INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Lijun Shao,Yue Sun,Jin Liang,Meiqing Li,Xueling Li
Different decolorization methods have different effects on the properties of polysaccharides . In this study, polysaccharides extracted from Thesium chinense Turcz ( T. chinense ) were decolorized using either hydrogen peroxide (H 2 O 2 ), which has been previously reported, or activated carbon. To begin, the conditions for decolorization by activated carbon were optimized through single factor experiments and orthogonal modeling and were predicted to be best at 0.5% (w/w) activated carbon, pH 5.0 and 40 °C in a water bath for 60 min. Following treatment by the two methods, the structures of the decolorized polysaccharides were compared. The samples treated by activated carbon (CTP) had higher molecular weight (Mw: 3.064 × 10 5 g/mol) and more sulfate groups (14.33%, w/w), whereas the samples treated by H 2 O 2 (HTP) had lower Mw (8.349 × 10 4 g/mol) and more uronic acid (35.02%, w/w). The antioxidant capacities of the two samples were analyzed by free radical scavenging (DPPH, hydroxyl and ABTS) and cell antioxidant activity (CAA). The HTP showed a better scavenging effect on free radicals and a higher CAA. Together, the results showed that various decolorization methods differentially influence the characteristics of the polysaccharides extracted from T. chinense .
研究领域:
植物化学
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食品科学
·
生物化学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
·
2022
·Vol. 34
·期 6
·2755-2767
·
IF 3.404
Huo Shuhao,Wang Huiying,Chen Jing,Hu Xinjuan,Zan Xinyi,Zhang Cunsheng,Qian Jingya,Zhu Feifei,Ma Haile,Elshobary Mostafa
The total polysaccharide content of the sugar-rich filamentous microalga Tribonema minus (Xanthophyceae, Tribonematales) under 2% CO 2 was up to 50%. The optimal conditions for the extraction of polysaccharide by hot water extraction-ethanol deposition were as follows: A maximum polysaccharide yield of 27.25% was obtained under the optimized conditions of extraction temperature 80°C, extraction time of 2 h, pH 9, and liquid to raw material 0.5:1 mL mg −1 . The crude polysaccharides were mainly acidic polysaccharides containing sulfate groups and uronic acid, and the contents of which were 14.57% and 42.21 mg g −1 , respectively. The crude polysaccharide was mainly composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 0.31:0.26:0.40:0.42:1.00:0.47. In addition, the polysaccharide had a potential antioxidant activity in vitro. The scavenging effect and reducing power of polysaccharides increased with the increase of polysaccharide concentration. The scavenging effect was 56.11%, 75.6%, and 61.89% for 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), superoxide, and hydroxyl radicals at a concentration of 2 mg L −1 , respectively. Hence, T. minus polysaccharides could be a source of natural antioxidants that might be used in foods or pharmaceuticals.
研究领域:
藻类学
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生物化学
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食品科学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Lihua Geng,Quanbin Zhang,Qiong Li,Qian Zhang,Cong Wang,Ni Song,Wenyu Xin
Silvetia siliquosa , the only species of the family Fucaceae in China, is used as a medicine food homology. Fucoidan from S. siliquosa was extracted by hot water twice thoroughly (13 % of total yield), and a purified fucoidan SSF with a molecular weight of 93 kD was obtained. Chemical composition analysis demonstrated that SSF was primarily composed of sulfate (21.68 wt%) and fucose (84 % of all neutral monosaccharides). IR, methylation analysis, NMR and ESI-MS results indicated SSF had the backbone of mainly (1 → 3)-α-L-fucopyranose and minor (1 → 4)-α-L-fucopyranose, with little 1,3 and 1,4 branched β-D-Xyl p and β-D-Gal p . The in vitro immunomodulatory test on RAW 264.7 cells showed that SSF could up-regulate the expression of immune related factors and proteins in a concentration-dependent manner, but the immunomodulatory effect disappeared from desulfated SSF . This research indicated that highly sulfated fucan possessed immunomodulatory effect and the importance of sulfate groups in the activity of SSF .
研究领域:
药理学
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免疫学
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海洋生物学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ying You,Chen Song,Yinghuan Fu,Yujiao Sun,Chengrong Wen,Beiwei Zhu,Shuang Song
Caulerpa lentillifera polysaccharide (CLP) has been characterized as a sulfated polysaccharide which can effectively inhibit lipid digestion. However, little information was known regarding its inhibitory mechanisms. In the present study, desulfation and degradation were conducted to prepare the derivatives of CLP, and a series of chemical and spectroscopic methods were used to elucidate the structure-activity relationship of CLP on the inhibitory effect of lipid digestion. Results revealed that CLP possessed excellent binding capacities for sodium cholate, sodium glycocholate, and sodium taurocholate. In addition, CLP can effectively inhibit lipase activity by quenching the fluorescence intensity, changing the secondary structure , and decreasing the UV–Vis absorbance. Of note, sulfate groups in CLP took a vital role in inhibiting lipase activity, while the molecular weight of CLP showed a positive correlation with the binding activities of bile acids . Furthermore, adding CLP into the whey protein isolate (WPI) emulsion system also impeded lipid digestion, indicating that CLP can be a potential reduced-fat nutraceutical used in food emulsion systems.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Shuang Sun,Lijie Zhao,Jirui Yang,Xiaoqi Wang,Xiaoya Qin,Xinhua Qi,Feng Shen
A green, efficient, and facile ball-milling-assisted strategy was proposed to design an SO3H-containing solid acid for biomass valorization, where −SH groups were grafted on a natural clay attapulgite by wet ball milling with 3-mercaptopropyltrimethoxysilane (MTPS), followed by the oxidation of −SH to −SO3H with H2O2 at room temperature. The mechanism of the −SO3H functionalization by the ball-milling process was investigated, and the investigation indicated that the MTPS was grafted on the attapulgite through hydrolysis and condensation, which was induced by the mechanical forces of ball milling. Characterization, including SEM, EDX (energy-dispersive X-ray spectrometry), NH3-TPD, TGA, FT-IR, elemental analysis, and XPS, confirmed the success of −SO3H modification on attapulgite with a total acidity of 1.81 mmol/g. Harsh conditions involving a corrosive sulfonating agent, organic solvent, or high temperature in traditional methods are avoided in the developed approach. The prepared catalyst displayed a high activity toward the transformation of biomass-derived carbohydrates (glucose, fructose, cellobiose, sucrose, starch, and cellulose) into the more valuable chemical 5-hydroxymethylfurfural due to the synergy effect of MgO and −SO3H sites on the catalyst. Moreover, the catalyst showed excellent stability and recyclability for five recycles without loss of activity. This study provides a novel green method for the preparation of an SO3H-containing heterogeneous catalyst, which should have great application potential in other processes of biomass valorization.
研究领域:
化学工程
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催化材料
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绿色化学
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产品关联
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 5
·1015
·
IF 7
Xue-Wu Chen,Yu-Yun Zheng,Jian-Ming Ouyang
The clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four different sulfated levels ofUndaria pinnatifidapolysaccharides (UPP0, UPP1, UPP2, and UPP3 with sulfate group [–OSO3−] contents of 1.59%, 6.03%, 20.83%, and 36.39%, respectively) on the repair of oxidatively damaged HK-2 cells. The results showed that after repair by UPPs, cell viability was enhanced, healing ability was improved, the intracellular superoxide dismutase level and mitochondrial membrane potential were increased, malondialdehyde, reactive oxygen species, and intracellular Ca2+levels were reduced, cellular autophagy was reduced; lysosomal integrity was improved, and cytoskeleton and cell morphology were restored. The ability of repaired cells to endocytose nano-calcium oxalate dihydrate crystals (nano−COD) was enhanced. The activity of UPPs was closely related to their –OSO3−content. A too high or too low –OSO3−content was not conducive to polysaccharide activity, and only UPP2 exhibited the best cell repair ability and strongest ability to promote the cell endocytosis of crystals. UPP2 may be used as a potential agent to inhibit CaOx crystal deposition caused by high oxalate concentration.Keywords:Undaria pinnatifidapolysaccharide;acidic groups;cell repair;crystal endocytosis;primary hyperoxaluria
研究领域:
肾病学
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Ziyu Li,Kit-Leong Cheong,Bingbing Song,Huan Yin,Qian Li,Jing Chen,Zhuo Wang,Baojun Xu,Saiyi Zhong
To accurately, efficiently, and environmentally prepare carrageenan oligosaccharides, we have developed a method that uses H 2 O 2 and TiO 2 as catalysts for the photodegradation of κ-carrageenan (KC). The photodegradation of KC was monitored using various amounts of TiO 2 and H 2 O 2 and different concentrations of KC via HPLC and it could decrease the average molecular weight of KC into 1.6 kDa within 2 h. Further research under optimal conditions. As a control, the effects of UV, UV/H 2 O 2 , UV/TiO 2 , and H 2 O 2 /TiO 2 treatments were studied. In contrast, UV/H 2 O 2 /TiO 2 treatments showed a coordinated effect. The effect of degradation on the structure of KC was investigated by FT-IR, XRD, and there was no obvious remotion of sulfate groups. Furthermore, oral administration of KCO prolonged the healthy lifespan of nematodes induced by ultraviolet stress and significantly regulated oxidative stress. This study suggests that the precise preparation and application of KCO may be beneficial.
研究领域:
食品科学
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Chen Song,Zhejin Chen,Linlin Wang,Nan Wang,Jie Tian,Chunqing Ai,Shuang Song
Low-molecular-weight carrageenan has attracted great attention in the food and medical industries due to its excellent solubility and diverse biological activities. However, the development of environmentally friendly methods for low-molecular-weight carrageenan production is still in great need. In the present study, a photocatalysis-freeze-thaw circle degradation method of κ-carrageenan was developed based on its two-phase properties after freezing and thawing. Interestingly, the addition of KCl with the ability to enhance the gel strength could lead to the molecular weight decrease of the prepared low-molecular-weight carrageenan whose relative molecular weight could reduce to ≤2.8 kDa. Moreover, FT-IR, ESI-MS n , NMR, and TEM analysis results showed that the photocatalysis-freeze-thaw circle method could separate the degraded low-molecular-weight carrageenan in time to avoid overreaction during the reaction process, which could fully protect the sulfate groups of low-molecular-weight carrageenan from destruction and produce less by-products. In addition, the digestion and distribution of low-molecular-weight carrageenan in vivo were tracked after fluorescent labeling, and the results demonstrated that low-molecular-weight carrageenan could enter the liver and kidney after oral administration. Thus, the present study demonstrated a novel method to prepare low-molecular-weight carrageenan and revealed in vivo distribution after oral administration.
研究领域:
食品科学
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生物医学
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DOI
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2016
·Vol. 34
·期 1
·110-117
·
IF 1.408
Zhu Jundong,Gan Linhuo,Li Baoxia,Yang Xin
Three kinds of solid acid catalysts were prepared from alkali lignin in the waste liquor of pulping using carbonation- sulfonation method with different pretreatment. The lignin-derived solid acids (LDSAs) were characterized by FESEM, XRD, FTIR, TGA, BET and acid-base titration, respectively. A comparison study on the catalytic performance of LDSA prepared by different pretreatment method before carbonation in the hydrolysis of microcrystalline cellulose (MCC) was carried out. Results showed that the LDSA prepared by chemical activation with phosphoric acid (LPC-SO 3 H) exhibited superior catalytic activity due to its higher densities of -COOH group (1.68 mmol/g) as binding site and -SO 3 H group (0.88 mmol/g) as catalytic site as well as its larger specific surface area (488.4 m 2 /g) than those of the other two LDSAs. A total reducing sugar yield of 50.8% in MCC hydrolysis was obtained under the reaction conditions of temperature of 180 °C, time of 3 h, MCC concentration of 6 mg/mL and mass ratio of catalyst to MCC of 3.3 (w/w). Additionally, the value activation energy for hydrolysis of MCC to reducing sugars using LPC-SO 3 H was 83.31 kJ/mol, which was smaller than that using sulfuric acid.
研究领域:
生物化学
·
可再生资源与环境工程
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Mingjing Zheng,Liyuan Wang,Tao Hong,Zhipeng Li,Yanbing Zhu,Zedong Jiang,Hui Ni,Yuanfan Yang,Yujia Ou
This study investigated the effects of ultra-high pressure (UHP)-assisted alkaline extraction on the physicochemical and structural properties of carrageenan. UHP treatment between 100 and 300 MPa significantly enhanced performance, with yield increasing by 12.7% at 100 MPa and gel strength reaching 911.57 g/cm 2 at 300 MPa, accompanied with a 16.15% improvement when compared with the control. Compositional analysis showed a 13.26% reduction in sulfate groups and a 19.63% increase in 3,6-anhydrogalactose at 300 MPa, together with higher uronic acid (increased by 30.11%) and total sugar (increased by 13.90%) contents. These changes resulted in functional improvements, e.g., the elevated water-holding capacity. LF-NMR analysis indicated the reduced water mobility and a higher proportion of bound water for carrageenan treated at 300 MPa. Antioxidant assays further confirmed that carrageenan treated at 300 MPa exhibited stronger radical scavenging activity, with DPPH and •OH clearance rates increased by 20.99% and 8.87% ( p < 0.05), respectively, whereas treatment at 500 MPa attenuated these effects. Overall, the results demonstrated that moderate UHP treatment, particularly at 300 MPa, effectively improved the structural and functional properties of carrageenan, supporting its potential application as a functional hydrocolloid.
研究领域:
食品科学
·
高分子化学
·
天然产物化学
·
功能食品
·
生物材料
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产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Jiaqi Song,Liang Shao,Hongwen Yu,Caiting Meng,Guanying Li
Heparan sulfate proteoglycans (HSPGs) play a crucial role in regulating cancer growth and migration by mediating interactions with growth factors. In this study, we developed a self-assembling peptide (S1) containing a sulfate group to simulate the contiguous sulfated regions (S-domains) in heparan sulfate for growth factor binding, aiming to sequester growth factors like VEGF. Spectral and structural studies as well as simulation studies suggested that S1 self-assembled into nanostructures similar to the heparan sulfate chains and effectively bound to VEGF. On cancer cell surfaces, S1 self-assemblies sequestered VEGF, leading to a reduction in VEGF levels in the medium, consequently inhibiting cancer cell growth, invasion, and angiogenesis. This study highlights the potential of self-assembling peptides to emulate extracellular matrix functions, offering insights for future cancer therapeutic strategies.
研究领域:
癌症生物学
·
分子生物学
·
纳米技术
·
药学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Zhixiang Cui,Hezhi Wang,Lu Qin,Ye Yuan,Jingwen Xue,Yalin An,Le Sun,Renfang Zhu,Qingyu Li,Yi Wang,Shuman Cui,Xuanguang Zhan,Qiyao Zhai,Haiyan Sun,Xin Zhang,Jian Guan,Chang Liu,Shirui Mao
The immunomodulatory effects and excellent tolerability of polysaccharides make them optimal candidates for pulmonary vaccine adjuvants. Yet, the structure–immunostimulatory activity relationship of polysaccharides remains unrevealed. Here, we developed nanovaccines decorated with four polysaccharides of distinct structures─hyaluronic acid (HA), pectin (PC), chondroitin sulfate (SC), and heparan sulfate (SH)─all sharing similar particle sizes and zeta potential. Polysaccharides containing sulfate groups (SC, SH) exhibited superior efficacy in overcoming natural inhalation barriers and recruiting dendritic cells (DC). DC stimulation assays revealed that HA and SH significantly upregulated the expression of costimulation signals, with IL-6 secretion rising over 8.7-fold compared to pure OVA. Fluorescence resonance energy transfer demonstrated their detachment within the lysosomal microenvironment, thereby enhancing antigen cross-presentation. However, in vivo findings only showed that SH upregulated CCR7 chemokine and swiftly migrated to lymph nodes. Molecular docking and Western blot analyses further elucidated the involvement of the TLR─MyD88─TRAF6─NF-κB/MAPK/IRF-7 signaling pathways. Notably, SH-modified nanovaccines induced a more robust cellular and humoral immune response with the potential for immune memory. This study confirms that sulfate groups in polysaccharides enhance immune activation and that combining sulfate with acetyl groups offers a promising adjuvant configuration for augmenting mucosal, cellular, and humoral immunity.
研究领域:
免疫学
·
药剂学
·
分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）