JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Wen Li,Jin-Xuan Fan,Ji-Yan Qiao,Qi-Wen Chen,Yun-Xia Sun,Xian-Zheng Zhang
The endogenous H 2 S plays an important role in the occurrence and development of colon cancer , and is related to the abnormal blood vessels. Here, we reported on a sulfhydryl hyaluronid-based hydrogel (HA-SH) synthesized by amide reaction and further obtained a bacterial hydrogel by loading Thiobacillus denitrificans to the hydrogel for targeting adhesion to the colon. It was found that the loaded bacteria in HA-SH hydrogel can scavenge excess H 2 S in colon cancer, then promote tumor vascular normalization and improve the delivery of chemotherapy drug CPT to inhibit tumor progression. Both in vivo and in vitro experiments show that the self-crosslinked bacterial hydrogel has satisfactory effects in inhibiting tumor progression and promoting tumor vascular normalization in colon cancer. This study presents an efficient method to target the colon and consume overexpressed H 2 S in colon cancer to inhabit tumor progression, providing a new way for oral drug treatment of colon cancer.
研究领域:
肿瘤学
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药学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Su Yan,Guanyu Zheng,Xiaoqing Meng,Lixiang Zhou
Schwertmannite and jarosite, two secondary Fe(III)-hydroxysulfate minerals abundant in acid-mine drainage, can be biosynthesized by an Fe-oxidizing bacterium Acidithiobacillus ferrooxidans , but the differences of their catalytic activities in heterogeneous Fenton-like reactions are still unclear to date. In the present study, the catalytic activities of biosynthesized schwertmannite and jarosite were compared through investigating the heterogeneous Fenton-like degradation of a target organic pollutant phenol. Results showed that these two catalysts efficiently catalyzed heterogeneous Fenton-like degradation of phenol, and the degradation in both systems consisted of an induction period (first-stage) and a followed rapid degradation stage (second-stage). Schwertmannite exhibited a much higher catalytic capacity than jarosite, because of its shorter induction period and higher kinetic rate ( k ) of the second-stage, but jarosite catalytically degraded phenol in a much wider pH range (pH 3.0–7.5) over schwertmannite (pH 3.0–4.5). Based on the results of radical scavenger effect, H 2 O 2 decomposition, Fe leaching and X-ray photoelectron spectroscopic (XPS) analysis, similar catalytic mechanisms were proposed in schwertmannite/H 2 O 2 and jarosite/H 2 O 2 systems, in which the induction period can be ascribed to the activation of catalysts by H 2 O 2 and the second-stage was mainly attributed to the homogeneous Fenton reaction in bulk solution. During the catalytic degradation of phenol, catechol was firstly accumulated and was then further decomposed into smaller molecular organic acids. The repeated uses of schwertmannite and jarosite for phenol degradation manifested their stability and reusability as Fenton-like catalysts for the degradation of organic pollutants.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xuqian Wang,Yi Xie,Kun Chen,Likexin Yi,Yabo Wang,Yongkui Zhang
Ferruginous rhodochrosite with high impurity is difficult to be utilized by traditional beneficiation process due to the complex characteristics. Herein, a bioleaching process driven by Acidithiobacillus ferrooxidans was proposed to recover Mn and Fe in ferruginous rhodochrosite with economic and environmental benefits. The biogenic Fe 3+ , H + and extracellular polymeric substances produced in bioleaching process significantly promoted the metal recovery and reduced the H 2 S emission. Optimal metal leaching efficiencies (95.94% of Mn and 97.54% of Fe) were achieved in bioleaching group. Furthermore, the Mn and Fe mixed leaching solutions could be directly adopted to fabricate Mn-Fe oxide based materials (MFO). When applied as catalysts for peroxymonosulfate (PMS) activation, the as-synthesized MFO performed well for sulfathiazole (STZ) degradation with a 98.66% removal efficiency in 40 min. Both radical process and non-radical process occurred in the advanced oxidation process, which induced the generation of abundant active species of ̇OH, SO 4 ̇ − and 1 O 2 . Meanwhile, charge transfer also contributed to STZ degradation. Favorably, MFO-3 exhibited good reusability in recycle study and showed adaptability towards various natural water matrixes. This research not only provided novel insights into the utilization of recalcitrant ferruginous rhodochrosite resources, but also enriched the strategy of PMS activator design for environmental remediation.
研究领域:
环境科学
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微生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Results in Engineering
·
2025
·
IF 7.9
Baojun Yang,Xin Zhang,Yuling Liu,Xin Sun,Yang Liu,Jun Wang,Shitong Liu,Rui Liao,Guanzhou Qiu
Arsenopyrite is an important gold-bearing sulfide mineral, and pretreatment is essential before gold extraction to maximize gold recovery. Bioleaching, with its advantages of simple operation and environmental friendliness, has received widespread attention over the past decade. However, the low efficiency and long cycle of bioleaching hinder its large-scale application. Humic acid (HA) is a potential substance that can enhance the bioleaching of arsenopyrite and has advantages such as ease of acquisition, and environmental friendliness. Currently, there is a lack of research on the promoting effect and mechanism of HA on the bioleaching of arsenopyrite under the action of mesophile microorganism. This study investigates the effects of HA mediated by Acidithiobacillus ferrooxidans on the arsenopyrite leaching behavior, the valence states and forms of arsenic. The results showed HA can promote the bioleaching of arsenopyrite and shorten the leaching cycle. The addition of 20 mg/L HA can significantly reduce the bioleaching cycle of arsenopyrite from 14 days (without HA) to 6 days. HA can promote the growth of microorganisms and enhance the Fe/S oxidation capacity of microorganisms. Meanwhile, HA can adsorb onto the mineral surface, facilitating the oxidation of arsenopyrite by Fe 3+ adsorbed by HA, thereby promoting the bioleaching of arsenopyrite. This study offers new insights into shortening arsenopyrite bioleaching cycle.
研究领域:
生物冶金
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环境生物技术
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微生物学
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地球化学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Binyuan Gao,Zebin Han,Haina Cheng,Hongbo Zhou,Yuguang Wang,Zhu Chen
Acid mine drainage (AMD) induced by pyrite oxidation is a notorious and serious environmental problem, but the management of AMD in an economical and environmentally friendly way remains challenging. Here, lignin, a natural polymer and abundant waste, was employed as both a bactericide and passivator to prevent AMD formation. The addition of lignin to a mimic AMD formation system inoculated with Acidithiobacillus ferrooxidans at a lignin-to-pyrite weight ratio of 2.5: 10 reduced the combined abiotic and biotic oxidation of pyrite by 68.4 % (based on released SO 4 2− ). Morphological characterization of Acidithiobacillus ferrooxidans revealed that lignin could act on the cell surface and impair the cell integrity, disrupting its normal growth and preventing biotic oxidation of pyrite accordingly. Moreover, lignin can be used alone as a passivator to form a coating on the pyrite surface, reducing abiotic oxidation by 71.7 % (based on released SO 4 2− ). Through multiple technique analysis, it was proposed that the functional groups on lignin may coordinate with iron ions on pyrite, promoting its deposition on the surface. In addition, the inherent antioxidant activity of lignin may also be actively involved in the abatement of pyrite oxidation via the reduction of iron. Overall, this study offered a “treating waste with waste” strategy for preventing AMD formation at the source and opened a new avenue for the management of AMD.
研究领域:
环境科学
·
化学工程
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Cong Huang,Qian Liu,Zhi-Ling Li,Xiao-dan Ma,Ya-Nan Hou,Nan-Qi Ren,Ai-Jie Wang
The denitrification desulfurization system can be used to remediate wastewater containing carbon, nitrogen, and sulfur. However, the relationship between autotrophic and heterotrophic bacteria remains poorly understood. To better understand the roles and relations of core bacteria, an expanded granular sludge bed (EGSB) reactor was continuously operated under autotrophic (stage I), heterotrophic (stage II) and mixotrophic (stages III–VII) conditions with a 490-day period. Stage IV represented the excellent S 0 recovery rate (69.5%). The different trophic conditions caused the obvious succession of dominant bacterial genera. Autotrophic environment (stage I) enriched mostly Thiobacillus , and heterotrophic environment (stage II) was dominated with Azoarcus and Pseudomonas. Thauera, Arcobacter and Azoarcus became the predominant genera under mixotrophic conditions (stage III-VII). Strains belonged to these core genera were further isolated, and all seven isolates were confirmed with denitrifying sulfur oxidation capacity. Heterotrophic strain HDD1 (genus of Thauera ) possessed both the highest sulfide degradation and S 0 recovery rates. Expression levels of cbbM and gltA genes were positively related with the autotrophic and heterotrophic conditions, respectively. NirK gene was highly expressed between log 3.7–log 4.3 during the entire run. Expression of both sqr and soxB genes were closely related with sulfur conversion. More than 57.5% of S 0 recovery rate could be obtained as sqr gene expression was greater than log 3.2, and while, sulfate was the primary form as soxB gene expression higher than log 3.9. The correlation between core microbial genera was very low from network, indicating a complex and non-specific mutualistic network between bacterial functional groups under each nutrient condition, and a stable coexistence state was possibly formed through utilizing each the secondary or waste metabolites in the mixotrophic conditions. This relationship was beneficial to the stability of the microbial community structure in the denitrification desulfurization system.
研究领域:
环境微生物学
·
废水处理
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Mingxing Hu,Hanmin Zhang,Yu Tian
An innovative partial nitrification and short-cut sulfur autotrophic denitrification (PN-SSAD, NH 4 + -N → NO 2 − -N → N 2 ) coupled system in a single-stage SBR was proposed to treat low C/N wastewater with low material and energy consumption. Nearly 50 % alkalinity consumption and 40 % sulfate production were reduced in S 0 -SSAD compared with S 0 -SAD, whereas the autotrophic denitrification rate was increased by 65 %. In S 0 -PN-SSAD, the TN removal efficiency reached almost 99 % without additional organic carbon. Furthermore, pyrite (FeS 2 ) rather than S 0 served as the electron donor to optimize the PN-SSAD process. The practical sulfate production in S 0 -PN-SSAD and FeS 2 -PN-SSAD were about 38 % and 52 % lower than complete nitrification and sulfur autotrophic denitrification (CN-SAD), respectively. Thiobacillus was the major autotrophic denitrification bacteria in S 0 -PN-SSAD (34.47 %) and FeS 2 -PN-SSAD (14.88 %). Nitrosomonas and Thiobacillus played a synergistic effect in the coupled system. FeS 2 -PN-SSAD is expected as an alternative technology for nitrification and heterotrophic denitrification (HD) in treating low C/N wastewater.
研究领域:
环境工程
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微生物学
·
水处理技术
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Nianting Xue,Hao Sun,Shihang Lu,Yizhen Yu,Wenyan Ma,Guangzhou Liu,Wenwen Dou
Microbiologically influenced corrosion (MIC) poses a serious threat to the structural integrity of Cu Ni alloys under marine service conditions. This study proposed a novel antibacterial peptide (Tcs) combined with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to inhibit MIC in 90/10 Cu Ni alloy induced by Desulfovibrio ferrophilus IS5. Bacterial counts, environmental parameters analysis, surface morphological analyses, weight loss measurements, and electrochemical tests were conducted, through which the antibacterial and corrosion inhibition effects were demonstrated. The results showed that 180 ppb Peptide Tcs (180 Tcs) alone exerted almost no inhibitory effect on D. ferrophilus IS5, whereas a significant concentration-dependent enhancement was observed when Peptide Tcs was combined with 20 ppm ADBAC (20 ADBAC). Specifically, the 20 ppm ADBAC +180 ppb Peptide Tcs (20 ADBAC-180 Tcs) combination further reduced sessile bacterial counts by one order of magnitude compared to 20 ADBAC alone, while the weight loss rate and maximum pitting depth decreased by approximately 86 % and 61 %, respectively. These findings provide new insights into optimizing traditional biocide systems with small antimicrobial peptides and developing green, efficient strategies for controlling marine MIC.
研究领域:
材料科学
·
微生物学
·
腐蚀工程
·
海洋技术
·
抗菌肽
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Tang Deping,Xi Yanpeng,Song Wentao,Li Mengjiao,Liu Yali,Lin Yanyan,Zhang Ran,Mao Aihong
Introduction: Currently, there are few investigations on the effect of a synthetic exogenous quorum sensing (QS) agonist on the bioleaching rate of Acidithiobacillus thiooxidans (A. thiooxidans).Methods: We created AHL (N-acyl-homoserine lactone) analogues and investigated their effects on A. thiooxidans biofilm formation, adsorption kinetics, bioleaching, and mechanism.Results: The findings revealed that N-(3-thiolactone)- dodecylamine (Y3) significantly increased the biofilm formation of A. thiooxidans in 96-well plates and sulfur sheets. Adsorption tests revealed that Y3 increased the adhesion rate, adsorption constant, and adsorption efficiency. Bioleaching tests indicated that Y3 boosted bioleaching efficiency, with Ni2+ and Cu2+ bioleaching rates increasing by 49.13% and 33.03%, respectively. Transcriptomic analysis revealed that Y3 increased genes associated with QS pathways and biofilm formation, particularly afeI, which was dramatically elevated 42 times.Discussion: The study laid the groundwork for a better understanding of the mechanics of A. thiooxidans biofilm formation, which could help improve the potential application of A. thiooxidans in bioleaching.
研究领域:
微生物学
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生物技术
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环境工程
·
冶金学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Environmental Science and Technology
·
2023
·Vol. 20
·期 12
·13399-13410
·
IF 3.1
Xiong H.,Peng S.,Zhang B.
Nonionic surfactants with ideal surface properties and lower ecotoxicities have been highly attended in metal bioleaching and pollutant bio-treatment in environmental science field. In this study, both Acidithiobacillus ferrooxidans HX3 and Tween 80 (or Triton X-100) presence in cultures, bio-production and development of schwertmannite as a potential metal scavenger were investigated. To explore their correlations, the resulting ferric oxyhydroxides under various surfactant levels (lower/optimal/higher ones for Gp I/II/III treatments) were identified by many characterization methods. Result showed that after 48 h of normally bacterial reproduction, surfactant-rich cultures with Tween 80 more than 50 mg/L (25 mg/L for Triton X-100) could only achieve down 80% of ferrous oxidation percent and 10 7 cells/mL of cell numbers, while those with lower surfactant level were ahead 12 h. Culture pH values decreased to about 2.4 (or 2.0) from initially 2.8. All initial ferric oxyhydroxides were only pincushion-schwertmannite spheres. When cells sequentially reproduced within 36 h–48 h, presence of surfactants (except for Triton X-100 at 75 mg/L) could induce schwertmannite-pincushion assembling on developed spherical particles with a little of diamond-jarosite. And then during bacterial stability state (48 h–72 h), the pincushion assemblages on developed schwertmannite-spheres would disappear, following occurrence of more jarosite amount. Obviously, schwertmannite stability and morphological evolvement were affected by type and level of the added surfactants. These results will give a convincing proof for understanding biomineralization principles of iron minerals in natural environments with nonionic surfactants.
研究领域:
环境科学
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微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Changjin Ou,Sujuan Yuan,Fujii Manabu,Ke Shi,Mohamed Elsamadony,Juntong Zhang,Juan Qin,Jian Shi,Zhipeng Liao
Anaerobic biotechnology for wastewaters treatment can nowadays be considered as state of the art methods. Nonetheless, this technology exhibits certain inherent limitations when employed for industrial wastewater treatment, encompassing elevated substrate consumption, diminished electron transfer efficiency, and compromised system stability. To address the above issues, increasing interest is being given to the potential of using conductive non-biological materials, e,g., iron sulfide (FeS), as a readily accessible electron donor and electron shuttle in the biological decontamination process. In this study, Mackinawite nanoparticles (FeS NPs) were studied for their ability to serve as electron donors for p -chloronitrobenzene ( p -CNB) anaerobic reduction within a coupled system. This coupled system achieved an impressive p -CNB removal efficiency of 78.3 ± 2.9% at a FeS NPs dosage of 1 mg/L, surpassing the efficiencies of 62.1 ± 1.5% of abiotic and 30.6 ± 1.6% of biotic control systems, respectively. Notably, the coupled system exhibited exclusive formation of aniline (AN), indicating the partial dechlorination of p -CNB. The improvements observed in the coupled system were attributed to the increased activity in the electron transport system (ETS), which enhanced the sludge conductivity and nitroaromatic reductases activity. The analysis of equivalent electron donors confirmed that the S 2- ions dominated the anaerobic reduction of p -CNB in the coupled system. However, the anaerobic reduction of p -CNB would be adversely inhibited when the FeS NPs dosage exceeded 5 g/L. In a continuous operation, the p -CNB concentration and HRT were optimized as 125 mg/L and 40 h, respectively, resulting in an outstanding p -CNB removal efficiency exceeding 94.0% after 160 days. During the anaerobic reduction process, as contributed by the predominant bacterium of Thiobacillus with a 6.6% relative abundance, a mass of p -chloroaniline ( p -CAN) and AN were generated. Additionally, Desulfomonile was emerged with abundances ranging from 0.3 to 0.7%, which was also beneficial for the reduction of p -CNB to AN. The long-term stable performance of the coupled system highlighted that anaerobic technology mediated by FeS NPs has a promising potential for the treatment of wastewater containing chlorinated nitroaromatic compounds, especially without the aid of organic co-substrates.
研究领域:
环境工程
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生物技术
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废水处理
·
化学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Jianping Lu,Baogang Zhang,Song Wang,Pan Gao,Shixiang Wang,Shungui Zhou,Fang Huang,Hailiang Dong
Vanadate [V(V)] reduction, a key process of vanadium(V) cycling and detoxification, is of geochemical interest and environmental importance, which can be driven by reducing minerals solely or by mediated by microorganisms. However, the discrepancies between abiotic and biotic processes remain poorly understood. This study demonstrated that widespread pyrite (FeS2) could reduce V(V) both directly and through microbial mediation by Thiobacillus denitrificans (T. denitrificans), with distinct outcomes. The presence of T. denitrificans accelerated V(V) reduction and improved the long-term reactivity of pyrite. XANES analysis showed that abiotic and biotic reduction resulted in an apparent oxidation state of V at 4.82 and 4.41, respectively. Microbial activity further enhanced electron transfer, supported by nicotinamide adenine dinucleotide, riboflavin and the upregulation of cnorB, nosZ, mtoA, and soxB. δ51V isotope fractionations with enrichment factors of −0.53 ± 0.06‰ (abiotic) and −0.89 ± 0.07‰ (biotic) were determined, with significant difference. Larger δ34S isotope fractionation with only δ13C change was observed in the biotic system. Bioaugmentation/biostimulation confirmed the effectiveness of pyrite in V(V) immobilization, while T. denitrificans further enhanced V(V) removal and colonized well in aquifer sediment. This work advances the understanding of couped geochemical cycling of V and pyrite and highlights the vital role of microorganisms in V remediation.
研究领域:
环境地球化学
·
生物地球化学
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矿物学
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微生物生态学
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环境科学
·
电化学
·
同位素地球化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）