AQUATIC TOXICOLOGY
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2024
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IF 4.1
Xuchun Qiu,Zhen Su,Jiarui Gao,Yiming Cui,Kejun Dong,Kun Chen,Ru-Jin Zhao,Songmei Wang,Tao Wu,Yanhong Shi
Thimerosal (THI) is the most widely used form of organic mercury in pharmaceutical and personal care products, and has become a major source of ethylmercury pollution in aquatic ecosystems. However, knowledge about its potential risk to aquatic species is limited. In this study, zebrafish were exposed to THI for 7 days, and variations in their behavioral traits, brain monoaminergic neurotransmitter contents, and related gene expression were investigated. After the 7-day exposure, THI reduced locomotor activity and thigmotaxis in males but not females. Exposure to THI increased the social interaction between females but decreased that between males. The THI exposure also significantly reduced the serotonin (5-HT), 5-hydroxyindoleacetic acid, dopamine (DA), and 3,4-dihydroxyphenylacetic acid contents in the brain of males, but only significantly decreased the DA content in females. Correlation analysis revealed that the neurochemical alterations in the brain of zebrafish play critical roles in the behavioral abnormalities induced by THI exposure. Moreover, THI also significantly altered the expression of some genes associated with the synthesis, metabolism, and receptor binding of 5-HT and DA in the brain of zebrafish. The differences in these gene expressions between female and male zebrafish exposed to THI seem to be an important mechanism underlying their sex-specific responses to this chemical. This is the first report on the sex-specific effects of THI on behaviors and brain monoaminergic neurotransmitter contents in zebrafish, which can further improve our understanding of its toxic effects on teleost.
研究领域:
环境毒理学
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神经生物学
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生态学
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DOI
PubMed
高影响因子
Analytical Methods
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2017
·Vol. 9
·期 11
·1771-1778
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IF 1.9
Xiaoping Wei,Ting Wu,Yali Yuan,Xionghui Ma,Jianping Li
A highly sensitive molecularly imprinted electrochemical sensor constructed by a novel microporous metal–organic-framework for the detection of organometallic compounds was developed. A monolayer of 4-aminothiophenol (4-ATP) was self-assembled on the surface of a gold electrode, followed by electropolymerization of the 4-ATP-functionalized gold nanoparticles (Au NPs) in the presence of template molecules (thimerosal), forming a microporous molecularly imprinted polymeric (MOF-MIP) film. The obtained MOF-MIP film was characterized by cyclic voltammetry (CV), alternating current impedance spectroscopy, and scanning electron microscopy (SEM), exhibiting a higher specific surface area, faster rate of electron conduction, and more accessible imprinted molecular cavities compared with the MIP film prepared without Au NPs. Therefore, the MOF-MIP electrochemical sensor exhibited a significantly enhanced sensitivity. Thimerosal was determined in the range of 0.8–80 pM with the detection limit of 0.035 pM. The sensor was successfully applied to determine the amount of thimerosal in chloramphenicol eye drop samples, giving rise to further applications in the food industry and environmental field.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Xuchun Qiu,Yibing Zhang,Jiarui Gao,Yiming Cui,Kejun Dong,Kun Chen,Yanhong Shi
Thimerosal (THI) has become a significant source of organic mercury pollutants in aquatic ecosystems, but there is limited information regarding its adverse effects on fish. In this study, zebrafish embryos were exposed to THI at 0 (control), 5.0, and 50 ng/L from 0–5 days post fertilization (dpf), and variations in their survival, development, behavior, free amino acid contents, and the biochemical responses involved in monoaminergic systems were examined. Although THI exposure did not significantly affect the survival, heart rate, or hatching time of zebrafish embryos, it substantially increased swimming velocity (136–154 % of the control) and reduced exploratory behavior (141–142 % of the control) in zebrafish larvae at 5 dpf. Exposure also significantly altered the amino acid contents (51–209 % of the control) and monoamine levels (70–154 % of the control) in zebrafish larvae, some of which displayed significant correlations with behavioral traits. THI significantly elevated dopamine receptor gene expression and monoamine oxidase activity in zebrafish larvae. Adding extra phenylalanine or tryptophan to the E3 medium facilitates the recovery of zebrafish larvae from the abnormal behaviors induced by THI. These findings reveal for the first time that THI exposure at the level of ng/L is sufficient to induce neurobehavioral toxic effects in the early life stages of zebrafish, and disrupting amino acid homeostasis is a critical underlying mechanism. This study provides valuable insights into the toxicity of THI to fish and highlights the importance of assessing its potential risks to aquatic ecosystems.
研究领域:
环境毒理学
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水生生物学
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神经科学
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发育生物学
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DOI
PubMed
高影响因子
Metallomics
·
2023
·Vol. 15
·期 4
·
IF 3.4
Ma Junrong,He Man,Chen Beibei,Hu Bin
Thimerosal (THI) is widely used as an antimicrobial preservative, but can hydrolyze to ethylmercury, causing potentially neurotoxicity. In this work, a THP-1 cell line was used to investigate the biological behavior of THI. An on-line droplet microfluidic chip system combined with time-resolved inductively coupled plasma mass spectrometry was used to quantify Hg in single THP-1 cells. The cellular uptake and elimination behaviors of THI were studied, and the toxicity of THI in terms of redox balance was discussed. The results showed that a small number of cells (<5%) exhibited a high uptake content (>200 fg/cell) for THI, and most of the cells (68.8–85.8% for different exposure groups at 25 h) exhibited a relatively low uptake content (<20 fg/cell). After stopping exposure to THI, the cells showed an elimination process for Hg, which was rapid in the first several hours and gradually slowed down. When the elimination time was 25 h, 7.4–26.3% of the cells in different exposure groups still contained a detectable amount of Hg (>2 fg/cell), indicating Hg could not be eliminated completely, which may cause cumulative toxicity to macrophages. Moreover, it was found that exposure to THI even at 50 ng/mL can cause cellular oxidative stress behavior, leading to an increase in reactive oxygen species level and a decrease in glutathione level. This trend would continue for a period of time after stopping THI exposure. With the elimination of Hg, the redox balance of cells showed a tendency to stabilize and restore, but cannot be restored to normal status, indicating a long-term chronic toxicity of THI to THP-1 cells.
研究领域:
分析化学
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细胞生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2020
·Vol. 27
·期 33
·41437-41445
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IF 3.056
Zhu Chao,Wang Huiqin,Ma Hongrui,Yang Yonglin,Li Fan
Humic-like substances are essential components of soluble organic matter in tannery wastewater. However, the tannery process can promote the abiotic humification in wastewater. Therefore, it is of great significance to clarify the pathway and degree of abiotic humification and the properties of the as-derived humic acid-like (HAL) complex polymers in the tannery process in order to control the refractory organic compounds. In the present study, considering the catechol-Maillard system and commercial humic acid (HA) as control, the polyphenol-Maillard humification in the tannery process was simulated under the catalysis of MnO 2 . Moreover, physicochemical and spectroscopic techniques were used to characterize the separated fractions of HAL further. As a result, it was found that the catechol-Maillard system with small molecule organic matter as precursor had higher humification degree. Furthermore, the ultraviolet-visible (UV-Vis), Fourier transform infrared (FTIR), and excitation-emission matrix (EEM) fluorescence spectrum of humic acid-like 0 (HAL0) derived from it was different from those of humic acid-like 1 and 2 (HAL1 and HAL2) of polyphenol-Maillard system, indicating the differences of polymer structure between them. In the polyphenol-Maillard system, tannin was the skeleton of polymerization or polycondensation reaction, and the high content of N and the H/C value of HAL2 indicated that in adding to amino acids, proteins promoted the humification, forming industry-specific HAL polymers with a high degree of aliphatic nature. Therefore, it can be concluded that controlling the raw materials in the tannery process (especially tannins), in order to reduce the occurrence of abiotic humification may be the key to improve the efficiency of wastewater treatment.
研究领域:
环境科学
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化学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2024
·
IF 2.9
Tian Ye,Liu Zhaoying,Sun Mingna,Duan Jinsheng,Verheecke-Vaessen Carol,Du Daolin,Li Ming
To further promote the pretreatment technique, the immunoaffinity column (IAC), immunoaffinity magnetic (IAM) and multi-component immunoaffinity magnetic (multi-IAM) for mycotoxins were developed and evaluated systematically. Anti-mycotoxin-McAbs had immobilized on the CNBr-Sepharose 4B, carboxyl magnetic bead and protein G magnetic bead to prepare the IAC/IAM/multi-IAM, respectively. The capture efficiencies of mycotoxins (100 ng) reached 92.8% for IAC and exceeded 94.0% for IAM/multi-IAM. The multi-IAM showed more beneficial features in multiple targets, pretreatment time and recycling time than IAC/IAM, which could simultaneously pretreat AFB 1 /DON/ZEN in 15 min with three recycling times. The recoveries of IAC/IAM/multi-IAM coupled with ELISA ranged from 85.2 to 105.1%, with RSD between 4.0 and 13.2%. Moreover, the mycotoxin-positive authentic samples were detected from 1.1 to 749.6 ng/g by ELISA, and 1.2 to 762.7 ng/g by LC-MS/MS, with correlated R 2 exceeding 0.9955. The proposed IAC/IAM/multi-IAM owned the desired performance, such as specificity, accuracy, simpleness, efficiency, cost-effectiveness, simultaneity and environmentally friendly. This study can provide the theoretical basis for three immunoaffinity pretreatment techniques systematically, which may guide their application under different requirements and scenarios. Graphical abstract
研究领域:
食品科学
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分析化学
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免疫学
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产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 19
·4348-4355
·
IF 5.227
Beijun Ang,Xinxin Xu,Liguang Xu,Liqiang Liu,Hua Kuang,Chuanlai Xu
Homocysteine (Hcy) is an amino acid found in the human body and has the potential to predict cardiovascular risk where timely detection and treatment are very important. In this study, we established a method to combine enzymatic hydrolysis with a colloidal gold lateral flow immunoassay (LFIA) for the detection of total Hcy (tHcy) based on the monoclonal antibody (mAb) 3B3 against S-adenosyl-L-homocysteine (SAH). The 50% inhibitory concentration (IC50) for this mAb against SAH was 2.94 nM with a linear range of 0.73–11.75 nM. The developed LFIA could detect SAH with a vLOD and cLOD of 10 nM and 2.07 nM, respectively. For tHcy detection in serum samples, the LFIA strip was able to detect tHcy after enzymatic hydrolysis, and the results correlated well with that of an enzymatic cycling test kit.
研究领域:
临床化学
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免疫学
·
心血管疾病
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）