SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Pengjuan Ni,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Wei Chen
Hemin, the most common biological iron form, serves many biological functions. However, studies on hemin detection are scarce. As an unusual natural peroxide, artemisinin is quite stable in the presence of common species. While, it can decompose to generate superoxide radicals in the presence of hemin. And the as-obtained superoxide radicals can effectively oxidize thiamine to form fluorescent thiochrome. Inspired by this, a simple and novel fluorescent assay for hemin detection based on artemisin-thiamine is developed. Based on the linear relationship between the fluorescence intensity and concentration of hemin, the developed artemisinin-thiamine assay can be utilized for sensitive detection of hemin in the concentration range of 2.0–300.0 nM with a detection limit of 0.68 nM. Moreover, this assay shows high selectivity over other cations and biomolecules. Besides, hemin can be detected in serum samples with satisfactory results, which demonstrates its great potential for real sample analysis. The designed assay is free of any fluorescent nanomaterials or probes by just mixing these reagents together, and all the detection processes is performed within 20 min. Therefore, it can offer a simple, highly sensitive and selective sensing platform for fluorescence detection of hemin.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Chen Yunhai,Zhu Xuecheng,Liu Huilin,Sun Baoguo
Room temperature phosphorescent carbon dots ( NC CDs@SiO 2 ) were obtained by encapsulating hydrothermally synthesized CDs in a dense Si-O network structure after high-temperature calcination using silica as the matrix. This can avoid the quenching effect of dissolved oxygen in water and has a phosphorescence lifetime of up to 2.41 s. Using the phosphorescence property of NC CDs@SiO 2 , a phosphorescence quenching sensor was developed for the sensitive and selective detection of thiram with the assistance of Cu 2+ . Cu 2+ -thiram complexes led to a rapid phosphorescence quenching of NC CDs@SiO 2 within 30 s through the inner filter effect. The linear range of phosphorescence for thiram was 0.5–100 µM with a detection limit of 0.121 µM. The proposed method was able to detect thiram in real samples and was validated by high-performance liquid chromatography (HPLC) confirming the potential of this phosphorescence sensing method for thiram detection. This work opens up a new avenue for the detection of thiram residues in fruits and vegetables and also provides a new idea for the design of a rapid detection platform using other room temperature phosphorescent materials. Graphical Abstract
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-5
·
IF 5.705
Ni Pengjuan,Chen Chuanxia,Jiang Yuanyuan,Zhao Zhenlu,Lu Yizhong
A fluorometric assay is described for sulfide ions determination. It is based on the finding that the oxidation of the non-fluorescent substrate thiamine (TH) by Cu(II) in basic solution to form fluorescent thiochrome is inhibited by sulfide ions. This results in a decrease in fluorescence intensity which is proportional to the concentration of sulfide ions. Under the optimized conditions, the decrease in fluorescence, best measured at excitation/emission wavelengths of 370/440 nm, decreases linearly in the 0.03 to 2.5 μM sulfide ions concentration range. The detection limit is 20 nM. The method shows excellent selectivity over many potentially interfering ions and has been successfully applied to the determination of sulfide ions in spiked tap water, lake water and the synthetic wastewater samples. The method is time-saving and environmentally friendly, and in our perception shows a great potential in water quality inspection and environmental monitoring.
A fluorescent assay for sulfide ions detection is proposed based on its inhibitory effect on the oxidation of thiamine by Cu(II) ions.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
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2018
·Vol. 23
·期 6
·1442
·
IF 3.098
Wenxiao Jiao,Xiangxin Li,Handong Zhao,Jiankang Cao,Weibo Jiang
Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE–Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination ofPenicillium expansum, one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP againstP. expansum. Results demonstrated that BanTLP exhibited antifungal activity in a wide pH range (4.0–10.0) at 20–50 °C. Propidium iodide (PI) influx and potassium release confirmed that BanTLP induced membrane disruption of the test pathogen, increasing the membrane permeability and disintegration of the cell. This led to cell death, as evidenced by the assays of thiobarbituric acid-reactive species (TBARS) content, the production of reactive oxygen species (ROS), and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence integrity. Ultrastructural alterations inP. expansumconidia after BanTLP treatment revealed severe damage to the cell wall. These results suggest that BanTLP purified from banana exerts antifungal activity againstP. expansumby inducing plasma membrane disturbance and cell wall disorganization.Keywords:banana;thaumatin-like protein;antifungal activity;Penicillium expansum;mechanisms of action
研究领域:
植物病理学
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食品科学
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 18
·4442-4447
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IF 3.864
Pengjuan Ni,Dafeng Jiang,Chuanxia Chen,Yuanyuan Jiang,Yizhong Lu,Zhenlu Zhao
In this work, we propose a fluorescence method for the simultaneous detection of glutathione (GSH) and histidine (His) based on the Cu(II)-thiamine (Cu(II)-TH) system. It is well established that non-fluorescent thiamine (TH) can be oxidized by Cu(II) to generate fluorescent thiochrome (TC) under alkaline conditions. The introduction of GSH and His can inhibit the oxidation of TH by Cu(II) due to the strong affinity between Cu(II) and GSH or His. With this strategy, the detection limit for GSH and His is 10.5 nM and 26.4 nM, respectively. The developed method shows several obvious advantages: (1) simplicity in design and operation without the utilization of fluorescent nanomaterials or probes; (2) time-saving detection process since all the detection process is completed within 15 min; (3) cost-effectiveness by using TH as the fluorescent substrate; and (4) higher sensitivity and good selectivity. Therefore, it shows great potential in biosensing fields.
研究领域:
分析化学
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生物传感
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医学检测
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DOI
PubMed
高影响因子
Catalysts
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2024
·Vol. 14
·期 12
·911
·
IF 3.8
Shen Yu,Xue-Yong Li,Wen-Jie Si,Ye-Wang Zhang
Formolase, a thiamine pyrophosphate (TPP)-dependent enzyme, catalyzes the carboligation of three one-carbon formaldehyde molecules into one three-carbon dihydroxyacetone molecule. It has many important functions in the biosynthesis of carbon-based compounds and utilization of CO2. However, the enzyme has low activity and stability in the catalytic process, resulting in high cost in the applications. To improve the stability, formolase was immobilized onto magnetic nanoparticles, which were designed to have functional epoxy groups for covalently binding the enzyme. In the immobilization, effects of pH, temperature, and cofactor TPP on the immobilization were investigated and optimized. The results showed that the retention activity of immobilized formolase was highly related to TPP. In the presence of TPP, the specific activity of the immobilized formolase was 6.8 times higher than that without TPP. The optimal immobilization conditions were as follows: a temperature of 20 °C, a pH of 7.0, an immobilization time of 8 h, and an enzyme loading of 20 mg/g. Molecular docking was used to analyze the effect of TPP on the stabilization of the enzyme in the immobilization, which indicated that TTP could stabilize the enzyme structure during the immobilization. The stabilization effect of TPP could be a reference in the immobilization of other enzymes with TPP as the cofactor.
研究领域:
生物化学
·
酶工程
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Hongliang Tan,Qian Li,Zhengchen Zhou,Chanjiao Ma,Yonghai Song,Fugang Xu,Li Wang
Metal-organic frameworks (MOFs) with tunable structures and properties have recently been emerged as very interesting functional materials. However, the catalytic properties of MOFs as enzymatic mimics remain to be further investigated. In this work, we for the first time demonstrated the peroxidase-like activity of copper-based MOFs (HKUST-1) by employing thiamine (TH) as a peroxidase substrate. In the presence of H 2 O 2 , HKUST-1 can catalyze efficiently the conversion of non-fluorescent TH to strong fluorescent thiochrome. The catalytic activity of HKUST-1 is highly dependent on the temperature, pH and H 2 O 2 concentrations. As a peroxidase mimic, HKUST-1 not only has the features of low cost, high stability and easy preparation, but also follows Michaelis–Menten behaviors and shows stronger affinity to TH than horseradish peroxidase (HRP). Based on the peroxidase-like activity of HKUST-1, a simple and sensitive fluorescent method for TH detection has been developed. As low as 1 μM TH can be detected with a linear range from 4 to 700 μM. The detection limit for TH is about 50 fold lower than that of HRP-based fluorescent assay. The proposed method was successfully applied to detect TH in tablets and urine samples and showed a satisfactory result. We believed that the present work could improve the understanding of catalytic behaviors of MOFs as enzymatic mimics and find out a wider application in bioanalysis.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Xue Hu,Xidong Liu,Xiaodan Zhang,Haiyan Cao,Yuming Huang
By tuning the photoluminescence of alloy Cu/Ag nanoclusters (NCs) and thiamine (VB 1 ) simultaneously through MnO 2 nanowires, we report a simple and label-free ratiometric fluorescent platform for sensitively and selectively sensing of glutathione (GSH). Ag doping of Cu NCs significantly enhances the quantum yield (QY, about 2-fold enhancement), oxidation resistance to air, storage stability (60 days versus 1 day), photostability and salt stability of the resultant Cu/Ag NCs. Impressively, MnO 2 nanowires quench the fluorescence of Cu/Ag NCs, while they enhance the fluorescence of nonfluorescent VB 1 via oxidation reaction between VB 1 and dissolved oxygen to produce oxVB 1 as a nanozyme catalyst. Upon introduction of GSH as a target, the MnO 2 nanowires are reduced to Mn 2+ and lose their quenching capacity and oxidase-like activity, resulting in FL recovery of Cu/Ag NCs and fluorescent decrease of oxVB 1 . Hence, a ratiometric luminescence signal is obtained, providing a novel ratiometric fluorescent strategy for detecting GSH through target induced regulation of the catalytic activities and quenching ability of MnO 2 nanowires. The sensor exhibited a wide linear concentration range (10 nM to 70 μM) for GSH with a detection limit of 6.5 nM. The ratiometric sensor was successfully used for GSH detection in biological samples.
研究领域:
分析化学
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 1
·1-7
·
IF 6.232
Yao Zhixia,Liu Hanmeng,Liu Yaosheng,Zhang Qifang,Diao Yongxing,Sun Yujing,Li Zhuang
A fluorometric assay for histidine (His) is described. It is based on the inhibitory effect of His on nanocubes consisting of cobalt-containing Prussian Blue analog (CoFe NCbs), which have a strong oxidation effect on thiamine (THI) in the presence of NaOH. THI is nonfluorescent but the oxidized form (thiochrome; ThC) has a strong blue fluorescence, with excitation/emission maxima at 370/445 nm. His inhibits the oxidation effect of the CoFe NCbs due to the strong interaction between its imidazole side chain and the amino groups of the CoFe NCbs. This method is fast and has good sensitivity and selectivity. The lower detection limit is 14.3 nM of His, the linear range extends from 0.05 to 2.5 μM, and the relative standard deviation is calculated to be 1.5%. The method was successfully employed to quantify His in spiked serum samples.
Schematic representation of cobalt-containing Prussian Blue nanocubes (CoFe NCbs)-thiamine (THI)-based fluorometric assay for Histine (His). His inhibits the generation of thiochrome (ThC; the oxidized form of THI). The detection limit is 14.3 nM with the linear range of 0.05–2.5 μM.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yue Hu,Yu-Cong Fan,Xiao-Hui Jiang,Li-Mei Zhou,Zheng-Jun Cheng
By simultaneously regulating the photoluminescence of alloy Au/Ag nanoclusters (NCs) and thiamine (VB1) through MnO 2 nanosheets (MnO 2 NS), a novel ratiometric fluorescent probe (RF-probe) was established for sensitively and selectively monitoring proanthocyanidins (PAs). The introduction of Ag (I) ions could enhance significantly the quantum yields (QYs, 11.1%) of AuNCs based on the synthetic method of UVI (UV irradiation) combined with MWH (microwave heating). MnO 2 NS could quench the fluorescence (FL) of Au/AgNCs mainly coming from Förster resonance energy transfer (FRET), while it could act as a nanozyme catalyst for directly catalyzing the oxidation of VB1 to produce highly fluorescent oxVB1. In the presence of PAs, MnO 2 was reduced to Mn 2+ , which caused that its quenching capacity and oxidase-like activity were vanished, thus the FL of oxVB1 and Au/AgNCs was reduced and recovered. The concentration of PAs could be monitored by the RF-probe with a linear range of 0.27–22.4 μmol L −1 and corresponding limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 75.9 and 250.5 nmol L −1 . Furthermore, the RF-probe was successfully used for the determination of PAs in mineral water, PAs additive and PAs capsule with satisfactory results compared to the standard HPLC method.
研究领域:
分析化学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 12
·5990-5994
·
IF 2.562
Jinghui Zhu,Shaopu Liu,Zhongfang Liu,Yuanfang Li,Man Qiao,Xiaoli Hu
An enhanced spectrofluorimetric method for highly selective and sensitive determination of nmol L−1 of hypochlorite has been performed. In alkaline medium, ferrocyanide was specifically oxidized by hypochlorite to form ferricyanide, which further reacted with non-fluorescent thiamine, resulting in the formation of blue fluorescent thiochrome. Notably, the fluorescence intensities were positively correlated with the concentration of hypochlorite over the range of 0.02–12.5 μmol L−1. The detection limit was 6.1 nmol L−1, which was lower than for most of the recently published methods. The experimental conditions were optimized and effects of coexisting substances were evaluated and the results showed excellent priority since a certain amount of other anions, including ClO3−, BrO3−, IO3−, Cr2O72− and other acid radicals, would not interfere with the measurement.
研究领域:
分析化学
·
生物化学
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产品关联
DOI
高影响因子
CELLULOSE
·
2023
·Vol. 30
·期 7
·4437-4454
·
IF 5.7
Su Xiaowei,Tan Wei,Liu Xiaohui,Ren Yuanlin,Sun Ying
To improve the flame retardant and antibacterial properties of lyocell samples, thiamine pyrophosphate (TPP), a kind of water-soluble vitamin derivative, was selected for the modification of lyocell fabric. Vertical burning test showed that the combustion of treated lyocell fabric was greatly reduced. The thermal stability and char-forming ability of the treated lyocell fabric were significantly improved whether in air or nitrogen atmosphere. The char residue of flame retardant lyocell sample was significantly increased from 4.9 wt% of the control sample to 25.9 wt% in air atmosphere, and Raman spectroscopy confirmed the formation of graphitic carbon. The total heat release (THR) and peak heat release rate (PHRR) of the treated lyocell fabric decreased by 80.4% and 90.4%. In addition, the limiting oxygen index (LOI) values of the washed samples increased from 18.0% of the original sample to 28.3% even after 40 laundering cycles (LCs), demonstrating excellent durable flame retardancy. The flame retardant mechanism was explored by thermogravimetry-Fourier transform infrared spectrometry (TG-IR), thermogravimetry-mass spectrometry (TG-MS) and Raman spectroscopy. Furthermore, the treated lyocell sample had good antibacterial performance to E . coli and S . aureus. This research indicates that TPP is an effective and potential intumescent flame retardant and antibacterial agent, which simultaneously endows lyocell fabric with multifunctional properties.
研究领域:
材料科学
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纺织工程
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生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）