ENVIRONMENTAL RESEARCH
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2025
·
IF 7.7
Zi-Shang Ma,Han-Min Zhang,Wen-Jie Ma,Nuo-Yan Liu,Xue-Cheng Yu
Traditional heterotrophic denitrification encounters challenges in the treatment of wastewater with a low carbon to nitrogen ratio (C/N). This study successfully achieved complete nitrogen removal with energy efficiency through the anaerobic-anoxic-anoxic sulfur autotrophic (A 3 ) process in the treatment of low C/N wastewater. Notably, this process enabled simultaneous nitrification-heterotrophic denitrification (SND) coupled with sulfur-driven autotrophic denitrification (SAD) with a nitrogen removal rate of 0.15 kg N/m 3 /d (100 % of NH 4 + -N and 92 % of total nitrogen (TN) removal). More than 85 % of TN removal and 50 % of SND contribution were obtained under low-temperature conditions (about 10–14 °C). After cultivation in the A 3 system, the dominant bacterial genus changed from norank_f__Saprospiraceae (11.22 %–5.21 %) to Ferruginibacter (0.89 %–19.31 %). Additionally, Nitrospira (1.34 %) and Thiobacillus (2.14 %) exhibited a synergistic effect in the anoxic tank, collectively promoting the simultaneous nitrification and denitrification process. This study proposes a novel and energy-efficient approach for the treatment of low C/N wastewater.
研究领域:
环境工程
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废水处理
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微生物生态学
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生物脱氮
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DOI
PubMed
高影响因子
Processes
·
2019
·Vol. 7
·期 11
·827
·
IF 1.963
Yong Li,Siyuan Zhao,Jiejie Zhang,Yang He,Jianqiang Zhang,Rong Ge
The aerobic denitrifying phosphate accumulating bacteria (ADPB) use NO3−as an electron acceptor and remove nitrate by denitrification and concomitant uptake of excessive phosphorus in aerobic conditions. Activated sludge was collected from the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town, Chengdu City. The candidate ADPB strains were obtained by cultivation in the enriched denitrification media, followed by repeated isolation and purification on bromothymol blue (BTB) solid plates. The obtained candidates were further screened for ADPB strains by phosphorus uptake experiment, nitrate reduction test, metachromatic granules staining, and poly-β-hydroxybutyrate (PHB) staining. The 16 sedimentation ribosome deoxyribonucleic acid (16 S rDNA) molecular technique was used to determine their taxonomy. Further, the denitrification and dephosphorization capacities of ADPB strains were ascertained through their growth characteristics in nitrogen-phosphorus-rich liquid media. The results revealed a total of 25 ADPB strains screened from the activated sludge of the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town. These strains belonged to two classes, four orders, and five genera. Among them, the strain SW18NP2 was a potentially new species in theAcinetobactergenus, while the strain SW18NP24 was a potential new species in thePseudomonasgenus at the time of their characterization. TheAcinetobacterwas the dominant genus. The obtained ADPB strains demonstrated a rich diversity. The ADPB strains had significant variations in denitrification and dephosphorization capacities. Twenty-three strains exhibited a total phosphorus removal rate of above 50%, and 19 strains exhibited a total nitrogen removal rate of above 50%. The strain SW18NP2 showed the best denitrifying phosphorus removal (DPR) capacity, with a dephosphorization rate of 82.32% and a denitrification rate of 73.73%. The ADPB in the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town demonstrated a rich diversity and a strong DPR capacity.Keywords:aerobic denitrifying phosphate accumulating bacteria (ADPB);diversity;denitrifying phosphorus removal
研究领域:
环境微生物学
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污水处理
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DOI
高影响因子
Defence Technology
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2021
·
IF 3.172
Ling Liao,Bo Jin,Zhi-cheng Guo,Fei Xian,Chen-jie Hou,Ru-fang Peng
To explore the effect of different positions and number of pyrrolidine bound to the carbon cage on the stabilization effect of fulleropyrrolidine derivatives to nitrocellulose (NC)/nitroglycerine (NG), we synthesized N -(4-methoxy) phenylpyrrolidine-C 60 and four different of bis( N -(4-methoxy) phenylpyrrolidine)-C 60 compounds through Prato reaction. Their structures were characterized by UV–vis, 1 H NMR, 13 C NMR, high-resolution mass spectroscopy, and single-crystal X-ray diffraction. Their stabilization effect to NC/NG were investigated using differential scanning calorimetry, methyl violet, vacuum stabilization effect, weight loss, and accelerating rate calorimeter tests. The results indicated these compounds had excellent stabilization effect to NC/NG. The stabilization effect of the fulleropyrrolidine bisadducts to NC/NG is significantly better than that of fulleropyrrolidine monoadduct and C 60 . Moreover, the position where pyrrolidine binds to fullerene in fulleropyrrolidine bisadducts is different, and its stabilization effect to NC is also different. The stabilization effect order of different bisadduct isomers to nitrocellulose is as follows: e-edge > trans-2> cis-2> trans-3. Electron paramagnetic resonance (EPR) and FT-IR were used to study the stabilization mechanism of fulleropyrrolidine derivatives to NC/NG. The EPR results also show that fulleropyrrolidine bisadducts with different addition sites have different abilities to absorb nitroxide, and their ability is better than that of the monoadduct and C 60 , which is consistent with the results of stabilization effect performance test.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
Frontiers of Environmental Science & Engineering
·
2021
·Vol. 16
·期 6
·1-10
·
IF 4.357
Man Quanli,Zhang Peilian,Huang Weiqi,Zhu Qing,He Xiaoling,Wei Dongsheng
A novel salt-tolerant heterotrophic nitrification and aerobic denitrification (HN-AD) bacterium was isolated and identified as Halomonas venusta TJPU05 ( H. venusta TJPU05). The nitrogen removal performance of H. venusta TJPU05 in simulated water (SW) with sole or mixed nitrogen sources and in actual wastewater (AW) with high concentration of salt and nitrogen was investigated. The results showed that 86.12% of NH 4 + -N, 95.68% of NO 3 − -N, 100% of NO 2 − -N and 84.57% of total nitrogen (TN) could be removed from SW with sole nitrogen sources within 24 h at the utmost. H. venusta TJPU05 could maximally remove 84.06% of NH 4 + -N, 92.33% of NO 3 − -N, 92.9% of NO 2 − -N and 77.73% of TN from SW with mixed nitrogen source when the salinity was above 8%. The application of H. venusta TJPU05 in treating AW with high salt and high ammonia nitrogen led to removal efficiencies of 50.96%, 47.28% and 43.19% for NH 4 + -N, NO 3 − -N and TN respectively without any optimization. Furthermore, the activities of nitrogen removal-related enzymes of the strain were also investigated. The successful detection of high level activities of ammonia oxygenase (AMO), hydroxylamine oxidase (HAO), nitrate reductase (NAR) and nitrite reductase (NIR) enzymes under high salinity condition further proved the HN-AD and salt-tolerance capacity of H. venusta TJPU05. These results demonstrated that the H. venusta TJPU05 has great potential in treating high-salinity nitrogenous wastewater.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2024
·
IF 2.3
Mengyao Gui
Humic acid (HA), a common natural organic matter , could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in Achromobacter sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L −1 ) promoted the aerobic denitrification process (excluding N 2 O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L −1 h −1 ) and lower levels of nitrite accumulation (30.2–20.7 mg L −1 ). This was attributed to the increased electron transfer activities and denitrifying reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as napA , cnorB , and nirS was enhanced by HA. Nonetheless, the nosZ gene and N 2 OR activity underwent suppression by HA, which was accountable for N 2 O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers for wastewater treatment plants to enhance nitrogen removal.
研究领域:
环境生物学
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废水处理
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Juanjuan Yao,Huiying Zhang,Longfu Chen,Wei Liu,Naiyun Gao,Shiyi Liu,Xiangyu Chen,Fanghui Rao
The potential risks of sono-induced nitrosation and nitration side reactions and consequent toxic nitrogenous byproducts were first investigated via sono-degradation of diphenylamine (DPhA) in this study. The kinetic models for overall DPhA degradation and the formation of nitrosation byproduct (N-nitrosodiphenylamine, NDPhA) and nitration byproducts (2-nitro-DPhA and 4-nitro-DPhA) were well established and fitted (R 2 > 0.98). Nitrosation contributed much more than nitration (namely, 43.3 – 47.3 times) to the sono-degradation of DPhA. The contribution of sono-induced nitrosation ranged from 0.4 to 56.6% at different conditions. The maximum NDPhA formation rate and the contribution of sono-induced nitrosation were obtained at 600 and 200 kHz, respectively, as ultrasonic frequencies at 200 to 800 kHz. Both NDPhA formation rate and the contribution of sono-induced nitrosation increased with increasing power density, while decreased with increasing initial pH and DPhA concentration. PO 4 3– , HCO 3 – , NH 4 + and Fe 2+ presented negative impacts on sono-induced nitrosation in order of HCO 3 – >> Fe 2+ > PO 4 3– > NH 4 + , while Br – exhibited a promoting effect. The mechanism of NDPhA formation via sono-induced nitrosation was first proposed.
研究领域:
环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
GLOBAL CHANGE BIOLOGY
·
2021
·Vol. 27
·期 21
·5564-5579
·
IF 10.863
Xiaoxuan Su,Teng Wen,Yingmu Wang,Junshi Xu,Li Cui,Jinbo Zhang,Ximei Xue,Kai Ding,Yijia Tang,Yong-guan Zhu
Ocean acidification in nitrogen-enriched estuaries has raised global concerns. For decades, biotic and abiotic denitrification in estuarine sediments has been regarded as the major ways to remove reactive nitrogen, but they occur at the expense of releasing greenhouse gas nitrous oxide (N 2 O). However, how these pathways respond to acidification remains poorly understood. Here we performed a N 2 O isotopocules analysis coupled with respiration inhibition and molecular approaches to investigate the impacts of acidification on bacterial, fungal, and chemo-denitrification, as well as N 2 O emission, in estuarine sediments through a series of anoxic incubations. Results showed that acidification stimulated N 2 O release from sediments, which was mainly mediated by the activity of bacterial denitrifiers, whereas in neutral environments, N 2 O production was dominated by fungi. We also found that the contribution of chemo-denitrification to N 2 O production cannot be ignored, but was not significantly affected by acidification. The mechanistic investigation further demonstrated that acidification changed the keystone taxa of sedimentary denitrifiers from N 2 O-reducing to N 2 O-producing ones and reduced microbial electron-transfer efficiency during denitrification. These findings provide novel insights into how acidification stimulates N 2 O emission and modulates its pathways in estuarine sediments, and how it may contribute to the acceleration of global climate change in the Anthropocene.
研究领域:
海洋生物学
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环境科学
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微生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2023
·
IF 11.4
Mingxing Hu,Hanmin Zhang,Yu Tian
An innovative partial nitrification and short-cut sulfur autotrophic denitrification (PN-SSAD, NH 4 + -N → NO 2 − -N → N 2 ) coupled system in a single-stage SBR was proposed to treat low C/N wastewater with low material and energy consumption. Nearly 50 % alkalinity consumption and 40 % sulfate production were reduced in S 0 -SSAD compared with S 0 -SAD, whereas the autotrophic denitrification rate was increased by 65 %. In S 0 -PN-SSAD, the TN removal efficiency reached almost 99 % without additional organic carbon. Furthermore, pyrite (FeS 2 ) rather than S 0 served as the electron donor to optimize the PN-SSAD process. The practical sulfate production in S 0 -PN-SSAD and FeS 2 -PN-SSAD were about 38 % and 52 % lower than complete nitrification and sulfur autotrophic denitrification (CN-SAD), respectively. Thiobacillus was the major autotrophic denitrification bacteria in S 0 -PN-SSAD (34.47 %) and FeS 2 -PN-SSAD (14.88 %). Nitrosomonas and Thiobacillus played a synergistic effect in the coupled system. FeS 2 -PN-SSAD is expected as an alternative technology for nitrification and heterotrophic denitrification (HD) in treating low C/N wastewater.
研究领域:
环境工程
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微生物学
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水处理技术
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2020
·Vol. 11
·期 1
·58
·
IF 4.324
Fayang Guan,Hui Ren,Lan Yu,Qingzhong Cui,Wanjun Zhao,Jie Liu
In order to synthesize a novel two-dimensional energetic material, nitrated graphene oxide (NGO) was prepared by the nitrification of graphite oxide to make a functional modification. Based on the morphological characterization, the NGO has a greater degree of curl and more wrinkles on the surface. The structure characterization and density functional theory calculation prove that epoxy and hydroxyl groups on the edge of graphite oxide have reacted with nitronium cation (NO2+) to produce nitro and nitrate groups. Hydrophobicity of NGO implied higher stability in storage than graphene oxide. Synchronous simultaneous analysis was used to explore the decomposition mechanism of NGO preliminarily. The decomposition enthalpy of NGO is 662.0 J·g−1and the activation energy is 166.5 kJ·mol−1. The thermal stability is similar to that of general nitrate energetic materials. The hygroscopicity, thermal stability and flammability of NGO prove that it is a novel two-dimensional material with potential applications as energetic additives in the catalyst, electrode materials and energetic devices.Keywords:nitrated graphene oxide;nitrification process;properties analysis;synchronous simultaneous analysis;combustion performance
研究领域:
材料科学
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化学工程
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能源科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Jianxin Pan,Liangliang Liu,Hanping Pan,Lihui Yang,Meirong Su,Chaohai Wei
Metal sulfide-based biological process is considered as a promising biotechnology for next-generation wastewater treatment . However, it is not clear if simultaneous bio-reduction of nitrate and chromate was achievable in this process. This study aimed to evaluate the feasibility of metal sulfides (FeS and MnS) on simultaneous denitrification and chromate reduction in autotrophic denitrifying column bioreactors . Results showed that simultaneous reduction of nitrate and chromate was achieved using metal sulfides (FeS and MnS) as electron donors, in which sulfate was the sole soluble end-product. Apart from the sulfur element in the metal sulfides, Fe(II) and Mn(II) were also involved in nitrate and chromate reduction as indicative by the formation of their oxidative states compounds. In microbial communities , SHD-231 and Thiobacillus were the most predominant bacteria, which might have played important roles in simultaneous denitrification and chromate reduction. Compared to FeS, MnS showed a higher performance on nitrate and chromate removal, which could also reduce the toxic inhibition of chromate on nitrate reduction . According to results of XRD and XPS, as well as a lower sulfate production in the FeS system, FeS might have been covered easily to hydroxides due to its bio-oxidation, which limited mass transfer efficiency and bio-availability of FeS. The findings in this study offered insights in the development of promising approaches for the treatment of toxic and hazardous compounds using metal sulfide.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
Water
·
2024
·Vol. 16
·期 3
·416
·
IF 3
Qiang Li,Yuehui He,Boyan Wang,Nanhai Weng,Lei Zhang,Kaichun Wang,Fengrong Tian,Mingsheng Lyu,Shujun Wang
The biological denitrification of low-C/N wastewater is a great challenge in treatment plants due to the lack of microorganisms with heterotrophic nitrification–aerobic denitrification (HN-AD) abilities. In this study,Bacillussp. L2 was isolated from aeration tank water samples using a nitrification medium and screened for its ability to perform HN-AD in low-C/N wastewater. The strain showed a maximum NH4+-N removal rate of 98.37% under low-C/N conditions. In the presence of a mixed N source, strain L2 was capable of completely removing NH4+-N within 24 h. Furthermore, optimal nitrogen removal conditions for strain L2 were found to be C/N = 9, pH = 9, and sodium acetate as the C source. Under optimal conditions, the strain was able to maintain a high NH4+-N removal rate under 0–3% salinity and an NH4+-N concentration of 200 mg/L or less. The denitrification pathways of strain L2 were NH4+→NH2OH→NO2−(↔NO3−)→NO→N2O→N2and NH4+→NH2OH→NO→N2O→N2. Furthermore, semi-continuous wastewater treatment was conducted using immobilized technology, which resulted in more than 82% NH4+-N removal after three cycles of reuse. This study demonstrates the great potential ofBacillussp. L2 in wastewater treatment applications.
研究领域:
环境微生物学
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污水处理
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产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Ze-Tong Feng,Yan Yu,Ying-Jun Sun,Jia-Min Zhou,Xin Ma,Fei Ding,Yi-Liu Zheng,Ren-Cun Jin,Chun-Qiang Shen,Qian-Qian Zhang
This study systematically evaluated the effects of intermittent versus continuous addition of zero-valent iron (ZVI) as exogenous enhancers on nitrogen removal performance in denitrification systems. Results shown that under carbon-to‑nitrogen ratios (C/N) was 3, 2, and 1, continuous addition of ZVI significantly improved total nitrogen removal efficiency (TNRE) by 8.57 %, 19.34 %, and 11.45 %, respectively, compared to the control system. Concurrently, extracellular polymeric substance (EPS) production increased by 32.43 %, 62.91 %, and 35.93 %, to that of intermittent ZVI addition system at the same phases. The continuous ZVI-added system enriched more mixotrophic denitrification bacteria and increased soluble iron ion concentrations through EPS-mediated electron shuttling and endogenous protection mechanisms. While, under C/N was 0, the TNRE in continuously ZVI-added system reduced to 8.3 ± 3.24 % due to iron mineral crust formation. Specifically, the TNRE of system with intermittent addition of ZVI stabilized at 17.03 ± 3.24 %, enriched more autotrophic denitrification bacteria and avoided excessive accumulation of iron oxides. Therefore, this experiment provided new insights into the stable operation of the ZVI-enhanced denitrification process for treating low C/N wastewater.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）