ACS Applied Bio Materials
·
2023
·
IF 4.7
Cheng Zhang,Mengna Liang,Congying Shao,Ziwei Li,Xue Cao,Yongxiang Wang,Yanan Wu,Shun Lu
Visual detection of mercury ions and glutathione is of great significance to public health and environmental issues. Herein, we developed a fluorescent sensor (l-Cys/CuNCs@ESM) based on the eggshell membrane (ESM) and red-emitting copper nanoclusters (CuNCs) by the in situ strategy via l-cysteine (l-Cys) as the reducing and protective agent for mercury ions and glutathione sensing visually. The as-prepared fluorescent product had good stability, portability, large Stokes shift (250 nm), and long fluorescence lifetime (7.3 μs). Notably, the l-Cys/CuNCs@ESM exhibited a specific fluorescence quenching response toward Hg2+. Moreover, the interaction between glutathione (GSH) and Hg2+ could subsequently recover the fluorescence effectively. Inspired by this “on-off-on” switch, the l-Cys/CuNCs@ESM was applied as the dual-sensing system for visual detection of mercury ions and glutathione integrating with the portable smartphone. The limit of detection (LOD) of Hg2+ is 1.1 μM for visualization and 0.52 μM for the fluorescence spectrometer. The corresponding LODs of GSH are 2.8 and 0.59 μM, respectively. This platform presents significant sensitivity, specificity, and stability, offering a promising potential for real-time/on-site sensing.
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高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiupei Yang,Jing Yang,Maoxue Zhang,Ya Wang,Baowen Zhang,Xiaolin Mei
Tiopronin (TPN) protected gold-silver bimetallic nanoclusters (TPN-AuAg NCs) were synthesized in an aqueous medium by a one-pot method. The synthesized TPN-AuAg NCs are spherical with an average diameter of 1.75 ± 0.5 nm and can emit bright fluorescence in the red spectral range ( E m = 670 nm). Based on the “on-off-on” process of the nanoclusters' fluorescence, a fluorescent probe for sequential detection of Fe 3+ and ascorbic acid (AA) was proposed. After adding Fe 3+ ions into the TPN-AuAg NCs solution, a composite Fe 3+ @TPN-AuAg NCs could be formed. Due to the internal filtering effect (IFE) between Fe 3+ and TPN-AuAg NCs, the fluorescence of the nanoclusters was quenched. Subsequently, when AA was continuously added to the fluorescence quenching system, based on the reduction effect of AA on Fe 3+ in the complex, the fluorescence of Fe 3+ @TPN-AuAg NCs could be sensitively turned on. The detection limits of the fluorescence titration measurements calculated using the equation 3σ/k, were 150 nM (Fe 3+ ) and 60 nM (AA), respectively. The proposed method has been successfully applied to detect Fe 3+ and AA in human serum samples.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Xinli Li,Mengru Chen,Yanbao Zhao,Lei Sun
In this work, S S bond-doped porous silica nanoparticles with CuS nanocores (CuS/OS) were prepared as drug carriers for chemical-photothermal synergistic therapy and redox/pH/light triple-response drug release. The synthesized CuS/OS carriers have dendritic pore structure with particle size of about 100 nm. Tiopronin (MPG) was used as model drug to evaluate the release performance of the carrier. Chitosan (CS) and green fluorescent curcumin (Cur) were used as plugs to seal the loaded drug, which endows the CuS/OS carrier with pH-responsive release and monitoring properties. The obtained MPG-loaded CuS/OS-CS-Cur has pH/GSH/NIR triple responsive release and redox-responsive degradation. The introduction of CuS nanocores gives the CuS/OS system a good photothermal therapy function. The CuS/OS-CS-Cur and its degradation products have good biocompatibility.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 5
·1017-1029
·
IF 2.366
Tian Ruochen,Suo Hao,Zhang Shuting,Sun Baoshan
A series of thiol-nucleophiles, captopril, l -cysteine, and tiopronin, were introduced to the degradation reaction of grape seed extracts and the eight new bioactive flavan-3-ol derivatives were observed. Then, these derivatives were successfully separated and prepared in large quantity by high-speed counter-current chromatography (HSCCC) and semi-preparative HPLC under the optimized separation conditions. The appropriate two solvent systems consisted of n- hexane – ethyl acetate – methanol – water (0.05:1.5:0.5:1.2, v/v/v/v) and n- hexane – ethyl acetate – water (1:10:10, v/v/v) with head – tail mode were used for HSCCC two-step separation of captopril degradation products. N- butanol – ethyl acetate – methanol – water (2:4:0.5:6, v/v/v/v) with a combination of tail – head and head – tail elution modes in HSCCC run of l- cysteine degradation products, and n- hexane – ethyl acetate – methanol – water (0.15:1.6:0.4:1, v/v/v/v) was applied with head – tail and tail – head elution modes in HSCCC separation of tiopronin degradation products. The structures of these derivatives were confirmed by MS and 1 H NMR analysis. Moreover, a systematic comparison of antioxidant activity of all the eleven degradation products was first implemented and found that most of the derivatives had stronger antioxidant activity than free catechins.
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Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 16
·3415-3421
·
IF 5.066
Haiqin Wu,Lan Jin
In this study, new facile, economical and fluorescent ultrathin films (UTFs) for detection of tiopronin are developed. The UTFs have been fabricated by combining the alizarin red S–boric acid adduct (ARS–H3BO3) with MgAl layered double hydroxide (LDH) nanoparticles through the layer-by-layer (LBL) assembly technique. UV-vis, fluorescence spectroscopy, XRD, SEM and AFM have been adopted to monitor the assembly process. The UTFs display a uniform morphology and a periodic layered structure. Using ARS as a probe, which does not emit fluorescence by itself but displays fluorescence when complexed with boron, the assembled ARS–H3BO3/LDH UTFs display a high luminescence response to tiopronin. In the presence of Cu2+, the fluorescence of UTFs was quenched, which is attributed to the complexation between Cu2+ and ARS. Upon adding tiopronin to the UTF–Cu system, tiopronin would form a complex with Cu2+ preferentially, leading to the increased fluorescence of UTFs. Based on the above mechanism, a turn-on fluorescent ensemble for tiopronin is developed. A linear response was obtained in the range of 0–80 ng mL−1, with a detection limit of 0.47 ng mL−1. Compared with ARS and other analytes, the tighter binding of tiopronin to Cu2+ led to an assay with high specificity. Therefore, this work provides a facile LBL strategy for the fabrication of a solid state sensor based on ARS dye for detection of tiopronin.
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INORGANICA CHIMICA ACTA
·
2017
·
IF 2.002
Xiujuan Jiang,Hailong Wang,Yonggang Feng,Erxing Gu,Xiaoming Liu
Three diiron carbonyl complexes with different carbonyl ligands have been synthesized. Pentacarbonyl and tetracarbonyl complexes 2 and 3 were prepared by the substitution of the bound CO of complex 1 by PPh 3 . The photo-induced CO-releasing behaviours of these complexes correlate linearly to their reduction potentials and further to the energy level of their LUMOs. The more negative the reduction potentials, the more reactive the complexes. The photo-induced CO-release can be further promoted when a nucleophile is presented such as thiopronin.
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Science Advances
·
2025
·Vol. 11
·期 43
·
IF 12.5
Hao Tang,Xiaomeng Zhao,Mengsi Sun,Jie Qi,Zhihao Huang,Yihang Zhu,Yingmin Zeng,Yong Xia,Zhi Luo,Xingyu Jiang
No clinically available antibiotic adjuvants can overcome metallo-β-lactamase (MBL) resistance in carbapenem-resistant Gram-negative ESKAPE pathogens. Divalent cations in MBL and quasicrystalline lipopolysaccharides (LPS) are key determinants of acquired and intrinsic resistance in these bacteria. Chemically depriving these cations would block multiple resistance pathways simultaneously and restore carbapenem susceptibility. Here, we chemically engineer a class of anionic adjuvants to tailor their interactions with MBL, enhancing the enzymatic inhibition by up to ~5885-fold compared to parent compounds. An orthogonal screen identifies the tiopronin-engineered anionic adjuvant (TINA), which achieves complete rescue of all tested carbapenems against diverse New Delhi metallo-β-lactamase-1–producing Gram-negative ESKAPE bacteria. TINA targets both MBL and LPS, depriving divalent cations to inactivate MBL and damage membrane collaboratively while also preventing resistance evolution. Moreover, TINA’s anionic nature minimizes serum adsorption, enabling safe and effective in vivo treatment of bacterial pneumonia. This work provides an innovative chemical biology strategy leveraging anionic materials to combat superbugs globally.
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高影响因子
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