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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2016 · IF 3.138

Physical stability of R-(+)-Limonene emulsions stabilized by Ulva fasciata algae polysaccharide

Ping Shao,Huiling Ma,Qiang Qiu,Weiping Jing

The physical stability of R-(+)-Limonene emulsions stabilized by Ulva fasciata polysaccharide (UFP) was investigated in this study. Emulsion physical stability was evaluated under different polysaccharide concentrations (1%–5%, wt/wt) and pH values (3.0–11.0). The stability of R-(+)-Limonene emulsions was demonstrated by droplet size distribution, rheological properties, zeta potential and visual phase separation. R-(+)-Limonene emulsions displayed monomodal droplet size distributions, high absolute values of zeta potential and good storage stability when 3% (wt/wt) UFP was used. The rheological properties and stability of R-(+)-Limonene emulsions appeared to be dependent on polysaccharide concentration. The emulsion stability was impacted by pH. Higher zeta potential (−52.6   mV) and smaller mean droplet diameter (2.45   μm) were achieved in neutral liquid environment (pH 7.0). Extreme acidity caused the flocculation of emulsions, which was manifested as phase separation, while emulsions were quite stable in an alkaline environment. Through comparing the stabilities of emulsions stabilized by different emulsifiers (i.e. UFP, GA and Gelatin), the result suggested that UFP was the best emulsifying agent among them.

CAS / MDL: 5989-27-5
研究领域: 食品科学 · 生物化学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2017 · IF 3.671

Chemical Stability and in vitro release properties of β-carotene in emulsions stabilized by Ulva fasciata polysaccharide

Ping Shao,Qiang Qiu,Jie Xiao,Yueqiong Zhu,Peilong Sun

The aim of the present study was to examine how factors (pH, temperature, iron chelators, and free radical scavengers) influence on the chemical stabilities of β-carotene encapsulated by Ulva fasciata polysaccharide (UFP)-stabilized emulsions and the bioaccessibility under in vitro oral-gastro-intestinal digestion conditions. The concentration of β-carotene in emulsions under different environments was determined. Changes in droplet properties, particle size and microstructure, throughout the oral-gastric-intestinal model were measured and the rate and extent of lipid digestion were recorded in the simulated small intestine fluid. Our results suggested that the pH had a significant effect on the stability of β-carotene, a rapid degradation at acidic environment and a relatively higher stability at high pH (6.0 and 7.0) were observed. β-carotene in emulsions was highly sensitive to extreme temperature (80   °C, 90   °C, and 100   °C). Addition of EDTA or α-tocopherol significantly increased the stability of β-carotene. Meanwhile, digested UFP-stabilized emulsions showed higher lipid digestion rate than GA-stabilized emulsions and BP-stabilized emulsions. UFP was capable of increasing the bioaccessibility of β-carotene in emulsions compared to GA and BP. Therefore, UFP-stabilized emulsion is a promising delivery system to promote applications of β-carotene in functional food and beverage system.

CAS / MDL: 7235-40-7 8001-22-7
研究领域: 食品科学 · 营养学
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY · 2025 · IF 1.6

Sustainable removal of U(VI) by micron-engineered Ulva prolifera: adsorption behavior and mechanism

Chen Yanan,Sui Wenxuan,Peng Jianbiao,He Xindi,Zhu Kairuo

In this work, through the ball-milling treatment process, the novel micron-engineered Ulva prolifera (m- Ulva ) was prepared and used to remove U(VI) from water. The m- Ulva properties were interpreted by analysis of the morphology structure, specific surface area, functional groups, and composition. The adsorption experimental results testified that the U(VI) adsorption process depends strongly on pH, while followed the pseudo-second-order kinetic model and the Langmuir adsorption isotherm model. The maximum absorption capacity was shown to be 147.06 mg/g at pH 5.0 and 25° C. Based on various characterization, the related adsorption mechanisms mainly encompassed electrostatic attraction, complexation and partial reduction of U(VI). The study presents a green, scalable method to transform Ulva prolifera into low-cost, micron-engineered biomass material for U(VI)-contaminated wastewater cleanup.

研究领域: 环境科学 · 海洋生物学 · 生物修复 · 核化学 · 材料科学
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STAR Protocols · 2024 · IF 1.3

Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model

Xiaoxiang Gao,Yinghui Qiu,Wei Liao,Chao Zhao

Summary Regulating cancer-related microRNAs (miRNAs) may become a new generation of therapeutic modalities for cancer treatment. Here, we describe a protocol based on hepatoma-22 (H22) tumor-bearing mice to screen potential targets for treating hepatocellular carcinoma (HCC). We detail the construction of H22 tumor-bearing mice and treatment with two natural compounds, Ulva lactuca L. polysaccharide (ULP) and 5-fluorouracil (5FU). We further describe the isolation of the tumor tissues for miRNA sequencing and the discovery and validation of potential miRNA gene targets against HCC. For complete details on the use and execution of this protocol, please refer to Qiu et al. 1

CAS / MDL: 51-21-8
研究领域: 肿瘤学 · 分子生物学 · 遗传学 · 药理学
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