SEPARATION AND PURIFICATION TECHNOLOGY
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2017
·
IF 3.359
Ying Zhao,Xinping Ouyang,Jing Chen,Lisha Zhao,Xueqing Qiu
The conversion of lignin into value-added aromatic monomers has attracted much attention as these monomers can be potentially used to prepare liquid fuel rather than heavy oil by further hydrodeoxygenation. A new process combining Sephadex and silica gel column chromatography was employed to obtain aromatic monomers with higher purity. It was found that pH and polarity of mobile phase were the most important factors affecting the separation effect for Sephadex G-10 and silica gel column chromatography, respectively. When the pH of mobile phase reached 10.5, the ionization of hydroxyl together with carboxyl groups resulted in electrostatic repulsion between the aromatic compounds and Sephadex G-10 gel, which counteracted the adsorption caused by hydrogen bond between aromatics and gel, causing the increase of the separation effect. Under the condition of pH above 10.5, Sephadex gel column chromatography gave 78.1% of purity of aromatic monomers (PAM) and 99.5% of recovery of aromatic monomers (RAM) due to the elimination of non-size exclusion effect. Silica gel column chromatography was further conducted to obtain 99.8% of PAM and 73.9% of RAM. The polar mobile phase was favorable for eluting aromatic monomers, while the non-polar mobile phase was beneficial to remove oligomers and increasing the PAM.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yiwen Wang,Yafei He,Aijun Gong,Lina Qiu,Min Zhang,Mory Traore,Xiaoning Zhan,You Li,Yuzhen Bai,Yang Liu,Ge Gao,Weiyu Zhao
We developed an adsorption column that can be used for rare earth recovery from acid mine drainage (AMD). 2-[2-oxo-2-(1-pyrrolidinyl)ethoxy] acetic acid was used as a functional ligand grafted onto amino silica gel , resulting in an adsorbent selective for rare earth metals . Adsorption columns for the enrichment of rare earth metals have been made using this functional silica. A flow rate of 500 µL min −1 and an eluent of pH= 2 0.013 mol L −1 DTPA were determined as the optimum operating conditions. The recovery of actual AMD using SiO 2 @PYRDGA adsorption column achieved 82.29%, 75.66%, 85.10%, 78.43% for Er, Gd, Nd and La, and the purity of rare earth in AMD can be increased about 10 times after recovery. In addition, the thermodynamics, kinetics, adsorption mechanism, and theoretical calculations of the adsorption process were investigated in detail.
研究领域:
环境科学
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材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Danni Jiang,Jia Chen,Ming Guan,Hongdeng Qiu
In this work, 1-vinyl-3-octadecylimidazolium bromide ionic liquids ([C 18 VIm]Br) and their derived carbon dots (ImC 18 CDs) were prepared, [C 18 VIm]Br and ImC 18 CDs were grafted on the silica to obtain Sil-ImC 18 and Sil-ImC 18 CDs, respectively, and they were also co-grafted on silica which named Sil-ImC 18 /CDs. Compared with Sil-ImC 18 and Sil-ImC 18 CDs columns, Sil-ImC 18 /CDs column exhibited enhanced selectivity for separation of tetracyclic/tricyclic polycyclic aromatic hydrocarbon (PAH) isomers, and butylbenzene isomers in reversed-phase liquid chromatography, which may be due to the synergistic effect between ImC 18 CDs and [C 18 VIm]Br, the π-π interaction between imidazolium and analytes, etc. Meanwhile, the retention behavior of Sil-ImC 18 /CDs was further evaluated and compared with the commercial C 18 column using different classes of analytes, including standard test mixtures of Tanaka, Engelhardt, SRM869b, SRM870. The results demonstrated that co-grafted column exhibited superior separation performance. And this column was applied to determine the contents of calycosin-7-glucoside, ononin , calycosin and formononetin in the extract of Radix Astragali, which were found that the concentration was 0.25 mg mL −1 , 0.15 mg mL −1 , 0.13 mg mL −1 and 0.30 mg mL −1 , respectively.
研究领域:
分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Linfeng Wen,Lixin Yang,Qixin Kan,Peipei Wang,Jun Li,Guo Liu,Liping He,Jiangyan Fu,Qingrong Huang,Chi-Tang Ho,Yong Cao
The taste characteristics of soy sauce are closely related to its chemical composition. In this study, raw soy sauce was analyzed by silica gel column chromatography (SGCC) and the fractions were evaluated by a sensory trained panel. Based on the sensory results, the representative sensory-guided fractions were further analyzed by untargeted metabolomics technology. Moreover, we carried out the correlation between the taste characteristics and chemical composition of these fractions. The yield of umami-taste fractions was 62.50%. Fractions F-2 (eluted by ethyl acetate) and F-4 (eluted by 90% ethanol) were significantly associated with the bitter and acid tastes, and the umami and salty tastes, respectively. They showed better ratings than other fractions. Seventeen compounds were identified through chemical profiling and they were classified into four categories, including amino acids and derivatives, organic acids, carbohydrates and conjugates and the others. It revealed that compounds of different categories in F-2 and F-4 had different correlations with taste characteristics, which provided theoretical research for the study of soy sauce flavor. In conclusion, this study is beneficial to boost product competitiveness in the marketplace, improve industry standardization, and drive technological innovation in the industry.
研究领域:
食品科学
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化学
·
感官分析
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 12
·7974-7983
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IF 3.612
Bailiang Zheng,Yana Ding,Joel B. Johnson,Jinle Xiang,Zhenzhen Li,Youyang Zhang,Denglin Luo
Summary The adsorption and desorption capabilities of eight types of macroporous adsorption resins (MARs) for polyphenols from foxtail millet bran (FMB) extract were investigated. The D101 resin demonstrated the highest adsorption and desorption capacity, followed by the AB-8, DM301 and ADS-17 resins. The reaction kinetics for the adsorption of FMB polyphenols onto MARs accorded with a pseudo-first-order model ( R 2 > 0.99), with an apparent adsorption rate constant (K) of 1.225 × 10 −2 g/mg·min. The adsorption process of FMB polyphenols onto D101 resin could be best described with the Langmuir adsorption isotherm ( R 2 > 0.99). The phenolic fractions were separated using silica gel column chromatography, and their total phenolic content (TPC), antioxidant activity, total flavonoid content (TFC), anti-glycosylation activity and acetylcholinesterase and α-glucosidase inhibitory activities were compared. Fraction 4 had the highest abundance of phenolic compounds, with N′- p -coumaroyl-N″-feruloylspermidine (461.65 ± 0.79 μg/g) and N′,N″-di-feruloylspermidine (578.46 ± 2.56 μg/g) being the predominant phenolics, the best acetylcholinesterase inhibitory activity and anti-glycosylation activity. Fraction 5 contained the highest apigenin- C -dihexoside, and therefore showed the best performance of α-glucosidase inhibitory activity. Fractions 4 and 5 displayed high antioxidant, acetylcholinesterase inhibitory activity and anti-diabetic potential, indicating that these two fractions should be targeted for further high-value utilisation of free polyphenols from FMB.
研究领域:
食品科学
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植物化学
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生物化学
✓ 已验证
DOI
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Lu Huang,Yingjie Guo,Tianqi Jin,Ke Yan,Xianglin Liu,Shuyang He,Lin Li,Ye Gong,Junyi Ma,Hang Yu,Zhoumin Lu,Yupeng Liu,Wuzi Dong,Fangxia Yang
Triterpene acids (TTAs) have promising applications in functional food, medicine and other fields. Here, ultrasonic-assisted hydrophobic deep eutectic solvent (HDES) extraction was employed to gain TTAs from loquat leaves. Combining COSMO-SAC model prediction with experimental verification，the HDES, composed of thymol and benzyl alcohol (molar ratio 2:1), was proved to have the highest extraction ability for TTAs. The preliminarily optimized extraction conditions were solid to solvent ratio 1:10 (g/g), temperature 60 °C and extraction time 60 min. Under these conditions, the extraction yield of ursolic acid (the main component of loquat leaf TTAs) was 17.769 ± 0.799 mg/g dry loquat leaf. HDES and TTAs were recovered from HDES extracting solution via gradient elution (60% and 80% ethanol aqueous solution, respectively) based on C18 reverse-phase silica gel column chromatography under low pressure (0.5 MPa). Recovered HDES could be reused for extracting TTAs from loquat leaves directly and had a similar extraction performance compared to fresh HDES. The purity of the obtained crude TTAs sample was 81.18% based on the total contents of maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and ursolic acid (UA), with a mass ratio of 11:26:10:53. Crude TTAs sample was further separated by C18 reverse-phase silica gel column chromatography using 80% ethanol aqueous solution as mobile phase under high pressure (15 Mpa). By this mean, CA sample with 69.30% purity (MA to CA mass ratio 7:18) and UA sample with 93.94% purity (OA to UA mass ratio 4:21) were obtained. The in vitro antioxidant ability order of the attained TTAs samples was CA sample > crude TTAs sample > UA sample. This study laid a good scientific basis for applying HDES and C18 reverse-phase silica gel column chromatography in the bioactive component extraction and separation fields. It also provided theoretical basis and technical support for obtainment and utilization of high-purity loquat leaf TTAs in the fields of medicine, functional food, cosmetics and so on.
研究领域:
药学
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食品科学
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分析化学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Yanyan Wang,Liuqian Bao,Jiajing Sun,Yuanyuan Ding,Jiasheng Shi,Zhengyu Duan,Zhiyong Chen
Fluorous affinity chromatography has received growing attention in separation and purification of fluoro compounds, but the wettability of the fluorinated stationary phases is seldom noticed. Here, we construct a series of micro-sized fluorine-containing microspheres by solvothermal precipitation polymerization. The fluorinated microspheres could be obtained with narrow size distribution at even high monomer loading of 15 wt%. Through alternating fluoro monomer, both the particle size and the wettability of the microsphere array could be tuned. Among them, the poly(divinylbenzene -dodecafluoroheptyl methacrylate), P(DVB-DFHMA), microsphere (6.1 μm) arrays displays superhydrophobicity with 153.2° water contact angle. The P(DVB-DFHMA) fluorinated microspheres (7.58% fluorine content) can be packed into steel-less columns as stationary phase for high-performance liquid chromatography. The retention mechanism of the fluorinated column is proven to be the specific fluorine-fluorine interaction. Compared to the commercial C18 silica column, the fluorinated column can completely separate fluorine-containing compounds under high water content mobile phase, including small fluoro molecules and fluoro macromolecules, at much lower back pressure by fluorous affinity.
研究领域:
化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）