SENSORS
·
2024
·Vol. 25
·期 1
·34
·
IF 3.4
Yalun Dong,Liyue Wang,Wenfang Liang,Jiqin Zhu,Lu Sun,Long Yi
Glutathione (GSH) plays a crucial role in various physiological processes and its imbalances are closely related to various pathological conditions. Probes for detection and imaging of GSH are not only useful for understanding GSH chemical biology but are also important for exploring potential theranostic agents. Herein, we report a fast intramolecular thiol-activated arylselenoamides (FITA)-based fluorescent probe using 2,4-dinitrophenyl alkylthioether as a sulfydryl-selective receptor for the first time. The fluorescence of the probe was low due to the double effects of PET, while the probe exhibits an 86-fold fluorescence enhancement at 460 nm after GSH activation and a detection limit of 0.95 μM. Furthermore, the probe is low-toxic and capable of imaging cellular GSH. This work further expands the design and applicability of theFITA-based platform, offering a new thiol-deprotection strategy for development of fluorescent probes.
研究领域:
生物化学
·
医学诊断
·
化学生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Material Cycles and Waste Management
·
2020
·Vol. 22
·期 5
·1648-1661
·
IF 1.974
Qian Yi,Han Haoyue,Li Long,Qiao Peng,Chang Guozhang
Red mud-based layered double hydroxides (LRM) was synthesized by a co-precipitation route, and then a hybrid with bis(4-nitrophenyl) phosphate (BNPP)-modified LRM material (BRL) was prepared in this paper. Through X-ray diffraction(XRD), Fourier transform infrared spectra (FTIR), nuclear magnetic resonance spectra (NMR), transmission electron microscope (TEM) and energy dispersive spectrometer (EDS) analysis, the results show that BRL is better dispersed than LRM. Then, thermoplastics polyurethanes (TPU) was used as the base material. TPU/LRM and TPU/BRL composites were prepared by a melt-blending method. Cone calorimeter test (CCT) was used to investigate the effect on flame retardancy and smoke suppression. Specifically, the PHRR values of TPU/LRM and TPU/BRL composites were reduced by 41.6% and 52.5% compared with pure TPU sample. The main factor of improved flame retardancy and smoke suppression can be explained by the catalytic carbonization of LRM or BRL in solid phase and the P-radicals (such as PO · and HPO · ) in gas phase which demonstrated by thermo-gravimetric analyzer test (TGA) and char analysis.
研究领域:
材料科学
·
高分子化学
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2014
·Vol. 37
·期 11
·2353-2359
·
IF 1.823
Ganasen Ponnarasy,Khan Maksudur Rahman,Kalam M. Abul,Mahmud Mohd Sabri
This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p -nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K
M and V
max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.
研究领域:
生物化学
·
酶工程
·
光生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Binbin Zhang,Jiao Ma,Kaide Ou,Xin Cong,Hao Zou,Yonggui Liao,Yajiang Yang,Hong Wang
By loading 3-nitrophenylboronic acid (3-NPBA) which can react with saccharide, the fluorescence lanthanide metallogel can emit fluorescence signals at 398, 490 and 546 nm. Thus, a three-dimensional responsive fluorescence sensor array (H 6 L/Tb 3+ /3-NPBA) was prepared to recognize saccharides. Due to the typical pH responsiveness of the fluorescence behavior of the sensor array, it is suitable for H + generation systems, such as the reaction of 3-NPBA with cis -diol of saccharides. The response of fluorescence signal intensity to 15 different saccharides was measured, and the qualitative discrimination of those saccharides was investigated by principal component analysis (PCA). The results showed that PCA elliptic clusters did not overlap, even though the concentration of the saccharides was as low as 3 mmol/L, and the discrimination rate of each saccharide reached 100 %. The “double blind” experiment indicated that the sensor system has good ability to discriminate unknown component samples. Furthermore, the discrimination rate of fructose/glucose mixtures with varied mole ratios also reached 100 %. The method of Euclidean distance confirmed that there was a good linear correlation between PCA elliptic clusters of fructose and its concentration, which could realize the quantitative analysis of fructose. The results of PCA indicated that the elliptic clusters of tea drinks with various sugars and commercially available sugar-containing drinks were loosely distributed, which further confirmed that the sensor system can effectively discriminate the saccharides under the interference of complex components.
研究领域:
化学传感器
·
生物分析化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 25
·6945-6956
·
IF 3.125
Kumar Saroj,Kumar Dileep,Ahirwar Rajesh,Nahar Pradip
Bioconjugation and functionalization of polymer surfaces are two major tasks in materials chemistry which are accomplished using a variety of coupling agents. Immobilization of biomolecules onto polymer surfaces and the construction of bioconjugates are essential requirements of many biochemical assays and chemical syntheses. Different linkers with a variety of functional groups are used for these purposes. Among them, the benzophenones, aryldiazirines, and arylazides represent the most commonly used photolinker to produce the desired chemical linkage upon their photo-irradiation. In this review, we describe the versatile applications of 4-fluoro-3-nitrophenyl azide, one of the oldest photolinkers used for photoaffinity labeling in the late 1960s. Surprisingly, this photolinker, historically known as 1-fluoro-2-nitro-4-azidobenzene (FNAB), has remained unexplored for a long time because of apprehension that FNAB forms ring-expanded dehydroazepine as a major product and hence cannot activate an inert polymer. The first evidence of photochemical activation of an inert surface by FNAB through nitrene insertion reaction was reported in 2001, and the FNAB-activated surface was found to conjugate a biomolecule without any catalyst, reagent, or modification. FNAB has distinct advantages over perfluorophenyl azide derivatives, which are contemporary nitrene-generating photolinkers, because of its simple, single-step preparation and ease of thermochemical and photochemical reactions with versatile polymers and biomolecules. Covering these aspects, the present review highlights the flexible chemistry of FNAB and its applications in the field of surface engineering, immobilization of biomolecules such as antibodies, enzymes, cells, carbohydrates, oligonucleotides, and DNA aptamers, and rapid diagnostics.
An overview of the FNAB-engineered activated polymer surfaces for covalent ligation of versatile biomolecules
研究领域:
生物化学
·
材料化学
·
医学诊断
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DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2017
·Vol. 75
·期 5
·565-573
·
IF 1.322
Zhang Xin-guo,Liu Jin-wen,Tang Peng,Liu Zi-yu,Guo Guang-Jun,Sun Qiao-Yun,Yin Jian-jun
Adenosine deaminase (ADA) is an enzyme widely distributed from bacteria to humans. ADA is known as a potential therapeutic target for the treatment of lymphoproliferative disorders and cancer. Endophytes are endosymbionts, often bacteria or fungi, which live within plant tissues and internal organs or intercellular space. Endophytes have a broad variety of bioactive metabolites that are used for the identification of novel natural compounds. Here, 54 morphologically distinct endophyte strains were isolated from six plants such as Peganum harmala Linn., Rheum officinale Baill., Gentiana macrophylla Pall., Radix stephaniae tetrandrae, Myrrha , and Equisetum hyemale Linn. The isolated strains were used for the search of ADA inhibitors that resulted in the identification of the strain with the highest inhibition activity, Aspergillus niger sp. Four compounds were isolated from this strain using three-step chromatography procedure, and compound 2 was determined as the compound with the highest inhibition activity of ADA. Based on the results of 1 H and 13 C NMR spectroscopies, compound 2 was identified as 3-(4-nitrophenyl)-5-phenyl isoxazole. We showed that compound 2 was a new uncompetitive inhibitor of ADA with high cytotoxic effect on HepG2 and SMCC-7721 cells (the IC 50 values were 0.347 and 0.380 mM, respectively). These results suggest that endophyte strains serve as promising sources for the identification of ADA inhibitors, and compound 2 could be an effective drug in the cancer treatment.
研究领域:
药理学
·
生物化学
·
肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2015
·
IF 3.615
Ze Feng,Yujia Xie,Fang Hao,Pingle Liu,He'an Luo
A series of manganese metalloporphyrins were prepared and tested in cyclohexane oxidation. It has been found that the manganese 5,10,15,20-tetrakis(4-nitrophonyl) porphyrin (Mn-TNPP) presents better catalytic performance. The Mn-TNPP was then immobilized on different supports, and characterized by UV–vis, FT-IR, XRD, TG/DTG and ICP. Among these supported Mn-TNPP catalysts, Mn-TNPP/ZnO exhibits the best selectivity to KA oil and higher turnover number in cyclohexane oxidation. The influence of the reaction conditions and the recycle of the catalysts are discussed. Mn-TNPP/ZnO catalyst presents the average cyclohexane conversion of 7.17%, KA oil selectivity of 91.18%, and the average catalytic turnover number of 6.53 × 10 4 after six times of recycle under 150 °C and 1.0 MPa.
研究领域:
催化化学
·
有机合成
·
工业化学
·
材料化学
·
绿色化学
✓ 已验证
DOI
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
·
2018
·
IF 1.338
Wenqian Li,Hao Shi,Huaihai Ding,Liangliang Wang,Yu Zhang,Xun Li,Fei Wang
This paper first describes characterization of two thermostable esterases (ThLip1 and ThLip2) from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum DSM 571. The recombinant esterase ThLip1 was active at 80 °C, pH 6.5 and maintained approx. 85% of original activity after 2 h incubation at 75 °C. Kinetic parameters , K m , V max and k cat / K m for 4-Nitrophenyl caprylate ( p NPC) were 3.52 ± 0.47 mM, 191.18 ± 1.82 μmol min −1 mg −1 and 20.80 ± 0.07 mM −1 s −1 , respectively. The purified recombinant esterase ThLip2 was optimally active at pH 6.5 and 75 °C and it was stable against a pH range of 6.0–8.0 possessing 2 h half-life at 80 °C. Kinetic experiments at 75 °C with p NPC as a substrate gave a K m of 3.37 mM, V max of 578.14 μmol min −1 mg −1 and k cat of 231.2 s −1 . The hydrolysis of linalyl acetate were carried out using ThLip1 and ThLip2 as catalyst, affording linalool yields over 140 mg/l in 10 h.
研究领域:
生物化学
·
微生物学
·
酶学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2017
·
IF 0.795
Dan-Dan TANG,Jin-Yi ZHANG,Xian-Deng HOU,Peng WU
The key to maximize the sensitivity of inner filter effect (IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation and/or emission bands of the fluorophore. In this work, Mn-doped ZnS QDs is chosen for IFE-based detection of β -glucuronidase (GUS), since the excitation spectrum of QDs is perfectly overlapped with the absorption spectrum of the substrate of GUS, namely 4-nitrophenyl- β-D- glucuronide (PNPG). Since absorption spectrum of the enzymatic product of GUS-PNP are almost not overlapped with the excitation and emission spectra of Mn-ZnS QDs, the phosphorescent turn-on determination of GUS can be successfully proposed and carried out. In addition, the excellent room temperature phosphorescence property of Mn-ZnS QDs can eliminate the fluorescence background from biological organisms, which can be effectively applied to biological sample analysis with good anti-interference ability. Accordingly, a simple turn-on phosphorescent assay of GUS was developed with a linear range of 10–300 U L −1 and a limit of detection of 7 U L −1 , and the proposed system was also successfully applied for actual detection of Escherichia coli .
研究领域:
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Le Zhong,Zhongjie Wang,Xiaohong Ye,Jiandong Cui,Ziyuan Wang,Shiru Jia
The catalytic performance of immobilized lipase is greatly influenced by functional support, which attracts growing interest for designing supports to achieve their promotive catalytic activity. Many lipases bind strongly to hydrophobic surfaces where they undergo interfacial activation. Herein, the behavioral differences of lipases with distinct lid structures on interfaces of varying hydrophobicity levels were firstly investigated by molecular simulations. It was found that a reasonable hydrophilic/hydrophobic surface could facilitate the lipase to undergo interfacial activation. Building on these findings, a novel “nest”-like superhydrophobic ZIFs (ZIFN) composed of hydrophobic ligands was prepared for the first time and used to immobilize lipase from Aspergillus oryzae (AOL@ZIFN). The AOL@ZIFN exhibited 2.0-folds higher activity than free lipase in the hydrolysis of p-Nitrophenyl palmitate (p-NPP). Especially, the modification of superhydrophobic ZIFN with an appropriate amount of hydrophilic tannic acid can significantly improve the activity of the immobilized lipase (AOL@ZIFN-TA). The AOL@ZIFN-TA exhibited 30-folds higher activity than free lipase, and still maintained 82% of its initial activity after 5 consecutive cycles, indicating good reusability. These results demonstrated that nanomaterials with rational arrangement of the hydrophilic/hydrophobic surface could facilitate the lipase to undergo interfacial activation and improve its activity, displaying the potential of the extensive application.
研究领域:
生物化学
·
催化
·
材料科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
BMC BIOTECHNOLOGY
·
2018
·Vol. 18
·期 1
·1-11
·
IF 2.605
Li Qi,Wu Tao,Qi Zhipeng,Zhao Linguo,Pei Jianjun,Tang Feng
Background β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides to xylose and to biotransform some saponins by cleaving outer β-xylose. β-D-xylosidase is widely used as one of the xylanolytic enzymes in a diverse range of applications, such as fuel, food and the pharmaceutical industry; therefore, more and more studies have focused on the thermostable and xylose-tolerant β-D-xylosidases.Results A thermostable β-xylosidase gene (xln-DT) of 1509 bp was cloned from Dictyoglomus thermophilum and expressed in E.coli BL21. According to the amino acid and phylogeny analyses, the β-xylosidase Xln-DT is a novel β-xylosidase of the GH family 39. The recombinant β-xylosidase was purified, showing unique bands on SDS-PAGE, and had a protein molecular weight of 58.7 kDa. The β-xylosidase Xln-DT showed an optimal activity at pH 6.0 and 75 °C, with p-nitrophenyl-β-D-xylopyranoside (pNPX) as a substrate. Xln-DT displayed stability over a pH range of 4.0-7.5 for 24 h and displayed thermotolerance below 85 °C. The values of the kinetic parameters K
m
and V
max
for pNPX were 1.66 mM and 78.46 U/mg, respectively. In particular, Xln-DT displayed high tolerance to xylose, with 60% activity in the presence of 3 M xylose. Xln-DT showed significant effects on the hydrolyzation of xylobiose. After 3 h, all the xylobiose tested was degraded into xylose. Moreover, β-xylosidase Xln-DT had a high selectivity for cleaving the outer xylose moieties of natural saponins, such as notoginsenoside R1 and astragaloside IV, which produced the ginsenoside Rg1 with stronger anti-fatigue activity and produced cycloastragenol with stronger anti-aging activity, respectively.Conclusion This study provides a novel GH 39 β-xylosidase displaying extraordinary properties of highly catalytic activity at temperatures above 75 °C, remarkable hydrolyzing activity of xylooligosaccharides and rare saponins producing ability in the pharmaceutical and commercial industries.
研究领域:
生物化学
·
酶学
·
生物技术
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DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2017
·Vol. 33
·期 2
·1-9
·
IF 1.658
Zhang Jian-Fen,Chen Wei-Qing,Chen Hong
In this study, we report the cloning and expression of a functional glucoside 3-dehydrogenase (G3DH) gene from Sphingobacterium faecium ZJF-D6. This gene is 1686 bp in length and encodes a peptide of 562 amino acids. The G3DH gene was successfully expressed in E. coli , and the recombinant enzyme could oxidize glucosides, galactosides and analogues at C-3 position. The sequence and multiple alignment analysis showed that the enzyme has highest identity with G3DHs from Paraglaciecola polaris LMG 21857, Aliiglaciecola lipolytica E3 and Halomonas sp. alpha-15. The recombinant G3DH was purified on Ni–NTA column and exhibited the highest activity at pH 7.6 and 30 °C. It was sensitive to acid and alkali, and showed well thermostability. The SfG3DH could oxidize a wild range of sugars. When recombinant E. coli BL21 cells were used as catalyst, a high rate of conversion to N- p -nitrophenyl-3-ketovalidamine was achieved, and no p -nitroaniline was detected. This process offers a promising approach to fulfill substrate of 3-ketovalidoxylamine A C–N lyase production.
研究领域:
微生物学
·
生物化学
·
酶学
·
生物催化
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DOI
PubMed
高影响因子
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