JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2026
·
IF 4
Ao He,Juan Zhou,Jing Zhu,Guojing Hong,Minglan Fan,Yuyan Bao,Rong Li,Manxiao Cheng,Xiang Ran
Tumor hypoxia severely limits the therapeutic efficacy of photodynamic therapy (PDT), which relies on molecular oxygen to generate cytotoxic reactive oxygen species (ROS). Herein, an electrostatically assembled gold nanocluster–porphyrinic metal-organic framework nanozyme (AuNCs-pMOF) was developed as an oxygen-supplying platform for enhanced PDT of hypoxic tumors. By integrating catalase-mimetic gold nanoclusters (AuNCs) with a porphyrin-based metal-organic framework (pMOF), the resulting AuNCs-pMOF nanocomposite catalyzes the decomposition of endogenous H 2 O 2 to provide local O 2 , thereby supporting 1 O 2 /ROS generation under oxygen-limited conditions. In vitro studies demonstrated that AuNCs-pMOF maintained efficient ROS production and phototoxicity under hypoxia, whereas pristine pMOF showed reduced PDT efficacy. In vivo antitumor evaluation further confirmed that AuNCs-pMOF combined with light irradiation markedly inhibited tumor growth and induced pronounced tumor cell death, while showing favorable short-term tolerability under the tested experimental conditions. Overall, this work presents an electrostatically assembled oxygen-supplying nanozyme platform to mitigate oxygen shortage during PDT and enhance therapeutic efficacy against hypoxic solid tumors.
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JOURNAL OF FLUORESCENCE
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2026
·
IF 3.4
Hou Huijing,Luo Mingming,Li Fang,Tan Yijun,Liu Ruowei,Xu Longping,Feng Yanwen,Guo Xiangli,Wu Zijian
Kanamycin (KANA) is a veterinary drug residue that is highly scrutinized in food safety testing. In this study, a metal-organic framework-based aptamer-nanoenzyme (NH 2 -MIL-88B(Fe)/CDs-Apt) was introduced for the dual-mode detection of KANA. NH 2 -MIL-88B(Fe) catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H₂O₂ to form blue ox-TMB, thereby enabling colorimetric detection. As a fluorescence quencher, NH 2 -MIL-88B(Fe) quenches the fluorescence of CDs via photogenerated electron transfer (PET) or fluorescence resonance energy transfer (FRET) mechanisms. The detection limits were 0.716 fM (fluorescent) and 0.709 fM (colorimetric), respectively. The proposed aptasensor was successfully applied to the detection of KANA in eggs, honey, and lake water. This aptasensor demonstrates high sensitivity and accuracy, as well as broad application potential.
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Nanomaterials
·
2026
·Vol. 16
·期 6
·378
·
IF 4.8
Hao Li,Lin Zhang,You Long,Chao Shen,Song Zhang,Fang Chen,Nan Chen,Chenghong Huang
Paclitaxel is a first-line anticancer drug, but its low water solubility impedes bioavailability. The purpose of this study is to estalish a delivery strategy via carboxymethyl chitosan (CMCS)-encapsulated 6-deoxy-6-mercapto-β-cyclodextrins (dmβCDs)-modified concave cubic gold (CCGs) to achieve PTX release. CCGs were initially synthesized by the one-pot method and further modified by dmβCDs, the dmβCDs can effectively capture PTX molecules, followed by encapsulation with CMCS, and then prepare pH-responsive CMCS/dmβCDs/CCGs nanocarriers after lyophilization. Results indicated that desirable hexagonal CCGs with 50 ± 5 nm size can be obtained by adjusting H 2 O 2 and HClO concentration. FT-IR, Raman and XRD spectra had confirmed dmβCDs successfully grafted to the surface of CCGs. Drug loading experiments demonstrated that the nanocarrier encapsulated PTX in amorphous powder or molecular form have a capacity of 55.05 µg/mL. Drug release experiments revealed PTX release from CMCS/dmβCDs/CCGs nanocarriers carrying a typical pH-responsive profile and indicating earlier release in an acidic environment than in a neutral or alkaline environment. The proposed method can be utilized to effectually achieve high-efficiency solubilization and targeted release inside tumor cells of PTX.
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PROSTAGLANDINS & OTHER LIPID MEDIATORS
·
2026
·
IF 2.6
Lili Liu,Lijuan Xue,Guiyue Hou,Jingxuan Zhang,Ling Tian,Xueqin Wang,Ronghua Dai
Inflammation initiates and progresses due to overexpression of the arachidonic acid cascade mediators. Cyclooxygenase (COX) and lipoxygenase (LOX) are considered to be the primary targets, and the aim of this study was to assess the anti- inflammatory activity of several herbal compounds as dual COX-LOX inhibitors on prostatic diseases. Compounds Timosaponin AⅡ, timosaponin AⅢ, berberine and demethyleneberberine were selected according to our previous studies. Human prostate cancer cells (PC-3) were cultured and the cell viability was detected by CCK-8 method at different concentrations. Ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was applied to monitor the changes of AA metabolites after treatment. Western blot and qPCR were used to detect COX-2 and LOX-5 at protein and mRNA levels, respectively. Additionally, the levels of inflammatory factors (IL-6, IL-1β, TNF-α) in each group were detected by ELISA. After drug interference, 13 altered metabolites associated with the COX/LOX pathway, including PGE 2 , 5-HETE, LTB 4 , etc. were identified in cell medium. Quantitative metabolomics analysis showed that the candidate compounds could significantly decreased the concentrations of dual target related metabolites to varying degrees ( P < 0.01). These compounds could also suppressed COX-2 and LOX-5 expression at the protein and mRNA levels simultaneously. Moreover, the levels of cytokine IL-6, IL-1β and TNF-α were also significantly reduced in the treated group compared to controls ( P < 0.05 or P < 0.01). Our findings revealed the four active compounds were potential COX/LOX dual-target inhibitors, which inhibited a range of inflammatory responses by interfering with AA metabolism and down-regulating the levels of COX and LOX metabolites.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Bingbing Liu,Lin Jiang,Lvyang Lin,Wulong Yang,Mian Li,Jianping Lin,Yiqi Jiang,Zehua Bao,Mianbin Wu
Xylitol is industrially synthesized by d-xylose, which is more expensive than glucose. However, de novo xylitol biosynthesis from glucose remains challenging in Escherichia coli. To address these challenges, this study first developed a biosynthetic pathway from glucose via an arabitol intermediate with arabitol phosphate dehydrogenase (APDH). Chassis optimization through knocking out key genes (rpiA/B, pgi, tktA, tktB, xylB) enhanced flux and increased arabitol yield to 1.61 g/L. Subsequently, the arabitol-to-xylitol route was established using arabitol dehydrogenase (ArDH2) and xylitol dehydrogenase (XDH1) enzymes, showing a 52.1% conversion rate in vitro. The heterologous AXA module (APDH, ArDH2, XDH1) was multicopy integrated into the optimized chassis W3P-5ΔB. The strain W3P4A produced 5.04 g/L arabitol and 1.89 g/L xylitol in shake-flasks. Fed-batch fermentation with a plasmid-carrying strain achieved 5.11 g/L xylitol and 18.5% (g/g) yield from glucose. This work establishes a new platform for xylitol bioproduction from glucose and provides a foundational framework for industrial-scale implementation.
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World Journal of Surgical Oncology
·
2026
·
IF 2.8
Wang Ju,Zhang Yong,Li Heyu,Peng Jikui,Li Haijun,Gao Xiaoping
STIL is a key molecule influencing the proliferation and cell cycle of gastric cancer cells; however, STIL is also expressed in normal tissues. Therefore, designing a drug delivery system that targets gastric cancer cells is crucial for STIL-targeted therapy. In this study, we constructed exosomes targeting the HER-2 receptor and loaded with siSTIL (Anti-HER-2-Exo-siSTIL), and validated the inhibitory effects of Anti-HER-2-Exo-siSTIL on gastric cancer proliferation and metastasis in vivo. Anti-HER-2-Exo-siSTIL was able to enter SGC-7901 cells and inhibit STIL expression, significantly suppressing the growth of SGC-7901 xenograft tumors. By downregulating STIL and the anti-apoptotic protein Bcl-2, and upregulating the pro-apoptotic proteins Bax, Caspase-3, and Caspase-9, Anti-HER-2-Exo-siSTIL effectively induced apoptosis in tumor cells. Furthermore, Anti-HER-2-Exo-siSTIL selectively inhibited lung metastasis of SGC-7901 cells via hematogenous dissemination. In summary, Anti-HER-2-Exo-siSTIL specifically targets and suppresses gastric cancer proliferation and lung metastasis, demonstrating its significant translational potential for gastric cancer therapy.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Jiacheng Dong,Yu Zhang,Yanhua Rao,Jiabao Yang,Duoshuang Xu,Yongteng Long,Ying Liu,Nannan Liu
The ultrasensitive detection of kanamycin is important for acidic-condition analytical sensing. However, pH variations in acidic samples often trigger significant background interference, leading to false positive or negative results. In this study, a machine learning-assisted dual-probe nanoconfined sensor (KBA&C4/SiNWs/GMP) was developed for the precise identification of kanamycin under acidic fluctuations. By co-functionalizing a glass micropipette (GMP) filled with silica nanowires (SiNWs) with a kanamycin-binding aptamer (KBA) and a proton-responsive DNA strand (C4 DNA), the sensing interface integrates target recognition with pH-responsive interfacial regulation. To enhance recognition reliability, machine learning algorithms, including Support Vector Machine (SVM), K-Nearest Neighbors (KNN), and Linear Discriminant Analysis (LDA), were employed to classify the ionic current responses. The machine learning models accurately classified concentration-dependent ionic current responses despite signal variations caused by acidity fluctuations, enabling the sensor to maintain a low limit of detection (7.31 fM) and a high classification accuracy (94.17%) in acidic matrices. This work provides a promising strategy for reliable kanamycin detection in acidic and complex sample environments.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 7.7
Huikai Lin,Caiyi Du,Ningyi Xu,Yiru Wang,Chunyan Sun
CRISPR/Cas12a can activate trans-cleavage activity upon single-stranded DNA binding, making it widely applicable in highly sensitive fluorescent biosensors. Sensors utilizing monochromatic fluorescence output modes are susceptible to interference from multiple factors, resulting in unstable detection methods. To address this, we designed a ratio-based fluorescence sensor for detecting tetracyclines (TCs) based on the synergistic signal amplification of nucleic acid aptamer (42TET) and zirconium-based metal-organic framework (PCN-224) with CRISPR/Cas12a. PCN-224 adsorbs single-stranded DNA, quenching its FAM-labeled fluorescence while simultaneously emitting its own fluorescent signal. This dual-fluorescence output reduces background interference and enhances detection accuracy. By designing an activator as a complementary sequence (cDNA) to the nucleic acid aptamer, the system binds to 42TET in the presence of tetracycline, releasing the cDNA to activate the CRISPR/Cas12a system. Through this system's signal amplification capability, highly sensitive detection of tetracycline is achieved, with a detection limit reaching 8.7 nM. It possesses the same detection capabilities as traditional HPLC detection methods. This method is simple, rapid, efficient, and highly quantitative, offering broad prospects for future applications.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2026
·Vol. 132
·期 3
·226
·
IF 3
Hong Khanh Trung,Dang Huu Phuc,Le Hanh Ngoc Thi,Dieu Nguyen,Duyen Ky,Nguyen Quoc Thang,Nguyen Van Cuong
This study introduces a rapid synthesis method for nitrogen-doped carbon quantum dots (N-CQDs) using microwave irradiation, with citric acid and urea serving as the carbon and nitrogen sources, respectively. The material was characterized by Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) spectroscopy, and fluorescence spectroscopy, revealing a uniform structure, nanoscale size (4–10 nm), and excellent optical properties. N-CQDs were used as fluorescent sensors to detect Fe³⁺ ions and oxytetracycline (OTC) with high sensitivity and selectivity. The results demonstrated limits of detection (LOD) of 1.685 µM and 0.638 µM for Fe³⁺ and OTC, respectively, within linear ranges of 1–32 µM and 0.2–100 µM, respectively. The fluorescence quenching mechanism for Fe³⁺ was attributed to static quenching, whereas OTC enhanced the fluorescence via hydrogen bonding and π–π interactions. The analytical performance of the N-CQDs surpassed that of many previously reported methods, highlighting their potential for environmental sensing and antibiotic residue monitoring.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Bin Yan,Hongru Zhao,Bingmei Su,Juan Lin
Protein engineering altered the reaction direction of TsRTA for AP. • Adjusting the catalytic center and entrance of TsRTA promoted AP binding. • A 10-site variant of TsRTA enabled biosynthesis of ( R )-1-PEA. • An acetone elimination module increased Mu10 productivity by 57.4%. ( R )-1-Phenylethylamine [( R )-1-PEA] is an important chiral compound widely used in pharmaceuticals, chemical synthesis, and materials science. ω-Transaminases are valuable biocatalysts for the asymmetric amination of ketone substrates under mild conditions and represent an attractive tool for the green synthesis of chiral amines. However, in natural ( R )-selective transaminases, acetophenone (AP) is more commonly produced as the deamination product of ( R )-1-PEA, whereas its amination as an amino acceptor is generally inefficient. In this study, an ( R )-selective ω-transaminase from Thermomyces stellatus (TsRTA), which originally catalyzed only the deamination of ( R )-1-PEA to AP, was selected as the engineering target. Protein engineering was performed to shift its reaction equilibrium, thereby enabling the asymmetric synthesis of ( R )-1-PEA from AP. To overcome the thermodynamic limitation caused by acetone accumulation, an “acetone elimination–cofactor regeneration” coupled module was introduced to improve the whole-cell catalytic efficiency for ( R )-1-PEA synthesis using AP as the substrate. Under optimal reaction conditions (35 °C, pH 10.0, 0.5 mM PLP, an isopropylamine/AP molar ratio of 4:1, 200 mM formate, and 0.2 mM NAD + ), the whole-cell catalytic system produced 67 mM ( R )-1-PEA from 100 mM AP within 8 h. This study lays a foundation for the scalable preparation of ( R )-1-PEA and provides a useful reference for regulating the reaction direction of transaminases. Download: Download high-res image (205KB) Download: Download full-size image
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ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 26
·2213471
·
IF 19
Ming Yi,Thi Dao Nguyen,Huan Liu,Yafang Liu,Shu Xiong,Yan Wang
Membrane decorated with biocides is an effective way to suppress biofilm growth. However, their immediate biocidal effect usually suffers from a significant decline due to the irreversible consumption of the biocides. Here, a smart nanofiltration membrane is reported with rechargeable antibacterial capability that is fabricated by a facile interfacial polymerization via 3-aminophenylboronic acid and trimesoyl chloride on a polysulfone substrate. Biocides bearing diol groups can be grafted onto the membrane surface under neutral/alkaline condition and then released from the surface under acidic environment, due to the pH-responsive feature of boronate ester complexes. The resultant membrane exhibits integrated properties of fast bacterial inactivating efficiency, rechargeable antibacterial capability, and impressive stability. In addition, the achieved membrane shows remarkable separation efficiency to dye/monovalent salt system. The successful fabrication of the membrane with rechargeable anti-bacterial property provides new insights into the development of pH-responsive and sustainable antibacterial membranes.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Wanling Cui,Jinrong Liu,Wenqi Zhao,Jiayi Zhang,Yanyan Wang,Qiang Li,Rui Wang,Mei Qiao,Shicai Xu
Kanamycin residues accumulate in the human body and endanger human health, so the sensitive detection of kanamycin has important research significance. Herein, we constructed an enzyme-free and label-free fluorescent aptasensor for sensitive detection of kanamycin in milk samples based on hybridization chain reaction. We first designed a kanamycin recognition probe with a blocked aptamer and a blocked trigger sequence. Kanamycin specifically recognized the aptamer and then initiated the conformational transition process of the recognition probe to generate an aptamer-kanamycin complex with an activated trigger sequence. The trigger sequence initiated alternate hybridization of two partially complementary assembled probes to generate a long double-stranded DNA with many intact G-quadruplex structures for amplified, enzyme-free detection of kanamycin. The fluorescent dye N-methylmesoporphyrin IX was inserted into the intact G-quadruplex structures, and then it emitted a strong fluorescent signal for amplified, label-free detection of kanamycin. The linear range was from 2.0 to 200 nM for kanamycin, and the limit of detection (LOD), limit of quantification (LOQ) were 0.562 nM and 1.855 nM respectively. Moreover, the fluorescent aptasensor presented high selectivity for kanamycin compared with other antibiotics. Furthermore, the recoveries of spiked kanamycin in milk samples ranged from 98.66% to 100.7%, indicating that the fluorescent aptasensor had a great application prospect in food safety detection.
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