JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·H425
·
IF 3.12
Chunxiang Chen,Wen Chen,Jinlong Jiang,Lei Qian,Xue Zhang
In this study, nitrofurazone (NF), a commonly used but banned drug in aquaculture, was used as the research model for nitrofuran antibiotics. A poly-alizarin red modified glassy carbon electrode (PAR/GCE) was prepared by electropolymerization. On this modified electrode, NF can produce a sensitive and stable reduction peak. Other nitrofuran antibiotics, such as nitrofurantoin and furazolidone, showed similar responses. The reduction peak of NF was proportional to the concentration within the range of 3.0–50.0 and 50.0–200.0 μM, and the detection limit was 0.33 μM. The linear ranges of furantoin and furazolidone were 10–40 and 50–140 μM, respectively, with corresponding detection limits of 0.73 and 1.56 μM. The modified electrode was used to detect NF in the cultured water sample, and the recovery rate of the sample was 94.5%–104.8%. Thus, the result was satisfactory.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Deyong Kong,Bin Liang,Hui Yun,Jincai Ma,Zhiling Li,Aijie Wang,Nanqi Ren
Antibiotics existing in wastewaters must be degraded to eliminate its antibacterial activity before discharging into the environment. Electrochemical reduction by continuous electrons supply can degrade various refractory pollutants, however, the information about the feasibility and characterization of the cathodic degradation of nitrofurans antibiotic furazolidone (FZD) is scarce. Here, we investigated the degradation of FZD using a poised cathode electrochemical reactor. The cyclic voltammetry (CV) preliminarily proved the feasibility of FZD degradation on cathode. In contrast to the different buffer solutions concentrations, buffer types, and initial FZD concentrations which only had obvious impact on the FZD degradation efficiency, different cathode potentials had significant effects both on the FZD degradation efficiency and degradation products composition. Catholyte PBS could be replaced by Na 2 CO 3 –NaHCO 3 and NaCl buffer solution for the FZD degradation. The cathodic degradation pathway of FZD was proposed based on intermediate products analysis. When the cathode potential was lower than −0.75 V, both the furan ring and oxazole ring of FZD were destroyed to generate linear chain products after N–N bond disconnection, suggesting that the high toxic biological metabolite of FZD, 3-amino-2-oxazolidinone (AOZ) could be detoxified by cathodic degradation. This study suggests that the electrochemical reduction could serve as a potential strategy for the treatment of FZD and AOZ containing wastewater.
研究领域:
环境科学
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微生物学
·
毒理学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Yan-Qiu Zhang,Xiao-Han Wu,Shun Mao,Wen-Quan Tao,Zhuo Li
Antibiotics are one of the emerging contaminants in water, which have a great impact on ecosystems and human health. It has been challenging to simultaneously realize low-cost, rapid, highly sensitive and selective detection of antibiotics with conventional methods. Here, we report luminescent chemosensors for detecting antibiotics in water, based on metal-organic framework (MOF), i.e., zeolitic imidazolate framework-8 (ZIF-8), loaded with rhodamine B (RhB) and fluorescein disodium salt (FSS) dyes. Compared with ZIF-8, the fluorescence signals of RhB@ZIF-8 and FSS@ZIF-8 were significantly improved and presented ultrahigh sensitivity to nitrofurans (NFAs) and tetracyclines (TCs) with fluorescence quenching and fluorescence enhancement in water, respectively. The unique structures and properties of RhB@ZIF-8 and FSS@ZIF-8 lead to outstanding sensitivities in antibiotic detection. For instance, the RhB@ZIF-8 sensor shows the lower limit of detection (LOD) of 0.26 μM to nitrofurantoin (NFT), 0.47 μM to nitrofurazone (NFZ), 0.11 μM to tetracycline (TC), and 0.14 μM to oxytetracycline (OTC); while the FSS@ZIF-8 sensor shows the LOD of 0.31 μM to NFT, 0.35 μM to NFZ, 0.17 μM to TC, and 0.16 μM to OTC. In addition, NFT and TC were also successfully detected by FSS@ZIF-8 in water from real water environment. The results indicate that dye@MOF-based luminescent composites are favorable for antibiotic detection, presenting great potentials in water quality monitoring.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Hualai Wang,Fubin Pei,Chun Liu,Yue Ni,Mingzhu Xia,Shasha Feng,Qingli Hao,Tinghai Yang,Wu Lei
In the paper, a novel fluorescent probe based on Ag 2 S QDs/g-C 3 N 4 composite was synthesized by loading Ag 2 S quantum dots (Ag 2 S QDs) on the surface of g-C 3 N 4 through in-situ synthesis method and developed to detect Nitrofurazone (NFZ) sensitively. The results showed that the linear detection range of Ag 2 S QDs/g-C 3 N 4 to NFZ was 0–30 μM, with a low detection limit of 0.054 μM. The results of time-fluorescence-resolved spectroscopy and UV–vis absorption spectroscopy exhibited that the possible detection mechanism of Ag 2 S QDs/g-C 3 N 4 to NFZ was proposed to be Internal Filtration Effect (IFE). Moreover, Multiwfn wavefunction analysis was employed to uncover the possible interaction between the Ag 2 S QDs/g-C 3 N 4 and NFZ, thereby further revealing the fluorescence detection mechanism from the scale of atoms. Combining experiments and theoretical calculations, we proposed the sensing mechanism of the formation of non-fluorescent ground state complex linked by hydrogen bonds. This work indicated that the Ag 2 S QDs/g-C 3 N 4 composite processed the ability to detect NFZ efficiently and sensitively.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 22
·5011-5017
·
IF 5.227
Jiaxi Yin,Hairong Cui,Ling Lei,Kangbing Wu
Graphene nanosheets (GS) were prepared by ultrasonic exfoliation of bulk graphite in N-methyl-2-pyrrolidone with the assistance of sodium pyrophosphate. The obtained GS suspension was modified on a glassy carbon electrode (GS/GCE), and then functionalized at different voltages (e.g. 1.0, 1.4 and 1.6 V) for 2 min in pH 7.0 phosphate buffer. The electrochemically functionalized GS/GCE (i.e. EGS/GCE) possesses more oxygen-containing groups and a higher defect level. More importantly, the active response area, electron transfer ability and interface adsorption capacity of the EGS/GCE enhanced remarkably. The possible mechanism of the performance enhancement is discussed, and the sensing application of the EGS/GCE in the detection of nitrofurazone (NFZ) is investigated. Compared with the GS/GCE, the EGS/GCE is much more active for NFZ oxidation and greatly increases the detection sensitivity. As a result, a highly sensitive electrochemical detection method has been developed for NFZ, with a detection limit of 2.1 nM. The practical application of the EGS/GCE was tested in fish meat samples, showing good accuracy and feasibility.
研究领域:
分析化学
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材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Ghulam Mujtaba Mari,Weilin Wu,M. Farooque Hassan,Ambreen Laghari,Hidayatullah Soomro,Aisha Talpur,Zhanhui Wang,Xuezhi Yu
Nitrovin, classified as a nitrofuran drug, has been banned in numerous countries due to its carcinogenic effect on human health. However, existing analytical techniques for its detection are laborious, requiring an overnight derivatization process lasting 16 h. In this study, novel haptens were designed to prepare antibodies against aminogunidine (AGD), the metabolite of nitrovin, and then developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) method to detect AGD from fish samples without derivatization for the first time. Computer-assisted molecular modeling predicted the efficiency of novel haptens by conformational studies and electronic analysis. The results were consistent with the predicted results in producing high-affinity monoclonal antibody (mAb) against AGD. The half-inhibition concentration (IC 50 ) value of icELISA was 0.80 ng mL −1 for AGD, with a limit of detection of 0.27 μg kg −1 and recoveries of 89.8%–97.2%. The results showed that this new hapten design and synthesis provided a great prospect to obtain high-affinity mAb for AGD. This developed icELISA substantially avoided the conventional and lengthy derivatization process and was an effective method for the direct detection of AGD in fish tissue without derivatization.
研究领域:
药理学
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毒理学
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分析化学
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DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
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2019
·
IF 2.29
Yuxin Bao,Ali Farajtabar,Min Zheng,Hongkun Zhao
Solubilities of 5-nitrofurazone (form γ ) in aqueous co-solvents mixtures of isopropanol, N -methyl pyrrolidone (NMP), ethanol and dimethyl sulfoxide (DMSO) were experimentally investigated via the saturation shake-flask technique at temperatures from 278.15 K to 323.15 K under ambient pressure of p = 101.2 kPa. The solubility of 5-nitrofurazone increased to a maximum value with 100% mass fractions of NMP and DMSO in aqueous solutions of NMP and DMSO, while with 80% mass fractions of isopropanol and ethanol in aqueous solutions of isopropanol and ethanol. The solids equilibrated with liquor were characterized by X-ray power diffraction, indicating that no solvate formation or polymorphic transformation occurred. The Jouyban-Acree model was accepted to correlate the obtained solubility. The acquired highest relative average deviation and root-mean-square deviation values were, respectively, 5.62 × 10 −2 and 11.18 × 10 −4 . Quantitative values for local mole fractions of NMP (ethanol, DMSO, DMF or isopropanol) and water nearby 5-nitrofurazone were obtained by the method of Inverse Kirkwood–Buff integrals. The parameters of preferential solvation were positive for isopropanol/ethanol in the isopropanol/ethanol mixtures in intermediate compositions, and for NMP/DMF/DMSO in the NMP/DMF/DMSO mixtures in intermediate and NMP/DMF/DMSO-rich compositions, which indicated that 5-nitrofurazone was preferentially solvated by the co-solvents. Linear solvation energy relationships were used to model the solvent effect through the analysis of correlations between solubility data and solvent polarity quantified by suitable descriptors.
研究领域:
药学
·
化学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2022
·Vol. 87
·期 7
·3318-3328
·
IF 3.693
Shuai Yan,Yongyu Li,Yankun Peng,Shaojin Ma,Donghai Han
Residues of veterinary antibiotics in honey may be damaging to human health. Surface-enhanced Raman scattering spectroscopy (SERS) is an emerging technology widely applied in food safety. SERS has advantages of enabling fingerprint identification and fast detection, as well as does not require complex pretreatment. Considering the overuse of nitrofurans in honeybee breeding, SERS combined with spectral preprocessing was used to detect nitrofurantoin in honey. By using standardized experimental procedures and improved spectral correction methods, the lowest detection limit of nitrofurantoin was 0.1321 mg/kg. A good linear relationship in the partial least squares regression model was found among spiked samples, which allowed prediction of nitrofurantoin content in honey sample ( R C 2 $R_C^2$ = 0.9744; R P 2 $R_P^2$ = 0.976; RMSECV = 1.0353 mg/kg; RMSEP = 0.9987 mg/kg). Collectively, these results reliably demonstrated that quantification is more accurate when spectral preprocessing is better controlled. Therefore, this study indicates that SERS could be further implemented in fast and onsite detection of nitrofurantoin in honey for improved food safety. Practical Application This article presents a novel SERS-based method for the rapid detection of nitrofurantoin residues in honey. The original spectra were corrected by multiple linear regression based on the fitting baseline. This study aims to develop a rapid onsite detection method for toxic hazardous substance residues in food.
研究领域:
食品科学
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分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xu Wen,Xueling Yan,Xiaoling Zheng,Qiming Kou,Lulan Yang,Jiaming Tang,Xingyue Chen,Yong Xie,Tao Le
Nitrofurazone (NFZ) is an antibiotic banned in many countries, as its residue seriously harms the human body. Herein, anti-NFZ aptamers were selected and identified based on the magnetic bead SELEX technique using a ssDNA library with a full length of 90 nucleotides (nt). Five full sequence candidate aptamers (NFZ8, NFZ24, NFZ28, NFZ34, and NFZ70) were obtained by secondary structure analysis. We optimized the entire sequence to obtain a truncated aptamer, a 16 nt sequence (NFZ8-1:5′-GTTCTATTGAAAAAAC-3′) that showed the highest affinity for NFZ (K d = 76.11 nM). The binding site of NFZ and aptamer NFZ8-1 was found to be "GAA" by molecular docking . In addition, utilizing the most special truncated aptamer NFZ8-1 as the identification probe, a graphene oxide fluorescent aptasensor is an innovative for the detection of NFZ residue that showed a wide linear reach from 1.25 to 160 ng/mL and a low limit of detection of 1.13 ng/mL. In the actual water environment sample detection, the recovery rate ranged from 95.21 to 113.66%, and the coefficient of variation ranged from 3.53 to 11.24%. These results demonstrate that the NFZ-truncated aptamer applied to the aptasensor provides a novel methodology for recognizing NFZ residues.
研究领域:
生物化学
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分析化学
·
药物检测
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xiaoyue Yue,Zijun Zhou,Min Li,Mingsha Jie,Baocheng Xu,Yanhong Bai
Developing highly sensitive and visual methods for rapid detection of antibiotics is significant to ensure food quality and safety. To meet the requirement of nitrofuran antibiotics detection, luminescent fusiform Al(III)-containing metal–organic frameworks (Al-MOF) nanosheets were successfully synthesized by one-step hydrothermal method. And then, the nanosheet served as a fluorescent probe to detect nitrofuran via the inner-filter effect mechanism. The developed sensor allowed sensitive and selective detection of nitrofuran with good linear relationships. And, the detection limit (LOD) values were estimated to be 0.53, 0.838 and 0.583 μM for nitrofurazone, nitrofurantoin and furazolidone detection, respectively. The practical application of the proposed system was verified by HPLC in spiked milk samples with satisfying recoveries ranging from 88.14 to 126.21% and low relative standard deviations of 2.85 ~ 8.13%. Moreover, we designed fluorescent test papers for semi-quantitative detection of nitrofuran via naked-eye colorimetric assay. The established method provides an alternative strategy for semiquantitative detection of nitrofuran.
研究领域:
食品安全
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Huiqun Huang,Hongyuan Huang,Jie Yang,Huan Yang,Jing Dai,Zhuo Li,Wen Yao,Xinrong Guo
The misuse of antibiotics has caused serious impacts on food safety and human health, making it crucial to develop rapidly and highly sensitive methods for detecting trace nitrofuran antibiotics (NFs). In this study, phosphorus, nitride-doped carbon nanosheets (PN/CNs) were synthesized using a simple hydrothermal method based on graphitic carbon nitride . This prepared material showed excellent water solubility and stable optical properties . A new fluorescence sensing platform based on PN/CNs was constructed for the highly sensitive detection of four NFs. This sensitivity was mainly attributed to the fluorescence resonance energy transfer (FRET) mechanism. The limits of detection for nitrofurazone , nitrofurantoin , furazolidone and furaltadone were determined to be 13.41, 15.24, 16.37 and 19.94 nM, respectively. The high sensitivity and selectivity of PN/CNs for these four NFs were thoroughly evaluated by the Stern-Volmer equation and FRET quenching efficiency. This proposed method exhibited high sensitivity and can be successfully applied to detect NFs in fish.
研究领域:
食品安全
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Shuyun Bi,Di Shao,Yue Yuan,Rui Zhao,Xu Li
A novel surface-enhanced Raman spectroscopy (SERS) method for the determination of nitrofurazone was developed using AuNPs/ γ -Al 2 O 3 nanoparticles protected by β -cyclodextrin ( β -CD) as substrate prepared in our lab. The optimum experimental conditions were obtained from single factor procedure and response surface modeling. A linear relationship ( I SERS = 508.96 c + 31987.87, c : nmol L −1 , R 2 = 0.996) between SERS intensity and the concentration of nitrofurazone in the range of 3.3 – 667.0 nmol L −1 was established, the limit of detection (LOD) was found at nmol L −1 level (0.37 nmol L −1 by 3 S 0 / S ). The selectivity for the method was studied by the influences of foreign substances on the determination. The recoveries and RSD (n = 5) for the six meat samples were 95.1 % – 104.5% and 2.4 % – 4.8% respectively, which suggesting that the new SERS method was successfully to detecting nitrofurazone.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）