative polymerase chain reaction (PCR) assay; the protein expression or localization was detected by western blot assay, immunohistochemistry, or immunofluorescence staining. Cell proliferation was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) staining. Reactive oxygen species (ROS) production was observed by dihydroethidium staining. Nitric oxide (NO) and malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were detected using commercial kits. Enzyme-linked immunosorbent assay (ELISA) was used to determine levels of interleukin 1 beta (IL1B), interleukin 6 (IL6), interferon gamma (IFNG), and tumor necrosis factor (TNF). Results NOSTRIN expression was significantly inhibited in the TP-induced ICR mouse BPH model and DHT-induced model of RWPE-1 proliferation. Protein expression of the BPH-related and proliferation markers PSA and proliferating cell nuclear antigen (PCNA) was suppressed in NOSTRIN-overexpressing RWPE-1 cells exposed to DHT. NOSTRIN overexpression notably inhibited the RWPE-1 cell proliferation in vitro , as evidenced by MTT and EdU staining. NOSTRIN overexpression significantly decreased the expression of cell cycle-related proteins cyclin dependent kinase 4 (CDK4) and cyclin D1 (CCND1) in vitro . The production of ROS, NO, and lipid peroxidation products MDA was inhibited by NOSTRIN overexpression in vitro , while the SOD activity was increased. NOSTRIN overexpression reduced the mRNA expression of inflammatory mediator nitric oxide synthase 2 (NOS2) and inhibited the mRNA expression and secretion of pro-inflammatory cytokines IL1B, IL6, IFNG, and TNF in vitro . The mechanistic studies revealed an increased phosphorylation of NF-κB p65 in vivo and in vitro . Remarkably, NOSTRIN overexpression notably inhibited the protein expression of phospho-NF-κB p65 in vitro . Conclusions NOSTRIN is involved in BPH by inhibiting proliferation, oxidative stress, and inflammation in prostate epithelial cells. These functions may act through the inhibition of NF-κB signaling.
研究领域:
泌尿学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Reproductive Biology and Endocrinology
·
2025
·Vol. 23
·期 1
·1-15
·
IF 4.7
Sun Lu,Huang Jiayu,Wang Xuezi,Huang Peng,Dong Baolin,Liang Zehang,Wu Jiahong,Wang Jiancheng
Background Testosterone deficiency (TD) occurs most frequently in older men and can cause many health problems. Testosterone replacement therapy (TRT) is widely used to treat TD, but this regimen can lead to a series of side effects. Stem cell therapy has been wildly studied in vitro. However, due to the multidirectional differentiation potential and heterogeneity of stem cells, it is difficult to achieve the good efficiency and reproducibility in basic research and clinical applications. This study aims to identify a new strategy for the treatment of TD. Methods Bone marrow-derived mesenchymal stromal cells (BMSCs) were enucleated by Ficoll density gradient centrifugation. The organelles and cellular functions of enucleated BMSCs were analyzed by immunofluorescence staining and flow cytometry. Extracellular vesicles (EVs) were isolated by ultracentrifugation and characterized. For the animal studies, enucleated BMSCs were labelled with Mitotracker and injected into ethane dimethanesulfone (EDS)-treated rats. Testosterone production and spermatogenesis were detected at different time points through various tests. To determine the mechanism of efferocytosis, we analysed the number of macrophages by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results The injection of enucleated BMSCs (Cargocytes) into the testes of EDS-treated rats restored the levels of serum testosterone, increased the number of Leydig cells (LCs), and improved spermatogenesis. We found that enucleated BMSCs underwent apoptosis earlier than BMSCs did. Subsequently, testicular interstitial macrophages phagocytosed apoptotic enucleated BMSCs through efferocytosis. Efferocytosis promoted macrophage polarization from the M1 to the M2 phenotype, reduced the expression of proinflammatory cytokines, and decreased the levels of inflammation and oxidative stress. Conclusions In summary, this study pioneered the application of stromal cell enucleation technology to repair tissue damage in the reproductive system, explored the potential of cell burial in the treatment of reproductive system diseases and provided a new approach for the clinical treatment of male infertility.
研究领域:
生殖医学
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再生医学
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免疫学
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细胞生物学
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泌尿外科学
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男科学
·
干细胞研究
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DOI
PubMed
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2018
·
IF 3.026
Huibin Yu,Hongqin Ke,Tu Chen,Xing Li,Shenglong Tan,Lei Zhou,Shenqi Wang
Hemoperfusion using metal ion affinity adsorbent is a promising method to remove human testosterone in plasma. Due to the leakage of metal ion from the adsorbents, there is no metal ion affinity adsorbent for hemoperfusion. In this study, chitosan was used to coat the adsorbent for preventing the leakage of Zn2+ loaded. Meanwhile, freeze-drying method was used to enhance adsorption capacity of Zn2+-loaded cellulose beads for testosterone. The results indicate that after the adsorbent was coated by 0.02% chitosan solution, the highest adsorption percentage reached 48%, during adsorption, the Zn2+ concentration in plasma did not increase; the adsorption capacity of the adsorbent can be significantly enhanced by freeze-drying. The results may be caused by porosity of the adsorbent enlarged via freeze-drying and improved stability by coating with chitosan. In addition, the adsorbent shows better selectivity and storage stability and could be a potential adsorbent to treat prostate cancer.
研究领域:
医学
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）