CHEMOSPHERE
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2020
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IF 5.778
Xiaoqing Li,Mingxia Zhang,Yong Li,Xiangyang Yu,Jinfang Nie
Root exudates are released by plant roots and are important carrier substances for material exchange and information transmission among plants and the rhizosphere. In the present study, the effect of dinotefuran on root exudates of Chinese cabbage ( Brassica rapa var. chinensis ) was investigated. The physiological activities revealed that dinotefuran uptake caused oxidative stress in vegetable tissues even at low dinotefuran exposure levels. The metabolic profile of plant root exudates acquired by LC-QTOF/MS was clearly changed by dinotefuran, where the numbers of both up- and down-regulated MS peaks increased with increasing dinotefuran concentration. Under dinotefuran stress, some osmotic adjustment substances (proline and betaine) and defence-related metabolites (spermidine, phenylalanine and some phenolic acids) were significantly upregulated, which may help plants adapt to adverse environmental conditions. Specifically, the contents of some phenylalanine-derived secondary metabolites increased with increasing dinotefuran concentration, which may increase the external detoxification ability of plants. Moreover, respiration metabolism was significantly affected, where some intermediates in the TCA cycle (succinic acid and malic acid) were upregulated with low-level dinotefuran exposure; however, anaerobic respiration products (lactic acid and 3-phenyllactic acid) were accumulated at high exposure levels. In addition, the release of glucosinolates was significantly inhibited in both dinotefuran treatment groups.
研究领域:
植物学
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环境科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2022
·Vol. 169
·期 8
·087506
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IF 4.386
Bolu Sun,Chengyang Gao,Lin Yang,Hongxia Shi,Lei Kan,Quhuan Ma,Xiaofeng Shi
Identification and quantification of epigoitrin (EP) in some herbs and traditional Chinese medicine (TCM) preparations are critical to pharmacokinetic study and pharmaceutical quality control due to its distinct antiviral activity. So, developing highly sensitive and selective method for detection of EP is essential for clinical treatment and drug development. In this study, a novel molecularly imprinted electrochemical sensor for detection of EP was firstly constructed. With acrylamide imprinting systems, surface imprinting on the polyaniline functionalized graphene oxide was employed to prepare molecularly imprinted polymer by electro-polymerization, which follow-up constructed afford specific binding cavities, endowing the exclusive recognition ability. Furthermore, the polyacrylamide chain is anchored to the polyaniline chain by covalent bonds, which is beneficial for raising electrochemistry signal. Under the optimized condition, the sensor demonstrates a linear wide range of 4.6 × 10−7 mol l−1 ∼ 4.6 × 10−5 mol l−1 with a correlation coefficient of 0.9953 and a low LOD of 8.21 × 10−8 mol l−1 (S/N = 3). Additionally, the sensor showed good stability, repeatability (RSD 1.52%) and selectivity. The method was applied to analyze EP in the extraction from Isatidis Radix with a recovery higher than 97.8% and RSD less than 1.81%. This work provided a novel strategy for on-site, real-time and rapid detection of indicator components from TCM.
研究领域:
中药分析
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生物传感
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药物开发
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
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2019
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IF 2.792
Yuanfeng Wu,Yuke Shen,Ye Zhu,Jothame Mupunga,Ligen Zou,Chao Liu,Shiwang Liu,Jianwei Mao
After consuming broccoli, isothiocyanates can be produced by the hydrolytic action of myrosinase from plant and/or microbiota. Using male C57BL/6 mice, the present study investigated the effects of broccoli ingestion on the myrosinase-like activity, NAD(P)H:quinone oxidoreductase 1 (NQO1) activity, diversity and composition of the gut microbiota. Compared with the control group, continuously feeding raw or hydrolysed broccoli increased the myrosinase-like activities of the colon and caecum contents, and also improved the NQO1 activity of the colon mucosa. Significant difference between the broccoli and control feeding groups were found. 16S rRNA gene sequencing indicated that broccoli ingestion profoundly affected the composition of the gut bacteria community. The correlation between the gut bacterial community composition and microbiota myrosinase-like activity was also studied. However, one type of glucosinolate, sinigrin, had no effect on these activities, indicating that broccoli component(s), other than glucosinolate, had increased the NQO1 and myrosinase-like activities.
研究领域:
营养学
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微生物学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Chong Xie,Wanmei Li,Rongguang Gao,Lihua Yan,Pei Wang,Zhenxin Gu,Runqiang Yang
The utilization of rapeseed meal in food is limited due to its abundant glucosinolates (GLs). In this study, an LC-MS/MS method for GLs determination in rapeseed meal was developed. Then, the degradation of GLs using rapeseed sprouts derived myrosinase (MYR) was investigated. Results showed that 11 kinds of GLs were identified in rapeseed meal. The LC-MS/MS method had a high linearity (R 2 greater than 0.9999), repeatability (RSD < 5%) and recovery rate (92%-102%). The optimum condition for hydrolyzing GLs in rapeseed meal was reacting for 4 h with the addition of 2236.35 U/g MYR, 9.63 μg/g ascorbic acid and 26.68 μg/g EDTA. Under this condition, more than 80% of GLs were degraded and the yields of isothiocyanates and oxazolidinone-2-thione were 859.30 μg/g and 685.59 μg/g, respectively. To conclude, this study reported a reliable method for GLs determination and an effective way to degrade GLs in rapeseed meal.
研究领域:
食品科学
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分析化学
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酶学
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产品关联
DOI
PubMed
高影响因子
Biomolecules
·
2024
·Vol. 14
·期 3
·352
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IF 4.8
Lixia He,Hanmin Jiang,Yaotong Li,Xu Zhang,Wenting Sun,Ce Liu,Zekai Zhao,Chengrong Yun,Hui Li,Chunguo Wang
Sulforaphane (SFN) is one of the hydrolysates of glucosinolates (GSLs), primarily derived fromBrassicavegetables like broccoli. In clinical therapy, SFN has been proven to display antimicrobial, anticancer, antioxidant, and anti-inflammatory properties. However, the antimicrobial effects and mechanism of SFN against plant pathogens need to be further elucidated, which limits its application in agriculture. In this study, the genetic factors involved in SFN biosynthesis in 33B. oleraceavarieties were explored. The finding showed that besides the genetic background of differentB. oleraceavarieties,myrosinaseandESPgenes play important roles in affecting SFN content. Subsequently, the molecular identification cards of these 33B. oleraceavarieties were constructed to rapidly assess their SFN biosynthetic ability. Furthermore, an optimized protocol for SFN extraction using low-cost broccoli curds was established, yielding SFN-enriched extracts (SFN-ee) containing up to 628.44 μg/g DW of SFN. The antimicrobial activity assay confirmed that SFN-ee obtained here remarkably inhibit the proliferation of nine tested microorganisms including four plant pathogens by destroying their membrane integrity. Additionally, the data demonstrated that exogenous application of SFN-ee could also induce ROS accumulation in broccoli leaves. These results indicated that SFN-ee should play a dual role in defense against plant pathogens by directly killing pathogenic cells and activating the ROS signaling pathway. These findings provide new evidence for the antimicrobial effect and mechanism of SFN against plant pathogens, and suggest that SFN-ee can be used as a natural plant antimicrobial agent for crop protection and food preservation.
研究领域:
农业
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植物科学
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分子生物学
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植物病理学
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天然产物
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
E. Hengchao,Shuting Peng,Zhiyong Zhao,Xueqin Yao,Yanmei Zhang,Xiaobei Li,Xianli Yang,Tingting Fan,Xiaoyan Zhao,Changyan Zhou
Glucosinolates (GSLs) are unique secondary metabolites in cauliflower and broccoli, which have diverse biological activities. In this study, a molecular networking combined with the equivalently quantitative ion strategy was built based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the discovery and quantification of GSLs in cauliflower and broccoli. Firstly, 19 GSLs were identified including 2 new compounds, 4-hydroxyneoglucobrassicin and gluco-4-hydroxyneoglucobrassicin. Moreover, the shared fragment ion m/z 96.0 was set as the equivalently quantitative ion to quantify GSLs. This method could use only one reference standard (or one calibration curve) to efficiently quantify all aliphatic and phenyl GSLs in cauliflower and broccoli. This method was fully validated with good linearity, recovery and sensitivity, and subsequently applied in GSLs quantification. The developed strategy could also be applied in GSLs discovery and quantification in other Brassicaceae vegetables.
研究领域:
分析化学
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食品科学
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植物科学
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植物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）