Analytical Methods
·
2021
·Vol. 13
·期 26
·2974-2980
·
IF 2.896
Xinze Wu,Yifei Cao,Bin Sun,Huaixia Chen,Xueping Dang,Xiaolan Liu,Youhong Ai
In this study, magnetic Fe3O4@SiO2@β-cyclodextrin copolymerized microparticles were synthesized and applied for the extraction of methyl parathion and fenthion in lettuce samples followed by HPLC-UV detection. The magnetic β-cyclodextrin copolymerized microparticles were prepared by dispersion polymerization with acryloyl β-cyclodextrin as the functional monomer and ethylene glycol dimethyacrylate as the crosslinker. The composite magnetic microparticles were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, magnetic measurement, and thermogravimetric analysis, and used as the adsorbent of magnetic solid-phase extraction (MSPE) for methyl parathion and fenthion. The extraction conditions including sample pH and ionic strength, desorption solvent type and volume, and adsorption and desorption times were optimized. Under the optimal extraction conditions, an MSPE-HPLC-UV method was developed for the detection of methyl parathion and fenthion in lettuce. Wide linear ranges of 1.0–200 μg kg−1 (R2 = 0.9998) for methyl parathion and 1.5–200 μg kg−1 (R2 = 0.9978) for fenthion were obtained and the limits of detection were 0.3 μg kg−1 for methyl parathion and 0.5 μg kg−1 for fenthion in lettuce, respectively. The proposed method was applied for the determination of methyl parathion and fenthion in lettuce with satisfactory recoveries between 89.2–101.2%, and relative standard deviations were less than 9.1%. Thus, the MSPE-HPLC-UV method has high accuracy and sensitivity for the analysis of methyl parathion and fenthion in lettuce samples.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CHINESE CHEMICAL LETTERS
·
2017
·
IF 1.932
Wei Song,Hai-Juan Zhang,Ying-Hua Liu,Cui-Ling Ren,Hong-Li Chen
A new facile fluorescence probing strategy, which was based on N -doped carbon dots (NCDs) and methyl parathion hydrolase (MPH), was developed for the determination of parathion-methyl (PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 μmol/L, with a detection limit of 0.338 μmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully. Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p -nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.
研究领域:
环境科学
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分析化学
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生物化学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lijun Wang,Jiangchuan Tang,Shanshan Ren,Haixia Hu,Xiao Yang,Xianggui Chen
Although many colorimetric methods have been developed for on-site pesticide detection, most exhibit low sensitivity and limited resistance to interference. To address these issues, this study developed a novel dual-mode colorimetric-ratiometric fluorescence method for isocarbophos (ICP) detection using sulfur and nitrogen co-doped carbon nanodots (S, N-CDs) to inhibit catalytic Fe 3 O 4 @Cu-BTC nanozyme activity. The synthesized Fe 3 O 4 @Cu-BTC nanozymes with higher peroxidase-like activity were inhibited by S, N-CDs. The inhibitory mechanism was deeply investigated and was ascribed to (1) the restriction of the reaction between Fe 3 O 4 @Cu-BTC and hydrogen peroxide (H 2 O 2 ), which decreased the ·OH production, and (2) the ·OH scavenging ability of the S, N-CDs, forming the basis of the dual-mode method. The Fe 3 O 4 @Cu-BTC/Apt-complementary strand (CS)/S, N-CDs hybridization complexes demonstrated minimal to no peroxidase-like activity, preventing H 2 O 2 from oxidizing o -phenylenediamine (OPD) to produce yellow fluorescent 2,3-diaminophenazine (DAP). Meanwhile, the detection system demonstrated a low fluorescence intensity ratio (I 575 /I 465 ) at an excitation wavelength of 390 nm. However, the presence of ICP caused the CS/S, N-CDs to separate from the Fe 3 O 4 @Cu-BTC/Apt due to the high affinity between Apt and ICP. Then, the Fe 3 O 4 @Cu-BTC/Apt-ICP complexes recovered peroxidase-like activity, which promoted DAP production, accompanied by a pronounced fluorescence intensity ratio (I 575 /I 465 ). In optimum conditions, the detection limits (LODs) were 2.79 ng/mL (colorimetric) and 0.26 ng/mL (ratiometric fluorescence), respectively. The feasibility of the proposed method was verified via three spiked samples, yielding recovery rates of between 86.08 % and 112.64 %, indicating the significant potential for the highly sensitive, accurate on-site detection of ICP in actual samples.
研究领域:
分析化学
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环境科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Catalysts
·
2024
·Vol. 14
·期 6
·346
·
IF 3.8
Jingfei Luan,Jun Li,Ye Yao,Bowen Niu,Liang Hao,Yichun Wang,Zhe Li
A new catalyst, Sm2NdSbO7, was synthesized for the first time by solid-phase sintering. The study utilized X-ray diffraction, transmission electron microscope energy dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy to examine the structural characteristics of monocrystal BiDyO3, monocrystal Sm2NdSbO7and Sm2NdSbO7/BiDyO3heterojunction photocatalysts (SBHP) prepared by solid-phase sintering. The Sm2NdSbO7photocatalyst owned a pyrochlorite structure, belonged to the face-centered cubic crystal system, possessed a space group of Fd3m and a bandgap width of 2.750 eV. After 145 min of visible light irradiation (145-VLIRD), the removal rate (RMR) of methyl parathion (MP) or total organic carbon of SBHP was 100% or 97.58%, respectively. After 145-VLIRD, the photocatalytic degradation rates of SBHP to MP were 1.13 times, 1.20 times, and 2.43 times higher than those of the Sm2NdSbO7photocatalyst, the BiDyO3photocatalyst, and the nitrogen-doped TiO2catalyst, respectively. The experimental results showed that SBHP had good photocatalytic activity. After four cycles of cyclic degradation experiments with SBHP, the elimination rates of MP were 98.76%, 97.44%, 96.32%, and 95.72%, respectively. The results showed that SBHP had good stability. Finally, the possible degradation pathways and degradation mechanisms of MP were speculated. In this study, we successfully developed a high-efficiency heterojunction catalyst which responded to visible light and possessed significant photocatalytic activity. The catalyst could be used in photocatalytic reaction system for eliminating the harmful organic pollutants from wastewater.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Jingna Cui,Yimu Wei,Jiangong Jiang,Shouchun Xiao,Xueke Liu,Zhiqiang Zhou,Donghui Liu,Peng Wang
The bioaccumulation, metabolism, tissue-specific distribution and toxicity of the widely used organophosphorous pesticide malathion to zebrafish were investigated on an enantiomeric level for evaluating the environmental risks. The metabolites were also monitored and evaluated. Malathion was metabolized by zebrafish very fast with the half-life of 0.12 d and showed a middle accumulation capacity in zebrafish with bioaccumulation factor (BCF) of 12.9 after a 15-d exposure. Brain could enrich higher concentration of malathion than other tissues. The metabolites malaoxon, malathion/malaoxon monocarboxylic acid (DMA), malathion/malaoxon dicarboxylic acid (DCA), dimethylthiophosphate (DMTP) and dimethyldithiophosphate (DMDTP) were found, in which DMTP and DCA were in higher level, indicating the metabolism was mainly induced by carboxylesterase degradation. The accumulation of malathion and malaoxon was stereoselective in zebrafish tissues, exhibiting S-enantiomer preferentially enriched. The acute toxicity test showed rac-malathion was low toxic to zebrafish, which was 1.2 and 1.6 folds more toxic than S-malathion and R-malathion respectively. Malaoxon was highly toxic to zebrafish and approximately 32 times more toxic than malathion. The toxicity of other metabolites was lower than malathion. Malathion could cause an apparent developmental toxicity to zebrafish embryo, including bradycardia, hatchability reduction and deformity, and abnormal movement patterns in zebrafish larva. Chronic toxicity indicated that malathion and malaoxon induced oxidative damage and neurotoxicity in the liver, brain and gill of zebrafish, and malaoxon exhibited a relatively high injury to the zebrafish brain. The results can provide information for the comprehensive assessment of the potential risk of malathion to aquatic organisms and highlight the necessity of consideration of stereoselectivity and metabolites when systemically evaluating pesticides.
研究领域:
环境科学
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毒理学
·
水产生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF STORED PRODUCTS RESEARCH
·
2022
·
IF 2.831
Zhengyan Wang,Wenfang Wang,Yujie Lu
Stored grain pests have developed widespread resistance to currently available grain protectants. Although symbiotic microbes played an important role in insecticide resistance of insect hosts, it has not been reported in stored grain pests. In this study, five gut bacteria were excised from adult Sitophilus oryzae (L.) (Coleoptera: Curculionidae), Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae), and Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). These bacteria can degrade malathion , pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro . When treated with 0.5–10 mg/kg of malathion, pirimiphos-methyl and 0.3–0.75 mg/kg of deltamethrin, gnotobiotic reinoculation of a given bacterial strain significantly enhanced survival rates of its hosts. The significance of gut bacteria in host insecticide tolerance was insecticide dosage-dependent. Furthermore, the in vitro insecticide biodegradability of gut bacteria was not completely consistent with their in vivo performance in host insecticide resistance. This suggests that there may be other physiological processes responsible for host insecticide tolerance mediated by symbiotic bacteria except for direct degradation of insecticides. These results provide insight into insecticide resistance management of stored grain pests and further study of the association between gut bacteria and insecticide resistance.
研究领域:
昆虫学
·
微生物学
·
农药科学
·
农业科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-12
·
IF 6.232
Liu Lixia,Yang Minli,He Muyi,Liu Tong,Chen Fengming,Li Yinlong,Feng Xuesong,Zhang Yuan,Zhang Feng
A novel magnetic dual-template molecularly imprinted polymer (DMIP) was prepared with methyl-parathion and quinalphos as templates. For comparison, a series of single-template polymers with only methyl-parathion (MPMIP) or quinalphos (QPMIP) as template as well as a non-imprinted polymer (NIP) in the absence of the template, were synthesized using the same procedure of DMIP. The obtained MIPs were characterized by scanning electron microscopy(SEM), Fourier transform infrared (FT-IR) spectroscopy, vibrating sample magnetometer (VSM), and X-ray diffraction (XRD). The properties including kinetic effect, thermodynamic effect, selectivity, and reusability of MIPs were investigated . Only DMIP possessed high affinity and good recognition for all twelve OPPs including quinalphos, isazophos, chlorpyrifos-methyl, chlorpyrifos, methidathion, triazophos, profenofos, fenthion, fenitrothion, methyl-parathion, parathion, and paraoxon in comparison to MPMIP, QPMIP, or NIP. Moreover, DMIP was used as magnetic solid phase extraction (MSPE) sorbent for the pre-concentration of twelve OPPs in cabbage samples. The developed DMIP-MSPE-GC-MS method showed high sensitivity, low LODs (1.62–13.9 ng/g), fast adsorption equilibrium (10 min), and acceptable spiked recoveries (81.5–113.4%) with relative standard deviations (RSD) in the range 0.05–7.0% ( n = 3). The calibration plots were linear in the range 10–800 ng/mL with coefficients of determination ( R 2 ) better 0.99 for all twelve compounds. These results suggest that the DMIP is applicable for rapid determination and high throughput analysis of multi-pesticide residues.
Graphical abstract
研究领域:
分析化学
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环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
He Mei,Jingcheng Huang,Guixin Wang,Kun Chen,Huili Wang,Xuedong Wang,Wenwei Wang
Herein, we engineered a novel pH-responsive ratiometric assay by virtue of the composite system of Ce 3+ -enhanced glutathione-encapsulated copper nanoclusters (GSH-CuNCs-Ce 3+ ) and N-doped carbon quantum dots (N-CQDs). The GSH-CuNCs-Ce 3+ and N-CQDs were fabricated using one-pot chemical reduction and hydrothermal approaches, respectively, and their morphologies as well as physical and chemical properties were testified in detail by a series of characterization techniques. At 350 nm excitation wavelength, the GSH-CuNCs-Ce 3+ /N-CQDs system (GCC-NC) featured the 440/650 nm dual-emitting property. The ratios of fluorescence intensities (F 650 /F 440 ) demonstrated a strong pH-dependence from 3.0 to 5.0 with a coefficient of determination of 0.9982. Consequently, the GCC-NC system was feasible for constructing a pH-responsive dual-emitting fluoroprobe. Based on the catalytic effect of acetylcholinesterase on acetylcholine to yield acetic acid and further trigger varying solution pH, the as-constructed GCC-NC fluoroprobe was satisfactorily applied for detecting malathion . Under optimized conditions, the newly developed GCC-NC fluoroprobe supplied a wider linear range (0.25–200 μM), lower detection limit (0.075 μM), satisfactory recoveries (96.3–106.2%) and higher precision for malathion in several kinds of fruit juice samples. The fortified experiments by malathion’s structural analogues and metal ions provided compelling evidence that this fluoroprobe had strong anti-interference capacity and high specificity for malathion. These findings render us to believe that the as-constructed pH-responsive dual-emitting fluoroprobe holds great promise in trace malathion assay in food matrices.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Linghui Huang,Yue Tang,Jianxun Han,Xiaojuan Niu,Xin Lin,Yuangen Wu
Malathion causes a serious threat to human health due to its widespread use in the environment. Herein, a novel and stable smartphone-integrated colorimetric biosensor for malathion detection is firstly established based on aptamer-enhanced laccase-mimicking activity. The results indicate that the M17-F aptamer can increase the affinity of Ag 2 O nanoparticles to the substrate 2,4-dichlorophenol and enhance their laccase-mimicking activity. Thus, abundant semiquinone radicals are produced in the catalytic system, which are combined with chromogenic agent to generate dark red products. The corresponding RGB values for the colour change of the solution can be easily obtained using smartphones, which is used for the rapid detection of malathion. The established biosensor for malathion has a limit of detection as low as 5.85 nmol·L −1 , and displays good selectivity for other competitive pesticides. Moreover, further studies have verified the applicability of the biosensor in actual samples, indicating that it may have the potential for application in malathion detection in food.
研究领域:
生物化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2024
·Vol. 14
·期 2
·144
·
IF 3.8
Jingfei Luan,Yichun Wang,Ye Yao,Liang Hao,Jun Li,Yu Cao
Eu2SmSbO7and ZnBiEuO4were synthesized for the first time using the hydrothermal method. Eu2SmSbO7/ZnBiEuO4heterojunction photocatalyst (EZHP) was synthesized for the first time using the solvothermal method. The crystal cell parameter of Eu2SmSbO7was 10.5547 Å. The band gap width of Eu2SmSbO7was measured and found to be 2.881 eV. The band gap width of ZnBiEuO4was measured and found to be 2.571 eV. EZHP efficiently degraded the pesticide chlorpyrifos under visible light irradiation (VLID). After VLID of 160 min, the conversion rate of the chlorpyrifos concentration reached 100%, while the conversion rate of the total organic carbon (TOC) concentration was 98.02% using EZHP. After VLID of 160 min, the photocatalytic degradation conversion rates of chlorpyrifos using EZHP were 1.13 times, 1.19 times, and 2.84 times those using Eu2SmSbO7, ZnBiEuO4, and nitrogen-doped titanium dioxide (N-doped TiO2), respectively. The photocatalytic activity could be ranked as follows: EZHP > Eu2SmSbO7> ZnBiEuO4> N-doped TiO2. The conversion rates of chlorpyrifos were 98.16%, 97.03%, 96.03%, and 95.06% for four cycles of experiments after VLID of 160 min using EZHP. This indicated that EZHP was stable and could be reused. In addition, the experiments with the addition of capture agents demonstrated that the oxidation removal ability of three oxidation free radicals for degrading chlorpyrifos obeyed the following order: hydroxyl radical > superoxide anion > holes. This study examined the intermediates of chlorpyrifos during the photocatalytic degradation of chlorpyrifos, and a degradation path was proposed, at the same time, the degradation mechanism of chlorpyrifos was revealed. This study provides a scientific basis for the development of efficient heterojunction photocatalysts.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Xue Cao,Chenzhi Yan,Xuerui Yang,Lei Zhou,Wenjun Zou,Guangli Xiu
Contamination of the environment by toxic pesticides has become of great concern in agricultural countries. Chlorpyrifos (CP) is among the pesticides most commonly detected in the environment owing to its wide agricultural applications. The aim of this study was to compare potential changes in the toxicity of CP after irradiation. To this end, photolysis of CP was conducted under simulated sunlight, and neurotoxicity assessment was carried out at CP of 20 and 50 μg L–1 and its corresponding irradiated mixture solutions which contain a mixture of identified intermediates using the nematode, Caenorhabditis elegans as a model organism. Photodegradation of 20 μg L–1 CP for 1 h produced no obvious reduction of physiological damage, and more serious effects on animal movement were detected after exposure of the animals to a solution of 50 μg L–1 for 1 h irradiation compared with unirradiated solution. GABAergic and cholinergic neurons were selectively vulnerable to CP exposure, and maximal neuropathological alterations were observed after 1 h irradiation of the CP solutions in coherence with the behavioral impairment. The generation of photoproducts was considered to be responsible for the enhanced disturbance on those biological processes. This work provided useful information on the toxicological assessments of chemicals that were produced during the environmental transformation of pesticides.
研究领域:
环境科学
·
毒理学
·
生物学
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DOI
PubMed
高影响因子
Journal of Earth Science
·
2019
·Vol. 30
·期 4
·861-869
·
IF 1.784
Zhang Caixiang,Liao Xiaoping,Lü You,Nan Chao
The ligand-stabilized soluble Mn(III) recognized as active intermediate can potentially mediate the attenuation of contaminants. In this study, the abiotic degradation behaviors of methyl parathion in the ligand stabilized Mn(III)-sulfite system were investigated. The results showed that the yield of soluble Mn(III) produced from the redox reaction of MnO 2 and oxalic acid was dependent linearly on the dosage of MnO 2 and caused the decomposition of methyl parathion up to 50.1% in Mn(III)-sulfite system after 30 minutes. The fitted pseudo-first-order reaction constants of methyl parathion degradation increased with the increasing of the amount of produced Mn(III) but was not effected linearly by the addition of sulfite. Other ligands, including pyrophosphate and oxalic acid, acted as effective complexing agents to stabilize soluble Mn(III), and exhibited competitive effect on methyl parathion degradation with sulfite. The formation of Mn(III)-sulfite complexes is the critical step in the system to produce abundant reactive oxygen species identified as SO 3 • - to facilitate methyl parathion degradation. The hydrolysis and oxidation of methyl parathion were acknowledged as two primary transformation mechanisms in Mn(III)-sulfite system. These findings indicate that naturally ligands-stabilized soluble Mn(III) can be generated and could oxidatively decompose organophosphate pesticides such as methyl parathion.
研究领域:
环境科学
·
化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）