CHINESE JOURNAL OF POLYMER SCIENCE
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2021
·Vol. 39
·期 12
·1538-1549
·
IF 3.603
Lin Sha,Sun Hui,Cornel Erik Jan,Jiang Jin-Hui,Zhu Yun-Qing,Fan Zhen,Du Jian-Zhong
Dented nanospheres show promising potential in drug delivery, nanomotors, etc . However, it is still challenging to prepare them by homopolymer self-assembly because of the strict structural requirements of the homopolymer. Herein, we propose a strategy for preparing dented nanospheres from homopolymers by co-assembly with a short peptide. They were co-assembled from poly(2-hydroxy-3-((4-(ethoxycarbonyl)phenyl)amino)propyl methacrylate) (PHBzoMA 59 ) and ( S )-2-(( S )-2-((((9 H -fluoren-9-yl)methoxy)carbonyl)amino)-3-phenylpropanamido)-3-phenylpropanoic acid (Fmoc-FF-OH). PHBzoMA homopolymers can only self-assemble into nanospheres without dent, and the addition of a short peptide introduced hydrogen bonding and complementary π-π stacking interactions led to the final dented nanosphere morphology. The weight fractions of the short peptide can be adjusted to regulate the final morphology. It was confirmed that the radius of curvature of the dent on the surface was related to the organic bubble inside the protospheres prepared at critical aggregation concentration (CAC). The organic bubble can be adjusted by altering the kind of organic solvent and solution pH, which allowed control over the dented nanosphere dimension. The use of different organic solvents with various polarities allows adjustment of the interfacial tension, and hence the denting degree. This degree can also be controlled by manipulating the solution pH to (de)protonate the short peptide and homopolymer. Furthermore, the versatility of this method was highlighted by using a different homopolymer and the applicability of the resulting dented nanospheres was demonstrated by decoration with gold nanoparticles. Overall, this study provided important insights and a new simple strategy to prepare dented nanospheres in a controlled fashion.
研究领域:
材料科学
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生物化学
·
纳米技术
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DOI
高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2025
·
IF 2.4
Genpei Zhang,Xilong Zhang,Kai Yue,Weishen Zhong
Liposomes serving as nanocarriers offer significant advantages in drug delivery for tumor treatment. There still exists challenges in controlling drug release by disintegrating the liposome membrane for the improvement of therapeutic efficiency. In this paper, a novel method involving the mixture of short peptides with pH-responsive characteristics into the cargo has been introduced. This approach facilitates the release of doxorubicin (DOX) in the acidic tumor tissue environment. The efficacy of this improvement was elucidated through molecular dynamics simulations and experiments. Liposomes incorporating a 1:1 ratio of peptides–DOX exhibited pronounced pH sensitivity and an enhanced drug release profile. The underlying mechanism is attributed to the peptides entering tumor tissues and undergoing protonation in acidic conditions, which increases the hydrophilicity of the peptide–DOX clusters and the internal surface tension of the liposomes. This alteration disrupts the balance between the inner and outer surface tensions of the nanocarrier, causing the liposomes to structurally disintegrate and thus enhancing drug release. The results from both thermodynamic analysis results and experimental data confirm the augmented drug release efficiency of this method, offering valuable theoretical insights for nanoparticle design and determining the optimal mixing ratio for therapeutic applications.
CAS / MDL:
研究领域:
药剂学
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生物医学工程
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Xiangyi Liu,Lulu Du,Jia Liu,Yueting Shi,Qipeng Liu,Ying Xu,Yingying Xia,Xiaiting Wang,Dan Ding,Xingyi Li,Deqing Lin
With the advantages of less invasiveness and better shape adaptability, in situ-forming hydrogels are desired biomaterials as scaffolds, drug carriers, and so on. Herein, a negatively charged NaCl-responsive ultrashort peptide sequence (EEH) is reported whose electrostatic repulsion can be reduced through the charge-shielding effect. Under physiological conditions, its AIEgen-capped amphiphile TPE-GEEH of low concentration (1 mg/mL) presents NaCl-triggered morphological transformation from micelle to closely packed fiber with enhanced emission, which can be applied to biosense sodium ion (Na+) with high sensitivity and quick response. At a slightly acidic pH, 10 mg/mL TPE-GEEH undergoes sol–gel transition upon addition of NaCl (100 mM) with improved mechanical properties, which should be useful to develop an in situ-forming hydrogel. Overall, our report provides a simple strategy to construct NaCl-responsive assemblies for potential application in biosensors and drug delivery system.
研究领域:
生物材料
·
分子生物学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Feng Gao,Jun Guo,Shihao Liu,Feng Zhang,Yi Zhang,Liping Wang
The current state of drug delivery systems allows for the resolution of specific issues like inadequate solubility, limited targeting capabilities, and complex preparation processes, requiring tailored designs for different drugs. Yet, the major challenge in clinical application lies in surmounting these obstacles with a universal carrier that is effective for a variety of anticancer drugs. Herein, with the help of computer simulation, we rationally design ultrashort peptides GY and CCYRGD, which can co-assemble with hydrophobic anticancer drugs into nanoparticles with enhanced solubility, targeting ability and anticancer efficacy. Taking 7-ethyl-10-hydroxy camptothecin (SN38) as a model anticancer drug, the co-assembled SN38-GY-CCYRGD nanoparticles significantly enhance the water solubility of SN38 by more than three orders of magnitude. The as-prepared nanoparticles can effectively kill cancer cells, e.g., human small cell lung cancer (A549) cells with a notable cell mortality rate of 71%. Mice experimental results demonstrate the nanoparticles’ efficient targeting capability, marked reducing the toxicity to normal tissues while improving antitumor efficacy. This work presents a novel drug delivery method, integrating effective, targeted, and safe strategies into a comprehensive carrier system, designed for the administration of hydrophobic anticancer drugs.
研究领域:
药物递送系统
·
肿瘤学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Yamin Xiong,Lihong Zhou,Xiaoxia Peng,Huiling Li,Hui Wang,Leiliang He,Peili Huang
Convenient and rapid detection of Cu 2+ in serum has important clinical significance for related diseases. Herein, a ratiometric detection of Cu 2+ based on specific short peptide ( SPGH )-assisted enhanced chemiluminescence resonance energy transfer (CRET) has been constructed. An interesting phenomenon that tetra-peptide with the sequence of Ser-Pro-Gly-His ( SPGH ) was able to specifically and remarkably enhance the catalytic performance of Cu 2+ has been discovered, the enhancement mechanism is mainly due to the significant increasing generation of Cu 2+ -induced hydroxyl radical after addition of SPGH . The chemiluminescence (CL) of the luminol-H 2 O 2 system was efficiently catalyzed by the formed Cu( SPGH ) 2 complex, which acts as an internal light source to triggers on the fluorescence of QDs via CRET, allowing the ratiometric detection of Cu 2+ within 15 min with improved selectivity and sensitivity. The relative standard deviation was lower than 6.38 % due to the ratiometric signal improving its anti-interference ability. Importantly, it is feasible to detect total Cu in human serum, and the results were consistent with that of ICP-AES ( r = 0.9321, n=55). The reliable, rapid, yet simple ratiometric detection of Cu 2+ in serum based on SPGH -assisted enhanced CRET provides a promising potential alternative method for Cu 2+ detection in clinical application.
研究领域:
生物化学
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分析化学
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临床医学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 19
·3134-3142
·
IF 5.047
Can Wu,Jing Liu,Bin Liu,Suyun He,Guoru Dai,Bo Xu,Wenying Zhong
The design of light-responsive peptide hydrogels with controllable drug release characteristics is still a challenge. Here, we fabricated a NIR light-responsive short peptide hydrogel by using a thermo-responsive short peptide hydrogel and PEGylated NbSe2 nanosheets, namely, the NbSe2–PEG@YD hydrogel. Under NIR light irradiation, the hydrogel began to collapse owing to the local heating, leading to the release of the cargos. The hydrogel exhibited recoverability and the release rate of the cargos could be accurately modulated by the NIR light irradiation according to the requirement. The excellent biocompatibility of this hydrogel ensured that it could be further developed as a drug carrier for clinical application.
研究领域:
生物材料
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药剂学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2014
·Vol. 32
·期 2
·123-127
·
IF 1.04
Dongxia Li,Yang Shi,Ling Wang
We demonstrated in this study that mixing two short peptide-based gelators with different aromatic capping groups would result in molecular hydrogels with enhanced mechanical property. We selected gelators of PTZ-GFFY and Nap-GFFY for the study. Both PTZ-GFFY and Nap-GFFY could independently form molecular hydrogel by a heating-cooling process. However, the mechanical property of gels of PTZ-GFFY and Nap-GFFY was relatively weak with storage moduli ( G′ ) of about 500 and 150 Pa, respectively. A two-component gel of PTZ-GFFY and Nap-GFFY could also form by a heating-cooling process. Surprisingly, the G′ value of the two-component gel was about 5000 Pa , which was at least ten times bigger than that of each single-component gel. This is a novel and simple strategy to improve the mechanical property of molecular hydrogels.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Rui Li,Xianbin Mei,Xiangfeng Li,Changli Zhang,Liping Ruan
A novel bolt-like-blocking nanovalve based on mesoporous silica nanoparticles (MSNs) has developed as a drug carrier, using the modified short peptide as a support and the delivery drug as a bridge for fulfilling the blocking process. The peptide-modified nanoparticle MSN-S-S-NAC-Trp combined with the loaded drug can be used as a carrier with no leakage and be triggered for release by appropriate GSH. The peptide-modified nanoparticles have showed no obvious cytotoxicity and can be internalized into L929 cells; moreover, the doxorubicin loaded nanoparticles incubated with HeLa cells have showed GSH-responsive capability and induced cell apoptosis. Since the chain of the modified peptide on the surface of MSN is not long enough to block the channel via self-assembly process, a bolt-like-blocking model is proposed.
研究领域:
纳米医学
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药物递送系统
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Yu-Ming Fang,Qi-Lei Zhang,Dong-Qiang Lin,Shan-Jing Yao
Short peptide biomimetic chromatography technology as a developing protein separation technology has huge potential for antibody purification. In this study, four tetrapeptide ligands (Ac-FYKH, Ac-YEHF, Ac-YFLH and Ac-FYHI) with high potential binding ability to antibody were selected for the optimal ligand to antibody purification. The results showed that Ac-YEHF-4FF resin had higher binding capacity and selectivity for hIgG among the four resins. And at pH 7.0 and 0.3 ml/min, the highest Q 10%-hIgG of Ac-YEHF-4FF resin was 26.2 mg/ml resin while its Q 10%-BSA was just 2.2 mg/ml resin. Further, Ac-YEHF-4FF resin was used to purify protein mixtures. By binding at pH 7.0 and being eluted at pH 5.0 and pH 4.0, Ac-YEHF-4FF resin was well used to separate hIgG from BSA containing feedstock, HSA containing feedstock and human serum with the purity and yield both more than 95 %. And the screened resin could also separate mAb from CHO cell culture supernatant with purity 94.3 % and yield 97.5 %. The adsorption and separation results of Ac-YEHF-4FF resin indicated that the goal of getting the efficacy of critical residues from protein A to biomimetic its structure and function could be achieved, which had great significance to the establishment and improvement of tetrapeptide biomimetic chromatography, and also provided a new method for the field of antibody separation and purification.
研究领域:
生物化学
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蛋白质工程
·
分离技术
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产品关联
DOI
PubMed
高影响因子
Gels
·
2021
·Vol. 7
·期 4
·206
·
IF 4.702
Yu Guo,Jie Gu,Yuxin Jiang,Yanyan Zhou,Zhenshu Zhu,Tingting Ma,Yuanqi Cheng,Zongzhou Ji,Yonghua Jiao,Bin Xue,Yi Cao
Poly(ethylene glycol) (PEG)-based synthetic hydrogels based on Michael-type addition reaction have been widely used for cell culture and tissue engineering. However, recent studies showed that these types of hydrogels were not homogenous as expected since micro domains generated due to the fast reaction kinetics. Here, we demonstrated a new kind of method to prepare homogenous poly(ethylene glycol) hydrogels based on Michael-type addition using the side chain amine-contained short peptides. By introducing such a kind of short peptides, the homogeneity of crosslinking and mechanical property of the hydrogels has been also significantly enhanced. The compressive mechanical and recovery properties of the homogeneous hydrogels prepared in the presence of side chain amine-contained short peptides were more reliable than those of inhomogeneous hydrogels while the excellent biocompatibility remained unchanged. Furthermore, the reaction rate and gelation kinetics of maleimide- and thiol-terminated PEG were proved to be significantly slowed down in the presence of the side chain amine-contained short peptides, thus leading to the improved homogeneity of the hydrogels. We anticipate that this new method can be widely applied to hydrogel preparation and modification based on Michael-type addition gelation.Keywords:hydrogel;reaction kinetics;mechanical homogeneity;peptide
研究领域:
材料科学
·
生物材料
·
组织工程
·
细胞培养
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·791
·
IF 12.6
Qiao Wenyu,Yang Luling,Liu Yu,Zhang Huaiyuan,Zheng Zhihuan,Gu Tianheng,Zhu Jiaxi,Hu Xinyi,Li Gangze,Zhang Yuxia,Xiao Meng,Zhang Lin
Excessive accumulation of reactive oxygen species (ROS) and dysbiosis of the gut microbiota are pivotal contributors to the pathogenesis of inflammatory bowel disease (IBD) and associated fibrosis. In this study, a microfluidic approach was utilized to fabricate a multifunctional therapeutic microsphere system by encapsulating hollow mesoporous cerium oxide nanoparticles (HCeO 2 ) loaded with KP1 short peptides-specific inhibitors of the TGF-β/Smad signaling pathway. The resulting composite formulation (KP1@HCeO₂@SAM) was designed to achieve synergistic antioxidant, anti-inflammatory, and antifibrotic effects. The developed microspheres exhibited prolonged intestinal retention and effectively modulated gut microbiota composition, increasing the relative abundance of probiotic species by over sevenfold. Moreover, they demonstrated remarkable reactive oxygen species (ROS) scavenging efficiency (> 95%) and significant inhibition of fibrotic signaling cascades. In dextran sulfate sodium (DSS)-induced murine colitis models, treatment with the microspheres led to a substantial reduction in Disease Activity Index (DAI) (> 76%), restoration of intestinal barrier integrity (> 90%), and mitigation of fibrotic progression, as indicated by a 66% decrease in α-SMA expression. These findings establish a novel and integrative therapeutic platform for the effective management of IBD. Graphical abstract
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2017
·
IF 5.246
Jing Xu,Xiangyi Li,Xiaodong Li,Bao Li,Lixin Wu,Wen Li,Xiaoming Xie,Rong Xue
Peptide assembly has reached exquisite levels of efficiency in the creation of bioactive materials. However, we have not yet been able to take what we have learned from peptide assembly to develop a general strategy for the fabrication of biomimetic underwater adhesives, which retain significant advantages as medical glue for clinical treatment. Herein we report a simple approach to prepare peptide-based adhesives through the supramolecular polymerization of cationic peptides drove by polyoxometalates (PMs). Mass spectra, Fourier-transform infrared spectra and 183W NMR spectra confirmed the structural integrity of peptides and PMs during the coassembly process. Scanning electron microscopy demonstrated that the multivalent interactions between peptides and polyoxometaltes led to the formation of robust 3D network structures. The rheological study revealed that the peptide/PM assemblies exhibited mechanically rigid gel-like behavior and self-healing property. Interestingly, the assemblies showed the capacity to adhere various wet solid materials under waterline. The shear strength of the peptide-based adhesives are stronger than that of the commercially available fibrin glue. This finding is exciting and serves to expand our capability of the fabrication of peptide-based materials.
研究领域:
材料科学
·
生物材料
·
化学
·
生物医学工程
·
高分子科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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