Small
·
2013
·Vol. 10
·期 7
·1379-1386
·
IF 7.823
Yuting Zhang,Wanfu Ma,Dian Li,Meng Yu,Jia Guo,Changchun Wang
Efficient enrichment of specific glycoproteins from complex biological samples is of great importance towards the discovery of disease biomarkers in biological systems. Recently, phenylboronic acid-based functional materials have been widely used for enrichment of glycoproteins. However, such enrichment was mainly carried out under alkaline conditions, which is different to the status of glycoproteins in neutral physiological conditions and may cause some unpredictable degradation. In this study, on-demand neutral enrichment of glycoproteins from crude biological samples is accomplished by utilizing the reversible interaction between the cis -diols of glycoproteins and benzoboroxole-functionalized magnetic composite microspheres (Fe 3 O 4 /PAA-AOPB). The Fe 3 O 4 /PAA-AOPB composite microspheres are deliberately designed and constructed with a high-magnetic-response magnetic supraparticle (MSP) core and a crosslinked poly(acrylic acid) (PAA) shell anchoring abundant benzoboroxole functional groups on the surface. These nanocomposites possessed many merits, such as large enrichment capacity (93.9 mg/g, protein/beads), low non-specific adsorption, quick enrichment process (10 min) and magnetic separation speed (20 s), and high recovery efficiency. Furthermore, the as-prepared Fe 3 O 4 /PAA-AOPB microspheres display high selectivity to glycoproteins even in the E. coli lysate or fetal bovine serum, showing great potential in the identify of low-abundance glycoproteins as biomarkers in real complex biological systems for clinical diagnoses.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 17
·11025-11032
·
IF 12.732
Zhiwei Guo,Chunyang Bao,Xiaohan Wang,Xingyuan Lu,Haoxiang Sun,Xiang Li,Jian Li,Junqi Sun
Mechanically robust polyurethanes and polyureas with excellent healing and recycling capacities have an extended service life, and can reduce raw material consumption and environmental pollution. In this work, healable, recyclable and mechanically super-strong poly(urea-urethane)s (PUUs) are conveniently fabricated via cross-linking isocyanate-terminated poly(tetramethylene ether glycol) (PTMEG) with nitrogen-coordinated boroxines (NCBs). The tripodal NCBs generate three-dimensionally cross-linked PUUs (denoted as NCB-PUUs) with a high cross-linking density. Meanwhile, hydrogen bonds and phase-separated PTMEG segments can serve as physical cross-linkers to further strengthen NCB-PUUs. As a result, the NCB-PUUs exhibit a tensile strength of ∼47 MPa and a toughness of ∼190 MJ m−3. Because of the high reversibility of NCBs and hydrogen bonds, the NCB-PUUs are capable of efficiently healing and recycling at room temperature with the assistance of ethanol or a water/ethanol mixture to restore their original mechanical strength. NCB-PUUs can be conveniently deposited on solid surfaces by dip-coating and spraying methods to serve as protecting coatings. The NCB-PUU coatings have firm adhesion on solid surfaces and can be doped with organic dyes to display diverse colors. More importantly, the NCB-PUU coatings can heal deep and wide cuts and scratches for multiple times in a given region.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 137
·期 20
·48703
·
IF 2.188
Chun Zhang,Yongping Bai,Wenwen Liu
4-formyl-phenylboronic acid (FBA) and caffeic acid (CA) can combine to obtain a novel vinyl monomer containing boroxin, which was named as FBCA. Having been reduced the inhibition of phenolic hydroxyl groups, FBCA could directly copolymerize with acrylate (for instance, isobornyl acrylate (IBOA), butyl acrylate (BA), and hydroxypropyl methacrylate (HPMA)) by free-radical polymerization to produce modified acrylate copolymer adhesive (FBCA-IBH). Compared with sole acrylate copolymer adhesive (IBH), the FBCA-IBH adhesive presents a higher thermal stability, cohesive strength, creep resistance, strain recovery, and adhesion strength to different substrate (such as carbon steel, polypropylene (PP), and polytetrafluoroethylene (PTFE)). According to the results of TGA, the initial degradation temperature of FBCA-IBH is increased by 50 °C. The creep resistance of FBCA-IBH is improved and its holding power to carbon steel is increased from 19 min to 3 h. It is eye catching that the shear strength to carbon steel could reach 9.37 MPa. Besides, this shear strength can be maintained even if after 18 cycle times, even the maximum value can achieve 14.36 MPa. The adhesion of thermoplastic adhesives to carbon steel exhibits adhesion strength comparable to that of thermoset adhesives. The reversible adhesive presents bonding strength in macro-field, not just limited in micro-field. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 137 , 48703.
研究领域:
材料科学
·
聚合物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Xinzhao Luo,Xin Liu,Yaqi Dong,Liyuan Fan,Tianheng Wang,Qiang Zhang
As a kind of functional heterocyclic polymer, the boroxine network could be facilely synthesized via dehydration reaction. However, due to the reversible nature of the dynamic covalent boroxine bond in water, it has rarely been used to fabricate membrane materials. Herein, we report the fabrication of selective layer of anti-dye-deposition membranes based on stable boroxine network for the first time. Functional polymers with pendant phenylboronic acid groups were dehydrated under mild conditions to form stable cross-linked boroxine network, which successfully acted as the selective layer of membranes. The pore size of selective layer could be well-tuned by changing the reaction conditions such as concentration, temperature, and vacuum degree. Compared with the original membrane, the composite membranes exhibit high separation performance towards organic molecules. In addition, the boroxine network demonstrated excellent chemical stability in water and could maintain stable permeation flux and retention rate after 30 days of operation. In particular, the modified membranes exhibited unexpected anti-fouling performance against organic dye clusters. After repeated anti-fouling cycles, the flux recovery rate of modified membranes was above 99.2%, highlighting the potential of the boroxine network in enhancing the anti-fouling performance of membrane.
研究领域:
材料科学
·
膜技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·Vol. 43
·期 1
·177-187
·
IF 4.1
Wu Hao-Min,Ma Wen-Can,Cai Yi-Feng,Huang Xin,Li Jun-Heng,Kai Xi,Zhang Qiu-Hong,Jia Xu-Dong
Solid-state electrolytes are considered to be the vital part of the next-generation solid-state batteries (SSBs), due to their high safety and long operation life span. However, the two major factors that impede the expected performance of batteries are: the easy formation of lithium dendrites due to the concentration gradient of anions, and the low ionic conductivity at room temperature, which prevents reaching ideal electrochemical performance. Single-ion quasi-solid-state electrolytes (SIQSSEs) could provide higher safety and energy density, owing to absence of anion concentration gradient and solvent, as well as good lithium-ion transport ability. The porous covalent organic frameworks (COFs) are beneficial for con-structing appropriate lithium-ion transport pathway, due to the ordered 1D channel. In addition, the boroxine COFs (COF-5) offers strong ability of withdrawing anion part of lithium salt. Last but not the least, boron atom could play the role of coordinate site due to its electron deficiency. These advantages afford an opportunity to obtain a SIQSSE with high ionic conductivity and high lithium transference number (LTN) simultaneously. The COF-5 based SIQSSEs delivered a high ionic conductivity of 6.3×10 −4 S·cm −1 , with a high LTN of 0.92 and a wide electrochemical stable window (ESW) of 4.7 V at room temperature. The LiFePO 4 (LFP)/Li cells, which was assembled with COF-5 based SIQSSE, exhibited outstanding long cycle stability, high initial capacity and favorable rate performance. The results indicated COFs could be an ideal material for single-ion solid-state electrolytes in next-generation batteries.
研究领域:
材料科学
·
能源存储
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Hengye Li,Xuemeng Zhang,Lin Zhang,Weihua Cheng,Fenying Kong,Dahe Fan,Lei Li,Wei Wang
4-Carboxy-benzoboroxole was designed and synthesized. It was then combined with the modification effect of polyethyleneimine (PEI) for the preparation of boronate affinity silica stationary phase. The stationary phase showed improved binding strength with dissociation constant ( K d ) towards xanthosine as low as 2.48 × 10 −4 M. The column showed excellent selectivity , high binding capacities (88.3 μmol adenosine g −1 , pH 7.0) and the lowest binding pH (4.0 for cytidine and as low as 2.24 for xanthosine). These binding properties were superior to the existing boronate affinity materials, facilitating the selective extraction of trace cis -diol compounds in complex samples and greatly expanding the application scope of boronate affinity chromatography . In addition, the column showed secondary separation capability under acidic conditions and this secondary separation capability was investigated thoroughly. It was found that the separation was pH-dependent and mainly determined by binding strength with the possibility of involvement of other interaction, providing alternative strategy for the separation of cis -diol compounds. The feasibility and practicability were demonstrated through the selective enrichment of nucleosides in urine samples and the results indicated the excellent performance and great potential for the extraction of trace cis -diol compounds in complex samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 34
·8228-8240
·
IF 7
Weiwei Fei,Xiuli Wang,Jia Guo,Changchun Wang
The design of targeting agent-conjugated systems is attracting much attention in cell targeted delivery and cancer therapy. However, quantitative study of the ligand density and binding efficiency is still limited due to the technical matters and tedious work involved. In this article, benzoboroxole-modified core–shell magnetic nanoparticles (MSP-AOPB NPs) as a drug carrier model were fabricated and transferrin (Tf) was immobilized on the nanoparticle surface in a site-oriented manner (Tf-MSP-AOPB NPs). The preparation conditions were investigated in detail to optimize the Tf binding efficiency. A suitable reaction temperature, time or initial feeding amount could significantly increase the Tf binding amount. The maximum Tf binding amount on the MSP-AOPB NPs was 184 mg g−1, and the targeting ligand density on the surface could be well controlled by simply adjusting the reaction conditions. In vitro studies demonstrated the excellent Tf-mediated targeting ability and enhanced cellular uptake efficacy by varying the ligand density. The optimal ligand binding amount for achieving the highest cellular uptake efficiency was 94 mg Tf/g, which corresponds to a ligand binding density of about 0.05 Tf/nm2, and the binding efficiency of conjugation was higher than 90%. Moreover, Tf-MSP-AOPB NPs prepared by a site-oriented conjugation strategy showed the best cell targeting ability, and their cellular uptake amount was 25 and 127 times higher than that of physical adsorption and EDC/NHS coupling reaction in HepG2 cells, respectively. This study provides a facile site-oriented bioconjugation technique for different kinds of antibodies, and a suitable ligand density can be easily attained to enhance the cellular uptake efficacy, which shows great significance for targeted delivery and cancer therapy.
研究领域:
纳米技术
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2022
·
IF 7.613
R. Longfang,L. Congcong,Q. Taotao
Based on the hydrolysis and dehydration of boroxine and boric acid, a boroxine was prepared from 3-Aminobenzeneboronic acid (3-ABBA). Then a novel boroxine-based self-healing polyurethane (BPU) which has a repeatable adhesive property under mild conditions was synthesized by using boroxine and polyurethane prepolymer . The chemical structure and thermal property were confirmed by Fourier transform infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA). When the mole ratio of isophorone diisocyanate (IPDI) and polypropylene glycol 2000 (PPG 2000) was 3:0, the mechanical strength and elongation at break of BPU film reached 1.63 MPa and 1577.79%. Meantime, BPU could be healed under the action of a water molecule and the self-healing rate was up to 92.02%. BPU film took on excellent self-healing property under mild conditions. In addition, BPU film could easily dissolve in N, N-dimethylacetamide (DMAC) to become an adhesive. The bonding strength of BPU adhesive to leather substrate reached 1.73 MPa, which was nearly the same as the standard value of adhesive used for shoes and bags. Interestingly, after the bonded leather was separated into two parts, they can adhere together again by spraying water on the bonding part. Through healing the bonding strength of BPU adhesive was still 1.65 MPa. Therefore, BPU is characterized by excellent repeatable adhesive property which has great potential application.
研究领域:
材料科学
·
高分子化学
·
粘合技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）