Symmetry-Basel
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2023
·Vol. 15
·期 3
·629
·
IF 2.7
Fuchong Li,Wei Liu,Li Tian,Wei Cao,Xu Li,Junhong Guo,Jinfeng Cui,Baoping Yang
Because of easy functionalization, low cost, and large-scale fabrication, pure organic fluorescent polymers are widely applied in light-emitting display, bio-fluorescence-enhanced imaging, explosive detection, and other fields. Among these applications, due to their unique optical rotation characteristics, chiral fluorescent polymer materials are part of fluorescent polymers which could be used in chiral molecular detection and separation, biological target detection, etc. In this work, we designed and synthesized the first chiral organic fluorescent polysulfate materials through sulfur fluoride exchange polymerization (new click chemistry) by asymmetric binaphthol molecular. The chiral fluorescent polysulfate were synthesized by R/S [1,1′-binaphthalene]-2,2′-diol(Binol.), propane-2,2-diylbis(4,1-phenylene) bis(sulfurofluoridate) (FO2S–BA–SO2F) and 4,4′-(propane-2,2-diyl)diphenol(BA.) through step-by-step polymerization reaction under alkali present. It was found that the local crystallization of pure bisphenol A polysulfate was broken by the asymmetric axial chiral BINOL molecule inserted in it and let the polymer into the amorphous state. Fluorescent chiral molecules are uniformly dispersed in the polymer; the 120 µm film prepared by the film scraper was transparent and had good luminescence characteristics under ultraviolet light. After fluorescence detection, the excitation wavelength is 450 nm, and the emission wavelength is 480 and 517 nm.Keywords:polysulfate material;fluorescence;polymer;chiral binaphthol
研究领域:
有机化学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiong Li,Chuyi Li,Wanzi Yao,Jian Mao
Carrageenan oligosaccharides have attracted significant attention due to their excellent biological activities, and they can be produced by carrageenases. In this study, a novel marine bacterium, Shewanella sp. LE8, was used to enzymatically degrade carrageenan, and the κ-, ι-, and λ-carrageenases produced by the bacterium were characterized. The crude enzyme derived from media exhibited κ-carrageenase activity, indicating its natural secretion during growth and proliferation. According to the results of molecular weight distribution from HPGPC, λ-carrageenan could be degraded by Shewanella sp. LE8 cells in both 20 mM Tris-HCl buffer and PBS, while ι-carrageenan can only be hydrolyzed in PBS environment, indicating the activation of ι-carrageenase in Shewanella sp. LE8 was dependent on the presence of Na + ions. The components of carrageenan hydrolysates were determined by ESIMS, and results revealed that κ-carrageenan hydrolysates were primarily consisted of G4S-DA, [G4S-DA] 2 and [G4S-DA] 3 , while ι-carrageenan hydrolysates were predominantly composed of [G4S-DA2S] 2 . Moreover, the fragment ions of λ-carrageenan revealed that the hydrolytic process not only encompasses enzymatic cleavage of glycosidic bonds but also involves the participation of sulfatase. The precise structure was further elucidated through NMR analysis, confirming that the end-products of ι-carrageenase were neoι-carratetraose. The results highlight the potential of Shewanella sp. LE8 for industrial applications in the production of ι-carrageenan oligosaccharides.
研究领域:
海洋生物学
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酶学
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生物化学
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DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
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2014
·Vol. 37
·期 1
·183-188
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IF 1.736
He Yu-Cai,Tao Zhi-Cheng,Zhang Dan-Ping,Yang Zhen-Xing,Gao Shan,Ma Cui-Luan
Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. ( R )-1,2-Propanediol (>99 % e.e. ) was obtained at 44 % yield with growing cells. Glycol sulfide, ethylene sulfate, and 1,3-PDS were converted into the corresponding diols at 94.6, 96.3, and 98.3 %, respectively. Optimal reaction conditions with lyophilized resting cells were 30 °C, pH 7.5, and cell dosage 17.9 mg cell dry wt/ml. 1,3-Propanediol was obtained from 50 mM 1,3-PDS at 97.2 % yield by lyophilized cells after 16 h. Lyophilized cells were entrapped in calcium alginate with a half-life of 263 h at 30 °C, and the total operational time of the immobilized biocatalysts could reach over 192 h with a high conversion rate.
研究领域:
微生物学
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生物催化
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Xuemin Shen,Zhenfeng Liu,Junhui Li,Dongmei Wu,Meng Zhu,Lufeng Yan,Guizhu Mao,Xingqian Ye,Robert J. Linhardt,Shiguo Chen
Low molecular weight heparins (LMWHs) are currently used as an anticoagulant agent since unfractionated heparin (UFH) can cause serious adverse drug reactions. LMWHs are commercially prepared using different methods such as nitrous acid cleavage and β-elimination under strong reaction conditions or with harsh chemicals, which may cause the saccharide units within the polysaccharide backbone to be decomposed and noticeably modified. This study demonstrates an effective method for depolymerizing heparin via the production of large amounts of free radicals from H 2 O 2 /ascorbic acid and ultrasonic power; this results in highly pure products because ascorbic acid can decompose during the reaction, which is different from the previously reported H 2 O 2 /Cu 2+ method. The reaction conditions—including concentration of ascorbic acid, reaction temperature and intensity of ultrasonic power—were investigated and optimized. We found that the degradation behavior of heparin in this combined physicochemical process conformed to first-order reaction kinetics . The chemical composition and structures of different LMWHs were analyzed. The results showed the primary structure and sulfate esters were well preserved after the depolymerization , the major repeat units are (1–4)-linked glucosamine and iduronic acid . The further in vitro assays indicated that the LMWHs produced by H 2 O 2 /ascorbic acid with ultrasonic power have an anti-metastatic effect in A549 cells, which suggested the LMWHs rapidly prepared in this physicochemical way have a potential for anti-tumor metastatic function.
研究领域:
药理学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Xiaoyu Wang,Chunzhe Xu,Hantao Tian,Yu Pang,Jie Lv,Meng Li
Ferroptosis, a non-apoptotic programmed cell death modality, has been recognized as an emerging therapeutic target for cancer treatment, particularly with the rapid advancements in bionanotechnology. However, the insufficient intracellular Fe 2+ ions and low reactive oxygen species (ROS) production severely restrict the efficacy of ferroptosis at tumor sites. Herein, a pH-responsive multifunctional nanoplatform (p-COF@GOx-FeS@HA/ART) was constructed to achieve efficient tumor ferroptosis through self-supplied Fe 2+ ions and amplified ROS. In this system, the large specific surface area and mesoporous structure enabled the porphyrin-based covalent organic frameworks (p-COFs) to act as scaffolds and drug carriers for enhancing the catalytic activity of glucose oxidase-stabilized ferrous sulfide nanodots (GOx@FeS) and encapsulation of artesunate (ART). By oxidizing glucose (Glu) in tumor cells, GOx not only consumed Glu for starvation therapy but also promoted intracellular acidity and supplied hydrogen peroxide (H 2 O 2 ) in the tumor microenvironment (TME), which facilitated the FeS-mediated chemodynamic therapy (CDT) as well as the release of hydrogen sulfide (H 2 S) for accelerating the ROS generation. Moreover, the lowered acidic TME could simultaneously trigger the release of ART and Fe 2+ ions, thus exacerbating ART-mediated ferroptosis. Due to its photothermal and photodynamic behavior, the nanoplatform under laser irradiation could generate ROS storms in tumor cells for high-performance ferroptosis therapy, which was demonstrated both in cancer cells and tumor-bearing mice. This work provides a promising strategy for the simple construction of a multifunctional nanoplatform with TME-responsive and self-triggered ferroptosis, showing great potential in cascade amplification of ferroptosis therapy.
研究领域:
肿瘤学
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生物材料学
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药理学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
·
IF 2.8
Jun Zhang,Ze-hua Liu,Ke-meng Zhao,Zhi Dang,Yun Liu,Yu Liu
This work for the first time reported the complete transformation of 17β-estradiol (E2) to estrone (E1) by unknown wild-type enzyme present in the widely used commercial arylsulfatase derived from Helix pomatia . It was found that acetate could effectively inhibit the unknown enzyme with a half inhibitory concentration (IC 50 ) of 140.9 μM, while phosphate and citrate showed no inhibition. Since the buffer solutions with phosphate and citrate have been used in the enzymatic hydrolysis of natural estrogen conjugates for decades, the transformation of E2 to E1 likely occurred during such procedure, inevitably leading to overestimated E1, but underestimated E2. It was further suggested that acetate should be used to prevent this undesirable transformation during the enzymatic hydrolysis of natural estrogen conjugates.
研究领域:
生物化学
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环境科学
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医学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN TOXICOLOGY
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2019
·
IF 3.274
Ruihong Li,Wei Li,Yutong You,Xiucai Guo,Ying Peng,Jiang Zheng
Aloe-emodin (AE) is a major anthraquinone ingredient of numerous traditional Chinese medicines with a variety of beneficial biological activities in vitro. Previous studies suggested that AE possessed cytotoxicity and genotoxicity. Nevertheless, the mechanisms of the toxic action of AE have not yet been fully clarified. The present study aimed at characterization of metabolic pathways of AE to better understand the mechanisms of AE-induced cytotoxicity. An AE-derived glutathione conjugate (AE-GSH) was observed in rat liver cytosol incubations containing AE and GSH, along with 3′-phosphoadenosine-5′-phosphosulfate (PAPS). Similar incubation fortified with N-acetylcysteine (NAC) in place of GSH offered an AE-NAC conjugate corresponding to the GSH conjugate. The formation of the two conjugates was found to require PAPS. The two conjugates were respectively detected in bile and urine of rats given AE. Sulfotransferase (SULT) inhibitor pentachlorophenol (PCP) suppressed the production of the observed AE-GSH/NAC conjugates in vivo, which suggested that SULTs participated in the process of the metabolic activation of AE. The presence of PCP attenuated cell susceptibility to AE-induced cytotoxicity. The present study illustrated potential association of sulfation-mediated bioactivation of AE with its cytotoxicity.
研究领域:
药理学
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毒理学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2013
·
IF 2.596
Quancai Li,Chunxia Li,Cheng Yang,Chenchang Liu,Guangli Yu,Huashi Guan
Three derivatives of polymannuronic acid (PM) were prepared by chemically polyanionic modification. PM was substituted with phosphate ( OPO 3 2− ), H-phosphonate ( OPO 2 H − ) and sulfate ( OSO 3 − ) groups with the degree of substitution (DS) of 0.41, 1.42 and 1.04, respectively. The structures of all PM derivatives were characterized by FT-IR, 31 P NMR and 13 C NMR spectroscopy . The weight average molecular weight (Mw) was determined by high performance gel permeation chromatography (HPGPC). The antioxidant activities of PM and its derivatives were evaluated in vitro. The results indicated that both phosphate and H-phosphonate groups improved the hydroxyl radical scavenging activity, while sulfate group enhanced the superoxide radical scavenging activity. However, all of the derivatives scavenged DPPH less effectively than PM. The mechanism for how the different anionic substituent groups influenced the antioxidant activities was discussed.
研究领域:
生物化学
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食品科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 9
·4488
·
IF 6.208
Li Gao,Chen Xu,Xuelin Tao,Zhichuang Zuo,Zimo Ning,Linghui Wang,Na Gao,Jinhua Zhao
Fucan sulfate I (FSI) from the sea cucumberHolothuria fuscopunctatawas purified and its structure was clarified based on a bottom-up strategy. The unambiguous structures of a series of oligosaccharides including disaccharides, trisaccharides, and tetrasaccharides, which were released from mild acid hydrolysis of FSI, were identified by one-dimensional (1D)/two-dimensional (2D) nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. All the glycosidic bonds in these oligosaccharides were presented as α1,3 linkages confirmed by correlated signals from their1H-1H ROESY and1H-13C HMBC spectra. The structural sequence of these oligosaccharides formed by Fuc2S4S, Fuc2S, and non-sulfated ones (Fuc0S), along with the general structural information of FSI, indicated that the structure of FSI could be elucidated as: [-L-Fuc2S4S-α1,3-L-Fuc(2S)-α1,3-L-Fuc2S-α1,3-L-Fuc0S-α1,3-1-]n. Moreover, the L-Fuc0S-α1,3-L-Fuc2S4Slinkage in FSI was susceptible to be cleaved by mild acid hydrolysis. The antioxidant activity assays in vitro showed that FSI and the depolymerized product (dFSI′) had potent activities for superoxide radical scavenging activity with IC50of 65.71 and 83.72 μg/mL, respectively, while there was no scavenging effect on DPPH, hydroxyl and ABTS radicals.Keywords:fucan sulfate;mild acid hydrolysis;oligosaccharides;structure;antioxidant activity
研究领域:
海洋生物学
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分析化学
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生物化学
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DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2021
·
IF 7.328
Dedai Lu,Yuanyuan Jin,Xiangya Wang,Liyuan Xie,Qianqian Liu,Yamin Chen,Hui Wang,Ziqiang Lei
Due to the uncontrollable anticoagulant activity and limited source, Heparin, which is commonly used in clinical anticoagulation therapies, faces the risk of spontaneous bleeding and thrombocytopenia. Herein, a series of anionic poly(amino acid) s poly ( l -Serine- ran -L-Glutamic acid- ran -L-Cysteine-SO 3 ) (PSEC-SO 3 ) were prepared by the controlled Ring Opening Polymerization (ROP) of N-Carboxyanhydrides (NCAs). The anticoagulant activities of PSEC-SO 3 can be regulated by simply adjusting the feeding ratio of monomers. In vitro tests show that these polypeptides can effectively prolong the Activated Partical Thromboplastin Time (APTT) and inhibit Factor IIa and Factor Xa, but has no significant effect on Prothrombin Time (PT) and Thrombin Time (TT), which indicates that PSEC-SO 3 mainly act on the intrinsic pathway. In summary, the activity-tunable heparin-like polypeptides are expected to have good application prospects in the anticoagulant field.
研究领域:
生物化学
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材料科学
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医学
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DOI
PubMed
高影响因子
DESALINATION
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2014
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IF 3.96
Ling-Ling Shao,Quan-Fu An,Yan-Li Ji,Qiang Zhao,Xue-San Wang,Bao-Ku Zhu,Cong-Jie Gao
Sulfated carboxymethyl cellulose (SCMC) with tailored amount of sulfate groups was synthesized, and their composite nanofiltration membranes (SNFMs) were prepared by solution casting onto polysulfone (PSF) supporting membrane and crosslinking by glutaradehyde (GA). Chemical structure and composition of SCMCs and SNFMs were characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. SNFM surfaces were examined by field emission scanning electron microscopy , water contact angle and streaming potential measurement. The effects of the chemical composition on the hydrophilicity , the surface charge and the nanofiltration performance of SNFMs were determined. It is found that the incorporation of sulfate groups improves the water permeability of SNFMs while retaining their salt rejection. For the feed mixture of aqueous Na 2 SO 4 (1.0 g L − 1 , pH = 6.5), the optimum water flux is 39.6 L m − 2 h − 1 , which is 2.2 times of the pristine SNFM-0. For the xylenol orange (XO) dye solution (0.1 g L − 1 , pH = 4.0), the flux of SNFM-3 (30.0 L m − 2 h − 1 ) is also higher than that of SNFM-0 (11.1 L m − 2 h − 1 ). In addition, SNFM-3 exhibits good separation performance and chemical stability in treating saline anionic dye aqueous solution (MYB/NaCl) in acidic conditions.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2021
·
IF 9.127
Bo Pang,Qi Zhang,Xiaoming Yan,Xiaozhou Wang,Wanting Chen,Ruohan Du,Xuemei Wu,Minggang Guo,Gaohong He,Fujun Cui
Proton conductive group is essential to provide ion transport site and tune micro-phase separation in proton exchange membranes for vanadium redox flow battery (VRFB), while nearly all the fabrication methods are based on the benchmark sulfonic acid group. Herein, the sulfate ester group (-OSO 3 H) with superior acidity is proposed as a novel proton conductive group to achieve high proton transport at lower IECs, therefore disentangles the trade-off between vanadium crossover and conductive ion diffusion. The electron withdrawing ester oxygen atom endows sulfate ester group with 27 kJ mol −1 lower proton binding energy than the sulfonic acid group, leading to higher proton dissociation ability and better micro-phase separation. The 1,3-propanediol cyclosulfate grafted polybenzimidazole with IEC of 1.85 (OPBI-OSO 3 H-1.85) reaches comparable area resistance (0.19 Ω cm −2 ) to the sulfonic acid grafted polybenzimidazole with much higher IEC of 2.95 (OPBI-SO 3 H-2.95), but 19.1% decrease in vanadium permeability. The cell assembled with OPBI-OSO 3 H-1.85 achieves high energy efficiency of about 86.7% and low decay of discharge capacity (0.41% per cycle), superior to that of OPBI-SO 3 H-2.95 (0.51% per cycle) and Nafion 212 (0.71% per cycle). The sulfate ester group is proved to be chemically stable in H 2 SO 4 /vanadium environment.
研究领域:
材料科学
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电化学
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能源存储
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DOI
高影响因子
危险品化学品经营许可证（带存储）