JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2021
·Vol. 21
·期 9
·4586-4595
·
IF 0
Wang Yun,Chen Zhangjian,Chen Shi,Zhuo Lin,Zhao Lin,Jia Guang
Titanium dioxide nanoparticles (TiO2 NPs) as food additives were widely found in various foodrelated products, especially in high-sugar foods. The daily intake of TiO2 NPs in the diet may therefore expose the small intestine to TiO2 NPs and affect its
physiological functions, including the absorption of nutrients. It is speculated that TiO2 may cause serious health hazards by increasing sugar uptake. To explore this possibility, transport of glucose from small intestine was studied using an everted gut sac model prepared from
small intestine of young healthy male SD rats. The translocation of TiO2 NPs and the morphological changes of small intestine were also observed after exposure of intestinal lumen to TiO2 NPs for 2 h. The results showed that TiO2 NPs can enter into enterocyte
but hardly cross the intestinal epithelium. No change on microstructure of gut epithelia and expression of glucose transporter was found, and there is no obvious impact on intestinal absorption and metabolism of glucose. These results suggest that short-term exposure to TiO2 NPs
has little influence on intestinal absorption of glucose. More attention should be paid to the chronic effect of dietary consumption of TiO2 NPs on nutrient absorption.
研究领域:
纳米技术
·
营养科学
·
毒理学
✓ 已验证
DOI
PubMed
Food Science & Nutrition
·
2024
·
IF 3.5
Jia Hao,Shaohui Beng,Zifeng Ma,Hongmei Xu,Ting Yang,Qiman Yang,Yunduan Wang,Wenhui Zheng,Yisha Ma,Shuo Zhang,Liangde Kuang,Wei Fu
This study aimed to investigate the effects of short-term exposure of Bisphenol A (BPA) on the growth and lactation performance, blood parameters, and milk composition of lactating rabbits and explore its potential molecular mechanisms. Eight lactating rabbits with similar body weight were selected and randomly divided into the experimental group (BPA) and the control group (Ctrl). The group BPA was orally administered 80 mg/kg/day BPA on the 15th day postpartum, while the group Ctrl received a corresponding volume of vehicle. Blood and milk samples were collected after 7 days treatment. The results showed that short-term ingestion of BPA did not obviously alter the body weight, feed intake, or milk yield of the lactating rabbits. ELISA assays indicated that BPA did not significantly affect the plasma levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), creatinine (CRE), alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid (UA), and urea. Utilizing untargeted metabolomics, we first depicted the metabolomic profile of rabbit milk, and identified 277 differential metabolites (DMs), with 141 DMs upregulated (e.g., BPA, and its metabolites including Cetirizine N-oxide) and 136 DMs downregulated (e.g., Oleamide, Tiglic acid, PC O-38:4) in the group BPA. KEGG analysis revealed that the DMs were mainly enriched in pathways comprising fatty acid metabolism, fatty acid degradation, and phosphatidylinositol signaling system, emphasizing the effect of BPA on milk fat metabolism. Hence, we established the BPA-induced MAC-T model, and the results showed that BPA significantly reduced cell viability and impacted lipid synthesis, as evidenced by reduced lipid droplets (BODIPY and Oil Red O staining) and decreased expression of genes related to lipid synthesis (e.g., PPARγ , ACACA , LPL ). In summary, we first drew the metabolomic profile of rabbit milk and confirmed that short-term BPA exposure impacted mammary lipid synthesis, thereby reducing the milk quality of lactating rabbits and providing fundamental data for resolving the toxicological mechanisms of BPA on mammal lactation.
研究领域:
毒理学
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动物科学
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代谢组学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
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