SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Hualai Wang,Fubin Pei,Chun Liu,Yue Ni,Mingzhu Xia,Shasha Feng,Qingli Hao,Tinghai Yang,Wu Lei
In the paper, a novel fluorescent probe based on Ag 2 S QDs/g-C 3 N 4 composite was synthesized by loading Ag 2 S quantum dots (Ag 2 S QDs) on the surface of g-C 3 N 4 through in-situ synthesis method and developed to detect Nitrofurazone (NFZ) sensitively. The results showed that the linear detection range of Ag 2 S QDs/g-C 3 N 4 to NFZ was 0–30 μM, with a low detection limit of 0.054 μM. The results of time-fluorescence-resolved spectroscopy and UV–vis absorption spectroscopy exhibited that the possible detection mechanism of Ag 2 S QDs/g-C 3 N 4 to NFZ was proposed to be Internal Filtration Effect (IFE). Moreover, Multiwfn wavefunction analysis was employed to uncover the possible interaction between the Ag 2 S QDs/g-C 3 N 4 and NFZ, thereby further revealing the fluorescence detection mechanism from the scale of atoms. Combining experiments and theoretical calculations, we proposed the sensing mechanism of the formation of non-fluorescent ground state complex linked by hydrogen bonds. This work indicated that the Ag 2 S QDs/g-C 3 N 4 composite processed the ability to detect NFZ efficiently and sensitively.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·5011-5017
·
IF 5.227
Jiaxi Yin,Hairong Cui,Ling Lei,Kangbing Wu
Graphene nanosheets (GS) were prepared by ultrasonic exfoliation of bulk graphite in N-methyl-2-pyrrolidone with the assistance of sodium pyrophosphate. The obtained GS suspension was modified on a glassy carbon electrode (GS/GCE), and then functionalized at different voltages (e.g. 1.0, 1.4 and 1.6 V) for 2 min in pH 7.0 phosphate buffer. The electrochemically functionalized GS/GCE (i.e. EGS/GCE) possesses more oxygen-containing groups and a higher defect level. More importantly, the active response area, electron transfer ability and interface adsorption capacity of the EGS/GCE enhanced remarkably. The possible mechanism of the performance enhancement is discussed, and the sensing application of the EGS/GCE in the detection of nitrofurazone (NFZ) is investigated. Compared with the GS/GCE, the EGS/GCE is much more active for NFZ oxidation and greatly increases the detection sensitivity. As a result, a highly sensitive electrochemical detection method has been developed for NFZ, with a detection limit of 2.1 nM. The practical application of the EGS/GCE was tested in fish meat samples, showing good accuracy and feasibility.
研究领域:
分析化学
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材料科学
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环境科学
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PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2019
·
IF 2.29
Yuxin Bao,Ali Farajtabar,Min Zheng,Hongkun Zhao
Solubilities of 5-nitrofurazone (form γ ) in aqueous co-solvents mixtures of isopropanol, N -methyl pyrrolidone (NMP), ethanol and dimethyl sulfoxide (DMSO) were experimentally investigated via the saturation shake-flask technique at temperatures from 278.15 K to 323.15 K under ambient pressure of p = 101.2 kPa. The solubility of 5-nitrofurazone increased to a maximum value with 100% mass fractions of NMP and DMSO in aqueous solutions of NMP and DMSO, while with 80% mass fractions of isopropanol and ethanol in aqueous solutions of isopropanol and ethanol. The solids equilibrated with liquor were characterized by X-ray power diffraction, indicating that no solvate formation or polymorphic transformation occurred. The Jouyban-Acree model was accepted to correlate the obtained solubility. The acquired highest relative average deviation and root-mean-square deviation values were, respectively, 5.62 × 10 −2 and 11.18 × 10 −4 . Quantitative values for local mole fractions of NMP (ethanol, DMSO, DMF or isopropanol) and water nearby 5-nitrofurazone were obtained by the method of Inverse Kirkwood–Buff integrals. The parameters of preferential solvation were positive for isopropanol/ethanol in the isopropanol/ethanol mixtures in intermediate compositions, and for NMP/DMF/DMSO in the NMP/DMF/DMSO mixtures in intermediate and NMP/DMF/DMSO-rich compositions, which indicated that 5-nitrofurazone was preferentially solvated by the co-solvents. Linear solvation energy relationships were used to model the solvent effect through the analysis of correlations between solubility data and solvent polarity quantified by suitable descriptors.
研究领域:
药学
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化学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xu Wen,Xueling Yan,Xiaoling Zheng,Qiming Kou,Lulan Yang,Jiaming Tang,Xingyue Chen,Yong Xie,Tao Le
Nitrofurazone (NFZ) is an antibiotic banned in many countries, as its residue seriously harms the human body. Herein, anti-NFZ aptamers were selected and identified based on the magnetic bead SELEX technique using a ssDNA library with a full length of 90 nucleotides (nt). Five full sequence candidate aptamers (NFZ8, NFZ24, NFZ28, NFZ34, and NFZ70) were obtained by secondary structure analysis. We optimized the entire sequence to obtain a truncated aptamer, a 16 nt sequence (NFZ8-1:5′-GTTCTATTGAAAAAAC-3′) that showed the highest affinity for NFZ (K d = 76.11 nM). The binding site of NFZ and aptamer NFZ8-1 was found to be "GAA" by molecular docking . In addition, utilizing the most special truncated aptamer NFZ8-1 as the identification probe, a graphene oxide fluorescent aptasensor is an innovative for the detection of NFZ residue that showed a wide linear reach from 1.25 to 160 ng/mL and a low limit of detection of 1.13 ng/mL. In the actual water environment sample detection, the recovery rate ranged from 95.21 to 113.66%, and the coefficient of variation ranged from 3.53 to 11.24%. These results demonstrate that the NFZ-truncated aptamer applied to the aptasensor provides a novel methodology for recognizing NFZ residues.
研究领域:
生物化学
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分析化学
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药物检测
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuyun Bi,Di Shao,Yue Yuan,Rui Zhao,Xu Li
A novel surface-enhanced Raman spectroscopy (SERS) method for the determination of nitrofurazone was developed using AuNPs/ γ -Al 2 O 3 nanoparticles protected by β -cyclodextrin ( β -CD) as substrate prepared in our lab. The optimum experimental conditions were obtained from single factor procedure and response surface modeling. A linear relationship ( I SERS = 508.96 c + 31987.87, c : nmol L −1 , R 2 = 0.996) between SERS intensity and the concentration of nitrofurazone in the range of 3.3 – 667.0 nmol L −1 was established, the limit of detection (LOD) was found at nmol L −1 level (0.37 nmol L −1 by 3 S 0 / S ). The selectivity for the method was studied by the influences of foreign substances on the determination. The recoveries and RSD (n = 5) for the six meat samples were 95.1 % – 104.5% and 2.4 % – 4.8% respectively, which suggesting that the new SERS method was successfully to detecting nitrofurazone.
研究领域:
分析化学
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食品安全检测
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Wu Huizhen,Liu Zhi,Zhang Hu,Xie Shuangnan,Tang Chao,Liu Wei,Li Bin,Chen Piao,Zhang Zihong,Qian Mingrong
Endogenous SEM (SEM end ) naturally occurs in crustacea aquatic products, it confuses the legal verdict of exogenous SEM (SEM ex ) degraded by antibiotic nitrofurazone abuse. In the present study, compound-specific gas phase chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) analysis coupled with a two-step pre-column derivatization was developed for determining the nitrogen stable ratio (δ 15 N) of SEM in shrimp and crab. The optimal derivatization and solid phase extraction (SPE) condition of SEM were screened by Plackett-Burman design and central composite design (CCD) experiment. The nitrogen isotope ratio analysis (NSIRA) of SEM in two types of crustaceans proved that its δ 15 N values significantly decreased from 7.0‰ to 2.0‰ once nitrofurazone being used. Analytical results found that the δ 15 N value of SEM ex in vivo was consistent its nitrofurazone precursor but the value of SEM end was dependent on N-containing components (e.g., tissue protein), ANOVA showed significant difference between two types of SEM (p < 0.05). Based on the δ 15 N variations between SEM itself and its nitrofurazone precursor, tissue protein of crustaceans, the percentages of SEM ex and SEM end can be calculated for its source apportionment in vivo. The limit of detection of SEM is down to 0.21 μg kg −1 and 0.54 μg kg −1 , and the identification lower limit of SEM ex are accurate at 6.27% and 0% for two types of crustaceans. The strategy first realized the independent determination of SEM ex and SEM end in crustacea aquatic products, which explained that high-level SEM in crustaceans can’t as the legal verdict of nitrofurazone abuse in aquaculture.
研究领域:
食品安全
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分析化学
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生物学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yuting Wang,Fengming Zhang,Jia Liu,Bin Yang,Yue Yuan,Yanyan Zhou,Shuyun Bi
A method was established for detecting the content of nitrofurazone (NFZ) by fluorescence quenching of N-Acetyl-L-Cysteine (NAC) coated cadmium telluride quantum dots (CdTe QDs). By means of transmission electron microscopy (TEM) and multispectral methods such as fluorescence and ultraviolet visible spectra (UV–vis), the synthesized CdTe QDs were characterized. The quantum yield ( φ ) of CdTe QDs was measured as 0.33 by reference method. The CdTe QDs had a better stability, the RSD of fluorescence intensity was 1.51% in three months. NFZ quenching the emission light of CdTe QDs was observed. The analyses of Stern-Volmer and time-resolved fluorescence suggested the quenching was static. The binding constants ( K a ) between NFZ and CdTe QDs were 1.14 × 10 4 (293 K), 0.74 × 10 4 (303 K) and 0.51 × 10 4 (313 K) L mol −1 . The hydrogen bond or van der Waals force was the dominated binding force between NFZ and CdTe QDs. The interaction was further characterized by UV–vis absorption as well as Fourier transform infrared spectra (FT-IR). Using fluorescence quenching effect, a quantitative determination of NFZ was carried out. The optimal experimental conditions were studied and determined as following: pH was 7 and contact time was 10 min. The effects of reagent addition sequence, temperature and the foreign substances including some metals (Mg 2+ ; Zn 2+ ; Ca 2+ ; K + ; Cu 2+ ), glucose, bovine serum albumin (BSA) and furazolidone on the determination were studied. There was a high correlation between the concentration of NFZ (0.40 – 39.63 μg mL −1 ) and F 0 / F with the standard curve F 0 / F = 0.0262 c + 0.9910 ( r = 0.9994). The detection limit (LOD) reached 0.04 μg mL −1 (3 S 0 / S ). The contents of NFZ in beef and bacteriostatic liquid were detected. The recovery of NFZ was 95.13% – 103.03% and RSD was 0.66% – 1.37% ( n = 5).
研究领域:
分析化学
·
生物医学工程
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PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·H425
·
IF 3.12
Chunxiang Chen,Wen Chen,Jinlong Jiang,Lei Qian,Xue Zhang
In this study, nitrofurazone (NF), a commonly used but banned drug in aquaculture, was used as the research model for nitrofuran antibiotics. A poly-alizarin red modified glassy carbon electrode (PAR/GCE) was prepared by electropolymerization. On this modified electrode, NF can produce a sensitive and stable reduction peak. Other nitrofuran antibiotics, such as nitrofurantoin and furazolidone, showed similar responses. The reduction peak of NF was proportional to the concentration within the range of 3.0–50.0 and 50.0–200.0 μM, and the detection limit was 0.33 μM. The linear ranges of furantoin and furazolidone were 10–40 and 50–140 μM, respectively, with corresponding detection limits of 0.73 and 1.56 μM. The modified electrode was used to detect NF in the cultured water sample, and the recovery rate of the sample was 94.5%–104.8%. Thus, the result was satisfactory.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Huan Lu,Minmin Liu,Hanyue Cui,Yan Huang,Li Li,Yaping Ding
The combination of molecularly imprinted technology and electrochemical sensor can prepare stable and excellent anti-interference molecularly imprinted electrochemical sensor (MIECS), realizing the highly specific recognition of nitrofurazone (NFZ). Bifunctional monomers and zeolitic imidazolate frameworks (ZIF) modified carboxylic multi-walled carbon nanotubes (c-MWCNTs) nanocompostites were used to solve the problems of low conductivity and limited number of imprinted holes in molecularly imprinted polymer (MIP) membrane. The porous nanocomposite can efficiently improve the conductivity and increase the surface of electrode, providing abundant molecularly imprinted holes and enabling a wide detection range. Nicotinamide (NA) and 2-amino-5-mercapto-1,3,4-thiadiazole (AMT) acted as bifunctional monomers and NFZ as template molecules, which were electropolymerized to MIP on the modified electrode . In the optimal experimental environment, the electrochemical response of MIECS to NFZ was linear in a certain concentration range. The linear range was from 10 –13 M to 10 –6 M, and the detection limit was 6.7 ×10 –14 M. The MIECS showed excellent anti-interference performance, which was attributed to the unique holes generated by the polymerization of bifunctional monomers. MIECS also owned good stability and reproducibility and has been successfully applied to the detection of actual urine and water samples.
研究领域:
分析化学
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生物传感
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环境科学
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DOI
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2016
·
IF 1.878
Xiaojun Zhang,Si Chen,Hanxiang Xu,Shuai Zhang,Zhongyong Yan,Jian Wang
Semicarbazide (SEM) is a side-chain metabolite of the antibiotic drug nitrofurazone (NFZ) and is employed as a conclusive marker for the use of banned NFZ. Recent studies have shown that SEM in aquatic crustaceans can be derived natively or from other sources. The presence and distribution of endogenous SEM within aquatic crustaceans is examined in this paper, which finds that the SEM content varies amongst the muscle, shell, and viscera of various prawn and crab species within the range of 0.35–26.62 ng g–1. The effects of heating and hypochlorite treatment on SEM levels were examined. The results indicate that thermal processing introduced a more significant impact, resulting in a maximum SEM value of 15.48 ng g–1 in a sample of shell of Portunus trituberculatus crab, while SEM levels in muscle samples were not affected by the duration of heating. Though 6% active chlorine treatment led to SEM production ranging between 39.9 and 196.4 ng g–1 in muscle samples from various crustaceans, SEM is unlikely to originate from hypochlorite or chlorine in practice where there are limits to actual chlorine in sanitation water and facilities. 5-Nitro-2-furaldehyde (NF) was proposed as a selective marker to differentiate between endogenous SEM and NFZ-derived SEM in seafood.
研究领域:
食品科学
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海洋生物学
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药物分析
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2020
·
IF 2.463
Jun Dan An,Tian Tian Wang,Yang Fan Shi,Xiang Xia Wu,Yuan Yuan Liu,Jian Zhong Huo,Bin Ding
In recent years, luminescent metal organic frameworks (MOFs) materials have attracted widespread attention in the field of fluorescence sensing. In this work, a novel two-dimensional (2D) micro-porous water-stable coordination material {[Cd(μ 3 -HL) 2 (H 2 O)]⋅2.3H 2 O} n ( 1 , H 2 L = 4,4′-(1H-1,2,4-triazol-1-yl)methylene-bis (benzonic acid)) has been prepared through the powerful solvo-thermal approaches, which has been characterized by single crystal X-ray diffraction analysis, PXRD , scanning electron microscope (SEM) and Transmission electron microscopy (TEM) characterization. Crystal structural analysis also reveals the partly de-pronated μ 3 -coordinated HL − adopted tri-dentate bridging mode, which link these cadmium (II) centers, eventually forming the two-dimensional (2D) micro-porous coordination framework of 1 . 1D channel with diemsionality of 12.216(3) Å × 8.578(11) Å that can be observed along the crystallographic a axis. It is noted that 1 has good chemical stability and maintains structural integrity not only in various solvents, but also in aqueous solutions with pH values varying from 2 to 12. Additionally, 1 has the unique advantages such as high detection sensitivity for Nitrofurazone (NZF) and Cupric ion (Cu 2+ ) (K sv quenching efficiency for NZF: 5.0 × 10 6 M −1 and Cu 2+ 5.6 × 10 6 M −1 ) and low detection limits (NZF: 1.21 μM and Cu 2+ : 1.07 μM), good selectivity and good real-time performance. The result of photo-luminescent experiment demonstrates that 1 can realize high sensitivity and real-time chemical discrimination of antibiotics and ions, and has good reusability. To the best of our knowledge, 1 is the first example of the ultra-stable 2D cadmium (II) micro-porous coordination material as a renewable chemical sensor for NZF and Cu 2+ .
研究领域:
材料科学
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分析化学
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环境科学
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DOI
高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 4
·709-720
·
IF 2.9
Zhou Binbin,Sheng Xingxin,Xie Hao,Zhou Sisi,Huang Lijun,Zhang Zuokun,Zhu Yongbo,Zhong Ming
Nitrofurazone (NF) is a kind of synthetic antibiotic with teratogenic and carcinogenic properties, which is often residual in animal food, so it is of great significance to detect it with high selectivity and rapid. In this study, a molecularly imprinted electrochemical sensor was constructed by electropolymerization for the high-specificity, high sensitivity,and rapid detection of NF in milk. By sequentially modifying reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) on the glassy carbon electrode, the effective surface area and electron transfer ability of the electrode are improved, the double amplification of the electrical signal is realized, and the sensitivity and stability of the sensor are improved. A molecularly imprinted polymer (MIP) is polymerized on the surface of the modified electrode by using NF as a target molecule and o -phenylenediamine ( o –PD) as a functional monomer to improve the specific detection ability of the sensor for NF. The experimental conditions (the number of turns of depositing RGO, the thickness of the MIP, the elution time, and the adsorption time) were optimized to attain the best detection performance of the sensor. Differential pulse voltammetry (DPV) was used to detect NF by redox probe ([Fe(CN) 6 ] 3−/4− ). The constructed sensor exhibited an admirable linear relationship between 5 and 1000 nmol L −1 ( R 2 = 0.9968) and the detection limit was 0.18 nmol L −1 (S/N = 3). Compared to the non-imprinted sensor, the imprinting factor was found to be 3.09. In addition, the sensor also showed excellent repeatability (RSD = 1.17%) and stability, and also showed a good recovery rate in actual sample detection (99.06–101.46%), which is expected to be applied to the detection of other harmful substances in food.
研究领域:
分析化学
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食品科学
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环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）