NATURE
·
2025
·
IF 48.5
Zhu Yunfeng,Xiao Fang,Wang Yiling,Wang Yufang,Li Jialin,Zhong Dongmei,Huang Zhilei,Yu Miao,Wang Zhirong,Barbara Joshua,Plunkett Christopher,Zeng Mengxue,Song Yiyan,Tan Tian,Zhang Ruibin,Xu Kezhen,Wang Zhongxing,Cai Changjie,Guan Xiangdong,Hammack Scott,Zhang Liang,Shi Zheng,Xiang Fu-li,Shao Feng,Xu Jie
Plasma membrane integrity is vital for nearly all aspects of cell functioning 1 . Mechanical forces can cause plasma membrane damage 2 , but it is not known whether there are large molecules that regulate plasma membrane integrity under mechanical strain. Here we constructed a 384-well cellular stretch system that delivers precise, reproducible strain to cultured cells. Using the system, we screened 10,843 siRNAs targeting 2,726 multi-pass transmembrane proteins for strain-induced membrane permeability changes. The screen identified NINJ1, a protein recently proposed to regulate pyroptosis and other lytic cell death 3,4 , as the top hit. We demonstrate that NINJ1 is a critical regulator for mechanical strain-induced plasma membrane rupture (PMR), without the need of stimulating any cell death programs. NINJ1 level on the plasma membrane is inversely correlated to the amount of force required to rupture the membrane. In the pyroptosis context, NINJ1 on its own is not sufficient to fully rupture the membrane, and additional mechanical force is required for full PMR. Our work establishes that NINJ1 functions as a bona fide determinant of membrane biomechanical properties. Our study also suggests that PMR across tissues of distinct mechanical microenvironments is subjected to fine tuning by differences in NINJ1 expression and external forces.
研究领域:
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Guangyu Ou,Jun Li,Yang Jin,Ming Chen,Yujing Ma,Kaige Gao
Electric fields, which can promote the approach of droplets and break the liquid film, are extensively used in the separation of the water phase in water-in-oil emulsions. However, there is an evolution of droplet behavior under an electric field. After the two droplets meet with each other, the electric force becomes undesirable, which would even cause breakup of the merged droplet. When the electric field strength E reaches a particular value, the final behavior of droplets is made, which goes against coalescence, and there are lots of behavior evolution types. Several research studies have studied on whether droplets coalesce and the critical condition, but few works have focused on the classification and mechanism of non-coalescence behaviors. In this paper, the behavior evolution of two single droplets suspended in castor oil under an alternating current electric field is studied by a high–speed camera. Six distinct behavior evolution modes are observed and summarized: coalescence, bounce, partial coalescence, partial rupture, coalescence–rupture, and rupture. The behavior evolution mode is influenced by the initial separation distance s0 between droplets and the electric field strength. Moreover, there exist critical electric field strengths among different behavior evolution modes. As E gradually increases, two water droplets go through coalescence, partial coalescence, and coalescence–rupture in sequence when s0 is small and coalescence, bounce, partial rupture, and rupture when s0 is large. The mechanisms of behavior evolution are revealed by investigating the confrontation between electric force and capillary force in the condition with liquid bridge or pressure difference from the surrounding fluid and electric force in the condition without a liquid bridge. In addition, a cone–dimple mode of water droplets in castor oil is found, demonstrating the rationality of electric force theory.
研究领域:
物理学
·
流体力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2017
·
IF 4.421
Rui Ma,Taotao Fu,Qindan Zhang,Chunying Zhu,Youguang Ma,Huai Z. Li
This paper investigated the breakup dynamics of ferrofluid droplet in a microfluidic T-junction with the magnetic field. Droplet breakup types with permanent obstruction, part obstruction and without obstruction were observed. The droplet breakup processes with permanent and part obstruction could be divided into squeezing, transition and pinch-off stages. The minimum width of droplet neck could be scaled as power-law relationships with time for squeezing stage and with remaining time for the last two stages. The droplet breakup process without obstruction has only last two stages. The results show that the magnetic field could affect the dynamics of droplet breakup.
研究领域:
流体力学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xiao-Yuan Wang,Jian Lv,Qin Hong,Ze-Rui Zhou,Da-Wei Li,Ruo-Can Qian
H2O2 is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular H2O2, and these methods usually require sophisticated probe synthesis and instruments. In particular, local H2O2 treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under H2O2 treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.
研究领域:
生物医学工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-14
·
IF 16.6
Peng Lu,Zhu Haojie,Wang Haobin,Guo Zhenbin,Wu Qianyuan,Yang Cheng,Hu Hong-Ying
Water disinfection is conventionally achieved by oxidation or irradiation, which is often associated with a high carbon footprint and the formation of toxic byproducts. Here, we describe a nano-structured material that is highly effective at killing bacteria in water through a hydrodynamic mechanism. The material consists of carbon-coated, sharp Cu(OH) 2 nanowires grown on a copper foam substrate. We show that mild water flow (e.g. driven from a storage tank) can efficiently tear up bacteria through a high dispersion force between the nanotip surface and the cell envelope. Bacterial cell rupture is due to tearing of the cell envelope rather than collisions. This mechanism produces rapid inactivation of bacteria in water, and achieved complete disinfection in a 30-day field test. Our approach exploits fluidic energy and does not require additional energy supply, thus offering an efficient and low-cost system that could potentially be incorporated in water treatment processes in wastewater facilities and rural communities.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Asia-Pacific Journal of Chemical Engineering
·
2024
·
IF 1.4
Yixiang Zhang,Shilong Feng,Yuhui Jing,Junhua Bai
High expansion (Hi-Ex) foam is recommended to suppress the leakage and diffusion of cryogenic liquid due to its light weight and large volume. However, the disadvantages of low stability and high break rate under environmental conditions are all limited the further application in vapor mitigation and fire extinguishing. So that, this paper focus on the effect and mechanism of nanoparticles in stabilizing Hi-Ex foam. Three kinds of nanoparticles with different concentration were selected to evaluate the effect of foam half-life and the mechanism of particles on improving the foam stability. The results indicated that different particle concentrations can improve the foam stability to a specific extent, and the maximum improving of half-life can increase by 95.4% in the presence of the hydrophilic SiO 2 at .5 wt%. Meanwhile, the hydrophilicity, size, and morphology of the particles have a specific impact on the foam stability. The foam expansion rate first increased and then decreased. From the microscopic point of view, the bubble size gradually increases with time by two processes of ripening and coalescence and satisfied in a logarithmic distribution. While, the liquid film thickness remarkably decreases due to foam drainage without particles and the adsorption and accumulation of nanoparticles on foam lamella can provide a spatial barrier for the film thinning and the inter bubble diffusion. Finally, the microscopic interaction mechanism on improving the foam stability has been further explored and revealed in these two aspects.
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 43
·2304459
·
IF 19
Haoyu Dai,Linfeng Yang,Yaping Feng,Junhan Sun,Fenglin Chen,Xianfeng Luo,Zengyi He,Xuetao Xu,Bing Wang,Xubo Liu,Zhichao Dong,Lei Jiang
Emulsified oil leakage onto the bulk water surface causes severe issues on global ecology and health. Developing efficient, rapid, and universal separation methods of emulsions has been a significant topic in scientific studies. However, a contactless and additive-free strategy to achieve continuous floating emulsion separation and oil collection remains to be discovered. Herein, a universal contactless demulsification, transportation, and collection method to dispose floating emulsions by ionic wind, which contains active charged particles generated by corona discharge is reported. The splash-like demulsification process of floating emulsions is attributed to the rupture of water film enveloped on oil droplet surface, simultaneously and respectively. The evidence of this process recorded by a high-speed camera with 20 000 fps has a referential significance for other works. This study may clean up universal floating emulsions discharged on water in real situations such as oil stations, chemical plants, and restaurants, and furthermore increase the potential of remote control in liquid dynamics by corona discharge.
研究领域:
环境科学
·
流体力学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Weidong Fang,Zhi Tao,Haiwang Li,Yuqian Ma,Shuai Yin,Tiantong Xu,Teckneng Wong,Yi Huang
Emulsions have been applied in a number of industries such as pharmaceutics, cosmetics, and food, which are also of great scientific interest. Although aqueous emulsions are commonly used in our daily life, oil-in-oil (o/o) emulsions also play an irreplaceable role in view of their unique physics and complementary applications. In this paper, we investigate typical behaviors of organic droplets surrounded by organic medium (o/o emulsions) with different functional groups controlled by the AC electric field. Droplet behaviors can be catalogued into five types: namely, “no effect”, “movement”, “deformation”, “interface rupture”, and “disorder”. We identify the key dimensionless number Wee·Ca, combined with the channel geometry, for characterizing the typical behaviors in silicon oil/1,6-hexanediol diacrylate and mineral oil/1,6-hexanediol diacrylate emulsions. Unlike aqueous emulsion, the Maxwell–Wagner relaxation inhibits the electric effect and leads to an effective frequency, ranging from 0.5 to 3 kHz. The increasing viscosity of the droplet facilitates the escalation by promoting the shearing effect under the same flow conditions. Ethylene glycol droplets primarily show the efficient coalescence even at a low Wee·Ca, which is attributed to the attraction of free charges induced by the increasing conductivity. In 1,6-hexanediol diacrylate/silicon oil emulsion, the droplet tends to form a liquid film that expands into the entire channel due to the affinity of the droplet to the channel wall. A variety of elongated columns are observed to oscillate between the electrodes at high voltages. These findings can contribute to understanding the electrohydrodynamic physics in o/o emulsion and controlling droplet behaviors in a fast response, programmable, and high-throughput way. We expect that this droplet manipulation technology can be widely adopted in a broad range of chemical synthesis and biological and material science.
研究领域:
物理学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MECHANICAL SCIENCES
·
2022
·
IF 6.772
Yiheng Sun,Guijing Dou,Kang Wu,Peijian Chen,Taihua Zhang,Guangjian Peng
Characterization of the rupture behavior is of great significance to evaluate the service life for microcapsules. Due to the small dimension, complex core-shell structure and curved surface of microcapsules, it is challenging to quantitatively determine the rupture position and rupture strength. In the present work, a microsphere-compression model for characterizing the rupture behavior of microcapsules was established. Through the quantitative investigation of the compression rupture behaviors of microcapsules from the extended finite element models, the cracks were found to initiate at the same parallel circle (latitude) of microcapsule where the curvature change of the deformed shell (i.e., prime vertical strain) is the largest. With the increase of compression displacement, microcapsules show three crack propagation modes. Based on such findings, a maximum prime vertical strain criterion, i.e., cracks initiate when the maximum prime vertical strain exceeds a critical value, for estimating the rupture initiation of microcapsules under compression was proposed. The proposed criterion was validated by both numerical simulations and experiments. It demonstrates that the critical prime vertical strain of rupture is independent of the microcapsule dimensions and is an intrinsic parameter to judge the rupture of microcapsules. The critical prime vertical strain of rupture for n-hexadecane@PMMA microcapsules is 0.023±0.003. The proposed model avoids complicated stress analysis and provides an intrinsic criterion.
研究领域:
材料科学
·
力学
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY AND TECHNOLOGY OF FUELS AND OILS
·
2024
·Vol. 60
·期 2
·309-314
·
IF 0.6
Liu Tianyu,Ye Zhongbin,Liu Dong
Utilizing emulsion polymerization, a microcapsule breaker with a polystyrene-polyacrylamide core-shell structure was synthesized. Ammonium persulfate served as the breaker, polystyrene as the shell, and polyacrylamide as the drug-carrying agent. The results demonstrated that the synthesized microencapsulated gel-breaker exhibited a uniform spherical shape, superior water dispersion, and enhanced thermal stability. Conductivity tests indicated that the core-shell structure of the microcapsules effectively regulated the release of ammonium persulfate as a gel-breaking agent, resulting in delayed polymer gel-breaking.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Wenqi Zhao,Yanjun Deng,Haoyu Feng,Hao Chen,Chunying Zhu,Taotao Fu,Youguang Ma
The breakup behavior and dynamics of nanofluid droplets in the T-shaped microchannel were investigated, focusing on the effect of nanoparticles on droplets breakup. It was found that the solid particle layer formed by the adsorption of nanoparticles at the droplet interface would greatly limit the droplet deformation. Three breakup flow patterns were observed: non-breakup, breakup with partial obstruction, and breakup with permanent obstruction. The droplet breakup could be divided into three stages. In the squeezing stage, the nanoparticles made the droplet breakup slow down, resulting in decrease of power-law exponent α . In the transition stage, the adsorption of nanoparticles changed the surface properties of droplets, reducing the slope k . In the rapid pinching stage, the decrease of nanoparticle concentration or the increase of the dispersed phase capillary number Ca d contributed to a growth in power-law exponent β . This study provides crucial data and technical support for applications of nanoparticle-stabilized emulsions.
研究领域:
流体力学
·
纳米技术
·
微流控技术
✓ 已验证
产品关联
DOI
高影响因子
DESALINATION
·
2020
·
IF 7.098
Peng Du,Xing Li,Yanling Yang,Zhiwei Zhou,Xiaoyan Fan
A novel coagulation strategy, where floc property was regulated by varying dosing sequence of dual coagulants of aluminum sulfate (AS) and chitosan (CS) with floc breakage, was applied as the pre-treatment of ultrafiltration to treat algae-laden water. The river water (TW), and TW spiked with intracellular organic matter (IOM) and extracellular organic matter (EOM) respectively (TW-IOM and TW-EOM) were tested. The results indicate that floc breakage applied in successive dosing of AS and CS in TW and TW-IOM water samples formed compact flocs through charge neutralization and bridging, generating dense cake layer to control irreversible fouling; and mitigated irreversible fouling (R ir ) caused by protein-like and humic-like species in the molecular weight (MW) of 950–3000 Da. For TW-EOM water sample, the successive addition of CS and AS can relieve R ir by removing protein-like species and soluble by-products in the MW of 500–3000 Da via charge neutralization, sweeping flocculation and bridging together, while the applied breakage aggravated membrane fouling . The obtained results provide a novel coagulation strategy to control membrane fouling during algae-laden water treatment .
研究领域:
环境工程
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）