SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jian-Jie Gao,Bo Wang,Zhen-Jun Li,Jing Xu,Xiao-Yan Fu,Hong-Juan Han,Li-Juan Wang,Wen-Hui Zhang,Yong-Dong Deng,Yu Wang,Ze-Hao Gong,Yong-Sheng Tian,Ri-He Peng,Quan-Hong Yao
Industrial thiocyanate (SCN − ) waste streams from gold mining and coal coking have caused serious environmental pollution worldwide. Phytoremediation is an efficient technology in treating hazardous wastes from the environment. However, the phytoremediation efficiency of thiocyanate is very low due to the fact that plants lack thiocyanate degradation enzymes. In this study, the thiocyanate hydrolase module was assembled correctly in rice seedlings and showed thiocyanate hydrolase activity. Rice seedlings engineered to express thiocyanate degrading activity were able to completely remove thiocyanate from coking wastewater. Our findings suggest that transforming the thiocyanate hydrolase module into plants is an efficient strategy for rapid phytoremediation of thiocyanate in the environment. Moreover, the rice seedlings expressing apoplastic or cytoplasmic targeted thiocyanate hydrolase module were constructed to compare the phytoremediation efficiency of secretory/intracellular recombinant thiocyanate hydrolase. The most obvious finding from this study is that the apoplastic expression system is more efficient than the cytoplasm expression system in the phytoremediation of thiocyanate. At last, this research also shows that the secreted thiocyanate hydrolase from engineered rice plants does not influence rhizosphere bacterial community composition.
研究领域:
环境科学
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代谢工程
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植物生物技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Wen Wang,Liqiang Qi,Song Han,Hejin Yuan
A new chitosan-trithiocyanuric composite (CS@TMT) was synthesized by graft copolymerization using chloroacetyl chloride as the cross-linking agent. Using response surface analysis, the optimal conditions for the synthesis of CS@TMT were determined as: chloroacetyl chloride concentration of 2.0 mol/L, trithiocyanuric acid (TMT) addition of 7.0 g, and reaction temperature of 70 °C. The successful preparation of CS@TMT was confirmed FTIR spectrum, 13C NMR spectrum, SEM mapping, EDS, XRD and XPS. At ambient temperature (25 °C, pH=6.0, Catalyst dosage=1.0 g/L), the maximum Cr(VI) and Pb(II) adsorption amount of CS@TMT were 194.76 and 101.39 mg/g, respectively, compared to CS (97.15 and 69.76 mg/g). The Cr(VI) and Pb(II) removal process of CS@TMT was better fitted by the pseudo-second-order kinetic model and Langmuir model, and the adsorption process was spontaneous, endothermic and disordered. Under the reduction mechanism, 69.32% of Cr(VI) was reduced to Cr(III) by -SH and –OH group on CS@TMT.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Hongru Zhao,Chengqiang Hu,Na Sui,Xinyu Liu,Junlian Wang
Zr/Hf separation has always been a great challenge. Although the methyl isobutyl ketone (MIBK)-thiocyanic acid (HSCN) extraction system selectively extracts Hf over Zr and can realize Zr/Hf separation, it has the following disadvantages: it has a strong smell; it is easy to burn; and there is large loss of extractant during extraction due to the low boiling point (115.7 °C), low flashing point (13 °C), and high solubility of MIBK in water (1.7 % at 25 °C). In this study, Zr/Hf separation performance by MIBK homologues — of which one side of C = O is iso pentyl, iso hexyl, n -pentyl, n -hexyl or n -heptyl, while the other side are all methyl — were studied and compared with MIBK. Effects of HSCN pre-extraction, HCl concentration, NH 4 SCN concentration on Zr, Hf extraction and separation performance were investigated. Relationships between methyl alkyl ketone structure and their Zr/Hf separation performance were revealed. A new Zr/Hf separation system MNPK (methyl n -pentyl ketone)-HSCN was found. MNPK has a higher boiling point (151 °C), a higher flashing point (47 °C) and a lower solubility in water (0.43 % at 20 °C) than MIBK. Compared to MIBK, it is safer, more friendly operation environments and less extractant loss during extraction. MNPK has as fast extraction kinetics as MIBK. It also has good anti-emulsification performance. Its maximum β Hf/Zr can reach to 12.61. The loaded Zr and Hf in MNPK have very similar stripping behaviors with those loaded in MIBK by HCl and H 2 SO 4 solutions, respectively. MNPK is the most possible candidate to replace MIBK from HSCN media in Zr/Hf separation industry.
研究领域:
化学工程
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材料科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Min Zhang,Jin Wang,Xinyuan Zhan,Wusong Xu,Maolin He,Ding Ma,Zhengbo Yue
Thiocyanate (SCN − ), a non-volatile inorganic pollutant, is commonly found in various types of industrial wastewater, which is resistant to hydrolysis and has the potential to be toxic to organisms. Premagnetized iron-copper-carbon ternary micro-electrolytic filler (pre-Fe/Cu/C) was prepared to degrade SCN − . Pre-Fe/Cu/C exhibited the most significant enhancement effect on SCN − removal when magnetized for 5 min with an intensity of 100 mT, and the SCN − removal rate was the highest at an initial pH of 3.0 and an aeration rate of 1.6 L/min. The electrochemical corrosion and electron transfer in the pre-Fe/Cu/C system were confirmed through SEM, XPS , FTIR , XRD , and electrochemical tests. This resulted in the formation of more corrosion products and multiple cycles of Fe 2+ /Fe 3+ and Cu 0 /Cu + /Cu 2+ . Additionally, density functional theory (DFT) calculations and electron paramagnetic resonance (EPR) were utilized to illustrate the oxygen adsorption properties of the materials and the participation of reactive oxygen species ( 1 O 2 , ·O 2 − , and ·OH) in SCN − removal. The degradation products of SCN − were identified as SO 4 2− , HCO 3 − , NH 4 + , and N 2 . This study introduced the use of permanent magnets for the first time to enhance Fe/Cu/C ternary micro-electrolytic fillers, offering a cost-effective, versatile, and stable approach that effectively effectively enhanced the degradation of SCN − .
研究领域:
环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 40
·17296-17301
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IF 3.288
Tao Lei,Ting Huang,Tianze Wang,Peng Yu,Taiping Qing,Beixi Nie
Thiocyanate (SCN−) is a metabolite of cyanide detoxification, which is considered to be a biomarker of environmental tobacco smoke exposure. Herein, a simple, fast and label-free fluorescent sensor was developed to determine SCN− based on the in situ formation of DNA-templated fluorescent copper nanoclusters (CuNCs). Without SCN−, the Cu(II) ions in buffer were reduced by ascorbic acid and then accumulated on the DNA template, showing a high fluorescence. However, in the presence of SCN−, the Cu(II) ions reacted with thiocyanate and then the remaining unreacted Cu(II) ions were reduced by ascorbic acid, producing a small amount of the CuNCs and a weak fluorescence. Several important parameters such as the concentrations of DNA, Cu(II) ions and ascorbic acid were optimized. Under the optimized conditions, the linear range was 10–100 μM and the limit of detection (LOD) was 4.94 μM for thiocyanate assay. Due to the strong chelation between SCN− and the Cu(II) ions, high selectivity was achieved for SCN− detection by this sensor, with practical applications in real water and artificial saliva. The operation process is simple and the signal response of this method to thiocyanate is fast, within a few minutes. Therefore, the developed assay has great potential to determine thiocyanate for the assessment of water safety and human health.
研究领域:
分析化学
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生物医学工程
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环境科学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Long Jiang,Yaojie Liu,Xu Meng,Mo Xian,Chao Xu
Removal of toxic heavy metals needs to be assured from drug substances and catalyst residues from waste streams at the ppm level which has sometimes proved difficult, causing irreversible harm to human health and environment. Herein, on basis of binding evaluation between isothiocyanate derivatives in Pd(MeCN) 2 Cl 2 model solution, the corresponding functional materials were prepared by efficiently nucleophilic substitution and isomerization reaction on polystyrene framework. The isomerization from thiocyanide to isothiocyanate gifted SCN-2-NCS excellent Pd 2+ adsorption capacity which could tolerate various composite solvent systems and was generally superior than the widely-used isothiourea modified material PSTU. Systematic thermodynamics and kinetics study indicated the complexation with Pd 2+ was kinetically hindered and the increase of temperature contributed to the adsorption process as the maximum adsorption capacity was evaluated as 4.995 mmol·g −1 . The breakthrough point for SCN-2-NCS was 385–390 BV in Pd(MeCN) 2 Cl 2 solution (1.000 mmol·L -1 ) and reached to 126–130 BV in Pd-catalyzed debenzylation waste stream (5.433 mmol·L -1 ). Subsequently, computational chemistry certified an electrophilic addition reaction occurred to generate a five-member Pd 2+ complex in adsorption process which could be converted to N-substituted thiocarboxylic acid in acid condition, and FTIR, NMR and XPS analysis were further employed to probe the proposed adsorption mechanism.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
Journal of the Iranian Chemical Society
·
2025
·
IF 2.3
Tan Yan,Hu Jing,Tang Dongjin,Yu Peng
Thiocyanate (SCN − ) is an anionic contaminant and is a biomarker of environmental cyanide exposure. Therefore, it is particularly necessary to develop a simple, fast and sensitive method for SCN − assay. Herein, a fluorescent assay method was developed to determine SCN − based on Cu 2+ -triggered oxidation of o-phenylenediamine (OPD). Without SCN − , OPD was oxidized by Cu 2+ and the resultant product (i.e., OPD oxide) exhibited a high fluorescence. However, in the presence of SCN − , Cu 2+ reacted with SCN − ; and thus, the oxidation ability of Cu 2+ toward OPD was reduced, resulting in a weak fluorescence. Several important parameters such as the concentrations of Cu 2+ and OPD, pH and the reaction time were optimized. Under the optimized conditions, the linear range was 5–80 μM and the limit of detection was 5 μM for SCN − assay. In addition, the high selectivity and the satisfactory recoveries in tap water and artificial saliva were achieved for SCN − detection due to the strong chelation between SCN − and Cu 2+ . The whole detection process was simple and fast without using complex nanoparticles. More importantly, the developed assay method will have promising applications in monitoring of SCN − for the assessment of water safety and human health. Graphical abstract
研究领域:
分析化学
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环境科学
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生物医学
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产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2025
·
IF 8.4
Zhuocheng Wei,Bin Zhang,Rui Xu,Haoling Li,Acong Chen,Chaohai Wei,Haizhen Wu
Thiocyanate (SCN − ) is a highly toxic reducing inorganic compound commonly found in various nitrogen-rich wastewater and is also a promising electron donor for mixotrophic denitrification. However, its extent of involvement in mixotrophic denitrification under conditions of carbon limitation or excess remains unclear. In this study, five reactors were constructed to investigate the participation and microbial mechanisms of SCN − in mixotrophic denitrification under high C/N and low C/N conditions. The results showed that under low C/N conditions, SCN⁻ synergizes with organic electron donors to enhance denitrification, with mixotrophic systems exhibiting higher electron utilization efficiency and achieving 1.12 times the TN removal rate of autotrophic and heterotrophic systems combined. Under high C/N conditions, an ample supply of electron donors achieve 100% nitrate removal; however, SCN⁻ also participates in denitrification, competing with organic electron donors for NO 3 − -N, which leads to the waste of organic matter. Additionally, high-throughput sequencing revealed that under low C/N mixotrophic conditions, SCN⁻ effectively promoted the growth of SCN⁻-degrading microorganisms such as Thiobacillus , with its abundance increasing from 0% to 8.86%, approximately 1.85 times higher than under autotrophic conditions. This enhancement strengthened the sulfur and nitrogen metabolic capabilities of the microbial community, enabling the system to utilize SCN − more fully for denitrification. This study provides novel insights for reducing the addition of external organic matter to nitrogen-rich wastewater containing SCN − , offering theoretical and technical support for energy-saving and emission reduction in denitrification processes of actual industrial wastewater treatment.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Mintong Tian,Zehua Li,Purui Yu,Meng Zhou,Mingming Li,Guanghao Li,Qipeng Yuan
Glucosinolates (GLs) serve as crucial precursors for bioactive isothiocyanates (ITCs). As a GL, glucoraphenin (GRE) exhibits significant potential for diverse applications. Despite being deemed stable, massive GRE decomposed during cooking and processing. To further enhance the application of GRE, the decomposition mechanisms were investigated by initiating the decomposition reactions through manipulation of concentration, temperature, pH value, solvents, and reducing agents. The decomposition products were identified using NMR and LC-MS to further elucidate the decomposition mechanisms. The solvolysis initiated the decomposition, with the decomposition products being determined by the selective removal of either glucose or thioglucose, which was influenced by exogenous factors. The removal of thioglucose disabled the formation of ITCs, and nitrile was generated through a redox mechanism involving the removal of HS-OH. These mechanisms helped elucidate the effects of various factors on GRE decomposition and provided insights for effective utilization of GRE during the cooking or processing.
研究领域:
植物化学
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食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Gan Wu,Qi Cheng,Zhaoyang Ding,Jing Xie
Biogenic amines are critical indicators for the quality and safety of aquatic products, and biogenic amine poisoning could threaten human health. Therefore, it is crucial to develop easily operated and efficient analytical sensors for strict tyramine detection. To address this need, ratiometric fluorescent sensors have been developed for the detection of tyramine. F8BT-RBI polymer dots and PVK-FITC polymer dots were prepared by the semiconductor polymers Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (F8BT) and Poly (9-vinylcarbazole) (PVK), combined with rhodamine B isothiocyanate (RBI) and fluorescein isothiocyanate (FITC) through reprecipitation methods. The ratiometric fluorescent sensors rely on the changes in fluorescence intensity in dual channels caused by the intramolecular charge transfer (ICT) resulting from the reaction between isothiocyanate dyes and tyramine. This approach enables accurate detection of tyramine content within specific linear ranges. For instance, PF8BT-RBI covers a range of 2.74–20.6 mg L −1 with a limit of detection (LOD) of 0.14 mg L −1 , while PPVK-FITC covers 20.6–61.7 mg L −1 with an LOD of 0.59 mg L −1 . PF8BT-RBI and PPVK-FITC had potential for fluorescence detection of tyramine, and were applied in the detection of tyramine in aquaculture water and tuna. By utilizing Pdots and isothiocyanate dyes, these sensors can meet the demand for ensuring the freshness and safety of aquatic products.
研究领域:
食品安全
·
分析化学
·
生物传感
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Hao-Hua Deng,Chun-Lin Wu,Ai-Lin Liu,Guang-Wen Li,Wei Chen,Xin-Hua Lin
A convenient colorimetric sensor based on anti-aggregation of citrate-capped gold nanoparticles (AuNPs) is demonstrated for thiocyanate (SCN − ) sensing. The citrate-capped AuNPs aggregated readily in the presence of sulfuric acid , leading to a color change from red to blue. This particle aggregation could be suppressed by SCN − , which had strong protective effect on the gold colloid . The thiocyanate recognition suffers insignificant interference from most of common ions. With this strategy, 1 μM of SCN − can be recognized within 5 min by naked-eye observation. This simple, selective, and sensitive approach can be potentially applied for SCN − assays in environmental aqueous samples.
研究领域:
分析化学
·
纳米科技
·
环境科学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2018
·
IF 4.358
Guo Lin,Shixing Wang,Libo Zhang,Tu Hu,Song Cheng,Likang Fu,Chao Xiong
A novel trithiocyanuric acid-modified corn bract (TCA-CCB) was prepared, and its removal properties for Hg 2+ were investigated. TCA-CCB showed a remarkable absorbability for Hg 2+ in mixed ion solutions. Adsorption kinetics experiments indicated that the removal of Hg 2+ on TCA-CCB was quick, with a removal rate of 99.07% within 5 min. In addition, the removal rate of Hg 2+ exceeded 98% over all pH conditions. The adsorption process can be best described by pseudo-second-order kinetic and Hill isotherm models. The saturated adsorption capacity of TCA-CCB for Hg 2+ was 390 mg/g. The TCA-CCB could efficiently adsorb Hg 2+ from the simulated wastewater and reduce the Hg 2+ concentration from 10 ppm to 12.35 ppb, which was lower than the greatest allowable value of 50 ppb and satisfied the emission standards required by the Chinese government. Moreover, the removal rate of Hg 2+ was beyond 99% after three cycles. The results of the zeta potential and X-ray photoelectron spectroscopy (XPS) implied that the chelation and ion exchange between amino/thiol groups and Hg 2+ played a significant role in the improvement of the adsorption properties. The corn bract modified by trithiocyanuric acid exhibits apparent advantages in the removal of Hg 2+ from ppm to ppb due to its high selectivity , adsorption capacity and stability.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）