NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 43
·19974-19982
·
IF 3.3
Hao Fan,Qin Peng,Zhenxing Shen,Xuefeng He,Yiming Yang
Nitric oxide (NO), a major atmospheric pollutant and a precursor to haze, can be removed using the denitration catalyst. Mn and Ni are commonly used as denitration catalyst additives owing to their adsorption abilities for NO and ammonia (NH3). In this study, we synthesized Mn- and Ni-loaded Pt-modified sulfated cerium oxide (PtSCe) catalysts using an impregnation method and explored the effects of these elements. The results revealed that the two elements had contrasting effects on the denitration activity of the catalysts. Mn improved the water vapor and sulfur resistance of the PtSCe sample by increasing the concentration of weak acid sites, enhancing the redox ability, and increasing the content of surface-adsorbed oxygen. The elevation in oxygen vacancies, stimulated by the presence of Ce3+ and Mn3+, swiftly replenished the reactive oxygen species, thereby sustaining the high denitration efficiency of the MnPt/SCe sample. Active sites on the catalyst surface included NH3 adsorbed on Lewis acid sites, NH4+, and –NH2, along with bridging, monodentate, and bidentate nitrate groups. Regarding the catalyst reaction mechanism, we determined that the selective catalyst reduction of NH3-SCR over MnPt/SCe followed both the Eley–Rideal and Langmuir–Hinshelwood mechanisms. These findings offer crucial insights into the intricate relationship between metals, sulfates, and carrier molecules, thus providing implications for future catalyst design.
研究领域:
环境科学
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2021
·
IF 4.126
Jianzhou Wu,Kaimin Du,Jianwei Che,Shihui Zou,Liping Xiao,Hisayoshi Kobayashi,Jie Fan
The treatment of lean methane from sulfur-containing exhausts of natural gas vehicles is important, but challenging, because of the strong and irreversible catalyst poisoning by sulfur species. Herein, sulfur-tolerance enhanced NiO nanocatalysts (NiO-SPP) are prepared via surface polymeric phosphate (SPP) modification of NiO NPs, and a self-catalyzed sulfation (SCS) mechanism is proposed to reveal the insights on the boosted sulfur-tolerance capacity. In the SCS mechanism, the sulfation process is divided into two steps, namely, (1) the initial sulfation process and (2) the self-catalyzed sulfation process accelerated by previously formed sulfates. Stability and durability tests of NiO NPs and NiO-SPP reveal that the improved sulfur-tolerance capacity via SPP modification should be attributed to two reasons, namely, the external reason as the protection on the surface structure of NiO-SPP, and the internal reason as the deceleration of the initial sulfation rate. Specifically, characterizations and analyses of the catalysts before and after stability tests indicate that the SPP modification could efficiently cut down the interaction possibility of active sites with sulfur species. On the basis of the SCS mechanism, the initial sulfation rate of NiO NPs under long-term deactivation tests is calculated to be 12.8 times that of NiO-SPP, and the self-catalyzed sulfation rate constant for NiO NPs is twice that of NiO-SPP. The SPP modification of catalysts and the SCS mechanism provide a reference for the improvement of catalyst durability on the perspective of deactivation.
研究领域:
催化
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Future Foods
·
2022
·
IF 0
Hui Hao,Chun Cui,Yuqing Xing,Xuewei Jia,Bingjie Ma,Wenyi Kang,Tianxiao Li,Mingqi Gao,Chunping Xu
ABSTRACT An acidic extracellular polysaccharide (EPS) was gained from Stropharia rugosoannulata fermentation broth by the alcohol precipitate method. Sulfated derivative of EPS (S-EPS) was gained by chlorosulfonic acid-pyridine method and the conditions were as follows: pyridine as the reaction solvent, temperature 80 °C, and reaction time 90 min. The chemical structure and chain conformation of EPS and S-EPS were characterized by FT-IR, NMR and size exclusion chromatography (SEC) coupled with multi-angle laser light scattering (MALLS) detection. FT-IR spectra indicated the sulfated group was linked to the polymer. The EPS mainly consists of mannose, glucose and glucuronic acid, and the glycosidic bond type is mainly (1→4)-linked and (1→6)-linked. C6 and C2 were substituted by sulfate groups. The EPS was degraded into smaller molecular weight ( M w ) polysaccharides with the larger molecular size ( R g ) after sulfation and S-EPS has better hydrophilicity. Moreover, the sulfated derivative S-EPS was found to have a better scavenging ability on hydroxyl radicals than EPS. It proved that the chemical modification of S. rugosoannulata polysaccharides by sulfation effectively enhanced their antioxidant activity.
研究领域:
生物化学
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食品科学
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药理学
✓ 已验证
DOI
Foods
·
2022
·Vol. 11
·期 15
·2381
·
IF 5.561
Lilong Wang,Linlin Wang,Chunhong Yan,Chunqing Ai,Chengrong Wen,Xiaoming Guo,Shuang Song
The present study aimed to clarify the potential mechanism of fucoidans found inAscophyllum nodosumon anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found inA. nodosum, were separated and purified and their structures were elucidated by HPGPC, HPLC, GC-MS, FT-IR, NMR, and by the Congo red test. They both possessed a backbone constructed of →2)-α-L-Fucp4S-(1→, →3)-α-L-Fucp2S4S-(1→, →6)-β-D-Galp-(1→, and →3,6)-β-D-Galp4S-(1→ with branches of →2)-α-L-Fucp4S-(1→ and →3)-β-D-Galp-(1→. Moreover, ANP-6 and ANP-7 could prevent the inflammation of the LPS-stimulated macrophages by suppressing the NO production and by regulating the expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6, and IL-10. Their inhibitory effects on the TLR-2 and TLR-4 levels suggest that they inhibit the inflammation process via the blocking of the TLR/NF-κB signal transduction. In addition, ANP-6, with a molecular weight (63.2 kDa), exhibited stronger anti-inflammatory capabilities than ANP-7 (124.5 kDa), thereby indicating that the molecular weight has an influence on the anti-inflammatory effects of fucoidans.Keywords:sulfated polysaccharides;chemical structure;anti-inflammation;molecular weight
研究领域:
海洋生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Zhonghua Wang,Tongqiang Cai,Xipu He
Millettia dielsiana is a traditional herb medicine to treat rheumatic arthritis and gynecological diseases. In this study, a novel polysaccharide (MDP1) with a molecular weight of 139.54 kDa was isolated from Millettia dielsiana by DEAE-52 cellulose chromatography. Its sulfated derivative (S-MDP1) was prepared by the chlorosulfonic acid-pyridine method. Structural analysis indicated that MDP1 was a pectic polysaccharide and did not exhibit a triple helical conformation. In vitro , MDP1 and S-MDP1 had protective effect on H 2 O 2 -induced PC12 cells death. Immunological assay showed that MDP1 and S-MDP1 significantly stimulated NO production and phagocytic activity in RAW264.7 cells, and promoted lymphocyte proliferation . Anticoagulant assay indicated that S-MDP1 effectively prolonged activated partial thromboplastin time (APTT) and thrombin time (TT), but not prothrombin time (PT), and MDP1 prolonged APTT and TT weakly. These results implied that MDP1 and S-MDP1 had the potential to be used as antioxidant, immunomodulatory and anticoagulant agents.
研究领域:
药理学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xiaoya Tao,Yuanyuan Hu,Zhengqi Liu,Kaiyu Jiang,Jinjin Li,Xiaoming Guo,Beiwei Zhu
Protein-polysaccharide delivery systems function as a promising tool to deliver bioactive ingredients aiming to improve their solubility and bioavailability. In this study, gelatin (Gel) and κ-carrageenan (Car) were used to evaluate the characteristics of the Gel-Car emulsion and the emulsion-templated oleogels. Results showed that Car addition significantly reduced the particle size and increased the potential absolute value during emulsion storage, especially for Gel-0.5% Car and Gel-1.0% Car emulsions, indicating Car addition enhanced the emulsion's stability. The emulsions' rheological results indicated that all the emulsions exhibited weak gel-like behavior dominated by elasticity, suggesting Car addition improved the emulsions' shear resistance and viscoelasticity. The emulsions' instability indices were Gel (0.034), Gel-0.1% Car (0.026), Gel-0.5% Car (0.013), and Gel-1.0% Car (0.011), indicating Car addition enhanced the emulsions' stability. For oleogels, Car addition significantly reduced the oil loss rate, delayed the thermal degradation, and improved the viscoelasticity, shear resistance, thixotropic recovery properties, and high-temperature resistance, especially for Gel-1.0% Car oleogel. The stability analysis exhibited that Car addition improved the oleogels' stability, with Gel-0.5% Car oleogel showing the best stability. FFA release was Gel (25.22%), Gel-0.1% Car (26.86%), Gel-0.5% Car (31.38%), and Gel-1.0% Car (39.57%), suggesting Car addition promoted the rapid release of FFA during oleogels digestion. Results above indicated that Gel-Car oleogels exhibited good structural stability and high gel strength, which provide a theoretical basis and new ideas for the preparation of oleogels from protein-marine sulfated polysaccharides using the emulsion template method for food industrial application.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2022
·
IF 6.437
Hao Qi,Qirong Yang,Zhaoying Li,Chenyu Yang,Xinru Ma,Haoxi Ben
The mechanism of gas-phase products from pyrolysis of polysaccharides from Enteromorpha prolifera (PE) is researched. The sulfated polysaccharides are the most important polysaccharides in Enteromorpha prolifera . The molecular dynamics (MD) method is used to simulate the pyrolysis process of sulfated polysaccharides of Enteromorpha prolifera . Combined with the results of the pyrolysis simulations and density functional theory (DFT), rhamnose , glucuronic acid , glucose, and xylose are used as model compounds to estimate and calculate the reaction paths of hydrogen and carbon monoxide . In addition, fixed-bed pyrolysis experiments are performed with the above four monomers . The pyrolysis simulation results show that the pyrolysis process of sulfated polysaccharides is approximately divided into three stages: low-temperature, medium-temperature, and high-temperature stages. Gas-phase products are mainly produced in the high-temperature stage, and the main components of syngas are hydrogen, methane, and carbon monoxide. The simulation and experimental results show that pyrolysis of xylose is the most likely to generate hydrogen, followed by glucose, rhamnose, and glucuronic acid. The process of producing carbon monoxide from monomers goes through reactions such as dehydration, isomerization , C-C bond breakage, and decarbonylation . Moreover, the results of fixed-bed experiments indicate that when the four monomers are pyrolyzed individually, the highest proportions of hydrogen, carbon monoxide, and methane in the gas-phase products are obtained from the pyrolysis of xylose, glucuronic acid, and rhamnose. When the four monomers are mixed and then pyrolyzed, the proportion of hydrogen and carbon monoxide in the obtained gas-phase products decreases, and the proportion of carbon dioxide increases.
研究领域:
生物化学
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能源科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 18
·2865
·
IF 5.561
Jinghe Sun,Shuang Song,Chunqing Ai,Beiwei Zhu,Jingfeng Yang
Sulfate polysaccharides, such as heparin sulfate, have been found to have inhibitory activity against SARS-CoV-2. An abalone polysaccharide, AGSP, was deeply sulfate modified using the chlorosulfonic acid/pyridine method, yielding S-AGSP. AGSP and S-AGSP inhibitions of SARS-CoV-2 infection of Vero E6 cells were tested in vitro. The interference of AGSP or S-AGSP on the binding interaction between the SARS-CoV-2 spike protein and angiotensin-converting enzyme was tested using a biolayer interferometry assay. Results showed that S-AGSP, above a concentration of 1.87 µg/mL, significantly inhibited SARS-CoV-2 infection of Vero E6 cells. Compared with AGSP, S-AGSP obviously weakened the affinity between the SARS-CoV-2 spike protein and ACE2. The polysaccharide’s sulfate content played a vital role in influencing the binding affinity of spike protein to ACE2. Therefore, S-AGSP has potential as a COVID-19 competitive inhibitor as well as a candidate to be repurposed as a prophylactic COVID-19 therapeutic.Keywords:sulfated;polysaccharide;SARS-CoV-2;biolayer interferometry;ACE2
研究领域:
病毒学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
·
2017
·Vol. 53
·期 1
·85-93
·
IF 0.659
Fan N.,Du C. H.,Xu J. Q.,Xu Y. X.,Yu B. Y.,Zhang J.
The aim of the study was to expand the substrate scope of Gliocladium deliquescens NRRL 1086 and generate coumarin glycosides using 4-methylumbelliferone (4-MU) as a substrate. The obtained results indicated that 4-MU can be metabolized to its glucoside (M1) and sulfate conjugate (M2), and the structures of the metabolites were elucidated by spectroscopic or enzymatic methods. Time course experiments detected that nearly 90% of 4-MU could be metabolized within 24 h, and the maximum yield of M1 could reach as high as 32%. Further tests revealed that the glucose concentration in the medium had little effect on the yield of M1 but time of 4-MU-adding could markedly affect the glycosylation procedure, and the favorable time to accumulate M1 was in the 12 or 24 h-old stage II culture, while in the 36 h-old or even older stage II culture, the substrate was almost metabolized to M2. The attempts to alter the ratio between M1 and M2 were performed by addition of quercetin and S-tetrahydroberberrubine or reduction of the sulfate concentration in the culture medium. Herein we describe, to the best of our knowledge, the first example of simultaneously microbial glycosylation and sulfation of coumarins.
研究领域:
微生物学
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有机化学
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药理学
✓ 已验证
DOI
高影响因子
ChemSusChem
·
2010
·Vol. 4
·期 1
·79-84
·
IF 4.767
Zhi Wang,Qiang Lu,Xi-Feng Zhu,Ying Zhang
Graphical It′s not diamond, it′s zirconia: SO 4 2− /ZrO 2 is an efficient catalyst for the production of levoglucosenone by fast pyrolysis of cellulose admixing catalysts. The optimal temperature for preparation of levoglucosenone is in the range of 320–350 °C. In the presence of the SO 4 2− /ZrO 2 , the levoglucosenone content of pyrolysis liquid is greatly increased at 335 °C compared to pure cellulose. Sulfated zirconia was employed as catalyst for fast pyrolysis of cellulose to prepare levoglucosenone (LGO), a very important anhydrosugar for organic synthesis. The yield and the selectivity of LGO were studied in a fixed-bed reactor at different temperatures and cellulose/catalyst mass ratios. The experiments of catalyst recycling were also carried out. The results displayed that from 290 to 400 °C, the liquid and solid accounted for more than 95 wt % of products, and the higher temperature led to more liquid and less solid products. The introduction of SO 4 2− /ZrO 2 could promote cellulose conversion and LGO production. The temperature had a similar effect on the yield and selectivity of LGO at different cellulose/catalyst mass ratios. The maximum yield was obtained at 335 °C. Although the structure of the parent ZrO 2 was retained after recycles, which was confirmed by X-ray diffraction and N 2 adsorption–desorption measurements, the activity of SO 4 2− /ZrO 2 could only be partially recovered by simply calcination. The catalytic activity decrease could be mainly attributed to SO 4 2− leaching, and the activity could be restored by further impregnation of H 2 SO 4 .
研究领域:
化学工程
·
生物质能
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催化科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Xuehui Li,Yongming Lu,Wenna Zhang,Shixiong Yuan,Liyuan Zhou,Liming Wang,Qiuying Ding,Dandan Wang,Weiwei Yang,Zhengnan Cai,Yan Chen
Polysaccharide TLH-3 from the fruit body of Tricholoma lobayense Heim has shown outstanding antioxidant activity. In order to further explore it, TLH-3 was successfully modified to obtain a sulfated derivative (STLH-3). The chemical characteristics of STLH-3 and TLH-3 were determined by high performance liquid chromatography and infrared spectroscopy. Antioxidant activity and cytotoxicity of the samples were investigated in vitro . The antioxidant activities of STLH-3 were significantly improved. In the four indicators of antioxidant activity, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion scavenging activities of STLH-3 have exceeded that of Vitamin C (Vc). The cytotoxicity against tumor cells including human cervical carcinoma cell line (HeLa) and human breast cancer cell line (MCF-7) of STLH-3 was obviously stronger than that of TLH-3. STLH-3 had no toxic on human embryonic lung fibroblasts (HELF) cells. These results suggested that STLH-3 would be a promising bioactive macromolecule for potential applications in the field of health care and pharmaceutical.
研究领域:
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Hao Tang,Jinwen Huang,Qingxia Yuan,Kunling Lv,Haiqiong Ma,Tingting Li,Yonghong Liu,Shunli Mi,Longyan Zhao
Chlorella is one of the most widely cultivated species of microalgae and has been consumed as a “green healthy food”. In this study, a novel polysaccharide (CPP-1) was isolated from Chlorella pyrenoidosa , structurally analyzed, and sulfated as a promising anticoagulant . Structural analyses by chemical and instrumental methods such as monosaccharide composition , methylation-GC–MS and 1D/2D NMR spectroscopy analysis revealed that CPP-1 had a molecular weight of ~13.6 kDa, and mainly consisted of d -mannopyranose ( d -Man p ), 3- O -methylated d -Man p (3- O -Me- d -Man p ), and d -galactopyranose ( d -Gal p ). The molar ratio of d -Man p and d -Gal p was 1.0:2.3. CPP-1 consisted of a (1→6)-linked β- d -Gal p backbone substituted at C-3 by the d -Man p and 3- O -Me- d -Man p residues in a molar ratio of 1:1, which was a regular mannogalactan. The sulfated Chlorella mannogalactan (SCM) with sulfated group content of 40.2 % equivalent to that of unfractionated heparin was prepared and analyzed. NMR analysis confirmed its structure, indicating that most free hydroxyl groups in the side chains and partial hydroxyl groups in the backbone were sulfated. Anticoagulant activity assays indicated that SCM exhibited strong anticoagulant activity by inhibiting intrinsic tenase (FXase) with IC 50 of 13.65 ng/mL, which may be a safer anticoagulant as an alternative to heparin-like drugs.
研究领域:
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）