FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Yiqing Zhu,Xinyang Sun,Xieqi Luo,Jian Ding,Fengjiao Fan,Peng Li,Xinchun Shen,Yong Fang
In this study, nanoparticles (NPs) prepared with xanthan gum and lysozyme were established as a powerful delivery system for two Se-containing peptides: TSeMMM (STP) and SeMDPGQQ (SHP). NPs-STP and NPs-SHP had relatively small particle sizes (145 nm and 148 nm) and negative zeta potentials (−47 mV and −49 mV). The encapsulation efficiency of NPs-STP and NPs-SHP was determined to be 34.35% and 41.35%, respectively. The stability and antioxidant activity of Se-containing peptides were greatly enhanced due to encapsulation. NPs-STP and NPs-SHP exhibited controlled release of Se-containing peptides under in vitro gastrointestinal conditions. NPs-STP and NPs-SHP showed low toxicity and entered Caco-2 cells through clathrin-mediated endocytosis, contributing to a significant increase in the apparent permeability coefficient of STP (2.19 × 10 −6 cm/s) and SHP (2.21 × 10 −6 cm/s). Thus, NPs-STP and NPs-SHP are considered promising delivery systems for Se-containing peptides and have good potential applications in the food and pharmaceutical industries.
研究领域:
食品科学
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Jiayu Zheng,Fengjiao Fan,Wenqian Tang,Nanlong Li,Xiaoyi Jiang,Yong Fang
The TSeMMM and SeMDPGQQ, two selenium-containing peptides derived from Se-enriched rice protein hydrolysates, have previously been demonstrated to show neuroprotective properties against high-dose lead exposure. However, the potential toxicological effects of these functional peptides on low-dose lead-induced neurotoxicity remain poorly understood. The present study aimed to investigate the protective mechanism of two selenium-containing peptides in mitigating low-dose Pb 2+ -induced damage in HT22 cells. Results showed that treatment with 40 μmol/L Pb 2+ significantly reduced cell viability and increased apoptosis, both of which were alleviated by 40 μmol/L TSeMMM and SeMDPGQQ treatment. Furthermore, two peptides exhibited antioxidant potentials by reducing reactive oxygen species levels to 32.19% and 79.29% of the model group, respectively. They also enhanced antioxidant enzyme activities and mitigated oxidative stress through activation of the Keap1/Nrf2 pathway. Additionally, two peptides improved mitochondrial function by elevating mitochondrial membrane potential, ATP production while dramatically reducing reactive oxygen species levels. Interestingly, TSeMMM exhibited superior neurointerventional effects compared to SeMDPGQQ. The RNA sequencing analysis revealed that TSeMMM modulated gene expressions related to oxidative stress and neuroprotective function. The study provides insights into the protective mechanism underlying selenium-containing peptides for mitigating neuronal damage.
研究领域:
神经毒理学
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药理学
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分子生物学
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细胞生物学
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产品关联
DOI
高影响因子
Food Bioscience
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2025
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IF 5.9
Xin Wang,Bailiang Xue,Rong Zhang,Lin Shi,Wei Jia
To enable the precision production and identification of bioactive selenium peptides from selenium-biofortified rice proteins, this study developed an approach combining controlled enzymatic hydrolysis, ultrafiltration, and high-resolution mass spectrometry. Rice proteins were subjected to chymotrypsin/trypsin digestion, followed by ultrafiltration to enrich low-molecular-weight selenium peptides. A selenium-specific peptide database was constructed by systematically replacing methionine (Met) and cysteine (Cys) with selenized forms in UniProtKB Oryza sativa L. protein sequences (195,302 entries), generating 88,176 unique selenium peptide entries to overcome limitations in traditional database annotations. Ultra-performance liquid chromatography coupled with quadrupole-Orbitrap (UHPLC-Q-Orbitrap) and dynamic mass calibration enabled high-confidence screening of selenium peptides under EU 2002/657/EC guidelines, identifying 104 candidate selenium peptides. Among these, 14 high-confidence peptides (coverage score >5 %) were sequenced de novo using mMass 5.5.0. Parallel reaction monitoring (PRM) and multidimensional validation (fragment ion matching and modification site localization) confirmed the structure and bioactivity of key selenium peptides, such as Leu-{Se-Met}-Ala-Lys, which exhibited strong binding affinity to dipeptidyl peptidase IV (DPP-IV) active sites (binding energy: −64.54 kcal mol −1 ). Research findings demonstrate that selenium peptides can be precisely isolated from biofortified crops and characterized for their functional properties. The dynamic database and high-resolution analysis framework can be expanded to include selenium morphology analysis, bioactive peptide discovery and precise nutrition research, promoting the development of data-driven functional food.
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产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
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2022
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IF 9.336
Xieqi Luo,Fengjiao Fan,Xinyang Sun,Peng Li,Tong Xu,Jian Ding,Yong Fang
Rice selenium-containing peptide TSeMMM (T) with immunomodulatory functions was isolated from selenium-enriched rice protein hydrolysates. However, its biological activity is difficult to be protected in complex digestive environments. In this study, T was encapsulated within zein and gum arabian (GA) through ultrasound treatment to improve its bioactivity and bioavailability. The [email protected] /GA nanoparticles were formed using ultrasonic treatment at 360 W for 5 min with a 59.9% T-encapsulation efficiency. In vitro digestion showed that the cumulative release rate of [email protected] /GA nanoparticles reached a maximum of 80.69% after 6 h. In addition, short-term animal studies revealed that the nanoparticles had an effect on the levels of tissue glutathione and improved peptides’ oral bioavailability. Conclusively, these findings suggest that the ultrasonicated polysaccharide/protein system is suitable for encapsulating active small molecular peptides. Furthermore, it provides a novel foundation for studying the bioavailability of active substances in functional foods.
研究领域:
食品科学
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纳米技术
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生物技术
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药学
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产品关联
DOI
PubMed
高影响因子
Redox Biology
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2024
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IF 10.7
Mengfei Chen,Zhenjun Zhu,Shujian Wu,Aohuan Huang,Zhiqing Xie,Jie Cai,Rong Huang,Shubo Yu,Ming Liu,Jumei Zhang,Yuchung Tse,Qingping Wu,Juan Wang,Yu Ding
Oxidative stress (OS) and disruption of proteostasis caused by aggregated proteins are the primary causes of cell death in various diseases. Selenopeptides have shown the potential to control OS and alleviate inflammatory damage, suggesting promising therapeutic applications. However, their potential function in inhibiting proteotoxicity is not yet fully understood. To address this gap in knowledge, this study aimed to investigate the effects and underlying mechanisms of the selenopeptide VPRKL(Se)M on amyloid β protein (Aβ) toxicity in transgenic Caenorhabditis elegans . The results revealed that supplementation with VPRKL(Se)M can alleviate Aβ-induced toxic effects in the transgenic C. elegans model. Moreover, the addition of VPRKL(Se)M inhibited the Aβ aggregates formation, reduced the reactive oxygen species (ROS) levels, and ameliorated the overall proteostasis. Importantly, we found that the inhibitory effects of VPRKL(Se)M on Aβ toxicity and activation of the unfolded protein are dependent on skinhead-1 (SKN-1). These findings suggested that VPRKL(Se)M is a potential bioactive agent for modulating SKN-1, which subsequently improves proteostasis and reduces OS. Collectively, the findings from the current study suggests VPRKL(Se)M may play a critical role in preventing protein disorder and related diseases.
研究领域:
神经科学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Jiaojiao Zhou,Lingfeng Wei,Zhenyu Wang,Zia-ud Din,Xuqin Lv,Yue Gui,Fang Xie,Jie Cai
Selenium-enriched peptide (SP) is a new type of selenium supplement with unique bioactivities; however, its instability jeopardizes its industrial applications. Hence, a system stabilizing and delivering SP is a prerequisite to overcome this defect. This study developed a microcapsule, dextran–whey protein isolate–selenium-enriched peptide (DX–WPI–SP), encapsulating SP within the WPI–DX glycation product via electrospraying technology, and its properties were characterized. Results showed that DX–WPI–SP considerably surpassed the physical mixture of WPI, DX, and SP, with respect to antioxidant capacity, thermal stability, and cell cytotoxicity. The in vitro gastrointestinal digestion results showed that over 42% of the SP in DX–WPI–SP survived the gastric fluids and was then released in the intestinal phase. The stability of SP in long-term storage was also observed after its encapsulation in DX–WPI–SP, signifying its stability in this delivery system. This study demonstrated that the DX–WPI–SP microcapsule is a valid strategy to stabilize SP in intestinal delivery.
研究领域:
食品科学
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营养学
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生物技术
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药学
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产品关联
DOI
高影响因子
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