INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Peilin Chen,YuShan Huang,Hongliang Zeng,Mingjing Zheng,Juanjuan Guo
This study systematically investigated the modulatory effects of Porphyra haitanensis polysaccharides (PHP) and its two P. haitanensis polysaccharide components (PHP40 and PHP80) on gut microbiota in ovalbumin (OVA)-sensitized mice via in vitro fermentation. PHP40 and PHP80 exhibited sulfate contents of 5.94 ± 0.05 % and 11.72 ± 0.03 %, respectively, with galactose and glucose as dominant monosaccharides. The different polysaccharide components had different effects on the aforementioned changes in the species composition and structure of the gut microbiota in allergic mice, with distinct dominant microbial profiles across groups. PHP could promote Bacillus and Enterococcus proliferation while inhibiting Staphylococcus . PHP40 and PHP80 could promote Enterococcus and Enterobacter growth but suppressed Staphylococcus and Bacillus . Functional prediction indicated PHP significantly improved galactose metabolism and primary/secondary bile acid biosynthesis, potentially alleviating allergic responses.
研究领域:
微生物学
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免疫学
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营养学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Yan Ma,Na Gao,Zhichuang Zuo,Shanni Li,Wenqi Zheng,Xiang Shi,Qipei Liu,Ting Ma,Ronghua Yin,Xian Li,Jinhua Zhao
Fucan sulfates from echinoderm possess characteristic structures and various biological activities. Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis . Chemical composition analysis showed that they were all composed of l -fucose. Their sulfate content was determined by a conductimetric method. The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively. Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)- l -Fuc S -α(1→, where Fuc S were Fuc 2S4S (10%), Fuc 2S (44%), Fuc 0S (10%), Fuc 4S (36%), that of FSIII was →4)- l -Fuc 2S -(α1 → 4)- l -Fuc 2S -(α1 → 4)- l -Fuc 0S/3S -(α1→, where Fuc 0S and Fuc 3S were in equal molar, and that FSIV was →4)- l -Fuc 2S3S -(α1 → 4)- l -Fuc 2S3S -(α1 → 4)- l -Fuc 2S -(α1→4)- l -Fuc 2S -(α1 → 4)- l -Fuc 2S -(α1 → 4)- l -Fuc 2S -(α1 → . This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species. Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT. Among them, FSII, FSIII and FSIV showed the similar potency, while FSI owned the strongest. Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.
研究领域:
海洋生物学
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生物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Bilal Muhammad Khan,Hua-Mai Qiu,Xue-Fei Wang,Zhuo-Yan Liu,Jia-Yan Zhang,Yan-Jun Guo,Wei-Zhou Chen,Yang Liu,Kit-Leong Cheong
A complete absence of the physicochemical characterization of Gracilaria chouae polysaccharides (GCP) and its corresponding bioactivities urged the need for this study. It was found that GCP is a heteropolysaccharide which exists in linear random coil conformation. It contained a sulfate content of 7.9% in addition to 52.63% total sugar content and 9.62% galacturonic acid. Galactose and 3,6-anhydrogalactose were found in a molar ratio of 1.0:0.6. Its setting and melting points were determined as 41.3 and 71.7 °C, respectively, which makes it a suitable candidate for industrial processing where further heating is required and/or where the end-product needs to have extended shelf life in hot climate. GCP demonstrated 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis and hydroxyl radical scavenging activity (36.86, 27.42 and 19.07% at 3 mg·ml −1 ). Moreover, the results also suggested its potential use as a prebiotic due to its perceived high fermentability.
研究领域:
海洋生物学
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药学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）