Jiayuan Zhang,Tian Tian,Shanshan Tian,Jinhai Yao,Yingwan Zhang,Rujia Xie,Ting Yang,Bing Han
Purpose This study aims to investigate the mechanism of action of arsenic-based agents against hepatocellular carcinoma (HCC) and to identify effective drug targets for HCC treatment.Methods Huh7 and HepG2 cells treated with NaAsO2 were assessed for cell viability, pyroptosis, migration, and invasion after undergoing lentiviral transfection. An orthotopic liver tumor model was established and divided into a model group and a treatment group. Proteins associated with QRICH1, PRMT1, cGAS-STING, and the classical pyroptosis pathway were quantified using Western blotting. The intracellular expression and localization of PRMT1 and NLRP3 in HCC were analyzed through cellular immunofluorescence. Co-immunoprecipitation (Co-IP) was performed to examine the protein interactions between PRMT1 and cGAS, as well as between STING and NLRP3. Chromatin immunoprecipitation (ChIP) was used to confirm QRICH1 enrichment in the PRMT1 promoter region.Results NaAsO2 treatment significantly inhibited the proliferation of Huh7 and HepG2 cells and effectively blocked their migration and invasion capabilities, while promoting cellular pyroptosis. Quantitative polymerase chain reaction(QRCR) and ChIP assays confirmed that NaAsO2 regulates PRMT1 expression by down-regulate QRICH1 binding in the PRMT1 promoter region. Additionally, NaAsO2 decreased the expression of the QRICH1-PRMT1 complex and upregulated the cGAS-STING signaling pathway, activating the downstream NLRP3-dependent classical pyroptosis pathway. Overexpression of QRICH1 reversed these effects.Conclusion NaAsO2 inhibits the expression of the QRICH1-PRMT1 axis, activates cGAS-STING signaling pathway transduction, and induces pyroptosis in HCC cells, thereby increasing the infiltration of immune cells in liver cancer tissues.
Nonferrous smelting wastewaters containing high levels of arsenic and sulfuric acid threaten human health and ecological environments due to insufficient disposal technologies and/or possible leakages during transportation to long-term storage facilities. Chemical precipitation is one important route for arsenic removal and immobilization via the formation of arsenic-stabilized minerals. In this study, we propose a new strategy for the treatment of wastewaters containing high levels of arsenic using pyrite as an in situ S and Fe source to prepare environmentally friendly scorodite and As 2 S 3 precipitates. Dissolved pyrite release (S-II) and Fe (II) ions in highly acidic wastewater, both of which drive arsenic precipitation. Sulfide ions readily react with As(III) to generate the stable As 2 S 3 while Fe(II)/As(III) were oxidized to Fe(III)/As(V) by H 2 O 2 . The reaction between Fe(III) and As(V) took place on the pyrite surface as a nucleation site to form amorphous ferric arsenate that ultimately transformed into crystalline scorodite. Sulfide ions were oxidized to H 2 SO 4 that further promoted pyrite dissolution. Up to 99.4% of arsenic was successfully removed from copper smelting wastewaters with an initial arsenic concentration of 11200 mg/L. The concentration of leached As from the As-bearing precipitate was 1.78 mg/L under optimized conditions, which were: a 1.4 FeS 2 /As molar ratio, an initial solution pH of 2, an H 2 O 2 /As molar ratio of 1%, a reaction temperature of 90 °C, and a reaction time of 12 h. In summary, a prospective process successfully shows arsenic removal and immobilization from smelting wastewater, and further demonstrates the potential for the efficient and affordable disposal of wastewater.
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