ACS Nano
·
2023
·
IF 17.1
Yusheng Zhang,Xiaoyang Wu,Jie Ding,Borui Su,Zhihong Chen,Zhanwen Xiao,Chengheng Wu,Dan Wei,Jing Sun,Fang Luo,Huabing Yin,Hongsong Fan
Electrical deep brain stimulation (DBS) is a top priority for pharmacoresistant epilepsy treatment, while less-invasive wireless DBS is an urgent priority but challenging. Herein, we developed a conceptual wireless DBS platform to realize local electric stimulation via 1D-structured magnetoelectric Fe3O4@BaTiO3 nanochains (FBC). The FBC was facilely synthesized via magnetic-assisted interface coassembly, possessing a higher electrical output by inducing larger local strain from the anisotropic structure and strain coherence. Subsequently, wireless magnetoelectric neuromodulation in vitro was synergistically achieved by voltage-gated ion channels and to a lesser extent, the mechanosensitive ion channels. Furthermore, FBC less-invasively injected into the anterior nucleus of the thalamus (ANT) obviously inhibited acute and continuous seizures under magnetic loading, exhibiting excellent therapeutic effects in suppressing both high voltage electroencephalogram signals propagation and behavioral seizure stage and neuroprotection of the hippocampus mediated via the Papez circuit similar to conventional wired-in DBS. This work establishes an advanced antiepilepsy strategy and provides a perspective for other neurological disorder treatment.
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SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Amin Zhang,Chongchong Miao,Hongyuan Shi,Hongkun Xiang,Chusen Huang,Nengqin Jia
Recent years have witnessed the great development of electrochemiluminescence (ECL) as a platform for the analytical processes in many fields, which includes pharmaceutical analysis, immunoassay, DNA analysis as well as clinical analysis. Herein, a novel electrochemiluminescence (ECL) sensor was fabricated for ultrasensitive detection of atropine based on Ru(bpy) 3 2+ /carbon nanospheres/Nafion composite film. The carbon nanospheres (CNPs) in composite film were obtained through a hydrothermal method. Then, electrochemical and ECL behaviors of the proposed sensor were investigated. Under the optimal conditions, the constructed atropine sensor showed a wide linear range from 0.5 to 250 μM with a detection limit of 0.17 μM (at signal-to-noise ratio of 3). Moreover, the results indicated that the CNPs-based ECL sensor exhibited excellent sensitivity and favorable stability, which might be ascribed to the high surface area and the good electrical conductivity of CNPs. Furthermore, the ECL sensor was applied for the determination of atropine in atropine sulfate injection samples and displayed a desirable result. The proposed ECL sensor could be easily obtained and be promising used for drug assay.
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FORENSIC SCIENCE INTERNATIONAL
·
2013
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IF 2.307
Liang Meng,Pinjia Meng,Bugang Tang,Qingqing Zhang,Yanji Wang
Based on molecularly imprinted photonic hydrogels (MIPHs) that combined the colloidal-crystal with molecular imprinting technique, a novel label-free colorimetric chemosensor for convenient and fast efficient detection of atropine with high sensitivity and specificity was developed. Due to the special inverse opal arrays with a thin polymer wall in which the imprinted nanocavities of atropine moleculars distributed, the proposed MIPHs designed as water-compatible exhibited high sensitive (as low as 1 pg/mL), rapid responsive (less than 30 s) and specific detection of atropine in complex matrix. The unique three-dimensional, highly-ordered photonic hydrogels would be obviously swelling in response to the specific atropine molecular recognition process and the response would be directly transferred into visually perceptible optical signal (change in color) that could be detected by the naked eye through Bragg diffractive shifts of ordered macroporous arrays. With a broad concentration range varying from 1 pg/mL to 1 μg/mL of atropine, the distinct color changes of MIPHs almost covered the whole visible-light wavelength range from blue to red for semi-quantitative analysis. The smart chemosensor was successfully employed to determine the trace level atropine in human urine samples, providing a fast and effective alternative for semi-quantitative detection of atropine for clinical analysis and forensic investigations.
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RESEARCH IN VETERINARY SCIENCE
·
2025
·
IF 1.8
Zhiqin Liu,Yufeng Xu,Wei Liu,Lin Wang,Zhen Dong,Jianguo Zeng
Diarrhoea remains a major public health concern, particularly affecting young children and livestock. Macleaya cordata protopine total alkaloids (MPTA), a standardized extract approved in China for poultry diarrhoea, has demonstrated anti-inflammatory properties in intestinal disorders. The study aims to investigate the antidiarrheal mechanism of MPTA using castor oil- and E. coli -induced diarrhoea models in mice. We first tested MPTA for acute oral toxicity. Subsequently, the effect of MPTA on castor oil- and E. coli -induced diarrhoea in mice based on LD 50 results. Network pharmacology analysis and target competition assays (inhibitors and antagonists) were integrated to identify targets for MPTA's antidiarrheal effects. Molecular docking was used to verify the binding ability of MPTA components to these receptors. The LD 50 of MPTA was determined to be 426.1 mg/kg. The optimal MPTA activity was found at 8 mg/kg in both castor oil and in infectious models. Network pharmacology analysis revealed potential targets and pathways of MPTA against intestinal motility. The impact of MPTA on cholinergic, serotonin, dopaminergic, and adrenergic receptors was assessed using standard inhibitors and agonists to induce intestinal smooth muscle contractions or relaxations. Molecular docking confirmed the binding ability of MPTA components to these receptors. In conclusion, MPTA exhibits significant antidiarrheal effects in both castor oil and E. coli -induced diarrhoea models. Its mechanism may involve modulation of cholinergic, serotonin, dopaminergic, and adrenergic receptors, as well as inhibition of ion channels and anti-inflammatory actions. These findings highlight the potential of MPTA as a novel therapeutic agent for diarrhoea.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Jinqiu Xu,Peipei Lu,Liangliang Cai,Wenyi Wu,Qing Zhu,Xiaofei Ma
In this work, we synthesized a novel type of Y-shaped nanomicelles via an esterification reaction, with γ-cyclodextrin (γ-CD) as the hydrophilic group and branched long chain carboxylic acids as the hydrophobic groups. These ester compounds can self-assemble into nanomicelles in aqueous solution, simultaneously serving as a chiral selector and pseudostationary phase in capillary electrophoresis (CE) for enantioseparation. The Y-shaped nanomicelles were systematically characterized using fourier transform infrared spectroscopy, nuclear magnetic resonance (NMR), particle size analysis, scanning electron microscope (SEM), transmission electron microscope (TEM), foaming effect and Tyndall effect. Due to the presence of branched chains, the hydrophobic chains of the Y-shaped nanomicelles exhibited increased hydrophobicity, and the hydrophobic interaction between the long chains in the cores was also enhanced. As a result, the stability of the Y-shaped nanomicelles was higher, reducing their tendency to dissociate. They also demonstrated a superior drug loading capability, thereby more effectively serving as a pseudostationary phase. Compared to the single γ-CD system and conventional nanomicelles system, the separation of nine model drugs in the Y-shaped nanomicelles system was significantly improved. We used racemic promethazine as a model drug and studied the drug loading of native γ-CD, conventional nanomicelles, and Y-shaped nanomicelles through high performance liquid chromatography. Additionally, the encapsulation effect was investigated using several methods such as particle size analysis, TEM, NMR, elemental analysis and thermogravimetric analysis. These results confirmed a higher distribution of enantiomers in the Y-shaped nanomicelles. This study demonstrates the superiority of Y-shaped nanomicelles in CE for enantioseparation and represents an important step toward the development of novel chiral recognition materials.
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ADVANCED MATERIALS
·
2025
·Vol. 37
·期 19
·2415353
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IF 26.8
Ming Shao,Yu Chai,Yutong Jiang,Xinyi Wu,Wenjie Xie,Jiayi Lu,Xuehui Fu,Yao He,Xiaofeng Zhang,Han Zhang,Zhuang Liu
Dry eye disease (DED), the most prevalent ophthalmological condition worldwide, can cause severe ocular discomfort and even visual impairment. Effective yet safe therapeutics for severe DED are still highly demanded. Herein, considering the important role of excessive reactive oxygen species (ROS) in triggering DED, an eye-drop nano-formulation of catalase (CAT) self-assembled with cysteine-modified chitosan (CS-Cys) is designed for DED treatment. Upon eye-drop administration of CS-Cys/CAT nanoparticles, CS-Cys can form disulfide bonds with abundant thiols in the mucin layer of the tear film, anchoring catalase to the corneal surface. Thus the excess ROS accumulated on the ocular surface can be effectively eliminated, resulting in a regulated tear microenvironment. In mouse and rabbit models, it is verified that CS-Cys/CAT eye drops can offer excellent therapeutic effects, especially in promoting the recovery of damaged epithelium and increasing tear secretion. Remarkably, CS-Cys/CAT eye drops showed notably better therapeutic performance than clinically used cyclosporin and dexamethasone, as well as several new DED drugs in clinical trials. Thus, the work presents a unique nanoparticulate eye-drop-based ocular delivery system to allow prolonged ocular retention of protein therapeutics, and such nanoformulation formulated by fully biocompatible/biodegradable components possesses significant translational potential for effective and safe DED treatment.
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