SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiaoqun Dong,Yingying Su,Ting Lu,Lichun Zhang,Liqian Wu,Yi Lv
In the present work, porous Co 3 O 4 was easily prepared via thermal decomposition of metal-organic frameworks (MOFs). TG, FT-IR, XRD, SEM, TEM and N 2 adsorption-desorption were employed for characterizing the synthesized products. Based on cataluminescence (CTL) transduction principle, three MOFs including dodecahedra ZIF-67, nanosheets packed Co-MOF-71, nanoplate Co-BTC, and their corresponding derivatives Co 3 O 4 were used as sensing materials for H 2 S detection, respectively. The dodecahedra ZIF-67-derived dodecahedra Co 3 O 4 with high specific surface and exposed {110} lattice planes presented superior strong CTL property, and had a good linear relation in the concentration range of 3.3–27.5 μg L −1 with the detection limit of 1 μg L −1 (S/N = 3), while the original MOFs displayed no CTL signal. Eighteen common volatile organic compounds (VOCs) were investigated as possible interference, and the results indicated that MOFs-derived porous dodecahedra Co 3 O 4 would be one of excellent sensing material candidates for monitoring H 2 S with a promising application in routine analysis.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Sheng Chen,Jiayi Fan,Mengya Lv,Chenfeng Hua,Gaolin Liang,Shusheng Zhang
Hydrogen sulfide (H2S), as the third endogenous gasotransmitter, is closely associated with various physiological and pathological processes, whereas many aspects of its functions remain unclear. Effective tools for the accurate detection of H2S in living organisms are urgently needed. We herein reported an internal standard assisted surface-enhanced Raman scattering (SERS) nanoprobe for ratiometric detection of H2S in vitro and in living cells based on the reduction of nitros with H2S. This nanoprobe consists of an internal standard (4-mercaptobenzonitrile, MPBN) embedded core-molecule-shell Au nanoflower (Au@MPBN@Au) as the high plasmonic active SERS substrate and the 4-nitrothiophenol (4-NTP) molecule immobilized on the surface as the H2S recognition unit. With the addition of H2S, the nitros peak (1329 cm–1) decreased. Meanwhile, three obvious new peaks appeared at 1139, 1387, and 1433 cm–1, which were related to the vibration of the dimerized product 4,4′-dimercaptoazobisbenzene (DMAB) of 4-aminothiophenol (4-ATP). However, the peak intensity at 2223 cm–1 derived from MPBN was not influenced by the outer environment. Thus, the H2S level was able to be determined based on the ratio of two peak intensities (I1139/I2223) with a detection limit as low as 0.24 μM. Notably, we have proved that SERS nanoprobe Au@MPBN@Au@4-NTP could ratiometrically image both the endogenous and exogenous H2S in living cells. We anticipate that Au@MPBN@Au@4-NTP could be applied for the study of H2S-related physiological function in the future.
研究领域:
分析化学
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生物医学工程
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分子生物学
·
生物成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Shuhui Zhou,Liping Zheng,Tingxuan Zheng,Haiyan Zhan,Qiuyuan Lin,Jiaoao Wei,Yong Huang
Background: Nephrotic syndrome has a significant impact on global health, often leading to cardiovascular disease and high mortality due to limited effective treatments. This study investigates the efficacy of Shensu IV in a puromycin aminonucleoside (PAN)-induced rat model of nephropathy.Methods: Rat models and in vitro podocyte PAN nephropathy models were established with PAN and treated with Shensu IV. Renal function was evaluated by measuring urine output and protein content, while hydrogen sulfide (H2S) and oxidative stress markers were quantified in serum and podocyte lysates. We conducted histological examination on kidney tissues and analyzed molecular markers (CD2AP, nephrin, and PI3K/AKT pathway) using RT-qPCR and Western blot.Results: Shensu IV significantly improved urine output and proteinuria, and attenuated glomerular damage, fibrosis, and mitochondrial swelling in PAN-treated rats. Mechanistically, Shensu IV enhanced endogenous H2S production, reducing oxidative stress and activating the PI3K/AKT pathway in vivo and in vitro. This facilitated the upregulation of the target genes CD2AP and nephrin, which are critical for maintaining glomerular integrity and improving renal function in PAN nephropathy models.Conclusion: Shensu IV and NaHS confer renal protection primarily by modulating oxidative stress and restoring the integrity of the glomerular filtration barrier through mechanisms involving the enhancement of the PI3K/AKT pathway and modulation of H2S levels. These findings suggest a promising therapeutic potential for these metabolites in the treatment of nephrotic syndrome.
研究领域:
药理学
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肾病学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jingjiao Xie,Huiyuan Gong,Zhihan Du,Chen Hong,Wen Luo
Hydrogen sulfide (H 2 S) plays an important role in various pathophysiological processes, and its concentration is associated with various diseases. As such, the development of new H 2 S probes for the early detection of these diseases is of great significance. To date, a variety of probes have been developed for H 2 S detection, however, most probes exhibit response to other sulfur-containing compounds, resulting in a lack of specificity and weaker anti-interference performance. In this paper, a new flavone-based near-infrared (NIR) fluorophore was synthesized by introducing a 5-[4-(dimethylamino) phenyl] thiophene group at the 2-position of the chromone structure. Moreover, using nitrobenzoate as recognition group, two novel probes (TF-3NO 2 and TF-4NO 2 ) were synthesized to detection of H 2 S in biological systems. Among them, TF-3NO 2 exhibited a more specific fluorescent response to H 2 S compared to TF-4NO 2 . Upon treatment with H 2 S, the fluorescence intensity showed a significant increase, showcasing the high specificity, strong anti-interference capability, near-infrared emission (690 nm), and large Stokes shift (∆λ = 223 nm) of the probe. Moreover, the probe was successfully applied for imaging both exogenous and endogenous H 2 S in cells and mice, and enabled to distinguish changes in the H 2 S concentration in the brains of APP/PS1 transgenic and wild-type mice. The above results indicate that TF-3NO 2 can penetrate the mouse blood brain barrier, and is expected to be a powerful molecular tool for detecting H 2 S in living systems within basic and biomedical research fields.
研究领域:
生物化学
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分子生物学
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生物医学工程
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神经科学
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药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Liyang Gong,Le Chang,Siyu Chen,Xuan Wei,Huiping Du,Jiamin Cheng,Xiaoxuan Chen,Zhang Yuan,Pan Zhao,Meijuan Geng,Hui Yang,Kaiyong Cai,Liangliang Dai
Hydrogen sulfide (H 2 S) gas therapy is beneficial for accelerating wound healing and alleviating the inflammatory process, but is seriously hindered by insufficient delivery and unsustainable release in vivo . This study presents a multifunctional injectable hydrogel, OC@ε-PL-SATO, composed of oxidized hyaluronic acid and N-acetylcysteine (NAC) as an initiator, carboxymethyl chitosan and S-aroylthiooxime modified ε-Poly-( l -lysine) (ε-PL-SATO). ε-PL-SATO is a NAC-responsive H 2 S donor. OC@ε-PL-SATO hydrogel is designed for the desired wound healing process, with rapid gelation (<30 s) and a sustained H 2 S release. After mixing and gelling, H 2 S could be long-term released from the hydrogel and effectively drives macrophages toward M2 polarization, thereby ameliorating the inflammatory response. Revealed by transcriptome analysis, the underlying mechanism is that OC@ε-PL-SATO hydrogel releasing H 2 S inhibits LPS-mediated inflammatory responses in RAW264.7 cells by interfering with phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling and NF-κB activation. Furthermore, the OC@ε-PL-SATO hydrogel effectively eliminates the bacterial burden and alleviates the accompanying inflammation in a rat model of cutaneous wound infection. Importantly, the sustained generation of H 2 S gas significantly promotes angiogenesis and collagen deposition, ultimately accelerating the wound repair. In conclusion, this study provides a multifunctional injectable hydrogel with rapid gelatinization and continuous H 2 S release for accelerating the infected wound healing.
研究领域:
生物材料
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药剂学
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伤口愈合
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免疫学
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炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 5
·期 1
·388-399
·
IF 12.958
Zhao Cheng,Guo Jiahui,Zhang Han,Nie Min,Yang Caihong,Zhu Yujuan,Zhao Yuanjin,Zhong Liping
Diabetic wounds have become a major clinical problem that cannot be ignored. Gases, such as hydrogen sulphide (H 2 S), have demonstrated value in inducing angiogenesis and accelerating wound healing, while their effective delivery is still challenging. Here, inspired by the continuous-independent hollow structure of bamboo, we propose novel gasotransmitter microfibres with septal H 2 S bubbles using microfluidic spinning for diabetic wound healing. Benefitting from the exact control of microfluidics, gasotransmitter microfibres with different bubble sizes and morphologies could be generated successfully and continuously. Under the dual effects of drugs in the shell and gas in the core, the wound healing process could be accelerated. Furthermore, the controllable release of drugs could be achieved by adding responsive materials into the microfiber shell, which would promote continuous effects of contents on demand. Based on in vitro and in vivo studies, we have proven that these gasotransmitter microfibres have a positive impact on inducing angiogenesis and promoting cell proliferation during wound healing. Thus, it is believed that the bamboo-inspired gasotransmitter microfibres will have important value in gasotransmitter research and clinical applications. Graphical Abstract The bamboo-inspired microfibres are presented through microfluidics with features of independent chambers for storing and controlled release of hydrogen sulphide (H 2 S) to the diabetic wound. Even if it is partially damaged, it will not affect the overall gas storage and utilization. Thus, it contributes to improvements in basic research and the transformation of gasotransmitters.
研究领域:
生物医学工程
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材料科学
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药学
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组织工程
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Xuetao Zhang,Yunxue Sun,Xinru Zhao,Yimei Bai,Hui Wang,Jing Zhang,Xiaoping Jiang,Meng Gao
As the significant role of hydrogen sulfide (H 2 S) in the fields of health, safety and environmental protection, the exploitation of portable sensing platforms for real-time H 2 S monitoring with versatile antibacterial function is highly anticipated. Herein, a multifunctional fluorescence film sensor (Zr-MOF&RB-P@F) with self-antibacterial performance was constructed by electrospinning method for convenient and accurate detection of H 2 S. Ratiometric sensing process with color changes could be achieved in this system by utilizing the Zr centered metal-organic framework (Zr-MOF) as fluorescent responder and rose bengal modifed polymer (RB-P) as reference unit. As anticipated, the flexible film exhibited low detection limit of 22.4 μM, superior selectivity, visual recognition ability and improved photostability, demonstrating the availability of the design strategy. In addition, excellent antibacterial ability of the film was endowed by the photosensitive RB-P, realizing an antibacterial efficiency of 87.5%. Moreover, the obtained flexible sensing film was able to evaluated the food freshness in real-time and simultaneously decrease their bacterial deterioration, which revealed great potential as intelligent packaging in food safety field. This research provided a valuable reference for the design of flexible multifunctional sensing material, and gave a promising platform for the comprehensive security protection applications.
研究领域:
化学传感
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材料科学
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环境监测
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食品安全
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抗菌技术
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荧光材料
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Fan Yang,Rui Shu,Bin Li,Wenyu Dai,Wanxi Chen,Jiamin Sun,Ding Bai,Weizhong Yang,Yi Deng
Persistent activation of NLRP3 inflammasomes in infectious diabetic wounds lead to uncontrolled inflammation. Hydrogen sulfide (H 2 S) gas therapy shows promise in inhibiting inflammasomes. However, on-demand delivery of H 2 S is challenging, as its biological characteristics are Janus-faced. Herein, we present the anti-inflammasome bio-heterojunction (AI-bioHJ), comprising FeS, Cu 2 O, and glucose oxidase (GOx), capable of H 2 S gas evolution and in situ self-transformation to accelerate diabetic wound healing. In this system, AI-bioHJ release H 2 S to inhibit NLRP3 inflammasomes and recycle excess H 2 S through a reaction between Cu 2 O and H 2 S, maintaining a low H 2 S concentration while upgrading type I FeS/Cu 2 O to Z-scheme FeS/CuS bioHJ, and markedly enhancing phototherapy. Additionally, GOx depletes local glucose to generate H 2 O 2 , promoting Fenton-like reactions and rapid pathogenic elimination. In vivo and in vitro assessments confirm AI-bioHJ effectively eliminate pathogen, inhibit inflammasomes and facilitate diabetic wound healing. Transcriptome analysis indicates that the FoxO–autophagy axis may mediate the anti-inflammasome effects. Altogether, our work provides enlightenments into programmed H 2 S-releasing bioHJ for remedying diabetic wounds.
研究领域:
生物材料
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伤口愈合
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炎症
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气体疗法
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xinyue Zhao,Lulu Ning,Xiaoman Zhou,Zhihui Song,Jianjian Zhang,Feng Guan,Xiao-Feng Yang
Hydrogen sulfide (H2S) is a vital endogenous signal molecule that exerts critical physiological functions such as biological regulation and cytoprotection. Despite significant progress in developing H2S donors, site-specific delivery and controllable release of H2S in biological systems remain a key challenge. Herein, we develop new Cys-triggered fluorescent H2S donor Pro-S that is composed of a dicyanoisophorone-based near-infrared (NIR) fluorescent dye and a thiocarbamate moiety. The H2S donor releases H2S under the attack of Cys, accompanied by the release of a fluorescent reporter, which enables the real-time capturing of H2S by fluorescence spectroscopy or microscopy. Pro-S exhibits strong NIR fluorescence enhancement (70-fold), excellent controllable H2S release (30 min), high H2S release efficiency (62%), and well live-cell compatibility, allowing for visualization of H2S release in cells and zebrafish. Moreover, Pro-S presents a good effect of anti-inflammation in RAW 264.7 cells. This work provides a new idea for the design of H2S donors, which may be beneficial to the comprehension of the potential mechanism of inflammation and optimization of treatment strategies.
研究领域:
生物化学
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药理学
·
分子生物学
·
细胞生物学
·
成像科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Rongrong Wu,Chunhui Tao,Xuegang Chen,Ying Ye,Xihe Yue,Yuqiang Huang,Yifan Zhou,Qiuqin Wang
All-solid-state electrodes are potentially suitable as in situ sensors for deep-sea environments because of their pressure resistance, rapid response, and ease of integration. Nonetheless, few studies report the application of such electrodes in hydrothermal systems. In this study, we fabricated a multi-parameter chemical sensor (MPCS) incorporating self-made reference, pH, redox (Eh), and H2S electrodes. During the Chinese Dayang 34I and 49II cruises at the Southwest Indian Ridge (SWIR), the assembled MPCS, equipped with a deep-sea towed camera system, was continuously monitored in situ at depths exceeding 2,000 m. The potential anomalies of the Eh, H2S, and pH electrodes were 49, 98, and 18 mV, respectively, in the Longqi hydrothermal plume area. In the undetected area, the MPCS showed slight anomalies, indicating the presence of an unknown hydrothermal plume at the site. All potential anomalies of the MPCS were consistent with those of commercial sensors (methane, turbidity, and temperature sensors). The test results suggested that the MPCS can sensitively detect changes in chemical quantities caused by hydrothermal fluid, and it provides an effective technique for exploring hydrothermal vents.
研究领域:
海洋科学
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环境化学
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传感技术
·
海洋学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-11
·
IF 5.3
Wang Sailan,Huang Ling,Ma Guangran,Xu Fugang
A hybrid material GO@Ag composed of graphene oxide (GO) decorated with silver nanoparticles (AgNPs) was prepared for sensitive detection of hydrogen sulfide (H 2 S), a food spoilage indicator and a toxic gas. The GO@Ag nanocomposite demonstrated excellent oxidase-like activity and Raman signal enhancement capability, which can effectively catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to produce oxidized TMB (ox-TMB) with blue color and strong surface-enhanced Raman scattering (SERS) signals. The presence of H 2 S reacts with AgNPs on the GO surface to form Ag 2 S, which inhibits the nanozyme activity and SERS enhancement activity of the GO@Ag, resulting in decreased absorbance value and SERS signal, thereby achieving colorimetric and SERS dual-mode detection of H 2 S. The method exhibits a wide linear range of 10 nM ~ 50 μM (colorimetry) and 5 nM ~ 50 μM (SERS) and low detection limits of 9 nM (colorimetry) and 0.9 nM (SERS) with simple operation, good selectivity, and high reproducibility. Furthermore, the composite material has been successfully applied to the detection of H 2 S in actual fish samples, providing a new technical means for gas sensing in food safety monitoring. Graphical
研究领域:
纳米技术
·
分析化学
·
食品安全
·
材料科学
·
传感器
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Guobin Mao,Yuan Zeng,Chunmin Qiu,Guangmiao Ding,Leyao Li,Lixin Ma,Junbiao Dai,Wen Yin,Yingxin Ma
Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H 2 S) is produced during spoilage-related processes, abnormal H 2 S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S 2− ) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S 2− increased, the fluorescence intensity of Si/CdTe NPs at λ em = 488 nm increased, while the fluorescence intensity at λ em = 620 nm was quenched. The fluorescence intensity ratio (F 620 /F 488 ) was negatively linearly correlated to S 2− concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H 2 S concentration using a novel concept to ensure the freshness and safety of high-protein foods.
研究领域:
食品科学
·
分析化学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）