Nutrients
·
2024
·Vol. 16
·期 11
·1737
·
IF 4.8
Sheng Zhao,Yuzhou Meng,Wenxun Cai,Qiwen Luo,Hongyang Gao,Qiang Shen,Dongyun Shi
Tumor cells are characterized by a delicate balance between elevated oxidative stress and enhanced antioxidant capacity. This intricate equilibrium, maintained within a threshold known as redox homeostasis, offers a unique perspective for cancer treatment by modulating reactive oxygen species (ROS) levels beyond cellular tolerability, thereby disrupting this balance. However, currently used chemotherapy drugs require larger doses to increase ROS levels beyond the redox homeostasis threshold, which may cause serious side effects. How to disrupt redox homeostasis in cancer cells more effectively remains a challenge. In this study, we found that sodium selenite and docosahexaenoic acid (DHA), a polyunsaturated fatty acid extracted from marine fish, synergistically induced cytotoxic effects in colorectal cancer (CRC) cells. Physiological doses of DHA simultaneously upregulated oxidation and antioxidant levels within the threshold range without affecting cell viability. However, it rendered the cells more susceptible to reaching the upper limit of the threshold of redox homeostasis, facilitating the elevation of ROS levels beyond the threshold by combining with low doses of sodium selenite, thereby disrupting redox homeostasis and inducing MAPK-mediated paraptosis. This study highlights the synergistic anticancer effects of sodium selenite and DHA, which induce paraptosis by disrupting redox homeostasis in tumor cells. These findings offer a novel strategy for more targeted and less toxic cancer therapies for colorectal cancer treatment.
研究领域:
肿瘤学
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细胞生物学
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药物化学
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DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xinzhang Chen,Xu Lin
Trimethyl tin (TMT) is a good stabilizer for plastic products but is also a toxic environmental pollutant. Selenium has good antioxidant and anti-inflammatory properties and has been widely used in the poultry industry. However, it has not been reported whether selenium enrichment can antagonize TMT-induced viral hepatitis in poultry. To fill this gap, AA broiler models exposed to TMT for 42 d were established and fed a Se-enriched diet at the same time. H&E staining showed that selenium could significantly alleviate TMT-induced liver inflammation. Further analysis of the underlying mechanism revealed that TMT induced nitrosation stress (RNS), increased NO content and iNOS expression, which in turn activated the NF-κB/NLRP3 pathway and induced pyroptosis. However, selenium enrichment can reverse this situation, that is, reduce the occurrence of RNS, reduce the degree of pyroptosis, and thus alleviate the occurrence of inflammation. In our study, we demonstrated for the first time that TMT could induce hepatotoxicity other than neurotoxicity in poultry and that selenium could antagonize TMT-induced hepatotoxic hepatitis in chickens through the RNS/NF-κB/NLRP3 pathway.
研究领域:
毒理学
·
兽医学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Kexin Wang,Wenqi Zhao,Hongxuan Zhao,Jing Xu,Sisi Gao,Shuang-kui Du,Xiaoli Gao
Selenium (Se) fortification is essential for meeting nutritional requirements and preventing Se deficiency-related diseases. This study evaluated the effects of sodium selenite (Na 2 SeO 3 ) and selenomethionine (SeMet) on mung bean sprouts (MBS) by investigating Se accumulation, distribution, and protein physicochemical properties. The findings indicated that the Se accumulation efficiency of SeMet treatment was 3.22 times that of Na 2 SeO 3 treatment. Furthermore, both treatments achieved an organic Se conversion rate exceeding 95%. Distribution analysis revealed that 72.39% to 78.50% of organic Se was incorporated into proteins, while 6.28% to 6.93% was bound to polysaccharides. The order of organic Se accumulation in protein components was albumin > glutelin > globulin > prolamin. Additionally, Se enrichment enhanced Se accumulation in low molecular weight protein (25-33 kDa) and promoted protein synthesis, but decreased the cysteine and methionine content. These changes led to an increase in β-turn, disulfide bonds, and surface hydrophobicity, while reducing the accessibility of sulfhydryl and pigment groups. Consequently, the oil absorption capacity and foaming capacity of the protein were enhanced. The results provide a foundation for the utilization of Se-enriched MBS protein as a functional food ingredient.
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 71
·40984-40988
·
IF 2.936
Xiaonan Gao,Wenfei Guo,Lihong Ge,Fanpeng Kong,Kehua Xu,Bo Tang
Sodium selenite (Na2SeO3), as an anti-tumor drug for inducing tumor cells' apoptosis, has been widely studied under normoxic conditions and has been shown to exhibit oxidative stress process-induced apoptosis. However, since the real tumor environment is hypoxic, the actual mechanism is still unclear. Hence, considering the main metabolite of Na2SeO3 in the metabolic process to be hydrogen selenide (H2Se) and also that it can be converted to superoxide anion (O2˙−) instantaneously in the presence of oxygen, a dual-ratiometric fluorescence imaging system for simultaneous monitoring of the changes of H2Se and O2˙− induced by Na2SeO3-guided tumor cell apoptosis under hypoxic conditions was constructed. Two molecular probes NIR-H2Se and dihydroethidium were used to detect H2Se and O2˙−, respectively, whereas Rhodamine 110 was used as the reference fluorophore. Notably, H2Se levels significantly increased under hypoxic conditions, but there was no change in the level of O2˙−, which is inconsistent with the results of the previous researches. Therefore, we hypothesize that the mechanism of Na2SeO3-induced apoptosis for tumor cells is caused by reductive stress; also, this method can be applied for the future study of other anti-cancer selenium compounds.
研究领域:
生物化学
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医学影像学
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肿瘤学
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DOI
高影响因子
Science China-Life Sciences
·
2020
·Vol. 64
·期 3
·443-451
·
IF 4.611
Luan Dongrui,Zhao Zengteng,Xia Dandan,Zheng Qiuling,Gao Xiaonan,Xu Kehua,Tang Bo
Sodium selenite has alleviating effects on liver fibrosis; however, its therapeutic molecular mechanism remains unclear. Herein, hydrogen selenide, a major metabolite of Na 2 SeO 3 , was tested to uncouple the sulfilimine bond in collagen IV, the biomarker of liver fibrosis. A mouse model of liver fibrosis was constructed via a CCl 4 -induced method, followed by the administration of 0.2 mg kg −1 Na 2 SeO 3 via gavage three times per week for 4 weeks. Changes in H 2 Se, NADPH, and H 2 O 2 levels were monitored in real time by using NIR-H 2 Se, DCI-MQ-NADPH, and H 2 O 2 probes in vivo , respectively. H 2 Se continuously accumulated in the liver throughout the Na 2 SeO 3 treatment period, but the levels of NADPH and H 2 O 2 decreased. The expression of collagen IV was analyzed through Western blot and liquid chromatography-mass spectrometry. Results confirmed that the sulfilimine bond of collagen IV in the fibrotic mouse livers could be broken by H 2 Se with the Na 2 SeO 3 treatment. Therefore, the therapeutic effect of Na 2 SeO 3 on liver fibrosis could be mainly attributed to H 2 Se that uncoupled the sulfilimine bond to induce collagen IV degradation. This study provided a reasonable explanation for the molecular mechanism of the in vivo function of Na 2 SeO 3 and the prevention of liver fibrosis by administering inorganic selenium.
研究领域:
药理学
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生物化学
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肝病学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 7
·1418
·
IF 4.2
Xiaoli Li,Ying Li,Xueyan Wang,Rui Zhang,Jiaojiao Xue,Yi Ding,Xiuling Chu,Jianqing Su
Acanthopanax senticosuspolysaccharide–nano-selenium (ASPS-SENPS) andA. selenopanaxselenized polysaccharides (Se-ASPS) were synthesized, and their characterization and biological properties were compared. The acid extraction method was used to extract the polysaccharides ofA. selenopanax, followed by decolorization using the hydrogen peroxide method and deproteinization based on the Sevage method, and the purification ofA. senticosuspolysaccharides (ASPS) was carried out using the cellulose DEAE-52 ion column layer analysis method. AnA. senticosuspolysaccharide–nano-selenium complex was synthesized by a chemical reduction method using ASPS as dispersants. The selenization of polysaccharides fromA. selenopanaxwas carried out using the HNO3-Na2SeO3method. The chemical compositions, scanning electron microscopy images, infrared spectra, and antioxidant properties of ASPS-SENPS and Se-ASPS were studied, and they were also subjected to thermogravimetric analysis. The results indicated that the optimal conditions for the synthesis of ASPS-SENPS include the following: when ASPS accounts for 10%, the ratio of ascorbic acid and sodium selenium should be 4:1, the response time should be 4 h, and the reaction temperature should be 50 °C. The most favorable conditions for the synthesis of Se-ASPS were as follows: m (Na2SeO3):m (ASPS) = 4:5, response temperature = 50 °C, and response time = 11.0 h. In the in vitro antioxidant assay, when the mass concentration of Se-ASPS and ASPS-SENPS was 5 mg/mL, the removal rates for DPPH free radicals were 88.44 ± 2.83% and 98.89 ± 3.57%, respectively, and the removal rates for ABTS free radicals were 90.11 ± 3.43% and 98.99 ± 1.73%, respectively, stronger than those for ASPS. The current study compares the physiological and bioactivity effects of ASPS-SENPS and Se-ASPS, providing a basis for future studies on polysaccharides.
研究领域:
药学
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生物化学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 9
·1685
·
IF 7
Lingyu Xie,Yibin Xu,Xiaoqing Ding,Kaixuan Li,Shuang Liang,Danlei Li,Yongxia Wang,Aikun Fu,Weixiang Yu,Xiuan Zhan
Earlier studies have shown that selenomethionine (SM) supplements in broiler breeders had higher deposition in eggs, further reduced the mortality of chicken embryos, and exerted a stronger antioxidant ability in offspring than sodium selenite (SS). Since previous studies also confirmed that Se deposition in eggs was positively correlated with maternal supplementation, this study aimed to directly investigate the antioxidant activities and underlying mechanisms of SS and SM on the chicken hepatocellular carcinoma cell line (LMH). The cytotoxicity results showed that the safe concentration of SM was up to 1000 ng/mL, while SS was 100 ng/mL. In Se treatments, both SS and SM significantly elevated mRNA stability and the protein synthesis rate of glutathione peroxidase (GPx) and thioredoxin reductase (TrxR), two Se-containing antioxidant enzymes. Furthermore, SM exerted protective effects in the H2O2-induced oxidant stress model by reducing free radicals (including ROS, MDA, and NO) and elevating the activities of antioxidative enzymes, which performed better than SS. Furthermore, the results showed that cotreatment with SM significantly induced apoptosis induced by H2O2on elevating the content of Bcl-2 and decreasing caspase-3. Moreover, investigations of the mechanism revealed that SM might exert antioxidant effects on H2O2-induced LMHs by activating the Nrf2 pathway and enhancing the activities of major antioxidant selenoenzymes downstream. These findings provide evidence for the effectiveness of SM on ameliorating H2O2-induced oxidative impairment and suggest SM has the potential to be used in the prevention or adjuvant treatment of oxidative-related impairment in poultry feeds.Keywords:selenomethionine;sodium selenite;antioxidants;glutathione peroxidase;Nrf2
研究领域:
动物科学
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生物化学
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细胞生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 3
·172
·
IF 6.085
Jianping Chen,Xuehua Chen,Jiarui Li,Baozhen Luo,Tugui Fan,Rui Li,Xiaofei Liu,Bingbing Song,Xuejing Jia,Saiyi Zhong
In the present study, a selenium-chondroitin sulfate (SeCS) was synthesized by the sodium selenite (Na2SeO3) and ascorbic acid (Vc) redox reaction using chondroitin sulfate derived from shark cartilage as a template, and characterized by SEM, SEM-EDS, FTIR and XRD. Meanwhile, its stability was investigated at different conditions of pH and temperatures. Besides, its antioxidant activity was further determined by the DPPH and ABTS assays. The results showed the SeCS with the smallest particle size of 131.3 ± 4.4 nm and selenium content of 33.18% was obtained under the optimal condition (CS concentration of 0.1 mg/mL, mass ratio of Na2SeO3to Vc of 1:8, the reaction time of 3 h, and the reaction temperature of 25 °C). SEM image showed the SeCS was an individual and spherical nanostructure and its structure was evidenced by FTIR and XRD. Meanwhile, SeCS remained stable at an alkaline pH and possessed good storage stability at 4 °C for 28 days. The results on scavenging free radical levels showed that SeCS exhibited significantly higher antioxidant activity than SeNPs and CS, indicating that SeCS had a potential antioxidant effect.Keywords:nanoselenium;chondroitin sulfate derived from shark cartilage;structure characterization;antioxidant activity
研究领域:
纳米技术
·
材料科学
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生物化学
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药学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2020
·
IF 3.365
Chen Xujun,Li Shuyi,Cong Xin,Yu Tian,Zhu Zhenzhou,Barba Francisco J.,Marszalek Krystian,Puchalski Czesław,Cheng Shuiyuan
Selenium is an essential trace element and micronutrient for human health. Application of organic selenium in plants and microorganisms as trace element supplement is attracting more and more attention. In this study, Bacillus cereus, an important probiotic, was used for selenium enrichment with sodium selenite as selenium source. The growth curve of B. cereus was investigated, and 150 μg/ml was selected as the concentration of selenium for B. cereus fermentation. With application of response surface methodology, the optimal fermentation conditions were obtained as follows: inoculation quantity of 7%, culture temperature of 33°C, and shaking speed of 170 rpm, leading to the maximal selenium conversion ratio of 94.3 ± 0.2%. Field emission scanning electron microscope and energy dispersive spectrometry evidenced that inorganic selenium had been successfully transformed. This study may contribute to get a strain with high Se conversion ratio, so as to extract organic selenium in the form of selenoprotein to be used for further application.
研究领域:
微生物学
·
营养科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Furong Wang,Min Yan,Ting Zhang,Zhen Tian,Xinxin Niu,Zimeng Guo,Xian Wang,Xiaoben Li,Wei Song,Yan'e Luo,Yahong Yuan,Tianli Yue
Conversion of inorganic selenium (Se) to its organic form by Saccharomyces cerevisiae ( S. cerevisiae ) has attracted significant attention, for organic Se has higher nutritional value with less negative effects. However, the mechanism of transformation and accumulation of organic Se at the metabolic level remains unclear. We investigated the metabolic response to sodium selenite supplementation in culture medium at 0, 5, 15, and 45 mg/L in two S. cerevisiae strains using an ultra-high performance liquid chromatography-high resolution mass spectrometry-based untargeted method. In total, 455 metabolites were detected, and a separation trend was clearly observed between Se-treated groups and controls. In depth, metabolic pathways involving amino acid, nucleotide metabolism and tricarboxylic acid cycle were significantly affected. Furthermore, high inorganic-Se supplementation effectively decreased the seleno-amino acid proportion in total Se, inhibited cell growth, and decreased glutathione levels. These results provide novel insights into the Se-enrichment process of S. cerevisiae .
研究领域:
微生物学
·
代谢组学
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营养学
·
生物化学
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产品关联
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Zheng Lin,Zhu Hua-Zhang,Wang Bing-Tao,Zhao Qiong-Hui,Du Xiu-Bo,Zheng Yi,Jiang Liang,Ni Jia-Zuan,Zhang Yan,Liu Qiong
Many studies have shown that imbalance of mineral metabolism may play an important role in Alzheimer’s disease (AD) progression. It was recently reported that selenium could reverse memory deficits in AD mouse model. We carried out multi-time-point ionome analysis to investigate the interactions among 15 elements in the brain by using a triple-transgenic mouse model of AD with/without high-dose sodium selenate supplementation. Except selenium, the majority of significantly changed elements showed a reduced level after 6-month selenate supplementation, especially iron whose levels were completely reversed to normal state at almost all examined time points. We then built the elemental correlation network for each time point. Significant and specific elemental correlations and correlation changes were identified, implying a highly complex and dynamic crosstalk between selenium and other elements during long-term supplementation with selenate. Finally, we measured the activities of two important anti-oxidative selenoenzymes, glutathione peroxidase and thioredoxin reductase, and found that they were remarkably increased in the cerebrum of selenate-treated mice, suggesting that selenoenzyme-mediated protection against oxidative stress might also be involved in the therapeutic effect of selenate in AD. Overall, this study should contribute to our understanding of the mechanism related to the potential use of selenate in AD treatment.
研究领域:
神经科学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Menghan Yuan,Xinyue Huang,Shuang Tian,Shixuan Ma,Yuxing Guo,Mingxuan Tao
The selenized modification of purified Pleurotus eryngii polysaccharides (PPEP) were carried out in a nitric acid-sodium selenite system, and the preliminary structural characterization of PPEP and selenized PPEP (613.87 μg/g of selenium) (SPPEP) were carried out by advanced instrumentation techniques. It was found that the selenization modification did not seriously affect the structure of the PPEP. However, the SPPEP exhibited a lower molecular weight, more glycosidic bonds, reduced crystallinity, and improved thermal stability. Through in vivo animal experiments, the study found that PPEP and SPPEP were non-toxic to macrophages. Furthermore, they markedly increased the thymus and spleen indices of immunocompromised mice and improved the histomorphology of these organs. Moreover, PPEP and SPPEP enhanced the mice’s immune ability by markedly increasing the level of immune factor secretion, bone marrow hematopoietic function, and macrophage phagocytic capacity. In addition, the immune activity of SPPEP was better than PPEP’s activity.
研究领域:
生物技术
·
免疫学
·
药学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）